Click here to switch to the map view.

The map label for this gene is dapA

Identifier: 238919129

GI number: 238919129

Start: 1193328

End: 1194209

Strand: Direct

Name: dapA

Synonym: NT01EI_1200

Alternate gene names: 238919129

Gene position: 1193328-1194209 (Clockwise)

Preceding gene: 238919126

Following gene: 238919130

Centisome position: 31.3

GC content: 57.82

Gene sequence:

>882_bases
ATGTTCACGGGAAGTATTGTAGCGCTGGTAACGCCGATGGATGACAAGGGGAATGTCGATCGCGCGAGCCTGAGAAAGTT
GGTTGATTACCATGTCGCCAGCGGGACGTCCGCCATTGTTGCCGTGGGCACCACCGGTGAGTCCGCCACGCTGAGCCATG
ACGAGCATGTCAACGTGGTTTTACAGACACTGGAGCTGGCAGATGGCCGGATTCCGGTGATCGCAGGGGCTGGCGCTAAC
GCCACCGCCGAGGCCATTGCGTTGACCGAGTGTTTTAATCACTGCGGAGTGGTGGGATGTCTGTCGGTCACGCCGTATTA
TAATAAGCCGACGCAGGAAGGGTTGTATCAGCACTTCCGTGCCATCGCCTCTTGTACCGATCTGCCGCAGATTCTGTATA
ACGTGCCGTCGCGCACCGGTTGCGATATGCTGCCAGAGACCGTGGCACGTTTGGCGCAGATTAACAATATTATCGCGATC
AAAGAAGCGACCGGGAACTTAAGCCGCGTTGGGCAGATCCAAGATCTGGTCAACGACGATCGCTTTATCCTGCTCAGTGG
TGATGATGCGTCGGCGCTGGACTTTATGTGTCTGGGCGGAAAAGGGGTTATCTCGGTAACTGCGAACGTGGCCGCGCGCC
AGATGGCGGAAATGTGCCGCCTGACGCTGGCCGGGGATGTCCATGCCGCCCGCAGGTTAAACCAGAGCCTGATGCCGCTA
CATCGCGATCTGTTTATAGAAGCAAACCCGATACCGGTAAAATGGGCCACCAAGGTATTGGGACTGATAGCGACCGACAC
CCTGCGTCTGCCGCTGACACCACTGAGCGCCAGTGCGCGCCCGGCGGTTGAACGCGCACTGCAACATGCCGGTGTGCTGT
AA

Upstream 100 bases:

>100_bases
AAAAAAGTATGCTACAAGCAATTAGCATTCAGATGTCTACTTGCGTTGACAATGCTTTAAAACGTACCATGAGACGCCTG
TTTTTAGAGGGGAATGGACT

Downstream 100 bases:

>100_bases
CTCTTAGGGAGATTGAATGGCTTACTCAGTACAAAAGTCGACGATTGCGACCCTGGTTGGCGTGTCGCTGGCGATGATGC
TCTCTGCCTGCTCCAGTGAT

Product: dihydrodipicolinate synthase

Products: NA

Alternate protein names: DHDPS

Number of amino acids: Translated: 293; Mature: 293

Protein sequence:

>293_residues
MFTGSIVALVTPMDDKGNVDRASLRKLVDYHVASGTSAIVAVGTTGESATLSHDEHVNVVLQTLELADGRIPVIAGAGAN
ATAEAIALTECFNHCGVVGCLSVTPYYNKPTQEGLYQHFRAIASCTDLPQILYNVPSRTGCDMLPETVARLAQINNIIAI
KEATGNLSRVGQIQDLVNDDRFILLSGDDASALDFMCLGGKGVISVTANVAARQMAEMCRLTLAGDVHAARRLNQSLMPL
HRDLFIEANPIPVKWATKVLGLIATDTLRLPLTPLSASARPAVERALQHAGVL

Sequences:

>Translated_293_residues
MFTGSIVALVTPMDDKGNVDRASLRKLVDYHVASGTSAIVAVGTTGESATLSHDEHVNVVLQTLELADGRIPVIAGAGAN
ATAEAIALTECFNHCGVVGCLSVTPYYNKPTQEGLYQHFRAIASCTDLPQILYNVPSRTGCDMLPETVARLAQINNIIAI
KEATGNLSRVGQIQDLVNDDRFILLSGDDASALDFMCLGGKGVISVTANVAARQMAEMCRLTLAGDVHAARRLNQSLMPL
HRDLFIEANPIPVKWATKVLGLIATDTLRLPLTPLSASARPAVERALQHAGVL
>Mature_293_residues
MFTGSIVALVTPMDDKGNVDRASLRKLVDYHVASGTSAIVAVGTTGESATLSHDEHVNVVLQTLELADGRIPVIAGAGAN
ATAEAIALTECFNHCGVVGCLSVTPYYNKPTQEGLYQHFRAIASCTDLPQILYNVPSRTGCDMLPETVARLAQINNIIAI
KEATGNLSRVGQIQDLVNDDRFILLSGDDASALDFMCLGGKGVISVTANVAARQMAEMCRLTLAGDVHAARRLNQSLMPL
HRDLFIEANPIPVKWATKVLGLIATDTLRLPLTPLSASARPAVERALQHAGVL

Specific function: Biosynthesis of diaminopimelate and lysine from aspartate semialdehyde; first step. [C]

COG id: COG0329

COG function: function code EM; Dihydrodipicolinate synthase/N-acetylneuraminate lyase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DHDPS family

Homologues:

Organism=Homo sapiens, GI31543060, Length=232, Percent_Identity=25.8620689655172, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI13540533, Length=289, Percent_Identity=24.5674740484429, Blast_Score=74, Evalue=1e-13,
Organism=Escherichia coli, GI1788823, Length=293, Percent_Identity=75.7679180887372, Blast_Score=461, Evalue=1e-131,
Organism=Escherichia coli, GI87082415, Length=299, Percent_Identity=29.7658862876254, Blast_Score=139, Evalue=2e-34,
Organism=Escherichia coli, GI1786463, Length=241, Percent_Identity=29.045643153527, Blast_Score=112, Evalue=2e-26,
Organism=Escherichia coli, GI1789620, Length=288, Percent_Identity=27.0833333333333, Blast_Score=111, Evalue=7e-26,

Paralogues:

None

Copy number: 840 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): DAPA_EDWI9 (C5B7A2)

Other databases:

- EMBL:   CP001600
- RefSeq:   YP_002932643.1
- ProteinModelPortal:   C5B7A2
- GeneID:   7960946
- GenomeReviews:   CP001600_GR
- KEGG:   eic:NT01EI_1200
- OMA:   GMYRHFK
- ProtClustDB:   CLSK2812993
- GO:   GO:0005737
- HAMAP:   MF_00418
- InterPro:   IPR013785
- InterPro:   IPR005263
- InterPro:   IPR002220
- InterPro:   IPR020625
- InterPro:   IPR020624
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR12128
- PRINTS:   PR00146
- TIGRFAMs:   TIGR00674

Pfam domain/function: PF00701 DHDPS

EC number: =4.2.1.52

Molecular weight: Translated: 31134; Mature: 31134

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: PS00665 DHDPS_1; PS00666 DHDPS_2

Important sites: ACT_SITE 161-161 BINDING 106-106

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFTGSIVALVTPMDDKGNVDRASLRKLVDYHVASGTSAIVAVGTTGESATLSHDEHVNVV
CCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCHHHHHH
LQTLELADGRIPVIAGAGANATAEAIALTECFNHCGVVGCLSVTPYYNKPTQEGLYQHFR
HHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHCCEEEEEEECCCCCCCHHHHHHHHHH
AIASCTDLPQILYNVPSRTGCDMLPETVARLAQINNIIAIKEATGNLSRVGQIQDLVNDD
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHHHCCC
RFILLSGDDASALDFMCLGGKGVISVTANVAARQMAEMCRLTLAGDVHAARRLNQSLMPL
CEEEEECCCCCCEEEEEECCCCEEEEHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCH
HRDLFIEANPIPVKWATKVLGLIATDTLRLPLTPLSASARPAVERALQHAGVL
HHHEEEECCCCCHHHHHHHHHHHHHCCEEECCCCCCCCCCHHHHHHHHHCCCC
>Mature Secondary Structure
MFTGSIVALVTPMDDKGNVDRASLRKLVDYHVASGTSAIVAVGTTGESATLSHDEHVNVV
CCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCHHHHHH
LQTLELADGRIPVIAGAGANATAEAIALTECFNHCGVVGCLSVTPYYNKPTQEGLYQHFR
HHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHCCEEEEEEECCCCCCCHHHHHHHHHH
AIASCTDLPQILYNVPSRTGCDMLPETVARLAQINNIIAIKEATGNLSRVGQIQDLVNDD
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHHHCCC
RFILLSGDDASALDFMCLGGKGVISVTANVAARQMAEMCRLTLAGDVHAARRLNQSLMPL
CEEEEECCCCCCEEEEEECCCCEEEEHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCH
HRDLFIEANPIPVKWATKVLGLIATDTLRLPLTPLSASARPAVERALQHAGVL
HHHEEEECCCCCHHHHHHHHHHHHHCCEEECCCCCCCCCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA