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The map label for this gene is dhaK [H]

Identifier: 238919155

GI number: 238919155

Start: 1222180

End: 1222482

Strand: Direct

Name: dhaK [H]

Synonym: NT01EI_1226

Alternate gene names: 238919155

Gene position: 1222180-1222482 (Clockwise)

Preceding gene: 238919154

Following gene: 238919156

Centisome position: 32.06

GC content: 57.1

Gene sequence:

>303_bases
ATGATTAACGATCCGAACCTCGTGGTTGAAGAGGCGATTCAAGGGTACCTGCGGGCCTATCCTGAGTATTACCGTCGGAC
GGAACATCCCCGGGTGCTGGCGCATCCGTCGGTGCCGATAGCGGGAAAAGTGGGGGTGGTCTCCGGCGGTGGATCTGGTC
ATGAGCCTGCATTTTTGGGCTACGTGGGTAAAAATTTACTGGATGCCGTCGCCATCGGTGAGATTTTTTCATCGCCGACC
TCTGGCGCGTTTCTGGATGCCTTCCGAGCAGCGCACGGCGGTGAAGGGGTTGTCTGCCTATAA

Upstream 100 bases:

>100_bases
AGCATGGTTACCGGGGCTAATTTACGGGTTGACGGCGGCTACACCATCCAATAACGGGGATACCCGTTGCGAAATGACAG
TGAGGTAGGTGATGAACAGA

Downstream 100 bases:

>100_bases
TGGTAATTATGCCGGTGACAATATGAATGTTGCGTTGGCGATCAAAAAAGCGGCGGTCGAGGGGATCGTGGTTAAAACGG
TGGGTAGCCAATGACGATGT

Product: Dak kinase

Products: ADP; glycerone phosphate

Alternate protein names: NA

Number of amino acids: Translated: 100; Mature: 100

Protein sequence:

>100_residues
MINDPNLVVEEAIQGYLRAYPEYYRRTEHPRVLAHPSVPIAGKVGVVSGGGSGHEPAFLGYVGKNLLDAVAIGEIFSSPT
SGAFLDAFRAAHGGEGVVCL

Sequences:

>Translated_100_residues
MINDPNLVVEEAIQGYLRAYPEYYRRTEHPRVLAHPSVPIAGKVGVVSGGGSGHEPAFLGYVGKNLLDAVAIGEIFSSPT
SGAFLDAFRAAHGGEGVVCL
>Mature_100_residues
MINDPNLVVEEAIQGYLRAYPEYYRRTEHPRVLAHPSVPIAGKVGVVSGGGSGHEPAFLGYVGKNLLDAVAIGEIFSSPT
SGAFLDAFRAAHGGEGVVCL

Specific function: Dihydroxyacetone binding subunit of the dihydroxyacetone kinase, which is responsible for phosphorylating dihydroxyacetone. Binds covalently dihydroxyacetone in hemiaminal linkage. Acts also as a corepressor of dhaR by binding to its sensor domain, in the

COG id: COG2376

COG function: function code G; Dihydroxyacetone kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DhaK domain [H]

Homologues:

Organism=Escherichia coli, GI87081857, Length=100, Percent_Identity=39, Blast_Score=79, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004006
- InterPro:   IPR012736 [H]

Pfam domain/function: PF02733 Dak1 [H]

EC number: 2.7.1.29

Molecular weight: Translated: 10509; Mature: 10509

Theoretical pI: Translated: 6.07; Mature: 6.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINDPNLVVEEAIQGYLRAYPEYYRRTEHPRVLAHPSVPIAGKVGVVSGGGSGHEPAFLG
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCEEEEECCCCCCCCHHHH
YVGKNLLDAVAIGEIFSSPTSGAFLDAFRAAHGGEGVVCL
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCEEEC
>Mature Secondary Structure
MINDPNLVVEEAIQGYLRAYPEYYRRTEHPRVLAHPSVPIAGKVGVVSGGGSGHEPAFLG
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCEEEEECCCCCCCCHHHH
YVGKNLLDAVAIGEIFSSPTSGAFLDAFRAAHGGEGVVCL
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; glycerone

Specific reaction: ATP + glycerone = ADP + glycerone phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]