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The map label for this gene is folE

Identifier: 238919194

GI number: 238919194

Start: 1261458

End: 1262120

Strand: Direct

Name: folE

Synonym: NT01EI_1277

Alternate gene names: 238919194

Gene position: 1261458-1262120 (Clockwise)

Preceding gene: 238919190

Following gene: 238919195

Centisome position: 33.09

GC content: 57.62

Gene sequence:

>663_bases
ATGTCATCGCTAAGTAAAGAAGCTGCACTGGTGCACGAGGCGCTGCTGGCGCGCGGGTTGGAAACTCCACTGCGCGGCGA
GCCGCTGGCGCGCGATACGCGCAAGGCGCAGATTGAGGCGCACATGACCGAGGTCATGAGGCTGTTGAATCTGGATCTGT
CGGATGACAGCCTTGTAGAGACACCGTCGCGGATCGCCAAAATGTACGTGGACGAAATTTTTTCCGGCCTGGATTACGCC
AACTTTCCTAAAATCACCATTATTGAAAATAAGATGGATGTCGATGAGATGGTGACGGTGCGCGATATTACGCTGACCAG
CACCTGCGAACACCATTTCGTCACCATCGATGGCAAGGCGACGGTCGCCTACATCCCGAAAGAGAGGGTGATTGGGCTGT
CCAAGATCAACCGGATTGTCCGCTTTTTTGCCCAGCGCCCACAGGTACAGGAGCGGTTGACCCAGCAAATCCTGGTGGCG
CTGCAGACGCTGCTGGGGACCAAGAACGTCGCCGTCTCCATCGATGCGGTGCACTACTGCGTCAAAGCCCGCGGCGTACG
CGATGCGACCAGCGCGACCACCACCACCTCGCTGGGCGGGCTGTTTAAAACCAGTCAAAATACCCGCCAGGAGTTTCTGC
GCGCGGTGCGTCACCACGTTTAG

Upstream 100 bases:

>100_bases
TGTCATATCGGTGCAGAGCCAGAATGAGATCATCGGTGTGGGGAGGCTGGCGCGGGGCCGGGGTTCAGGGGCAGAGATAA
GAGGTATCGGGAGATAAGTG

Downstream 100 bases:

>100_bases
CCCGGGCTCAGAAGCATGAAGACACCGGCGCGATACCGGCGTCCTCATCTGCAAACGACGGGGAATACCACCATGGCCGG
GGTGCCTGTGGCGGCGGAGC

Product: GTP cyclohydrolase I

Products: NA

Alternate protein names: GTP cyclohydrolase I; GTP-CH-I

Number of amino acids: Translated: 220; Mature: 219

Protein sequence:

>220_residues
MSSLSKEAALVHEALLARGLETPLRGEPLARDTRKAQIEAHMTEVMRLLNLDLSDDSLVETPSRIAKMYVDEIFSGLDYA
NFPKITIIENKMDVDEMVTVRDITLTSTCEHHFVTIDGKATVAYIPKERVIGLSKINRIVRFFAQRPQVQERLTQQILVA
LQTLLGTKNVAVSIDAVHYCVKARGVRDATSATTTTSLGGLFKTSQNTRQEFLRAVRHHV

Sequences:

>Translated_220_residues
MSSLSKEAALVHEALLARGLETPLRGEPLARDTRKAQIEAHMTEVMRLLNLDLSDDSLVETPSRIAKMYVDEIFSGLDYA
NFPKITIIENKMDVDEMVTVRDITLTSTCEHHFVTIDGKATVAYIPKERVIGLSKINRIVRFFAQRPQVQERLTQQILVA
LQTLLGTKNVAVSIDAVHYCVKARGVRDATSATTTTSLGGLFKTSQNTRQEFLRAVRHHV
>Mature_219_residues
SSLSKEAALVHEALLARGLETPLRGEPLARDTRKAQIEAHMTEVMRLLNLDLSDDSLVETPSRIAKMYVDEIFSGLDYAN
FPKITIIENKMDVDEMVTVRDITLTSTCEHHFVTIDGKATVAYIPKERVIGLSKINRIVRFFAQRPQVQERLTQQILVAL
QTLLGTKNVAVSIDAVHYCVKARGVRDATSATTTTSLGGLFKTSQNTRQEFLRAVRHHV

Specific function: Tetrahydrofolate biosynthesis; first step. [C]

COG id: COG0302

COG function: function code H; GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family

Homologues:

Organism=Homo sapiens, GI66932968, Length=187, Percent_Identity=37.9679144385027, Blast_Score=123, Evalue=1e-28,
Organism=Homo sapiens, GI4503949, Length=187, Percent_Identity=37.9679144385027, Blast_Score=123, Evalue=1e-28,
Organism=Homo sapiens, GI66932970, Length=146, Percent_Identity=37.6712328767123, Blast_Score=90, Evalue=1e-18,
Organism=Homo sapiens, GI66932972, Length=146, Percent_Identity=37.6712328767123, Blast_Score=90, Evalue=2e-18,
Organism=Escherichia coli, GI1788476, Length=221, Percent_Identity=86.4253393665158, Blast_Score=376, Evalue=1e-106,
Organism=Caenorhabditis elegans, GI17560486, Length=172, Percent_Identity=41.2790697674419, Blast_Score=129, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6321706, Length=182, Percent_Identity=39.5604395604396, Blast_Score=127, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24656796, Length=188, Percent_Identity=39.3617021276596, Blast_Score=114, Evalue=7e-26,
Organism=Drosophila melanogaster, GI24656782, Length=188, Percent_Identity=39.3617021276596, Blast_Score=113, Evalue=7e-26,
Organism=Drosophila melanogaster, GI24656791, Length=188, Percent_Identity=39.3617021276596, Blast_Score=113, Evalue=1e-25,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): GCH1_EDWI9 (C5BCL9)

Other databases:

- EMBL:   CP001600
- RefSeq:   YP_002932709.1
- GeneID:   7960607
- GenomeReviews:   CP001600_GR
- KEGG:   eic:NT01EI_1277
- OMA:   ARFMSMI
- ProtClustDB:   PRK09347
- GO:   GO:0005737
- HAMAP:   MF_00223
- InterPro:   IPR001474
- InterPro:   IPR020602
- InterPro:   IPR018234
- TIGRFAMs:   TIGR00063

Pfam domain/function: PF01227 GTP_cyclohydroI

EC number: =3.5.4.16

Molecular weight: Translated: 24618; Mature: 24487

Theoretical pI: Translated: 8.71; Mature: 8.71

Prosite motif: PS00859 GTP_CYCLOHYDROL_1_1; PS00860 GTP_CYCLOHYDROL_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSLSKEAALVHEALLARGLETPLRGEPLARDTRKAQIEAHMTEVMRLLNLDLSDDSLVE
CCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
TPSRIAKMYVDEIFSGLDYANFPKITIIENKMDVDEMVTVRDITLTSTCEHHFVTIDGKA
HHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHEEEECCCCCEEEEECCCE
TVAYIPKERVIGLSKINRIVRFFAQRPQVQERLTQQILVALQTLLGTKNVAVSIDAVHYC
EEEECCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHH
VKARGVRDATSATTTTSLGGLFKTSQNTRQEFLRAVRHHV
HHHCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SSLSKEAALVHEALLARGLETPLRGEPLARDTRKAQIEAHMTEVMRLLNLDLSDDSLVE
CCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
TPSRIAKMYVDEIFSGLDYANFPKITIIENKMDVDEMVTVRDITLTSTCEHHFVTIDGKA
HHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHEEEECCCCCEEEEECCCE
TVAYIPKERVIGLSKINRIVRFFAQRPQVQERLTQQILVALQTLLGTKNVAVSIDAVHYC
EEEECCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHH
VKARGVRDATSATTTTSLGGLFKTSQNTRQEFLRAVRHHV
HHHCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA