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The map label for this gene is rlmL [H]

Identifier: 238919293

GI number: 238919293

Start: 1369161

End: 1371278

Strand: Direct

Name: rlmL [H]

Synonym: NT01EI_1383

Alternate gene names: 238919293

Gene position: 1369161-1371278 (Clockwise)

Preceding gene: 238919292

Following gene: 238919294

Centisome position: 35.91

GC content: 61.99

Gene sequence:

>2118_bases
ATGAAATCTCTTTTTGCCAGCACCGCGCGCGGTCTCGAAGAACTACTGAAAAATGAACTGGAAGGGCTCGGTGCGCATGA
CTGCCAGATTGTCCAGGGTGGCGTGCATTTCAGGGGAGATGGGCGGTTGCTCTATCAGAGTCTGATGTGGAGCCGCCTGG
CGTCGCGCATCATGCTGCCGCTGGGCGAGTTTAAGGTTTACAGCGATCTGGATCTGTACCTGGGCGTGCAGGCGATCGAC
TGGACGACGCTGTTTGGCATCGATAAGACCTTTGCCGTGCACTTCAGCGGCACCAATGAGGAGATCCGCAATACCCAGTA
TGGCGCCCTTAAGGTCAAGGATGCCATTGTTGACCACTTTACGCGGAGTATCGACGCGCGTCCCAGCGTGGCCAAGCAGG
ATCCCGATATCCGTATCAGCGTCTATCTGCAGCGGGAAACGGCGATCGTATCGCTGGATCTCAGCGGCGATGGGCTGCAT
CAGCGCGGCTATCGCGATGTGACCGGCCAGGCGCCGCTGAAAGAGACGCTGGCGGCCGCCGTGGTGCTGCGCTCCGGGTG
GCAGCCGGGTACGCCAATGGTCGATCCGATGTGCGGATCCGGCACGCTGCTGATCGAGGCGGCGATGATGGCATCCGATC
GGGCGCCGGGGCTGCAGCGTGAGCGCTGGGGCTTTATGGGCTGGCTCGGTCACGACGCCGATCTGTGGCAGGAGGTGCGG
GTAGAGGCGCAGCGCCGCGCCCGCTTGGGGCTGGAGCAGACGACGTCGCGCTTTTTTGGCTACGATCTCGATCGCCGCGT
GCTGGAGATGGCGCGCGCCAACGCCCGCCGCGCCGGTGTTGCCTCTCTGATCACCTTTAACGGTGGCGACGTGGCTCAGC
TGCATAACCCGCTGCCGGAAGGGCCGCTGGGGACGGTGCTGAGTAACCCACCCTATGGGGAGCGGCTGGAGAGCGAGCCG
GCGCTGATTGCGCTGCACAATACGCTGGGGCGCATCATGAAGCGGGCGTTCGGCGGCTGGCAGCTGTCGCTGTTCAGCGC
CTCGCCGGAGCTGTTGAGTTGTCTGCAGCTGCGCGCCGAGCGCCAGTTTAAGGCCAAGAATGGCCCGCTGGAGTGTGTCC
AGAAGAACTATCGCCTGAGCGCGGCCCCGCAGGCCGACGGCGGCGAGGTGGCGCAGGACTATGCCAACCGTCTGCGTAAG
AACCTGAAAAAGTTTGATAAGTGGGCGCGGCAAGAGGGGCTGACGTGCTATCGGGTTTACGATGCCGATCTGCCGGAGTA
CAACGTGGCGGTCGATCGCTACGGGGAAAAGATCGTGGTCCAGGAGTATGCGCCGCCGAAGAGCGTCGATGCGCAGAAGG
CGCGCCAGCGTCTGTTTGACGTGGTCAGCGTGACCCAGTCGGTGATGGGGGTTTCAGGCAATAATCTGATCCTGAAAACC
CGTGAGCGGCAGAAGGGTAAAAGCCAGTATGAGAAGCTGGCGCAAAAGGGCGAGTTCATGGAGGTGCAGGAGTACGGCGC
CCGTTTCTGGGTCAACCTGACCGACTATCTGGACAGCGGACTGTTTATCGACCACCGCATTGCGCGTCGGATGCTGGGCG
AGATGAGCCGCGGTAAGGATTTCCTGAACCTCTTCGCCTATACCGGCAGCGCCAGCGTGTATGCTGGGCTGGGCGGCGCG
CGTTCGACCACCACGGTCGATATGTCCCGTACCTATCTGGAGTGGGCGGAGCGCAACCTGCGCCTGAATGGGCTGACCGG
GCGTCAGCACCGCCTGGTTCAGGCGGACTGTCTGAACTATCTGCACCACTGCGAGGATAAGTTCGATCTGATCTTTATCG
ATCCGCCGACCTTTTCCAACTCGAAGCGGATGGAGGGGACCTTTGATGTGCAGCGCGATCACCTGGATCTGATGCGCGAT
CTGGCCCGGATACTGCGTCCGGGCGGTACCATCATGTTCTCCAACAACAAACGCGGTTTCAAAATGGATCTGGCCGGGCT
GGCGCAGCGGGGCCTGCAGGCGCAGGAGATCACCGATCGCACGCTCTCTGCCGACTTTGCCCGTAACCGCCAGATCCACA
ATTGCTGGCTGGTGACCCACGCCGGCGAGGAGCAGTAA

