Click here to switch to the map view.

The map label for this gene is arnB

Identifier: 238919322

GI number: 238919322

Start: 1400067

End: 1401212

Strand: Direct

Name: arnB

Synonym: NT01EI_1413

Alternate gene names: 238919322

Gene position: 1400067-1401212 (Clockwise)

Preceding gene: 238919313

Following gene: 238919323

Centisome position: 36.72

GC content: 60.38

Gene sequence:

>1146_bases
ATGAAGGACTTTTTGCCATTCTCCAAGCCGGCTATCGGCGATGAGGAGATTGCTGCTGTCAGTGAAGTACTGCGCTCCGG
CTGGATTACGACCGGTCCGCAAAATCAGGCGTTGGAGCAAGAGTTCTGTCAGGCCACCGGCGCCCGTCATGCTATCGCCG
TGTGCTCAGCGACGGCGGGAATGCACGTTACCCTGATGGCTCTGGGCATTGGGCCGGGGGATGAGGTAATCACCCCCTCA
ATGACCTGGGTTTCCACCCTGAATATCATCACGCTGCTGGGGGCGACCCCCGTGATGATCGATGTCGGGCGCGATACGCT
GATGGTGACACCAGAGGCGATTGCCGATGCGATAACCCCCCGCACCAAGGCGATTATTCCGGTTCATTTTGCCGGTGCCC
CCGTCGATCTTGACCCGATCCGCGCGCTGGCGCAGCGGCAGGGTATCGCCCTGATCGAAGATGCGGCCCACGCCATTGGC
ACCGCCTATCGCGGTGAGCCTATCGGTCAGCGCGGCACCGCTATTTTTTCGTTTCATGCGATTAAAAATGTCACCTGTGC
CGAGGGTGGCATGGTGGTGACCGATGATGATCGCCTGGCGCAGCAGGTGCGCAGCCTTAAGTTTCACGGTCTGGGCGTCG
ATGCCTACGATCGCCAGACTCATGGCCGGGCGCCGCAGGCGGAGGTGGTAACGCCGGGATACAAGTACAACCTGGCCGAT
ATCAATGCGGCGATTGCCCGGGTACAGCTGGCAAAGCTGCCTGAGCTCAATGCCCGACGCGCCCGGCTGGCGGCCTATTA
TCTGCAACAGCTGGCGCAGCATCAGCTGCCGTTCTCCCCGCTGAACACACCGGAGTGGCCACATCAGCATGCCTGGCACC
TGTTTATCATCCGCTGTGATGAGGCCCGCAGCGGCGTGGATCGCGATCGGCTGATGCAGGCGCTCAAAGAGCGCAATATC
GGCAGCGGGTTGCACTTCCGTGCCGCCCATACGCAAAAATATTACCGCGAGAGCTACCCACAGCTGCGGCTGGCGCAGAC
GGAGTGGAACTCACAGCGAATCTGCTCTCTGCCGCTGTTTCCGGATATGACCGAGGCAGACGTCGATCGGGTGATCGCCG
CGTTGAAAGAGATTATTGAGCAGTAA

Upstream 100 bases:

>100_bases
TCTTGGCTTATCGTCCTGCGAGGGAAATAGCGCCATGGATTGTTGTAATTTCGATATGGATCACATTTTTCATTTCTATT
ACTGATATGGGAACGTGTTA

Downstream 100 bases:

>100_bases
CGGTTGGATATGACAATGAGTGATTTTAAAACCATCCAGTTTGTATCGGTGGTAATACCGGTTTACAACGAGCAGGACAG
CCTGTCGGAGCTGCTGAAAA

Product: UDP-4-amino-4-deoxy-L-arabinose--oxoglutarate aminotransferase

Products: NA

Alternate protein names: UDP-(beta-L-threo-pentapyranosyl-4''-ulose diphosphate) aminotransferase; UDP-Ara4O aminotransferase; UDP-4-amino-4-deoxy-L-arabinose aminotransferase

Number of amino acids: Translated: 381; Mature: 381

Protein sequence:

>381_residues
MKDFLPFSKPAIGDEEIAAVSEVLRSGWITTGPQNQALEQEFCQATGARHAIAVCSATAGMHVTLMALGIGPGDEVITPS
MTWVSTLNIITLLGATPVMIDVGRDTLMVTPEAIADAITPRTKAIIPVHFAGAPVDLDPIRALAQRQGIALIEDAAHAIG
TAYRGEPIGQRGTAIFSFHAIKNVTCAEGGMVVTDDDRLAQQVRSLKFHGLGVDAYDRQTHGRAPQAEVVTPGYKYNLAD
INAAIARVQLAKLPELNARRARLAAYYLQQLAQHQLPFSPLNTPEWPHQHAWHLFIIRCDEARSGVDRDRLMQALKERNI
GSGLHFRAAHTQKYYRESYPQLRLAQTEWNSQRICSLPLFPDMTEADVDRVIAALKEIIEQ

Sequences:

>Translated_381_residues
MKDFLPFSKPAIGDEEIAAVSEVLRSGWITTGPQNQALEQEFCQATGARHAIAVCSATAGMHVTLMALGIGPGDEVITPS
MTWVSTLNIITLLGATPVMIDVGRDTLMVTPEAIADAITPRTKAIIPVHFAGAPVDLDPIRALAQRQGIALIEDAAHAIG
TAYRGEPIGQRGTAIFSFHAIKNVTCAEGGMVVTDDDRLAQQVRSLKFHGLGVDAYDRQTHGRAPQAEVVTPGYKYNLAD
INAAIARVQLAKLPELNARRARLAAYYLQQLAQHQLPFSPLNTPEWPHQHAWHLFIIRCDEARSGVDRDRLMQALKERNI
GSGLHFRAAHTQKYYRESYPQLRLAQTEWNSQRICSLPLFPDMTEADVDRVIAALKEIIEQ
>Mature_381_residues
MKDFLPFSKPAIGDEEIAAVSEVLRSGWITTGPQNQALEQEFCQATGARHAIAVCSATAGMHVTLMALGIGPGDEVITPS
MTWVSTLNIITLLGATPVMIDVGRDTLMVTPEAIADAITPRTKAIIPVHFAGAPVDLDPIRALAQRQGIALIEDAAHAIG
TAYRGEPIGQRGTAIFSFHAIKNVTCAEGGMVVTDDDRLAQQVRSLKFHGLGVDAYDRQTHGRAPQAEVVTPGYKYNLAD
INAAIARVQLAKLPELNARRARLAAYYLQQLAQHQLPFSPLNTPEWPHQHAWHLFIIRCDEARSGVDRDRLMQALKERNI
GSGLHFRAAHTQKYYRESYPQLRLAQTEWNSQRICSLPLFPDMTEADVDRVIAALKEIIEQ

Specific function: Catalyzes the conversion of UDP-4-keto-arabinose (UDP- Ara4O) to UDP-4-amino-4-deoxy-L-arabinose (UDP-L-Ara4N). The modified arabinose is attached to lipid A and is required for resistance to polymyxin and cationic antimicrobial peptides

COG id: COG0399

COG function: function code M; Predicted pyridoxal phosphate-dependent enzyme apparently involved in regulation of cell wall biogenesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the degT/dnrJ/eryC1 family. ArnB subfamily

Homologues:

Organism=Escherichia coli, GI145693159, Length=381, Percent_Identity=73.753280839895, Blast_Score=582, Evalue=1e-167,
Organism=Escherichia coli, GI2367285, Length=387, Percent_Identity=31.5245478036176, Blast_Score=158, Evalue=6e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ARNB_EDWI9 (C5BDQ4)

Other databases:

- EMBL:   CP001600
- RefSeq:   YP_002932837.1
- GeneID:   7962229
- GenomeReviews:   CP001600_GR
- KEGG:   eic:NT01EI_1413
- OMA:   NVTCAEG
- ProtClustDB:   PRK11658
- HAMAP:   MF_01167
- InterPro:   IPR022850
- InterPro:   IPR000653
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PIRSF:   PIRSF000390

Pfam domain/function: PF01041 DegT_DnrJ_EryC1; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =2.6.1.87

Molecular weight: Translated: 41862; Mature: 41862

Theoretical pI: Translated: 6.70; Mature: 6.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDFLPFSKPAIGDEEIAAVSEVLRSGWITTGPQNQALEQEFCQATGARHAIAVCSATAG
CCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCEEEEHHCCCC
MHVTLMALGIGPGDEVITPSMTWVSTLNIITLLGATPVMIDVGRDTLMVTPEAIADAITP
CEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCEEEECHHHHHHHCCC
RTKAIIPVHFAGAPVDLDPIRALAQRQGIALIEDAAHAIGTAYRGEPIGQRGTAIFSFHA
CCCEEEEEEECCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCEEEEEHH
IKNVTCAEGGMVVTDDDRLAQQVRSLKFHGLGVDAYDRQTHGRAPQAEVVTPGYKYNLAD
HCCCEECCCCEEEECCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEECCCCEEEHHH
INAAIARVQLAKLPELNARRARLAAYYLQQLAQHQLPFSPLNTPEWPHQHAWHLFIIRCD
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEEEC
EARSGVDRDRLMQALKERNIGSGLHFRAAHTQKYYRESYPQLRLAQTEWNSQRICSLPLF
CCCCCCCHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHCCCCEEEECCCCCCCCEEECCCC
PDMTEADVDRVIAALKEIIEQ
CCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKDFLPFSKPAIGDEEIAAVSEVLRSGWITTGPQNQALEQEFCQATGARHAIAVCSATAG
CCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCEEEEHHCCCC
MHVTLMALGIGPGDEVITPSMTWVSTLNIITLLGATPVMIDVGRDTLMVTPEAIADAITP
CEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCEEEECHHHHHHHCCC
RTKAIIPVHFAGAPVDLDPIRALAQRQGIALIEDAAHAIGTAYRGEPIGQRGTAIFSFHA
CCCEEEEEEECCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCEEEEEHH
IKNVTCAEGGMVVTDDDRLAQQVRSLKFHGLGVDAYDRQTHGRAPQAEVVTPGYKYNLAD
HCCCEECCCCEEEECCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEECCCCEEEHHH
INAAIARVQLAKLPELNARRARLAAYYLQQLAQHQLPFSPLNTPEWPHQHAWHLFIIRCD
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEEEC
EARSGVDRDRLMQALKERNIGSGLHFRAAHTQKYYRESYPQLRLAQTEWNSQRICSLPLF
CCCCCCCHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHCCCCEEEECCCCCCCCEEECCCC
PDMTEADVDRVIAALKEIIEQ
CCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA