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The map label for this gene is purU [H]

Identifier: 238919560

GI number: 238919560

Start: 1617445

End: 1618293

Strand: Reverse

Name: purU [H]

Synonym: NT01EI_1659

Alternate gene names: 238919560

Gene position: 1618293-1617445 (Counterclockwise)

Preceding gene: 238919561

Following gene: 238919558

Centisome position: 42.45

GC content: 55.12

Gene sequence:

>849_bases
ATGTCACAACCCGTTATTCAACGAAAAGTCCTGCGTACCGTCTGCCCCGATGCCAAGGGGCTGATCGCCAAAATTACCAA
TATCTGTTACAAGCATCAGCTCAATATTATTCAGAACAATGAGTTCGTCGACCACGCCAGCGGGCGCTTCTTTATGCGTA
CCGAACTTGAGGGGAGCTTTAACGATGCCACCCTGCTGGCCGATCTGGATGACGCACTGCCGCCGGGCTCCGCTCGCGAA
ATCATCACCACATCCCGTCAGCGCATAGTAATCCTGGTCACAAAAGAGGCCCACTGCCTGGGCGATCTGCTGATCAAGAG
CGCCTATGGCGGACTGGACGTCGAGATTGCCGCCGTCATCGGCAACCACGCGACGCTACAGGCGTTAGTCGAAAAGTTTG
ATATCCCCTTCGTCCTGATCGGTCACGAAGGCCTCAGCCGTGAGGCGCACGATGCGGCCGTTGCCGAACAGATCGATCGC
TTTGAACCGGACTATGTGGTACTGGCCAAATATATGCGGGTGCTGACCCCCGGCTTTGTTGCCCGCTACCCTGACCGCAT
CATCAATATTCATCACTCGTTTCTTCCTGCGTTTATCGGCGCCCGTCCCTACCACCAGGCCTATCAGCGAGGAGTGAAGA
TCATTGGTGCGACGGCGCACTTTGTAAACAATGATTTGGATGAAGGCCCGATCATCATGCAGGACGTTATTCACGTCGAT
CACAGCTATACCGCAGAGGAGATGATACGCGCGGGGCGCGATGTGGAAAAAAATGTGCTCAGCCGGGCCCTGTATCGCGT
ACTGGCTCAGCGGGTGTTCGTCTGTGGCAACCGCACTGTAATTTTCTGA

Upstream 100 bases:

>100_bases
TGCGGACGCTAGGCTGCGTCCCGCCCTGAACGGGGGAATTGACACTCCCTTCTCGGTGGACGTCGTGATAACGGCCCGGC
AAGAACAAGGATGCCAACAC

Downstream 100 bases:

>100_bases
TTTGTTCATTTTTTCTCTGTTTTCTGTACGCCCGCACAGCAGTGCGGGCAAAAAAACGGCAAACAATTTTTTTTTCCTCT
CCAGCGCTTTACAGCGCGGT

Product: formyltetrahydrofolate deformylase

Products: NA

Alternate protein names: Formyl-FH(4) hydrolase [H]

Number of amino acids: Translated: 282; Mature: 281

Protein sequence:

>282_residues
MSQPVIQRKVLRTVCPDAKGLIAKITNICYKHQLNIIQNNEFVDHASGRFFMRTELEGSFNDATLLADLDDALPPGSARE
IITTSRQRIVILVTKEAHCLGDLLIKSAYGGLDVEIAAVIGNHATLQALVEKFDIPFVLIGHEGLSREAHDAAVAEQIDR
FEPDYVVLAKYMRVLTPGFVARYPDRIINIHHSFLPAFIGARPYHQAYQRGVKIIGATAHFVNNDLDEGPIIMQDVIHVD
HSYTAEEMIRAGRDVEKNVLSRALYRVLAQRVFVCGNRTVIF

Sequences:

>Translated_282_residues
MSQPVIQRKVLRTVCPDAKGLIAKITNICYKHQLNIIQNNEFVDHASGRFFMRTELEGSFNDATLLADLDDALPPGSARE
IITTSRQRIVILVTKEAHCLGDLLIKSAYGGLDVEIAAVIGNHATLQALVEKFDIPFVLIGHEGLSREAHDAAVAEQIDR
FEPDYVVLAKYMRVLTPGFVARYPDRIINIHHSFLPAFIGARPYHQAYQRGVKIIGATAHFVNNDLDEGPIIMQDVIHVD
HSYTAEEMIRAGRDVEKNVLSRALYRVLAQRVFVCGNRTVIF
>Mature_281_residues
SQPVIQRKVLRTVCPDAKGLIAKITNICYKHQLNIIQNNEFVDHASGRFFMRTELEGSFNDATLLADLDDALPPGSAREI
ITTSRQRIVILVTKEAHCLGDLLIKSAYGGLDVEIAAVIGNHATLQALVEKFDIPFVLIGHEGLSREAHDAAVAEQIDRF
EPDYVVLAKYMRVLTPGFVARYPDRIINIHHSFLPAFIGARPYHQAYQRGVKIIGATAHFVNNDLDEGPIIMQDVIHVDH
SYTAEEMIRAGRDVEKNVLSRALYRVLAQRVFVCGNRTVIF

Specific function: Produces formate from formyl-tetrahydrofolate. Provides the major source of formate for the purT-dependent synthesis of 5'-phosphoribosyl-N-formylglycinamide (FGAR) during aerobic growth. Has a role in regulating the one-carbon pool [H]

COG id: COG0788

COG function: function code F; Formyltetrahydrofolate hydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ACT domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=200, Percent_Identity=29.5, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI209869995, Length=200, Percent_Identity=29.5, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI209869993, Length=200, Percent_Identity=29.5, Blast_Score=94, Evalue=1e-19,
Organism=Escherichia coli, GI1787483, Length=277, Percent_Identity=80.14440433213, Blast_Score=475, Evalue=1e-135,
Organism=Escherichia coli, GI1788846, Length=89, Percent_Identity=46.0674157303371, Blast_Score=91, Evalue=8e-20,
Organism=Caenorhabditis elegans, GI17567511, Length=196, Percent_Identity=32.6530612244898, Blast_Score=101, Evalue=5e-22,
Organism=Drosophila melanogaster, GI24582400, Length=166, Percent_Identity=30.1204819277108, Blast_Score=84, Evalue=7e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR002376
- InterPro:   IPR004810 [H]

Pfam domain/function: PF01842 ACT; PF00551 Formyl_trans_N [H]

EC number: =3.5.1.10 [H]

Molecular weight: Translated: 31555; Mature: 31424

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQPVIQRKVLRTVCPDAKGLIAKITNICYKHQLNIIQNNEFVDHASGRFFMRTELEGSF
CCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCEEECCCEEECCCCCEEEEEECCCCC
NDATLLADLDDALPPGSAREIITTSRQRIVILVTKEAHCLGDLLIKSAYGGLDVEIAAVI
CCEEEEEECHHCCCCCCCHHHHHCCCCEEEEEEECCHHHHHHHHHHHHCCCCCEEEEEEC
GNHATLQALVEKFDIPFVLIGHEGLSREAHDAAVAEQIDRFEPDYVVLAKYMRVLTPGFV
CCHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHH
ARYPDRIINIHHSFLPAFIGARPYHQAYQRGVKIIGATAHFVNNDLDEGPIIMQDVIHVD
HHCCHHHHHHHHHHHHHHHCCCCHHHHHHCCCEEEEHHHHHHCCCCCCCCHHHHHHHHCC
HSYTAEEMIRAGRDVEKNVLSRALYRVLAQRVFVCGNRTVIF
CCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEC
>Mature Secondary Structure 
SQPVIQRKVLRTVCPDAKGLIAKITNICYKHQLNIIQNNEFVDHASGRFFMRTELEGSF
CCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCEEECCCEEECCCCCEEEEEECCCCC
NDATLLADLDDALPPGSAREIITTSRQRIVILVTKEAHCLGDLLIKSAYGGLDVEIAAVI
CCEEEEEECHHCCCCCCCHHHHHCCCCEEEEEEECCHHHHHHHHHHHHCCCCCEEEEEEC
GNHATLQALVEKFDIPFVLIGHEGLSREAHDAAVAEQIDRFEPDYVVLAKYMRVLTPGFV
CCHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHH
ARYPDRIINIHHSFLPAFIGARPYHQAYQRGVKIIGATAHFVNNDLDEGPIIMQDVIHVD
HHCCHHHHHHHHHHHHHHHCCCCHHHHHHCCCEEEEHHHHHHCCCCCCCCHHHHHHHHCC
HSYTAEEMIRAGRDVEKNVLSRALYRVLAQRVFVCGNRTVIF
CCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]