Upstream 100 bases:

>100_bases
TATTCCCCAAAACAGCGTGCTTTTGCGTCGTCCGCCAGGCGTTTCCTGCGTAGATGCGGGGAAATATCATCCATAACTGA
CTGATAGCGATAGTGAAAAC

Downstream 100 bases:

>100_bases
TTCCATGTCTTTGATTAGTCTTTCAGGCGCCTGGCTCTCTTTTAGCGATGCGCCGCTGCTGGATAATGCCGAGCTGCACA
TTGAGCCCAACGAGCGCGTC

Product: 23S rRNA m(2)G2445 methyltransferase

Products: NA

Alternate protein names: 23S rRNA m2G2445 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase rlmL [H]

Number of amino acids: Translated: 705; Mature: 705

Protein sequence:

>705_residues
MKSLFASTARGLEELLKNELEGLGAHDCQIVQGGVHFRGDGRLLYQSLMWSRLASRIMLPLGEFKVYSDLDLYLGVQAID
WTTLFGIDKTFAVHFSGTNEEIRNTQYGALKVKDAIVDHFTRSIDARPSVAKQDPDIRISVYLQRETAIVSLDLSGDGLH
QRGYRDVTGQAPLKETLAAAVVLRSGWQPGTPMVDPMCGSGTLLIEAAMMASDRAPGLQRERWGFMGWLGHDADLWQEVR
VEAQRRARLGLEQTTSRFFGYDLDRRVLEMARANARRAGVASLITFNGGDVAQLHNPLPEGPLGTVLSNPPYGERLESEP
ALIALHNTLGRIMKRAFGGWQLSLFSASPELLSCLQLRAERQFKAKNGPLECVQKNYRLSAAPQADGGEVAQDYANRLRK
NLKKFDKWARQEGLTCYRVYDADLPEYNVAVDRYGEKIVVQEYAPPKSVDAQKARQRLFDVVSVTQSVMGVSGNNLILKT
RERQKGKSQYEKLAQKGEFMEVQEYGARFWVNLTDYLDSGLFIDHRIARRMLGEMSRGKDFLNLFAYTGSASVYAGLGGA
RSTTTVDMSRTYLEWAERNLRLNGLTGRQHRLVQADCLNYLHHCEDKFDLIFIDPPTFSNSKRMEGTFDVQRDHLDLMRD
LARILRPGGTIMFSNNKRGFKMDLAGLAQRGLQAQEITDRTLSADFARNRQIHNCWLVTHAGEEQ

Sequences:

>Translated_705_residues
MKSLFASTARGLEELLKNELEGLGAHDCQIVQGGVHFRGDGRLLYQSLMWSRLASRIMLPLGEFKVYSDLDLYLGVQAID
WTTLFGIDKTFAVHFSGTNEEIRNTQYGALKVKDAIVDHFTRSIDARPSVAKQDPDIRISVYLQRETAIVSLDLSGDGLH
QRGYRDVTGQAPLKETLAAAVVLRSGWQPGTPMVDPMCGSGTLLIEAAMMASDRAPGLQRERWGFMGWLGHDADLWQEVR
VEAQRRARLGLEQTTSRFFGYDLDRRVLEMARANARRAGVASLITFNGGDVAQLHNPLPEGPLGTVLSNPPYGERLESEP
ALIALHNTLGRIMKRAFGGWQLSLFSASPELLSCLQLRAERQFKAKNGPLECVQKNYRLSAAPQADGGEVAQDYANRLRK
NLKKFDKWARQEGLTCYRVYDADLPEYNVAVDRYGEKIVVQEYAPPKSVDAQKARQRLFDVVSVTQSVMGVSGNNLILKT
RERQKGKSQYEKLAQKGEFMEVQEYGARFWVNLTDYLDSGLFIDHRIARRMLGEMSRGKDFLNLFAYTGSASVYAGLGGA
RSTTTVDMSRTYLEWAERNLRLNGLTGRQHRLVQADCLNYLHHCEDKFDLIFIDPPTFSNSKRMEGTFDVQRDHLDLMRD
LARILRPGGTIMFSNNKRGFKMDLAGLAQRGLQAQEITDRTLSADFARNRQIHNCWLVTHAGEEQ
>Mature_705_residues
MKSLFASTARGLEELLKNELEGLGAHDCQIVQGGVHFRGDGRLLYQSLMWSRLASRIMLPLGEFKVYSDLDLYLGVQAID
WTTLFGIDKTFAVHFSGTNEEIRNTQYGALKVKDAIVDHFTRSIDARPSVAKQDPDIRISVYLQRETAIVSLDLSGDGLH
QRGYRDVTGQAPLKETLAAAVVLRSGWQPGTPMVDPMCGSGTLLIEAAMMASDRAPGLQRERWGFMGWLGHDADLWQEVR
VEAQRRARLGLEQTTSRFFGYDLDRRVLEMARANARRAGVASLITFNGGDVAQLHNPLPEGPLGTVLSNPPYGERLESEP
ALIALHNTLGRIMKRAFGGWQLSLFSASPELLSCLQLRAERQFKAKNGPLECVQKNYRLSAAPQADGGEVAQDYANRLRK
NLKKFDKWARQEGLTCYRVYDADLPEYNVAVDRYGEKIVVQEYAPPKSVDAQKARQRLFDVVSVTQSVMGVSGNNLILKT
RERQKGKSQYEKLAQKGEFMEVQEYGARFWVNLTDYLDSGLFIDHRIARRMLGEMSRGKDFLNLFAYTGSASVYAGLGGA
RSTTTVDMSRTYLEWAERNLRLNGLTGRQHRLVQADCLNYLHHCEDKFDLIFIDPPTFSNSKRMEGTFDVQRDHLDLMRD
LARILRPGGTIMFSNNKRGFKMDLAGLAQRGLQAQEITDRTLSADFARNRQIHNCWLVTHAGEEQ

Specific function: Specifically methylates the guanosine in position 2445 (m2G2445) of 23S rRNA [H]

COG id: COG0116

COG function: function code L; Predicted N6-adenine-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain [H]

Homologues:

Organism=Escherichia coli, GI1787181, Length=702, Percent_Identity=74.9287749287749, Blast_Score=1097, Evalue=0.0,
Organism=Escherichia coli, GI145693111, Length=270, Percent_Identity=25.9259259259259, Blast_Score=80, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR000241
- InterPro:   IPR017244
- InterPro:   IPR019614
- InterPro:   IPR004114 [H]

Pfam domain/function: PF10672 Methyltrans_SAM; PF02926 THUMP; PF01170 UPF0020 [H]

EC number: =2.1.1.173 [H]

Molecular weight: Translated: 79361; Mature: 79361

Theoretical pI: Translated: 8.64; Mature: 8.64

Prosite motif: PS51165 THUMP ; PS00092 N6_MTASE ; PS01261 UPF0020

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSLFASTARGLEELLKNELEGLGAHDCQIVQGGVHFRGDGRLLYQSLMWSRLASRIMLP
CCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCEEEECCCHHHHHHHHHHHHHHHHHCC
LGEFKVYSDLDLYLGVQAIDWTTLFGIDKTFAVHFSGTNEEIRNTQYGALKVKDAIVDHF
CCCEEEECCCHHHCCEEEECEEEEECCCCEEEEEECCCCHHHCCCCCCEEEHHHHHHHHH
TRSIDARPSVAKQDPDIRISVYLQRETAIVSLDLSGDGLHQRGYRDVTGQAPLKETLAAA
HHHCCCCCCCCCCCCCEEEEEEEEECEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHH
VVLRSGWQPGTPMVDPMCGSGTLLIEAAMMASDRAPGLQRERWGFMGWLGHDADLWQEVR
HHHHCCCCCCCCCCCCCCCCCHHEEHHHHHHCCCCCCCCHHHCCCCEECCCCHHHHHHHH
VEAQRRARLGLEQTTSRFFGYDLDRRVLEMARANARRAGVASLITFNGGDVAQLHNPLPE
HHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHCCHHCCCEEEEEECCCCHHHHCCCCCC
GPLGTVLSNPPYGERLESEPALIALHNTLGRIMKRAFGGWQLSLFSASPELLSCLQLRAE
CCCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHH
RQFKAKNGPLECVQKNYRLSAAPQADGGEVAQDYANRLRKNLKKFDKWARQEGLTCYRVY
HHHHCCCCCHHHHHCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEE
DADLPEYNVAVDRYGEKIVVQEYAPPKSVDAQKARQRLFDVVSVTQSVMGVSGNNLILKT
CCCCCCCCCHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEC
RERQKGKSQYEKLAQKGEFMEVQEYGARFWVNLTDYLDSGLFIDHRIARRMLGEMSRGKD
CHHHHHHHHHHHHHHCCCEEEHHHCCCEEEEEEHHHHCCCCEEHHHHHHHHHHHHHCCHH
FLNLFAYTGSASVYAGLGGARSTTTVDMSRTYLEWAERNLRLNGLTGRQHRLVQADCLNY
HHHHHHCCCCCCEEECCCCCCCCEEEHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHHHH
LHHCEDKFDLIFIDPPTFSNSKRMEGTFDVQRDHLDLMRDLARILRPGGTIMFSNNKRGF
HHHCCCCCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCE
KMDLAGLAQRGLQAQEITDRTLSADFARNRQIHNCWLVTHAGEEQ
EECHHHHHHHCCCHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCC
>Mature Secondary Structure
MKSLFASTARGLEELLKNELEGLGAHDCQIVQGGVHFRGDGRLLYQSLMWSRLASRIMLP
CCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCEEEECCCHHHHHHHHHHHHHHHHHCC
LGEFKVYSDLDLYLGVQAIDWTTLFGIDKTFAVHFSGTNEEIRNTQYGALKVKDAIVDHF
CCCEEEECCCHHHCCEEEECEEEEECCCCEEEEEECCCCHHHCCCCCCEEEHHHHHHHHH
TRSIDARPSVAKQDPDIRISVYLQRETAIVSLDLSGDGLHQRGYRDVTGQAPLKETLAAA
HHHCCCCCCCCCCCCCEEEEEEEEECEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHH
VVLRSGWQPGTPMVDPMCGSGTLLIEAAMMASDRAPGLQRERWGFMGWLGHDADLWQEVR
HHHHCCCCCCCCCCCCCCCCCHHEEHHHHHHCCCCCCCCHHHCCCCEECCCCHHHHHHHH
VEAQRRARLGLEQTTSRFFGYDLDRRVLEMARANARRAGVASLITFNGGDVAQLHNPLPE
HHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHCCHHCCCEEEEEECCCCHHHHCCCCCC
GPLGTVLSNPPYGERLESEPALIALHNTLGRIMKRAFGGWQLSLFSASPELLSCLQLRAE
CCCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHH
RQFKAKNGPLECVQKNYRLSAAPQADGGEVAQDYANRLRKNLKKFDKWARQEGLTCYRVY
HHHHCCCCCHHHHHCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEE
DADLPEYNVAVDRYGEKIVVQEYAPPKSVDAQKARQRLFDVVSVTQSVMGVSGNNLILKT
CCCCCCCCCHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEC
RERQKGKSQYEKLAQKGEFMEVQEYGARFWVNLTDYLDSGLFIDHRIARRMLGEMSRGKD
CHHHHHHHHHHHHHHCCCEEEHHHCCCEEEEEEHHHHCCCCEEHHHHHHHHHHHHHCCHH
FLNLFAYTGSASVYAGLGGARSTTTVDMSRTYLEWAERNLRLNGLTGRQHRLVQADCLNY
HHHHHHCCCCCCEEECCCCCCCCEEEHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHHHH
LHHCEDKFDLIFIDPPTFSNSKRMEGTFDVQRDHLDLMRDLARILRPGGTIMFSNNKRGF
HHHCCCCCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCE
KMDLAGLAQRGLQAQEITDRTLSADFARNRQIHNCWLVTHAGEEQ
EECHHHHHHHCCCHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA