Click here to switch to the map view.

The map label for this gene is flgJ [H]

Identifier: 238919264

GI number: 238919264

Start: 1339701

End: 1340663

Strand: Direct

Name: flgJ [H]

Synonym: NT01EI_1354

Alternate gene names: 238919264

Gene position: 1339701-1340663 (Clockwise)

Preceding gene: 238919263

Following gene: 238919265

Centisome position: 35.14

GC content: 60.02

Gene sequence:

>963_bases
ATGGCTGACGATAGTCTGGCGTTTTCCGGCGCCGCGTATGATGCGCGGTCGCTGAATACGCTGAAATACAGCGCTGCCGG
AGGGTCCGATAAGGCGCTGCGGGAAACCGCACGCCAGATGGAGGGCATGTTTATCCAGATGATGCTGAAAAGCATGCGTC
AGGCGATGCCCCAGGAGGGGATCTTCGACAATGAGCAGAGCCGCCTGTATACCTCGCTCTATGATCAGCAGGTGGCTCAG
GATATGGCTGCCGGTAAAGGTATGGGGATTGCCGATATGCTGTATAAGCAGATGCGCGGTGCCCATCATGCGGCGGCGGC
GATTCCGGACAGCGTCGGGGTGACGCCGATGGCGCTGGATGGTGAAACCATTAACAGTATGCCGGTTCACGCGCTGGAGC
AGGTGATCCGTCGCGCCATGCCTCATCTCCCCCGGGCCGGTTCGGCATTACCTTTGCCCAACAGCAACGGTGAGTTTGTC
TCTCAGCTGAGCCTGCCGGCCCAGCTGGCCAGCCGCCAGAGCGGGATCCCGCATCAGCTGATTATGGCGCAGGCCGCCCT
GGAGTCGGGGTGGGGGCAACGGGAAATTCCGGGCACCAACGGCGTGCGCAGTTATAACCTGTTTGGCATCAAGGCAGGGA
AAAACTGGAATGGCCCGACCACGGAGGTGGCCACCACCGAATATGAAAATGGCGTGGCGGTGAAAACCCGGGCCCGTTTC
CGGGTATATGCGTCCTATTTTGAGGCGATCAGCGACTATGTCCGTATGCTGACCCATAATCCGCGCTACGCGGCGGTGGT
TAGCGCTGACACCCCGGAGCAGGCCGCCCATGCCCTGCAGCGGGCCGGATATGCCACCGATCCGAATTATGCCAGCAAGC
TGGTGCACCTGATAGGGCAGATAAAACAGACAGGACACCGGATGGTACAGGCCTATACCCACGATCTGGCGAATCTGTTC
TAG

Upstream 100 bases:

>100_bases
GCGCGCTGAACGCGCTGGGGGCAACGCCGAACGATCTGATGTCAATCCTGCAGGCAATGAAGAGTGCCGGTTGCCTGCGG
GCCAAGCTGGAGATTATCTG

Downstream 100 bases:

>100_bases
TCCCCGCAGCGCGGTGACGGGCGGCGAAAAAGATTTAAGTTTTGAGCCGCCCGGCCGATAAACAGAGTCACAGTGAGAGC
CCGTAGATCTCCCGGCAGCG

Product: flagellar rod assembly protein/muramidase FlgJ

Products: NA

Alternate protein names: Muramidase flgJ [H]

Number of amino acids: Translated: 320; Mature: 319

Protein sequence:

>320_residues
MADDSLAFSGAAYDARSLNTLKYSAAGGSDKALRETARQMEGMFIQMMLKSMRQAMPQEGIFDNEQSRLYTSLYDQQVAQ
DMAAGKGMGIADMLYKQMRGAHHAAAAIPDSVGVTPMALDGETINSMPVHALEQVIRRAMPHLPRAGSALPLPNSNGEFV
SQLSLPAQLASRQSGIPHQLIMAQAALESGWGQREIPGTNGVRSYNLFGIKAGKNWNGPTTEVATTEYENGVAVKTRARF
RVYASYFEAISDYVRMLTHNPRYAAVVSADTPEQAAHALQRAGYATDPNYASKLVHLIGQIKQTGHRMVQAYTHDLANLF

Sequences:

>Translated_320_residues
MADDSLAFSGAAYDARSLNTLKYSAAGGSDKALRETARQMEGMFIQMMLKSMRQAMPQEGIFDNEQSRLYTSLYDQQVAQ
DMAAGKGMGIADMLYKQMRGAHHAAAAIPDSVGVTPMALDGETINSMPVHALEQVIRRAMPHLPRAGSALPLPNSNGEFV
SQLSLPAQLASRQSGIPHQLIMAQAALESGWGQREIPGTNGVRSYNLFGIKAGKNWNGPTTEVATTEYENGVAVKTRARF
RVYASYFEAISDYVRMLTHNPRYAAVVSADTPEQAAHALQRAGYATDPNYASKLVHLIGQIKQTGHRMVQAYTHDLANLF
>Mature_319_residues
ADDSLAFSGAAYDARSLNTLKYSAAGGSDKALRETARQMEGMFIQMMLKSMRQAMPQEGIFDNEQSRLYTSLYDQQVAQD
MAAGKGMGIADMLYKQMRGAHHAAAAIPDSVGVTPMALDGETINSMPVHALEQVIRRAMPHLPRAGSALPLPNSNGEFVS
QLSLPAQLASRQSGIPHQLIMAQAALESGWGQREIPGTNGVRSYNLFGIKAGKNWNGPTTEVATTEYENGVAVKTRARFR
VYASYFEAISDYVRMLTHNPRYAAVVSADTPEQAAHALQRAGYATDPNYASKLVHLIGQIKQTGHRMVQAYTHDLANLF

Specific function: Flagellum-specific muramidase which hydrolyzes the peptidoglycan layer to assemble the rod structure in the periplasmic space [H]

COG id: COG1705

COG function: function code NU; Muramidase (flagellum-specific)

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the glycosyl hydrolase 73 family [H]

Homologues:

Organism=Escherichia coli, GI1787321, Length=323, Percent_Identity=54.1795665634675, Blast_Score=343, Evalue=1e-95,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013377
- InterPro:   IPR000423
- InterPro:   IPR019301
- InterPro:   IPR013338
- InterPro:   IPR002901 [H]

Pfam domain/function: PF01832 Glucosaminidase; PF10135 Rod-binding [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 34869; Mature: 34738

Theoretical pI: Translated: 8.65; Mature: 8.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
5.3 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADDSLAFSGAAYDARSLNTLKYSAAGGSDKALRETARQMEGMFIQMMLKSMRQAMPQEG
CCCCCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
IFDNEQSRLYTSLYDQQVAQDMAAGKGMGIADMLYKQMRGAHHAAAAIPDSVGVTPMALD
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHCCCCCCCCEEEEC
GETINSMPVHALEQVIRRAMPHLPRAGSALPLPNSNGEFVSQLSLPAQLASRQSGIPHQL
CCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHH
IMAQAALESGWGQREIPGTNGVRSYNLFGIKAGKNWNGPTTEVATTEYENGVAVKTRARF
HHHHHHHHCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCEEEEECCCCEEEEHHHHH
RVYASYFEAISDYVRMLTHNPRYAAVVSADTPEQAAHALQRAGYATDPNYASKLVHLIGQ
HHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
IKQTGHRMVQAYTHDLANLF
HHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
ADDSLAFSGAAYDARSLNTLKYSAAGGSDKALRETARQMEGMFIQMMLKSMRQAMPQEG
CCCCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
IFDNEQSRLYTSLYDQQVAQDMAAGKGMGIADMLYKQMRGAHHAAAAIPDSVGVTPMALD
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHCCCCCCCCEEEEC
GETINSMPVHALEQVIRRAMPHLPRAGSALPLPNSNGEFVSQLSLPAQLASRQSGIPHQL
CCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHH
IMAQAALESGWGQREIPGTNGVRSYNLFGIKAGKNWNGPTTEVATTEYENGVAVKTRARF
HHHHHHHHCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCEEEEECCCCEEEEHHHHH
RVYASYFEAISDYVRMLTHNPRYAAVVSADTPEQAAHALQRAGYATDPNYASKLVHLIGQ
HHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
IKQTGHRMVQAYTHDLANLF
HHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2544561; 11677609; 2193164; 10049388 [H]