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The map label for this gene is rfbB [C]

Identifier: 238919230

GI number: 238919230

Start: 1304468

End: 1305475

Strand: Direct

Name: rfbB [C]

Synonym: NT01EI_1313

Alternate gene names: 238919230

Gene position: 1304468-1305475 (Clockwise)

Preceding gene: 238919229

Following gene: 238919231

Centisome position: 34.22

GC content: 59.33

Gene sequence:

>1008_bases
ATGCGTGTTTTAGTAACTGGCTGCGCCGGCTTTATCGGTGCCAATCTCTGTGGTCGTCTGCTGGCGGCGGGGCATCAGGT
CGAGGGCATCGACAACCTGAACGACTATTACGATGTGTCGCTCAAGCAGGCGCGCCTGGCGCCGCTGCAGGCCGATGCCA
ACTTTACCTATCATCCGATCGATATCGCCGACAGCGAAGCCATGGCGGCGCTGTTTAGCGCCGCCCACTTTGACCGGGTG
GTTCACCTGGCGGCGCAGGCGGGAGTGCGCTACTCCCTGGCCAACCCGCTGAGCTATGCCCAGAGCAACCTGCTGGGACA
CGTCAACGTGCTGGAAGGCTGTCGTCACAGTAATGTGGGGCATCTAATTTACGCCTCCTCCAGCTCCGTGTATGGGTTGA
ACAACAAGGTGCCGTTCTCGACCGCCGACCGCGTCGATCACCCGATTTCCCTGTATGCCGCCACCAAGAAGTCCAACGAG
CTGATGGCGCACTCCTACTCTCATCTGTACGGGCTGCCGACCACCGGCCTGCGCTTCTTTACGGTCTATGGGCCGTGGGG
ACGCCCGGATATGGCGCTGTTCAAGTTCACCAAGAGCATGCTGGAAGGCAAGCCCATCGATATCTACAACCATGGCGATA
TGCAGCGTGACTTTACCTATATCGACGATATCGTCGAAGGCGTATTGCGTATCATGGACGTGATACCGCAGCCAAACGCC
GGGTGGCGGGTCGAGCAGGATTCACCGGCCGCCAGCTCCGCGCCATACCGTATCTATAATATCGGTCACGGCAGCCCGGT
GCGCCTGATGGACTACATCACGGCGCTGGAGGAGGCCCTGGGGATCGAGGCGCAGAAGCACTTTATGCCGATGCAGCCGG
GCGATGTCTACCAGACCTATGCCGATACCGAGGATCTGTTCGCGGTGACCGGCTACCGCCCGCAGGTGGGGGTGAAGGCG
GGGGTGCAGGCCTTTGTTGATTGGTATTCTCTGTATTATAAGATATAA

Upstream 100 bases:

>100_bases
CATAATGTCCATTGGATCATACTGACCAACACCTGCCTCCAACGCACGCCCCAGGGCGTGCGTGTCGTTTCAGCCGGGGG
AGATTTTTACAGGATAATTT

Downstream 100 bases:

>100_bases
TGTTGCGCATTGTTGTTTAAAATGTCAAAAACATATGGATCTTTATATTTGTCTTACGCCATTACAGGCTTTAATTGCTG
AAAAAATAAAGGAAAAGCAA

Product: UDP-glucuronate 5'-epimerase

Products: dTDP-4-dehydro-6-deoxy-D-glucose; H2O [C]

Alternate protein names: ORF2 [H]

Number of amino acids: Translated: 335; Mature: 335

Protein sequence:

>335_residues
MRVLVTGCAGFIGANLCGRLLAAGHQVEGIDNLNDYYDVSLKQARLAPLQADANFTYHPIDIADSEAMAALFSAAHFDRV
VHLAAQAGVRYSLANPLSYAQSNLLGHVNVLEGCRHSNVGHLIYASSSSVYGLNNKVPFSTADRVDHPISLYAATKKSNE
LMAHSYSHLYGLPTTGLRFFTVYGPWGRPDMALFKFTKSMLEGKPIDIYNHGDMQRDFTYIDDIVEGVLRIMDVIPQPNA
GWRVEQDSPAASSAPYRIYNIGHGSPVRLMDYITALEEALGIEAQKHFMPMQPGDVYQTYADTEDLFAVTGYRPQVGVKA
GVQAFVDWYSLYYKI

Sequences:

>Translated_335_residues
MRVLVTGCAGFIGANLCGRLLAAGHQVEGIDNLNDYYDVSLKQARLAPLQADANFTYHPIDIADSEAMAALFSAAHFDRV
VHLAAQAGVRYSLANPLSYAQSNLLGHVNVLEGCRHSNVGHLIYASSSSVYGLNNKVPFSTADRVDHPISLYAATKKSNE
LMAHSYSHLYGLPTTGLRFFTVYGPWGRPDMALFKFTKSMLEGKPIDIYNHGDMQRDFTYIDDIVEGVLRIMDVIPQPNA
GWRVEQDSPAASSAPYRIYNIGHGSPVRLMDYITALEEALGIEAQKHFMPMQPGDVYQTYADTEDLFAVTGYRPQVGVKA
GVQAFVDWYSLYYKI
>Mature_335_residues
MRVLVTGCAGFIGANLCGRLLAAGHQVEGIDNLNDYYDVSLKQARLAPLQADANFTYHPIDIADSEAMAALFSAAHFDRV
VHLAAQAGVRYSLANPLSYAQSNLLGHVNVLEGCRHSNVGHLIYASSSSVYGLNNKVPFSTADRVDHPISLYAATKKSNE
LMAHSYSHLYGLPTTGLRFFTVYGPWGRPDMALFKFTKSMLEGKPIDIYNHGDMQRDFTYIDDIVEGVLRIMDVIPQPNA
GWRVEQDSPAASSAPYRIYNIGHGSPVRLMDYITALEEALGIEAQKHFMPMQPGDVYQTYADTEDLFAVTGYRPQVGVKA
GVQAFVDWYSLYYKI

Specific function: DTDP-L-RHAMNOSE BIOSYNTHESIS WITHIN THE O ANTIGEN BIOSYNTHESIS PATHWAY OF LIPOPOLYSACCHARIDE BIOSYNTHESIS. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=337, Percent_Identity=26.7062314540059, Blast_Score=111, Evalue=9e-25,
Organism=Homo sapiens, GI42516563, Length=338, Percent_Identity=23.0769230769231, Blast_Score=95, Evalue=1e-19,
Organism=Homo sapiens, GI56237023, Length=349, Percent_Identity=24.0687679083095, Blast_Score=85, Evalue=8e-17,
Organism=Homo sapiens, GI56118217, Length=349, Percent_Identity=24.0687679083095, Blast_Score=85, Evalue=8e-17,
Organism=Homo sapiens, GI189083684, Length=349, Percent_Identity=24.0687679083095, Blast_Score=85, Evalue=8e-17,
Organism=Escherichia coli, GI1788353, Length=356, Percent_Identity=26.685393258427, Blast_Score=119, Evalue=4e-28,
Organism=Escherichia coli, GI48994969, Length=351, Percent_Identity=25.0712250712251, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI1786974, Length=323, Percent_Identity=26.0061919504644, Blast_Score=97, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI71982038, Length=355, Percent_Identity=25.6338028169014, Blast_Score=111, Evalue=5e-25,
Organism=Caenorhabditis elegans, GI71982035, Length=354, Percent_Identity=25.7062146892655, Blast_Score=111, Evalue=7e-25,
Organism=Caenorhabditis elegans, GI17568069, Length=238, Percent_Identity=27.7310924369748, Blast_Score=91, Evalue=9e-19,
Organism=Caenorhabditis elegans, GI17539532, Length=340, Percent_Identity=25.5882352941176, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI133901786, Length=245, Percent_Identity=26.530612244898, Blast_Score=67, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI133901788, Length=244, Percent_Identity=26.6393442622951, Blast_Score=67, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17539422, Length=245, Percent_Identity=26.530612244898, Blast_Score=67, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17539424, Length=244, Percent_Identity=26.6393442622951, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI133901790, Length=245, Percent_Identity=26.530612244898, Blast_Score=66, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6319493, Length=353, Percent_Identity=23.7960339943343, Blast_Score=77, Evalue=4e-15,
Organism=Drosophila melanogaster, GI19923002, Length=349, Percent_Identity=25.5014326647564, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI21356223, Length=256, Percent_Identity=28.515625, Blast_Score=84, Evalue=9e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040
- InterPro:   IPR008089 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 4.2.1.46 [C]

Molecular weight: Translated: 37044; Mature: 37044

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVLVTGCAGFIGANLCGRLLAAGHQVEGIDNLNDYYDVSLKQARLAPLQADANFTYHPI
CEEEEECHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCHHHHHCCCEECCCCCEEECC
DIADSEAMAALFSAAHFDRVVHLAAQAGVRYSLANPLSYAQSNLLGHVNVLEGCRHSNVG
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCHHHHHHHHHCCHHHHHHCCCCCCCC
HLIYASSSSVYGLNNKVPFSTADRVDHPISLYAATKKSNELMAHSYSHLYGLPTTGLRFF
EEEEECCCCEEECCCCCCCCCHHCCCCCEEEEEECCCCCHHHHHHHHHHCCCCCCCEEEE
TVYGPWGRPDMALFKFTKSMLEGKPIDIYNHGDMQRDFTYIDDIVEGVLRIMDVIPQPNA
EEECCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCC
GWRVEQDSPAASSAPYRIYNIGHGSPVRLMDYITALEEALGIEAQKHFMPMQPGDVYQTY
CEEECCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCHHHHH
ADTEDLFAVTGYRPQVGVKAGVQAFVDWYSLYYKI
CCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRVLVTGCAGFIGANLCGRLLAAGHQVEGIDNLNDYYDVSLKQARLAPLQADANFTYHPI
CEEEEECHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCHHHHHCCCEECCCCCEEECC
DIADSEAMAALFSAAHFDRVVHLAAQAGVRYSLANPLSYAQSNLLGHVNVLEGCRHSNVG
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCHHHHHHHHHCCHHHHHHCCCCCCCC
HLIYASSSSVYGLNNKVPFSTADRVDHPISLYAATKKSNELMAHSYSHLYGLPTTGLRFF
EEEEECCCCEEECCCCCCCCCHHCCCCCEEEEEECCCCCHHHHHHHHHHCCCCCCCEEEE
TVYGPWGRPDMALFKFTKSMLEGKPIDIYNHGDMQRDFTYIDDIVEGVLRIMDVIPQPNA
EEECCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCC
GWRVEQDSPAASSAPYRIYNIGHGSPVRLMDYITALEEALGIEAQKHFMPMQPGDVYQTY
CEEECCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCHHHHH
ADTEDLFAVTGYRPQVGVKAGVQAFVDWYSLYYKI
CCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.019 {NAD+}} 0.034 {dTDPglucose}} [C]

Substrates: dTDPglucose [C]

Specific reaction: dTDPglucose --> dTDP-4-dehydro-6-deoxy-D-glucose + H2O [C]

General reaction: Elimination (of H2O C-O bond cleavage [C]

Inhibitor: TDP; TTP [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7682279 [H]