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The map label for this gene is kdsA

Identifier: 238919473

GI number: 238919473

Start: 1528560

End: 1529414

Strand: Direct

Name: kdsA

Synonym: NT01EI_1567

Alternate gene names: 238919473

Gene position: 1528560-1529414 (Clockwise)

Preceding gene: 238919472

Following gene: 238919478

Centisome position: 40.1

GC content: 59.53

Gene sequence:

>855_bases
ATGAAACAGAAAGTTGTCAGCATTGGTGATATTCAGGTCGCAAACGATCTGCCGTTTGTTCTGTTTGGTGGGATGAACGT
GCTGGAGTCGCGCGATCTGGCGATGCGCATCTGTGAGCACTACGTTACCGTGACCCAAAAGCTGGGTATCCCCTATGTCT
TTAAGGCCTCCTTCGACAAGGCCAACCGCTCCTCTATCCACTCGTACCGCGGTCCGGGCCTGGAAGAGGGGATGAAGATT
TTCCAGGAGCTGAAGCGAACCTTCGGGGTGAAGATCATCACCGATGTCCATACGCCGGAGCAGGCGCAGCCGGTGGCTGA
CGTGGTGGATATCATTCAGCTGCCCGCCTTCCTGGCGCGCCAGACCGATCTGGTTGAGGCGATGGCGAAAACCGGCGCAG
TGATCAACGTTAAGAAGCCGCAGTTTTTAAGCCCGGGCCAGATGGGCAATATCGTGGACAAGTTCCGGGAGGGGGGCAAC
GATCGGGTGATCCTGTGCGATCGCGGCAGCAACTTTGGCTATGACAATCTGGTGGTCGACATGCTGGGCTTTGGGGTGAT
GAAGAGTGTCAGCCACGGGGCACCGGTGATTTTTGACGTGACCCACTCCCTGCAGTGCCGCGATCCGTTTGGCGCCGCCT
CCGGTGGCCGTCGTGCCCAGGTGACGGAGCTGGCGCGTTCCGGGATGGCCATCGGCCTGGCCGGACTGTTTATTGAAGCG
CACCCGGATCCGGTCCACGCCATGTGTGACGGCCCCTCCGCGCTGCCGCTGGAGAAGCTGGAGCCGTTCCTGGTCCAGAT
GAAGGCCATCGACGATCTGGTGAAGAACTTCCCGCCGCTGGATACCGCCAACTAA

Upstream 100 bases:

>100_bases
TTTTGACTGCGCAGGAGCGGCGACAGACGGTGGAGTATGGCATACTCTGCGCGTTCGCTGTTCGGTCAGCAGGTTTTTTT
TCAAAATTTAAGGCGCAAGC

Downstream 100 bases:

>100_bases
GGCGTGGGCCGTACGGCCCACCTATATATATCGGGGGAGCCCAGGCTCTCCCGTTTTTTTTATCCGCGCTGGCAGAATAT
CCCCTGTACGCGCCCATCGG

Product: 2-dehydro-3-deoxyphosphooctonate aldolase

Products: NA

Alternate protein names: 3-deoxy-D-manno-octulosonic acid 8-phosphate synthase; KDO-8-phosphate synthase; KDO 8-P synthase; KDOPS; Phospho-2-dehydro-3-deoxyoctonate aldolase

Number of amino acids: Translated: 284; Mature: 284

Protein sequence:

>284_residues
MKQKVVSIGDIQVANDLPFVLFGGMNVLESRDLAMRICEHYVTVTQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKI
FQELKRTFGVKIITDVHTPEQAQPVADVVDIIQLPAFLARQTDLVEAMAKTGAVINVKKPQFLSPGQMGNIVDKFREGGN
DRVILCDRGSNFGYDNLVVDMLGFGVMKSVSHGAPVIFDVTHSLQCRDPFGAASGGRRAQVTELARSGMAIGLAGLFIEA
HPDPVHAMCDGPSALPLEKLEPFLVQMKAIDDLVKNFPPLDTAN

Sequences:

>Translated_284_residues
MKQKVVSIGDIQVANDLPFVLFGGMNVLESRDLAMRICEHYVTVTQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKI
FQELKRTFGVKIITDVHTPEQAQPVADVVDIIQLPAFLARQTDLVEAMAKTGAVINVKKPQFLSPGQMGNIVDKFREGGN
DRVILCDRGSNFGYDNLVVDMLGFGVMKSVSHGAPVIFDVTHSLQCRDPFGAASGGRRAQVTELARSGMAIGLAGLFIEA
HPDPVHAMCDGPSALPLEKLEPFLVQMKAIDDLVKNFPPLDTAN
>Mature_284_residues
MKQKVVSIGDIQVANDLPFVLFGGMNVLESRDLAMRICEHYVTVTQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKI
FQELKRTFGVKIITDVHTPEQAQPVADVVDIIQLPAFLARQTDLVEAMAKTGAVINVKKPQFLSPGQMGNIVDKFREGGN
DRVILCDRGSNFGYDNLVVDMLGFGVMKSVSHGAPVIFDVTHSLQCRDPFGAASGGRRAQVTELARSGMAIGLAGLFIEA
HPDPVHAMCDGPSALPLEKLEPFLVQMKAIDDLVKNFPPLDTAN

Specific function: Synthesis Of Kdo 8-P Which Is Required For Lipid A Maturation And Cellular Growth. [C]

COG id: COG2877

COG function: function code M; 3-deoxy-D-manno-octulosonic acid (KDO) 8-phosphate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the kdsA family

Homologues:

Organism=Escherichia coli, GI1787466, Length=284, Percent_Identity=89.7887323943662, Blast_Score=530, Evalue=1e-152,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): KDSA_EDWI9 (C5B817)

Other databases:

- EMBL:   CP001600
- RefSeq:   YP_002932988.1
- GeneID:   7961048
- GenomeReviews:   CP001600_GR
- KEGG:   eic:NT01EI_1567
- OMA:   QLILCDR
- ProtClustDB:   PRK05198
- GO:   GO:0005737
- HAMAP:   MF_00056
- InterPro:   IPR013785
- InterPro:   IPR006218
- InterPro:   IPR006269
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR21057:SF2
- TIGRFAMs:   TIGR01362

Pfam domain/function: PF00793 DAHP_synth_1

EC number: =2.5.1.55

Molecular weight: Translated: 30938; Mature: 30938

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQKVVSIGDIQVANDLPFVLFGGMNVLESRDLAMRICEHYVTVTQKLGIPYVFKASFDK
CCCCEEEECCEEECCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCC
ANRSSIHSYRGPGLEEGMKIFQELKRTFGVKIITDVHTPEQAQPVADVVDIIQLPAFLAR
CCHHHHHHHCCCCHHHHHHHHHHHHHHHCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHH
QTDLVEAMAKTGAVINVKKPQFLSPGQMGNIVDKFREGGNDRVILCDRGSNFGYDNLVVD
HHHHHHHHHHCCCEEEECCCCCCCCCCCHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHH
MLGFGVMKSVSHGAPVIFDVTHSLQCRDPFGAASGGRRAQVTELARSGMAIGLAGLFIEA
HHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHCCCEEEEEEEEEEC
HPDPVHAMCDGPSALPLEKLEPFLVQMKAIDDLVKNFPPLDTAN
CCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MKQKVVSIGDIQVANDLPFVLFGGMNVLESRDLAMRICEHYVTVTQKLGIPYVFKASFDK
CCCCEEEECCEEECCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCC
ANRSSIHSYRGPGLEEGMKIFQELKRTFGVKIITDVHTPEQAQPVADVVDIIQLPAFLAR
CCHHHHHHHCCCCHHHHHHHHHHHHHHHCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHH
QTDLVEAMAKTGAVINVKKPQFLSPGQMGNIVDKFREGGNDRVILCDRGSNFGYDNLVVD
HHHHHHHHHHCCCEEEECCCCCCCCCCCHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHH
MLGFGVMKSVSHGAPVIFDVTHSLQCRDPFGAASGGRRAQVTELARSGMAIGLAGLFIEA
HHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHCCCEEEEEEEEEEC
HPDPVHAMCDGPSALPLEKLEPFLVQMKAIDDLVKNFPPLDTAN
CCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA