Click here to switch to the map view.

The map label for this gene is mrdA [H]

Identifier: 238919647

GI number: 238919647

Start: 1699679

End: 1701592

Strand: Direct

Name: mrdA [H]

Synonym: NT01EI_1748

Alternate gene names: 238919647

Gene position: 1699679-1701592 (Clockwise)

Preceding gene: 238919646

Following gene: 238919651

Centisome position: 44.58

GC content: 59.61

Gene sequence:

>1914_bases
ATGAATTTGTTAAAAGACGCCATTCGCGATCATGGTGCCGAGTCGGCGCTGTTTCTGCGCCGCACGCTGATTGCGCTGGT
ACTGGTGCTCCTGTGCTTCACTGTACTGGTCGTTAACCTATACCACCTTCAGGTGCAGGAGCACGCCGCATATCAGACCC
GTTCGGATGCGAACGACATCAAAACTCTGCCAGTGTCGCCGGTGCGCGGGCTGATTTACGATCGCCACGGTACGCCGCTG
GTGGAAAACCAGACGCTATACCAGATTTCGCTGGTGCCGGGGAAGATCCCCCATCTTGATCAGACGCTGACGGCGCTGAC
ACCGTTGTTGGACCTGACGCCTCAGGAGATTGCCGATTTTCATCAGGCGATGAAACACGCACGTCGTTTTGCCGAGGTTA
CCCTGAAAGAGGGGCTGACTGAGCGCGAGGTGTCCCGGTTTGCGGTCAACGAATTTGCTTTTCCCGGGGTGATGCTCTCG
ACCTACCAGCAGCGTTATTATCCTTATGGCGCACAGCTGGCGCATGTGGTTGGCTATGTGTCTAAAATCAACGATCGCGA
TCTGCAGCGCCTTGATGCCGAAGGACAGCGGGAAAACTATGCCGCCGACCACGATATCGGTAAACAGGGGATCGAGCGTT
ATTACGAATCGCTGCTGCACGGCAAGACCGGCAGTCAAGAGGTCGAGGTTGACAGTCACGGGCGGATAGTGCGCTCTTTG
AAAGAGCAGCCGCCCCACGCCGGCGCCAGCCTGTATCTGACACTGGATCTGCCGCTACAGCAATATATTGAGGGGGTACT
GAAAGGGCAGCGGGCGGCGGTGGTGGTGATGGATCCGCGCGATGGCGGCATTCTGGCCATGGTCTCCAGTCCCAGCTACG
ATCCCAACCCCTTCGTTAACGGGATTTCCTATAAGGCCTATAGCGCGCTGCTGCATGATACGGCGCTGCCGCTGATTAAC
CGCGTGACCCAGGGGATCTACCCTCCGGCCTCGACGGTGAAACCCTATATGGCCAGCGCGGCGCTGCTGGCGGGGGTGAT
CACCCCGCAGACGACCTTCTTCGGCGCCCCGACCTGGACGCTGCCCGGTACCCAGCGCCGTTACCGAGACTGGCTGAAGA
GCGGGCACGGGATATTGAATGTGGTGAAGGCCATTGAGGAGTCGGCGGATACCTTCTTTTACCAGGTTGCCTATGAGATG
GGGATCGATCGCATCCACCAGTGGATGAGCCAGTTTGGCTACGGTCAGCTGTCCGGCATCGATCTGGATGAAGAGGCACG
TGGGGTCTTACCCGATCGCGACTGGAAGATGCGGGTGCATCATAAGCCCTGGTACCAGGGGGATACGGTATCCGTCGGCA
TCGGGCAGGGGTATTGGACGGCGACGCCGATTCAGATGGTGAAAGCGCTGGGGATCCTGATTAACAACGGCAAAGTGGTG
ACGCCGCACCTGCTGTACGCCGTCCGCCGCGGCCAGCAGGTCGTGCCCTACCGCCAGAGCCACTTTAAGCAAATCGCCAC
CCAGACGTCGCCGATCTGGGACATCGTCAAACGCGGGATGTACGGTATGGCTAACGATCCTAACGGCACCGGATATAAGT
ATTTTCATACGGCGCCCTATCAAATTGCCGGTAAGTCAGGGACCTCACAGGTCTTCGGCCTGAAGCAGAATCAGGTCTAT
AACGCCAGGATGATCCCGGTCAGGCTGCGCGACCATATCTTCTATACGGTATTCGCCCCGTATCCCCACCCGCGCATTGC
GATGGCGGTGATCCTGGAAAACGGCGGCGGGGACGGCGTGACGGCGGCACCGGTCACCCGCGCGATCCTGGACTATATCT
TCCGCCCGGCGGCGGTATCGCCGCCAGCGGCGCCACAGGCGAACGCCCCGCCGGCTCTGCGTCGTGCGGCCTGA

Upstream 100 bases:

>100_bases
GCCGGGCTGCATTTTATGCGTAACCTAACGGTTTAGGTTACTTGATATGCCGTATCGCAGTCGCCCGGCGTCTGCGCATG
CCGCCCGTGTCCCCATTTGC

Downstream 100 bases:

>100_bases
TCGGGTCTACATAAAGAGCCAGAAGAGCAATACCAGCGGAAAGCTCATTGGCCAGGTCAGCCCTACCATAATGGCTGACA
GCAGGCGTATTTTCATGGAG

Product: penicillin-binding protein 2

Products: NA

Alternate protein names: PBP-2 [H]

Number of amino acids: Translated: 637; Mature: 637

Protein sequence:

>637_residues
MNLLKDAIRDHGAESALFLRRTLIALVLVLLCFTVLVVNLYHLQVQEHAAYQTRSDANDIKTLPVSPVRGLIYDRHGTPL
VENQTLYQISLVPGKIPHLDQTLTALTPLLDLTPQEIADFHQAMKHARRFAEVTLKEGLTEREVSRFAVNEFAFPGVMLS
TYQQRYYPYGAQLAHVVGYVSKINDRDLQRLDAEGQRENYAADHDIGKQGIERYYESLLHGKTGSQEVEVDSHGRIVRSL
KEQPPHAGASLYLTLDLPLQQYIEGVLKGQRAAVVVMDPRDGGILAMVSSPSYDPNPFVNGISYKAYSALLHDTALPLIN
RVTQGIYPPASTVKPYMASAALLAGVITPQTTFFGAPTWTLPGTQRRYRDWLKSGHGILNVVKAIEESADTFFYQVAYEM
GIDRIHQWMSQFGYGQLSGIDLDEEARGVLPDRDWKMRVHHKPWYQGDTVSVGIGQGYWTATPIQMVKALGILINNGKVV
TPHLLYAVRRGQQVVPYRQSHFKQIATQTSPIWDIVKRGMYGMANDPNGTGYKYFHTAPYQIAGKSGTSQVFGLKQNQVY
NARMIPVRLRDHIFYTVFAPYPHPRIAMAVILENGGGDGVTAAPVTRAILDYIFRPAAVSPPAAPQANAPPALRRAA

Sequences:

>Translated_637_residues
MNLLKDAIRDHGAESALFLRRTLIALVLVLLCFTVLVVNLYHLQVQEHAAYQTRSDANDIKTLPVSPVRGLIYDRHGTPL
VENQTLYQISLVPGKIPHLDQTLTALTPLLDLTPQEIADFHQAMKHARRFAEVTLKEGLTEREVSRFAVNEFAFPGVMLS
TYQQRYYPYGAQLAHVVGYVSKINDRDLQRLDAEGQRENYAADHDIGKQGIERYYESLLHGKTGSQEVEVDSHGRIVRSL
KEQPPHAGASLYLTLDLPLQQYIEGVLKGQRAAVVVMDPRDGGILAMVSSPSYDPNPFVNGISYKAYSALLHDTALPLIN
RVTQGIYPPASTVKPYMASAALLAGVITPQTTFFGAPTWTLPGTQRRYRDWLKSGHGILNVVKAIEESADTFFYQVAYEM
GIDRIHQWMSQFGYGQLSGIDLDEEARGVLPDRDWKMRVHHKPWYQGDTVSVGIGQGYWTATPIQMVKALGILINNGKVV
TPHLLYAVRRGQQVVPYRQSHFKQIATQTSPIWDIVKRGMYGMANDPNGTGYKYFHTAPYQIAGKSGTSQVFGLKQNQVY
NARMIPVRLRDHIFYTVFAPYPHPRIAMAVILENGGGDGVTAAPVTRAILDYIFRPAAVSPPAAPQANAPPALRRAA
>Mature_637_residues
MNLLKDAIRDHGAESALFLRRTLIALVLVLLCFTVLVVNLYHLQVQEHAAYQTRSDANDIKTLPVSPVRGLIYDRHGTPL
VENQTLYQISLVPGKIPHLDQTLTALTPLLDLTPQEIADFHQAMKHARRFAEVTLKEGLTEREVSRFAVNEFAFPGVMLS
TYQQRYYPYGAQLAHVVGYVSKINDRDLQRLDAEGQRENYAADHDIGKQGIERYYESLLHGKTGSQEVEVDSHGRIVRSL
KEQPPHAGASLYLTLDLPLQQYIEGVLKGQRAAVVVMDPRDGGILAMVSSPSYDPNPFVNGISYKAYSALLHDTALPLIN
RVTQGIYPPASTVKPYMASAALLAGVITPQTTFFGAPTWTLPGTQRRYRDWLKSGHGILNVVKAIEESADTFFYQVAYEM
GIDRIHQWMSQFGYGQLSGIDLDEEARGVLPDRDWKMRVHHKPWYQGDTVSVGIGQGYWTATPIQMVKALGILINNGKVV
TPHLLYAVRRGQQVVPYRQSHFKQIATQTSPIWDIVKRGMYGMANDPNGTGYKYFHTAPYQIAGKSGTSQVFGLKQNQVY
NARMIPVRLRDHIFYTVFAPYPHPRIAMAVILENGGGDGVTAAPVTRAILDYIFRPAAVSPPAAPQANAPPALRRAA

Specific function: Cell wall formation; PBP-2 is responsible for the determination of the rod shape of the cell. Its synthesize cross- linked peptidoglycan from the lipid intermediates [H]

COG id: COG0768

COG function: function code M; Cell division protein FtsI/penicillin-binding protein 2

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786854, Length=611, Percent_Identity=58.7561374795417, Blast_Score=751, Evalue=0.0,
Organism=Escherichia coli, GI1786272, Length=634, Percent_Identity=23.186119873817, Blast_Score=114, Evalue=3e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR005311
- InterPro:   IPR001460
- InterPro:   IPR017790 [H]

Pfam domain/function: PF03717 PBP_dimer; PF00905 Transpeptidase [H]

EC number: NA

Molecular weight: Translated: 70968; Mature: 70968

Theoretical pI: Translated: 9.28; Mature: 9.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLLKDAIRDHGAESALFLRRTLIALVLVLLCFTVLVVNLYHLQVQEHAAYQTRSDANDI
CCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
KTLPVSPVRGLIYDRHGTPLVENQTLYQISLVPGKIPHLDQTLTALTPLLDLTPQEIADF
EECCCCHHHHHEECCCCCCEECCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCHHHHHHH
HQAMKHARRFAEVTLKEGLTEREVSRFAVNEFAFPGVMLSTYQQRYYPYGAQLAHVVGYV
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHH
SKINDRDLQRLDAEGQRENYAADHDIGKQGIERYYESLLHGKTGSQEVEVDSHGRIVRSL
HHCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEECCCHHHHHHH
KEQPPHAGASLYLTLDLPLQQYIEGVLKGQRAAVVVMDPRDGGILAMVSSPSYDPNPFVN
HHCCCCCCCEEEEEECCCHHHHHHHHHCCCCEEEEEECCCCCCEEEEEECCCCCCCCCCC
GISYKAYSALLHDTALPLINRVTQGIYPPASTVKPYMASAALLAGVITPQTTFFGAPTWT
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCEEECCCCCC
LPGTQRRYRDWLKSGHGILNVVKAIEESADTFFYQVAYEMGIDRIHQWMSQFGYGQLSGI
CCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCCC
DLDEEARGVLPDRDWKMRVHHKPWYQGDTVSVGIGQGYWTATPIQMVKALGILINNGKVV
CCCHHHCCCCCCCCCEEEECCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHEEECCCEEE
TPHLLYAVRRGQQVVPYRQSHFKQIATQTSPIWDIVKRGMYGMANDPNGTGYKYFHTAPY
HHHHHHHHHCCCCCCCHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCCCEEEEECCCC
QIAGKSGTSQVFGLKQNQVYNARMIPVRLRDHIFYTVFAPYPHPRIAMAVILENGGGDGV
EEECCCCCCCEEECCCCCEECEEEEEEEEECEEEEEEECCCCCCCEEEEEEEECCCCCCC
TAAPVTRAILDYIFRPAAVSPPAAPQANAPPALRRAA
CHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHCCC
>Mature Secondary Structure
MNLLKDAIRDHGAESALFLRRTLIALVLVLLCFTVLVVNLYHLQVQEHAAYQTRSDANDI
CCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
KTLPVSPVRGLIYDRHGTPLVENQTLYQISLVPGKIPHLDQTLTALTPLLDLTPQEIADF
EECCCCHHHHHEECCCCCCEECCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCHHHHHHH
HQAMKHARRFAEVTLKEGLTEREVSRFAVNEFAFPGVMLSTYQQRYYPYGAQLAHVVGYV
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHH
SKINDRDLQRLDAEGQRENYAADHDIGKQGIERYYESLLHGKTGSQEVEVDSHGRIVRSL
HHCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEECCCHHHHHHH
KEQPPHAGASLYLTLDLPLQQYIEGVLKGQRAAVVVMDPRDGGILAMVSSPSYDPNPFVN
HHCCCCCCCEEEEEECCCHHHHHHHHHCCCCEEEEEECCCCCCEEEEEECCCCCCCCCCC
GISYKAYSALLHDTALPLINRVTQGIYPPASTVKPYMASAALLAGVITPQTTFFGAPTWT
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCEEECCCCCC
LPGTQRRYRDWLKSGHGILNVVKAIEESADTFFYQVAYEMGIDRIHQWMSQFGYGQLSGI
CCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCCC
DLDEEARGVLPDRDWKMRVHHKPWYQGDTVSVGIGQGYWTATPIQMVKALGILINNGKVV
CCCHHHCCCCCCCCCEEEECCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHEEECCCEEE
TPHLLYAVRRGQQVVPYRQSHFKQIATQTSPIWDIVKRGMYGMANDPNGTGYKYFHTAPY
HHHHHHHHHCCCCCCCHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCCCEEEEECCCC
QIAGKSGTSQVFGLKQNQVYNARMIPVRLRDHIFYTVFAPYPHPRIAMAVILENGGGDGV
EEECCCCCCCEEECCCCCEECEEEEEEEEECEEEEEEECCCCCCCEEEEEEEECCCCCCC
TAAPVTRAILDYIFRPAAVSPPAAPQANAPPALRRAA
CHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11677609; 8930920 [H]