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The map label for this gene is rfbX [H]

Identifier: 238919221

GI number: 238919221

Start: 1294276

End: 1295523

Strand: Direct

Name: rfbX [H]

Synonym: NT01EI_1304

Alternate gene names: 238919221

Gene position: 1294276-1295523 (Clockwise)

Preceding gene: 238919220

Following gene: 238919222

Centisome position: 33.95

GC content: 32.29

Gene sequence:

>1248_bases
ATGAGTTCACTTCTAAAAAATTCCATATCTTTACTTGCAATGCAAGGGATTAACTACATACTACCACTAGTAACGCTACC
TTACTTGACTAGAGTTCTGCATGTATATCCATTTGGTATATATAGTGCAACGATGAGTGTTGGCGCTTATGTAATATTGT
TCATAAATTTTGGTTTTGATCTTAGTGCGACGAGGAAAATCGCAAAAAACAAGGGTGATAATTATGCTATTAGCGCCATA
TTTTTTAATGTTATCTTGGCTAAGTTTTTGTTATTTTTTCTCTCGCTTGTTATCGTGTTTTTATTGCTTTATACGATTGA
TTCATTTTATCCTATTAGGTCTTTATTCTTTTTATTAATACCTCAGTTTATTGGCTGTGTTTTTTTTCCTGCTTGGTTAT
ATCAAGGGGTGGAGCGGTTATATCTAATATCAGCAATAACATCTGCCACAAAGCTATTAATTATTCCTCTGTTTTTTTTA
TTTGTAAGAGATGCTGGTGATATTGATAAAGCTATAATTATCTCTGGTTTCCCGCTGTTCTTTTCTGGTATCATTGGTTT
TTATCTAGCATATAAGTTAAATATCATCAACTTTTCAGATGTGAGGGTAAGGGTTAATGATGCTATTCTTTTGATAAAAG
AATCTACTTCTGTTTTTATTGGGGCTGTGGCTATAAGTATATATACGGCATGTACTCCAATTATTTTAAGCATTGTTAGT
AATTATACTGAGGTCGGCTATTTTTCAGCAGTAGATAAATTAAGAAGTGCTATTTTAGGTATGTTTATTGTGCTAGGTCA
GGTTTTTTATCCAAGAGCTACTGTTTTGTTTGAAAGTAATTTTTCAAAATATAAGCTATTTATAAGGCGAATTATTTTTT
CGCAGATTATTTTTGGCGGGGTTGGGACGATATTATTTTATTTCTTCATGCCAATCATTGCGCCATTTATTTTAGGGCGT
GGTTTTCATGGGTTGACAGAGCTCATTGAAATAATGGCCCCAATGATATTATTAATTCCTTTGTCTGTAATATTCTCTAA
TTGCATTCTACTGCCAATGGGGAAGAGTAAATTGTTTTATATTGCTCCCATAATAACAGCTATAATACATTTGCCATACT
CTATTTATTTGAGTGAAAAGTATGGCGCTTTTGGTGGCGGGGTCTCGATATTAATAACTGAGGTCCTTAGTTTAATGTTG
TTAGTGTTTTTTTCTTATAAATACGCAAATTTGAAAAGCATTTTTTGA

Upstream 100 bases:

>100_bases
TTTAATTTAATAAGGAAGTTGCTTTGTAAAATAATAAAAATATTGGTATTGAGGATAAGTTTTGATGGCTGGAAGCATTG
TTAAAATATAATGGTATATT

Downstream 100 bases:

>100_bases
AGTGAGAGGATTACATAAGGCACATGTTGTTTTATGGATTGGAGAGTCAATTAGGTGAGGGCTATATATAATTATAGGCT
TTGTTGAATCATGCTGTTTG

Product: O antigen transporter

Products: lipopolysaccharide [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 415; Mature: 414

Protein sequence:

>415_residues
MSSLLKNSISLLAMQGINYILPLVTLPYLTRVLHVYPFGIYSATMSVGAYVILFINFGFDLSATRKIAKNKGDNYAISAI
FFNVILAKFLLFFLSLVIVFLLLYTIDSFYPIRSLFFLLIPQFIGCVFFPAWLYQGVERLYLISAITSATKLLIIPLFFL
FVRDAGDIDKAIIISGFPLFFSGIIGFYLAYKLNIINFSDVRVRVNDAILLIKESTSVFIGAVAISIYTACTPIILSIVS
NYTEVGYFSAVDKLRSAILGMFIVLGQVFYPRATVLFESNFSKYKLFIRRIIFSQIIFGGVGTILFYFFMPIIAPFILGR
GFHGLTELIEIMAPMILLIPLSVIFSNCILLPMGKSKLFYIAPIITAIIHLPYSIYLSEKYGAFGGGVSILITEVLSLML
LVFFSYKYANLKSIF

Sequences:

>Translated_415_residues
MSSLLKNSISLLAMQGINYILPLVTLPYLTRVLHVYPFGIYSATMSVGAYVILFINFGFDLSATRKIAKNKGDNYAISAI
FFNVILAKFLLFFLSLVIVFLLLYTIDSFYPIRSLFFLLIPQFIGCVFFPAWLYQGVERLYLISAITSATKLLIIPLFFL
FVRDAGDIDKAIIISGFPLFFSGIIGFYLAYKLNIINFSDVRVRVNDAILLIKESTSVFIGAVAISIYTACTPIILSIVS
NYTEVGYFSAVDKLRSAILGMFIVLGQVFYPRATVLFESNFSKYKLFIRRIIFSQIIFGGVGTILFYFFMPIIAPFILGR
GFHGLTELIEIMAPMILLIPLSVIFSNCILLPMGKSKLFYIAPIITAIIHLPYSIYLSEKYGAFGGGVSILITEVLSLML
LVFFSYKYANLKSIF
>Mature_414_residues
SSLLKNSISLLAMQGINYILPLVTLPYLTRVLHVYPFGIYSATMSVGAYVILFINFGFDLSATRKIAKNKGDNYAISAIF
FNVILAKFLLFFLSLVIVFLLLYTIDSFYPIRSLFFLLIPQFIGCVFFPAWLYQGVERLYLISAITSATKLLIIPLFFLF
VRDAGDIDKAIIISGFPLFFSGIIGFYLAYKLNIINFSDVRVRVNDAILLIKESTSVFIGAVAISIYTACTPIILSIVSN
YTEVGYFSAVDKLRSAILGMFIVLGQVFYPRATVLFESNFSKYKLFIRRIIFSQIIFGGVGTILFYFFMPIIAPFILGRG
FHGLTELIEIMAPMILLIPLSVIFSNCILLPMGKSKLFYIAPIITAIIHLPYSIYLSEKYGAFGGGVSILITEVLSLMLL
VFFSYKYANLKSIF

Specific function: May be involved in the translocation process of the nascent O-polysaccharide molecules and/or its ligation to lipid A core units [H]

COG id: COG2244

COG function: function code R; Membrane protein involved in the export of O-antigen and teichoic acid

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polysaccharide synthase family [H]

Homologues:

Organism=Escherichia coli, GI1788349, Length=401, Percent_Identity=28.927680798005, Blast_Score=160, Evalue=1e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002797 [H]

Pfam domain/function: PF01943 Polysacc_synt [H]

EC number: NA

Molecular weight: Translated: 46606; Mature: 46475

Theoretical pI: Translated: 9.71; Mature: 9.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSLLKNSISLLAMQGINYILPLVTLPYLTRVLHVYPFGIYSATMSVGAYVILFINFGFD
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCC
LSATRKIAKNKGDNYAISAIFFNVILAKFLLFFLSLVIVFLLLYTIDSFYPIRSLFFLLI
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
PQFIGCVFFPAWLYQGVERLYLISAITSATKLLIIPLFFLFVRDAGDIDKAIIISGFPLF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCHHH
FSGIIGFYLAYKLNIINFSDVRVRVNDAILLIKESTSVFIGAVAISIYTACTPIILSIVS
HHHHHHHHHHHHHEEEEECCEEEEECCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHH
NYTEVGYFSAVDKLRSAILGMFIVLGQVFYPRATVLFESNFSKYKLFIRRIIFSQIIFGG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHHHHH
VGTILFYFFMPIIAPFILGRGFHGLTELIEIMAPMILLIPLSVIFSNCILLPMGKSKLFY
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCHHH
IAPIITAIIHLPYSIYLSEKYGAFGGGVSILITEVLSLMLLVFFSYKYANLKSIF
HHHHHHHHHHCCHHEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SSLLKNSISLLAMQGINYILPLVTLPYLTRVLHVYPFGIYSATMSVGAYVILFINFGFD
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCC
LSATRKIAKNKGDNYAISAIFFNVILAKFLLFFLSLVIVFLLLYTIDSFYPIRSLFFLLI
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
PQFIGCVFFPAWLYQGVERLYLISAITSATKLLIIPLFFLFVRDAGDIDKAIIISGFPLF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCHHH
FSGIIGFYLAYKLNIINFSDVRVRVNDAILLIKESTSVFIGAVAISIYTACTPIILSIVS
HHHHHHHHHHHHHEEEEECCEEEEECCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHH
NYTEVGYFSAVDKLRSAILGMFIVLGQVFYPRATVLFESNFSKYKLFIRRIIFSQIIFGG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHHHHH
VGTILFYFFMPIIAPFILGRGFHGLTELIEIMAPMILLIPLSVIFSNCILLPMGKSKLFY
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCHHH
IAPIITAIIHLPYSIYLSEKYGAFGGGVSILITEVLSLMLLVFFSYKYANLKSIF
HHHHHHHHHHCCHHEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: lipopolysaccharide [Cytoplasm] [C]

Specific reaction: lipopolysaccharide [Cytoplasm] = lipopolysaccharide [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7517390; 7517391; 9097040; 9278503 [H]