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The map label for this gene is ypfH [H]

Identifier: 238919133

GI number: 238919133

Start: 1198786

End: 1199472

Strand: Direct

Name: ypfH [H]

Synonym: NT01EI_1204

Alternate gene names: 238919133

Gene position: 1198786-1199472 (Clockwise)

Preceding gene: 238919132

Following gene: 238919138

Centisome position: 31.45

GC content: 66.38

Gene sequence:

>687_bases
ATGGAACATCATCATCACGTTGTGCAACAGCCGACAGGGACGGCGCAGCAGCTGATTCTGCTGTTTCACGCCGTAGGCGA
TAACCCGGTCGCCATGGGGCAGATTGGGGAATACTTCGCCCGGGCATTCCCACTGGCGCTGGTGGTCAGCGTCGGGTCGC
CTGAGCCGTCGCCGGATGGCGCAGGGCTGCAGTGGTATGCGCAAGAGGGGGAGGATGACGAGAGCCGTCCCGGGCGTATT
GCCGCCGCGTTGCCGCGGCTGATCGCGCTGGTGCGTCACTGGCAGCGGGTCAGTGGCGTCGGCTATGCCGCGACGGCGCT
GGTGGGTTTCTCTCAGGGGGCGACGATGGCGCTGGAGGCGGTGCGCAGCGAGCCGGATCTGGCTGGCCGGGTGATCGCGT
TCAGCGGGCGCTTTGCCACCCTGCCGCAGCATAAGCTGGGTAATACAGTGGTGCATTTGCTGCATGGCAGCGAGGATCCC
CGGGTCAGTCTGGCGCACGCGCAGGAGGCCGAGCTCCGGCTGCGCAGCACCGGCACCGATGTCACGCTGGATATTGAGCC
GGTGGGACATGCGATCAACGCGGCCCTGATGGAGGCGGCGCTGGAGCGGATGCGCACCTATATTCCGCAGCGTTACTGGG
ATGAGGCGCTCAGCGGCAAACGCGGCGAGATCGTCGCCTACCGCTGA

Upstream 100 bases:

>100_bases
CACCCGGCCGGGAGGCGCGCGGACAGCGTGGCTTTTCTCCTGGCGCAGCAGGCGTATAGTAGGAAACAAGAGCATCCGCA
TGAGTGATTAGGAGCTGCGT

Downstream 100 bases:

>100_bases
GGGGGAAGGGGCGCTATTTGCGCGGCGCCTTGTCCTGTCGGGGCCAGTCATCGTCGTCCCACTGCGCATTGTTGTCGCGG
TGCGGCGGCAGCGGCGGGCG

Product: esterase YpfH

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 228; Mature: 228

Protein sequence:

>228_residues
MEHHHHVVQQPTGTAQQLILLFHAVGDNPVAMGQIGEYFARAFPLALVVSVGSPEPSPDGAGLQWYAQEGEDDESRPGRI
AAALPRLIALVRHWQRVSGVGYAATALVGFSQGATMALEAVRSEPDLAGRVIAFSGRFATLPQHKLGNTVVHLLHGSEDP
RVSLAHAQEAELRLRSTGTDVTLDIEPVGHAINAALMEAALERMRTYIPQRYWDEALSGKRGEIVAYR

Sequences:

>Translated_228_residues
MEHHHHVVQQPTGTAQQLILLFHAVGDNPVAMGQIGEYFARAFPLALVVSVGSPEPSPDGAGLQWYAQEGEDDESRPGRI
AAALPRLIALVRHWQRVSGVGYAATALVGFSQGATMALEAVRSEPDLAGRVIAFSGRFATLPQHKLGNTVVHLLHGSEDP
RVSLAHAQEAELRLRSTGTDVTLDIEPVGHAINAALMEAALERMRTYIPQRYWDEALSGKRGEIVAYR
>Mature_228_residues
MEHHHHVVQQPTGTAQQLILLFHAVGDNPVAMGQIGEYFARAFPLALVVSVGSPEPSPDGAGLQWYAQEGEDDESRPGRI
AAALPRLIALVRHWQRVSGVGYAATALVGFSQGATMALEAVRSEPDLAGRVIAFSGRFATLPQHKLGNTVVHLLHGSEDP
RVSLAHAQEAELRLRSTGTDVTLDIEPVGHAINAALMEAALERMRTYIPQRYWDEALSGKRGEIVAYR

Specific function: Displays esterase activity toward palmitoyl-CoA and pNP- butyrate [H]

COG id: COG0400

COG function: function code R; Predicted esterase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase 2 family [H]

Homologues:

Organism=Escherichia coli, GI87082111, Length=222, Percent_Identity=56.3063063063063, Blast_Score=248, Evalue=2e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003140 [H]

Pfam domain/function: PF02230 Abhydrolase_2 [H]

EC number: 3.1.-.- [C]

Molecular weight: Translated: 24721; Mature: 24721

Theoretical pI: Translated: 6.44; Mature: 6.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEHHHHVVQQPTGTAQQLILLFHAVGDNPVAMGQIGEYFARAFPLALVVSVGSPEPSPDG
CCCCCCHHCCCCCHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHEEEECCCCCCCCCC
AGLQWYAQEGEDDESRPGRIAAALPRLIALVRHWQRVSGVGYAATALVGFSQGATMALEA
CCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHH
VRSEPDLAGRVIAFSGRFATLPQHKLGNTVVHLLHGSEDPRVSLAHAQEAELRLRSTGTD
HHCCCCCCCEEEEECCCEECCCCHHHCHHHEEEEECCCCCCEEECCCHHHHEEEECCCCE
VTLDIEPVGHAINAALMEAALERMRTYIPQRYWDEALSGKRGEIVAYR
EEEEECCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCEEEEC
>Mature Secondary Structure
MEHHHHVVQQPTGTAQQLILLFHAVGDNPVAMGQIGEYFARAFPLALVVSVGSPEPSPDG
CCCCCCHHCCCCCHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHEEEECCCCCCCCCC
AGLQWYAQEGEDDESRPGRIAAALPRLIALVRHWQRVSGVGYAATALVGFSQGATMALEA
CCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHH
VRSEPDLAGRVIAFSGRFATLPQHKLGNTVVHLLHGSEDPRVSLAHAQEAELRLRSTGTD
HHCCCCCCCEEEEECCCEECCCCHHHCHHHEEEEECCCCCCEEECCCHHHHEEEECCCCE
VTLDIEPVGHAINAALMEAALERMRTYIPQRYWDEALSGKRGEIVAYR
EEEEECCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]