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The map label for this gene is manX [H]

Identifier: 238919622

GI number: 238919622

Start: 1676246

End: 1677211

Strand: Reverse

Name: manX [H]

Synonym: NT01EI_1721

Alternate gene names: 238919622

Gene position: 1677211-1676246 (Counterclockwise)

Preceding gene: 238919627

Following gene: 238919621

Centisome position: 43.99

GC content: 56.42

Gene sequence:

>966_bases
GTGAGTATTGCTATTATGATCGGTACTCACGGGGCAGCGGCGGAGCAATTGCTAAAAACCGCCGAGATGTTGTTGGGCGA
ACAAAGTAACGTTGGCTTTATCGACTTCGTGCCCGGTGAGAATGCCGAGACCCTGATCGAAAAATACAGTCAGAAGCTGG
CGCAAATGGATATCAGCGAAGGGGTACTCTTCCTGGTGGATACCTGGGGTGGCAGTCCGTTCAACGCGGCCAGCCGGATT
GTATTGGACAAAGAACACTATGACGTGATCACCGGCGTCAACGTACCGATGCTGGTTGAAACCTTCATGGCCCGCGATGA
TAACCCTACGTTTGATGAGCTGGTCGCCTTAGCCTTGGAAACCGGGCGTGAAGGCGTACGCGCACTGCGTGCCAAAGAAG
AGGCCCCCGCGACCAGCGCTCCGAAAGCGGCCACGCCGGTACAGGCTAAACCTGCCACGCCGCTGAACCCGGAGGATCAC
ATGAGGATCGGTCTGGCGCGTATTGACGATCGCCTGATTCATGGTCAGGTTGCCACCCGCTGGACCAAGGAAACCGGCGT
CTCCCGCATCATCGTTGTCAGTGATGAGGTCGCCAACGACCACGTTCGTTCTACCCTGCTTAAGCAGGTGGCGCCGCCGG
GCGTGACCGCCCACGTCGTCGATGTAGCCAAGATGATCCGCGTCTACAATAACCCGAAATACGCCAACGACCGTGTGATG
CTGCTGTTTACCAACCCAACCGACGTCGAGCGTCTGGTGGAGAACGGCGTCAAGATCACCACAGTGAACATCGGCGGTAT
GGCATTCCGGCAGGGGAAAACCCAGGTTAACAATGCCGTCTCCGTCGATGAGACAGATATCGAAGCCTTCAAAAAGCTGG
ATGCCCGTGGCATTGAGCTGGAGGTGCGCAAGGTGTCCAACGATCCGCGTCTGAAGATGATGGACCTTATCAGCAAGATG
AACTGA

Upstream 100 bases:

>100_bases
CATGGATGTTTCGTTAAACAGTTCGTATCTTTAATTGCATACGGCACGTCATGTTTATTTTACGACGTGAAAGTATCAAG
GAAAGCAGAGGAGGCAGCGA

Downstream 100 bases:

>100_bases
TATGCCGTAAGGCATTGATTTAAATAACTAAGATATTTGGTCAAAGGAGAAGTACAATGGAGATTACCACTCTTCAGATT
GTGCTGATATTTATCGTGGC

Product: mannose-specific phosphotransferase system component iiab

Products: NA

Alternate protein names: EIIAB-Man; Mannose-specific phosphotransferase enzyme IIA component; EIII-Man; PTS system mannose-specific EIIA component; Mannose-specific phosphotransferase enzyme IIB component; PTS system mannose-specific EIIB component [H]

Number of amino acids: Translated: 321; Mature: 320

Protein sequence:

>321_residues
MSIAIMIGTHGAAAEQLLKTAEMLLGEQSNVGFIDFVPGENAETLIEKYSQKLAQMDISEGVLFLVDTWGGSPFNAASRI
VLDKEHYDVITGVNVPMLVETFMARDDNPTFDELVALALETGREGVRALRAKEEAPATSAPKAATPVQAKPATPLNPEDH
MRIGLARIDDRLIHGQVATRWTKETGVSRIIVVSDEVANDHVRSTLLKQVAPPGVTAHVVDVAKMIRVYNNPKYANDRVM
LLFTNPTDVERLVENGVKITTVNIGGMAFRQGKTQVNNAVSVDETDIEAFKKLDARGIELEVRKVSNDPRLKMMDLISKM
N

Sequences:

>Translated_321_residues
MSIAIMIGTHGAAAEQLLKTAEMLLGEQSNVGFIDFVPGENAETLIEKYSQKLAQMDISEGVLFLVDTWGGSPFNAASRI
VLDKEHYDVITGVNVPMLVETFMARDDNPTFDELVALALETGREGVRALRAKEEAPATSAPKAATPVQAKPATPLNPEDH
MRIGLARIDDRLIHGQVATRWTKETGVSRIIVVSDEVANDHVRSTLLKQVAPPGVTAHVVDVAKMIRVYNNPKYANDRVM
LLFTNPTDVERLVENGVKITTVNIGGMAFRQGKTQVNNAVSVDETDIEAFKKLDARGIELEVRKVSNDPRLKMMDLISKM
N
>Mature_320_residues
SIAIMIGTHGAAAEQLLKTAEMLLGEQSNVGFIDFVPGENAETLIEKYSQKLAQMDISEGVLFLVDTWGGSPFNAASRIV
LDKEHYDVITGVNVPMLVETFMARDDNPTFDELVALALETGREGVRALRAKEEAPATSAPKAATPVQAKPATPLNPEDHM
RIGLARIDDRLIHGQVATRWTKETGVSRIIVVSDEVANDHVRSTLLKQVAPPGVTAHVVDVAKMIRVYNNPKYANDRVML
LFTNPTDVERLVENGVKITTVNIGGMAFRQGKTQVNNAVSVDETDIEAFKKLDARGIELEVRKVSNDPRLKMMDLISKMN

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG3444

COG function: function code G; Phosphotransferase system, mannose/fructose/N-acetylgalactosamine-specific component IIB

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIB type-4 domain [H]

Homologues:

Organism=Escherichia coli, GI1788120, Length=322, Percent_Identity=81.3664596273292, Blast_Score=520, Evalue=1e-149,
Organism=Escherichia coli, GI87082217, Length=157, Percent_Identity=36.9426751592357, Blast_Score=104, Evalue=8e-24,
Organism=Escherichia coli, GI1789527, Length=159, Percent_Identity=32.7044025157233, Blast_Score=77, Evalue=1e-15,

Paralogues:

None

Copy number: 220 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004701
- InterPro:   IPR013789
- InterPro:   IPR004720
- InterPro:   IPR018455 [H]

Pfam domain/function: PF03610 EIIA-man; PF03830 PTSIIB_sorb [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 35186; Mature: 35055

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: PS51096 PTS_EIIA_TYPE_4 ; PS51101 PTS_EIIB_TYPE_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIAIMIGTHGAAAEQLLKTAEMLLGEQSNVGFIDFVPGENAETLIEKYSQKLAQMDISE
CEEEEEEECCCHHHHHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCCC
GVLFLVDTWGGSPFNAASRIVLDKEHYDVITGVNVPMLVETFMARDDNPTFDELVALALE
CEEEEEECCCCCCCCCCCEEEEECCCCEEEECCCHHHHHHHHHHCCCCCCHHHHHHHHHH
TGREGVRALRAKEEAPATSAPKAATPVQAKPATPLNPEDHMRIGLARIDDRLIHGQVATR
HCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHEEHHHHHCCHHHCCHHHHH
WTKETGVSRIIVVSDEVANDHVRSTLLKQVAPPGVTAHVVDVAKMIRVYNNPKYANDRVM
HHHHCCCEEEEEEECHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCEEE
LLFTNPTDVERLVENGVKITTVNIGGMAFRQGKTQVNNAVSVDETDIEAFKKLDARGIEL
EEECCCHHHHHHHHCCCEEEEEEECCEEEECCCHHHCCCCCCCHHHHHHHHHHCCCCCEE
EVRKVSNDPRLKMMDLISKMN
EEEECCCCCCHHHHHHHHHCC
>Mature Secondary Structure 
SIAIMIGTHGAAAEQLLKTAEMLLGEQSNVGFIDFVPGENAETLIEKYSQKLAQMDISE
EEEEEEECCCHHHHHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCCC
GVLFLVDTWGGSPFNAASRIVLDKEHYDVITGVNVPMLVETFMARDDNPTFDELVALALE
CEEEEEECCCCCCCCCCCEEEEECCCCEEEECCCHHHHHHHHHHCCCCCCHHHHHHHHHH
TGREGVRALRAKEEAPATSAPKAATPVQAKPATPLNPEDHMRIGLARIDDRLIHGQVATR
HCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHEEHHHHHCCHHHCCHHHHH
WTKETGVSRIIVVSDEVANDHVRSTLLKQVAPPGVTAHVVDVAKMIRVYNNPKYANDRVM
HHHHCCCEEEEEEECHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCEEE
LLFTNPTDVERLVENGVKITTVNIGGMAFRQGKTQVNNAVSVDETDIEAFKKLDARGIEL
EEECCCHHHHHHHHCCCEEEEEEECCEEEECCCHHHCCCCCCCHHHHHHHHHHCCCCCEE
EVRKVSNDPRLKMMDLISKMN
EEEECCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]