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The map label for this gene is hemK [H]

Identifier: 238919471

GI number: 238919471

Start: 1526684

End: 1527523

Strand: Direct

Name: hemK [H]

Synonym: NT01EI_1565

Alternate gene names: 238919471

Gene position: 1526684-1527523 (Clockwise)

Preceding gene: 238919470

Following gene: 238919472

Centisome position: 40.05

GC content: 66.67

Gene sequence:

>840_bases
ATGCGCTACGATCAATGGTTGCGTCAGGCCTGCGCGCGCCTGACGCCGGGCGACAGCCCACGGCGCGATGCGGAGATCCT
GCTGGAGCATGTGACCGGCAGGGGGCGCAGCTTTCTGCTGGCCTTTGGCGAGACGCTGTTGACGGCGGCACAGCTGGCGC
AGCTGACCTCTCTGCTGGCGCGGCGCGTGCAGGGAGAGCCGGTGGCTTATCTGATCGGCGAACGTGAGTTTTGGTCGCTG
CCGCTGGCGGTTTCTCCGGCGACGCTGATCCCGCGTCCGGATACCGAATGTCTGGTCGAACAGGCGCTGCTGCGTCTGCC
TGCGACACCGGTGCAGATTGTCGATCTGGGCACCGGCACCGGTGCCATCGCTCTGGCGCTGGCCAGTGAGCGCCCCGACT
GCCAGGTGAGTGCGGTGGAGTTCAACCCCGATGCCGTGGCGCTGGCGCAGCATAACGCCGCCCGTCTGGGCCTGTCGCGG
GTCGAGATCCTGCAGGGCAGCTGGTTTACGCCGCTGGCCGGGCGGCGTTTTACCCTGATCGTTTCTAACCCGCCCTATAT
CGATGCAGCCGATGGCCACCTGAGCCAGGGCGACGTGCGCTTTGAGCCCGCCAGTGCGCTGGTGGCTGCCGAGCAGGGAC
TGGCCGATCTGCGGGCGATTGCCCGTCAGGCGCCGGACTATCTGGCGCTGGGGGGATGGTTGTTGCTGGAGCACGGCTGG
CAGCAGGGGGCTGCCGTGCGCGCCCTGTTGACAGAGTATGGCTTTTGTCGCGTGGAGAGCGTGCGGGACTACGGCAATAA
CGAACGGGTGACCTTAGGACAGTACTGTCCGTCGTGCTGA

Upstream 100 bases:

>100_bases
GTCTGGATGAGGTGATGGAGGGGAAGCTGGACAACCTGATTGAGCCCATCGTGCAGGAGCACCAGGCCGATCAGCTCAGC
GCGCTCGCCGAGCAGGAGTG

Downstream 100 bases:

>100_bases
GGCGGCGGGATAGGCCGGATATGATGATGAAACAGACGATCAACGGGCTGCCGCTGATGATGGAATGGGTATGAGTGTAA
TAGCCGATTTTGCCTTTGAT

Product: protein-(glutamine-N5) methyltransferase, release factor-specific

Products: NA

Alternate protein names: M.EcoKHemKP; Protein-(glutamine-N(5)) MTase hemK; Protein-glutamine N-methyltransferase hemK [H]

Number of amino acids: Translated: 279; Mature: 279

Protein sequence:

>279_residues
MRYDQWLRQACARLTPGDSPRRDAEILLEHVTGRGRSFLLAFGETLLTAAQLAQLTSLLARRVQGEPVAYLIGEREFWSL
PLAVSPATLIPRPDTECLVEQALLRLPATPVQIVDLGTGTGAIALALASERPDCQVSAVEFNPDAVALAQHNAARLGLSR
VEILQGSWFTPLAGRRFTLIVSNPPYIDAADGHLSQGDVRFEPASALVAAEQGLADLRAIARQAPDYLALGGWLLLEHGW
QQGAAVRALLTEYGFCRVESVRDYGNNERVTLGQYCPSC

Sequences:

>Translated_279_residues
MRYDQWLRQACARLTPGDSPRRDAEILLEHVTGRGRSFLLAFGETLLTAAQLAQLTSLLARRVQGEPVAYLIGEREFWSL
PLAVSPATLIPRPDTECLVEQALLRLPATPVQIVDLGTGTGAIALALASERPDCQVSAVEFNPDAVALAQHNAARLGLSR
VEILQGSWFTPLAGRRFTLIVSNPPYIDAADGHLSQGDVRFEPASALVAAEQGLADLRAIARQAPDYLALGGWLLLEHGW
QQGAAVRALLTEYGFCRVESVRDYGNNERVTLGQYCPSC
>Mature_279_residues
MRYDQWLRQACARLTPGDSPRRDAEILLEHVTGRGRSFLLAFGETLLTAAQLAQLTSLLARRVQGEPVAYLIGEREFWSL
PLAVSPATLIPRPDTECLVEQALLRLPATPVQIVDLGTGTGAIALALASERPDCQVSAVEFNPDAVALAQHNAARLGLSR
VEILQGSWFTPLAGRRFTLIVSNPPYIDAADGHLSQGDVRFEPASALVAAEQGLADLRAIARQAPDYLALGGWLLLEHGW
QQGAAVRALLTEYGFCRVESVRDYGNNERVTLGQYCPSC

Specific function: Methylates the translation termination release factor RF1/PrfA on 'Gln-235' and RF2/PrfB on 'Gln-252' [H]

COG id: COG2890

COG function: function code J; Methylase of polypeptide chain release factors

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hemK family [H]

Homologues:

Organism=Homo sapiens, GI7705409, Length=236, Percent_Identity=35.5932203389831, Blast_Score=95, Evalue=6e-20,
Organism=Escherichia coli, GI1787463, Length=275, Percent_Identity=66.1818181818182, Blast_Score=370, Evalue=1e-104,
Organism=Escherichia coli, GI87082085, Length=196, Percent_Identity=35.7142857142857, Blast_Score=103, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6324266, Length=173, Percent_Identity=28.3236994219653, Blast_Score=69, Evalue=7e-13,
Organism=Drosophila melanogaster, GI24582226, Length=235, Percent_Identity=28.936170212766, Blast_Score=95, Evalue=4e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004556
- InterPro:   IPR019874
- InterPro:   IPR007848 [H]

Pfam domain/function: PF05175 MTS [H]

EC number: 2.1.1.-

Molecular weight: Translated: 30312; Mature: 30312

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRYDQWLRQACARLTPGDSPRRDAEILLEHVTGRGRSFLLAFGETLLTAAQLAQLTSLLA
CCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHH
RRVQGEPVAYLIGEREFWSLPLAVSPATLIPRPDTECLVEQALLRLPATPVQIVDLGTGT
HHHCCCCEEEEECCCHHEECCEEECCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCC
GAIALALASERPDCQVSAVEFNPDAVALAQHNAARLGLSRVEILQGSWFTPLAGRRFTLI
CEEEEEEECCCCCCEEEEEEECCCEEEEECCCHHHHCHHHHHHHCCCCCCCCCCCEEEEE
VSNPPYIDAADGHLSQGDVRFEPASALVAAEQGLADLRAIARQAPDYLALGGWLLLEHGW
ECCCCEEECCCCCCCCCCEEECCHHHHHHHHHCHHHHHHHHHHCCCCEECCCCCEECCCC
QQGAAVRALLTEYGFCRVESVRDYGNNERVTLGQYCPSC
CCHHHHHHHHHHCCCEEEEHHHHCCCCCCEEECCCCCCC
>Mature Secondary Structure
MRYDQWLRQACARLTPGDSPRRDAEILLEHVTGRGRSFLLAFGETLLTAAQLAQLTSLLA
CCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHH
RRVQGEPVAYLIGEREFWSLPLAVSPATLIPRPDTECLVEQALLRLPATPVQIVDLGTGT
HHHCCCCEEEEECCCHHEECCEEECCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCC
GAIALALASERPDCQVSAVEFNPDAVALAQHNAARLGLSRVEILQGSWFTPLAGRRFTLI
CEEEEEEECCCCCCEEEEEEECCCEEEEECCCHHHHCHHHHHHHCCCCCCCCCCCEEEEE
VSNPPYIDAADGHLSQGDVRFEPASALVAAEQGLADLRAIARQAPDYLALGGWLLLEHGW
ECCCCEEECCCCCCCCCCEEECCHHHHHHHHHCHHHHHHHHHHCCCCEECCCCCEECCCC
QQGAAVRALLTEYGFCRVESVRDYGNNERVTLGQYCPSC
CCHHHHHHHHHHCCCEEEEHHHHCCCCCCEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7883187; 7543480; 11805295; 8905232; 9278503 [H]