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The map label for this gene is mdtK

Identifier: 238919627

GI number: 238919627

Start: 1681666

End: 1683039

Strand: Reverse

Name: mdtK

Synonym: NT01EI_1728

Alternate gene names: 238919627

Gene position: 1683039-1681666 (Counterclockwise)

Preceding gene: 238919629

Following gene: 238919622

Centisome position: 44.15

GC content: 59.68

Gene sequence:

>1374_bases
GTGCAGAAGTATTGGCGCGAAGCGCGTAGTTTATTGGCGTTGGCTATTCCCGTTATCATCGCCCAAATCGCCCAAACGGC
AATGGGCTTTGTCGATACCATCATGGCCGGGAGCGTCAGCGCTACCGATATGGCCGCGGTAGCCGTCGGCACCTCTATCT
GGCTACCGACCATCCTCTTTGGCCACGGCCTGCTGCTGGCCCTGACGCCGGTCATCGCCCAGCTGAATGGCGCAGGTAAA
CGGCCCAGCATACCCCACCAGATCAGTCAGGGATTCTGGCTAGTGGCCGGGCTTTCCGTGCTGATCATCGCTGTCCTGTA
TAATGCCGGGCACATCATCAGCATGATGGACAATATCGATCCGCTGCTGGCCGACAAGGCTATCGGTTATCTGCACGCGA
TTATGTGGGGAGCACCGGGATATCTCTGCTTCCAGGTACTGCGCGGACTATGCGAAGGACTGAGCAAGACCACACCGGGG
ATGGTCATCGGCTTTATCGGTCTACTGATCAACATTCCGGTCAATTATATTTTTATCTACGGTAAATTTGGTGCCCCAGC
GCTGGGCGGCGTTGGCTGCGGCGTCGCCACCGCCAGCGTCTACTGGATCATGTATCTGCTGATGCACAGCTACGTCGCCC
GTGCCCCATCGCTGAAGGATGTCCGTCGACCATGCACATTTGAAGCAGCCGACTGGGCCACGCTCCGCCGCCTAATCGCC
ATCGGAATGCCGATCGCCCTGGCGCTGTTTTTCGAGGTCACCCTGTTCGCCGTGGTCGCCCTGCTGGTCTCTCCGCTGGG
GATCGTGGCGGTCGCCGGGCACCAGGTCGCGCTAAATTTCAGCTCGCTGATGTTTGTGCTGCCCATGTCACTGGGCGTCG
CGGCCACCATCCGCGTCGGCTATAGGCTGGGACAAGGCTCTACGGACAGCGCCCGTGTCTCAGCGCGCACGGCAATCGCG
GTGGGAATGGCGATGGCCACCTGTACCGCCATTTTTACCATCGTCCTGCGCAAACCCATCGCCCTGTTGTATAACGACGA
TGTACAGGTGCTCACCATGGCGACCCACCTGATGCTACTGGCGGCGCTGTACCAAATATCGGACTCCGTGCAGGTGATCG
GCAGTGGCGTTCTGCGTGGCTATAAGGATACCCGCTCGATCTTCTTCATTACCTTTATCGCCTACTGGCTATTGGGCCTG
CCTACCGGGTATCTGCTGGCGCTGACCAACCAGATCACCCCGGCCATGGGGCCGAGCGGCTTCTGGATCGGGTTTATTAT
CGGGCTGACCTCCGCGGCCATCATGATGGCGCTGCGCATCCGCTGGCTACAGCGCCAGCCGAAAAGCTGGATCCTACAGC
GCGCTGCACGCTGA

Upstream 100 bases:

>100_bases
CCCGTTCGTCCCCCTTCTTGTAAGCGACCCACCATGGTGTGCCGTGGGGATAGGCACATCCCTATTTTTTTGTGTCATAA
AAACCATAAAAGGTATATCC

Downstream 100 bases:

>100_bases
ACGCAGAAAAACATCCACTTGAGCGGCGCGGTAGCGCCGCTTTTTTCACCGTACTGCATACTCTGTCAGCAATCGTGTGA
ATTTCTGGGAAAAAAGTGAT

Product: multidrug efflux protein

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Multidrug-efflux transporter

Number of amino acids: Translated: 457; Mature: 457

Protein sequence:

>457_residues
MQKYWREARSLLALAIPVIIAQIAQTAMGFVDTIMAGSVSATDMAAVAVGTSIWLPTILFGHGLLLALTPVIAQLNGAGK
RPSIPHQISQGFWLVAGLSVLIIAVLYNAGHIISMMDNIDPLLADKAIGYLHAIMWGAPGYLCFQVLRGLCEGLSKTTPG
MVIGFIGLLINIPVNYIFIYGKFGAPALGGVGCGVATASVYWIMYLLMHSYVARAPSLKDVRRPCTFEAADWATLRRLIA
IGMPIALALFFEVTLFAVVALLVSPLGIVAVAGHQVALNFSSLMFVLPMSLGVAATIRVGYRLGQGSTDSARVSARTAIA
VGMAMATCTAIFTIVLRKPIALLYNDDVQVLTMATHLMLLAALYQISDSVQVIGSGVLRGYKDTRSIFFITFIAYWLLGL
PTGYLLALTNQITPAMGPSGFWIGFIIGLTSAAIMMALRIRWLQRQPKSWILQRAAR

Sequences:

>Translated_457_residues
MQKYWREARSLLALAIPVIIAQIAQTAMGFVDTIMAGSVSATDMAAVAVGTSIWLPTILFGHGLLLALTPVIAQLNGAGK
RPSIPHQISQGFWLVAGLSVLIIAVLYNAGHIISMMDNIDPLLADKAIGYLHAIMWGAPGYLCFQVLRGLCEGLSKTTPG
MVIGFIGLLINIPVNYIFIYGKFGAPALGGVGCGVATASVYWIMYLLMHSYVARAPSLKDVRRPCTFEAADWATLRRLIA
IGMPIALALFFEVTLFAVVALLVSPLGIVAVAGHQVALNFSSLMFVLPMSLGVAATIRVGYRLGQGSTDSARVSARTAIA
VGMAMATCTAIFTIVLRKPIALLYNDDVQVLTMATHLMLLAALYQISDSVQVIGSGVLRGYKDTRSIFFITFIAYWLLGL
PTGYLLALTNQITPAMGPSGFWIGFIIGLTSAAIMMALRIRWLQRQPKSWILQRAAR
>Mature_457_residues
MQKYWREARSLLALAIPVIIAQIAQTAMGFVDTIMAGSVSATDMAAVAVGTSIWLPTILFGHGLLLALTPVIAQLNGAGK
RPSIPHQISQGFWLVAGLSVLIIAVLYNAGHIISMMDNIDPLLADKAIGYLHAIMWGAPGYLCFQVLRGLCEGLSKTTPG
MVIGFIGLLINIPVNYIFIYGKFGAPALGGVGCGVATASVYWIMYLLMHSYVARAPSLKDVRRPCTFEAADWATLRRLIA
IGMPIALALFFEVTLFAVVALLVSPLGIVAVAGHQVALNFSSLMFVLPMSLGVAATIRVGYRLGQGSTDSARVSARTAIA
VGMAMATCTAIFTIVLRKPIALLYNDDVQVLTMATHLMLLAALYQISDSVQVIGSGVLRGYKDTRSIFFITFIAYWLLGL
PTGYLLALTNQITPAMGPSGFWIGFIIGLTSAAIMMALRIRWLQRQPKSWILQRAAR

Specific function: Multidrug efflux pump that functions probably as a Na(+)/drug antiporter

COG id: COG0534

COG function: function code V; Na+-driven multidrug efflux pump

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family. MdtK subfamily

Homologues:

Organism=Homo sapiens, GI22907060, Length=431, Percent_Identity=22.2737819025522, Blast_Score=90, Evalue=6e-18,
Organism=Homo sapiens, GI153792564, Length=446, Percent_Identity=19.2825112107623, Blast_Score=76, Evalue=8e-14,
Organism=Escherichia coli, GI48994890, Length=457, Percent_Identity=74.1794310722101, Blast_Score=667, Evalue=0.0,
Organism=Escherichia coli, GI226510954, Length=459, Percent_Identity=23.7472766884532, Blast_Score=90, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6321821, Length=389, Percent_Identity=22.107969151671, Blast_Score=74, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6320545, Length=436, Percent_Identity=20.1834862385321, Blast_Score=70, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MDTK_EDWI9 (C5BDY7)

Other databases:

- EMBL:   CP001600
- RefSeq:   YP_002933142.1
- GeneID:   7959043
- GenomeReviews:   CP001600_GR
- KEGG:   eic:NT01EI_1728
- OMA:   WYRRIAR
- ProtClustDB:   PRK01766
- HAMAP:   MF_00400
- InterPro:   IPR002528
- InterPro:   IPR022913
- PANTHER:   PTHR11206
- TIGRFAMs:   TIGR00797

Pfam domain/function: PF01554 MatE

EC number: NA

Molecular weight: Translated: 49124; Mature: 49124

Theoretical pI: Translated: 10.02; Mature: 10.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x1cc7bb94)-; HASH(0x1c746990)-; HASH(0x1cfccf28)-; HASH(0x1c5dcb14)-; HASH(0x1c518490)-; HASH(0x1c776abc)-; HASH(0x1ccd2770)-; HASH(0x1b6e9a68)-; HASH(0x1cac77e8)-; HASH(0x1c2ed1d8)-; HASH(0x40833e98)-; HASH(0x1c797e34)-;

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKYWREARSLLALAIPVIIAQIAQTAMGFVDTIMAGSVSATDMAAVAVGTSIWLPTILF
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
GHGLLLALTPVIAQLNGAGKRPSIPHQISQGFWLVAGLSVLIIAVLYNAGHIISMMDNID
HCHHHHHHHHHHHHHCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHCCCHHHHHHHCCC
PLLADKAIGYLHAIMWGAPGYLCFQVLRGLCEGLSKTTPGMVIGFIGLLINIPVNYIFIY
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCEEEEEEE
GKFGAPALGGVGCGVATASVYWIMYLLMHSYVARAPSLKDVRRPCTFEAADWATLRRLIA
ECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCHHHHHHHHH
IGMPIALALFFEVTLFAVVALLVSPLGIVAVAGHQVALNFSSLMFVLPMSLGVAATIRVG
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHH
YRLGQGSTDSARVSARTAIAVGMAMATCTAIFTIVLRKPIALLYNDDVQVLTMATHLMLL
EEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEECCCHHHHHHHHHHHHH
AALYQISDSVQVIGSGVLRGYKDTRSIFFITFIAYWLLGLPTGYLLALTNQITPAMGPSG
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCC
FWIGFIIGLTSAAIMMALRIRWLQRQPKSWILQRAAR
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCH
>Mature Secondary Structure
MQKYWREARSLLALAIPVIIAQIAQTAMGFVDTIMAGSVSATDMAAVAVGTSIWLPTILF
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
GHGLLLALTPVIAQLNGAGKRPSIPHQISQGFWLVAGLSVLIIAVLYNAGHIISMMDNID
HCHHHHHHHHHHHHHCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHCCCHHHHHHHCCC
PLLADKAIGYLHAIMWGAPGYLCFQVLRGLCEGLSKTTPGMVIGFIGLLINIPVNYIFIY
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCEEEEEEE
GKFGAPALGGVGCGVATASVYWIMYLLMHSYVARAPSLKDVRRPCTFEAADWATLRRLIA
ECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCHHHHHHHHH
IGMPIALALFFEVTLFAVVALLVSPLGIVAVAGHQVALNFSSLMFVLPMSLGVAATIRVG
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHH
YRLGQGSTDSARVSARTAIAVGMAMATCTAIFTIVLRKPIALLYNDDVQVLTMATHLMLL
EEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEECCCHHHHHHHHHHHHH
AALYQISDSVQVIGSGVLRGYKDTRSIFFITFIAYWLLGLPTGYLLALTNQITPAMGPSG
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCC
FWIGFIIGLTSAAIMMALRIRWLQRQPKSWILQRAAR
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA