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The map label for this gene is dsbA [H]

Identifier: 238919087

GI number: 238919087

Start: 1150445

End: 1151083

Strand: Direct

Name: dsbA [H]

Synonym: NT01EI_1157

Alternate gene names: 238919087

Gene position: 1150445-1151083 (Clockwise)

Preceding gene: 238919086

Following gene: 238919089

Centisome position: 30.18

GC content: 55.87

Gene sequence:

>639_bases
ATGATGAAAAAGGGACTCTTCCAGGCCGCCGTCGGCCTGCTGGTACTGTGCTCTGTTGCCGCTCAGGCCGCTGGGTATCA
GGAAGGAAAACAGTATACCAACATGCCTAAAGCCGTGCCTGGAGCACCTGCGGTCGTCGAATTTTTCTCTTTCTATTGCC
CGCCCTGTAACCAGTTTGCCAACGTCTACCGCATCGGTGAGGCGGTAGACGGGATCCTGTCGCAAGGGGAGAAGGTGGTG
AAATATCACGTGAGCTTCCTTGGGCCACAAGGGGTGGCGCTGACGGAGGCGTGGTCTGTGGCGCAGGCCCTTGGCGTGTC
GGATAAGGTGGAAAAGCCGCTGTTTGATGCGGTGCAGGTGAAGCGTAGTATTAACTCTCCGGCGGATATCCGTCAGGTAT
TTATCGACTCCGGCGTTGCGGCGGCAGAGTATGATGCGGCTCTGAACAGTTTTGTCGTAAAATCGCTGACGGCCCGACAG
GAGAATGCGGTGCAGGCTTTTGGCGTGCGTGGCACCCCGTCGTTTTATGTTGCAGGCAAGTACCAGGTTAAAAATGATGG
AATGCAGGCGACCAGTATTGACGGCTATCGCAGTGAGTATGCGGACGTGGTGAAGTTCCTGCTGGCTCAGGGTAAATAG

Upstream 100 bases:

>100_bases
TGTATCAGTGAGTAATTAGGTGTAATTAATTGTAAATCAGTACAGCGTTCGCCGTGCGGAATGTTACTCTGCGCATAATT
TAACTTGATACGGTAAAGTG

Downstream 100 bases:

>100_bases
TTATCTCCTCTGCTGATAAATGCCGACCCTGTACACGATTCTGTGTAAATGCCTTTTCTCAGAAGTGACCGTCCAGACGA
TCACCGAACTCGATAATAAA

Product: thiol:disulfide interchange protein DsbA

Products: 3-oxo-D 4-steroid; protein with oxidized disulfide bonds [C]

Alternate protein names: NA

Number of amino acids: Translated: 212; Mature: 212

Protein sequence:

>212_residues
MMKKGLFQAAVGLLVLCSVAAQAAGYQEGKQYTNMPKAVPGAPAVVEFFSFYCPPCNQFANVYRIGEAVDGILSQGEKVV
KYHVSFLGPQGVALTEAWSVAQALGVSDKVEKPLFDAVQVKRSINSPADIRQVFIDSGVAAAEYDAALNSFVVKSLTARQ
ENAVQAFGVRGTPSFYVAGKYQVKNDGMQATSIDGYRSEYADVVKFLLAQGK

Sequences:

>Translated_212_residues
MMKKGLFQAAVGLLVLCSVAAQAAGYQEGKQYTNMPKAVPGAPAVVEFFSFYCPPCNQFANVYRIGEAVDGILSQGEKVV
KYHVSFLGPQGVALTEAWSVAQALGVSDKVEKPLFDAVQVKRSINSPADIRQVFIDSGVAAAEYDAALNSFVVKSLTARQ
ENAVQAFGVRGTPSFYVAGKYQVKNDGMQATSIDGYRSEYADVVKFLLAQGK
>Mature_212_residues
MMKKGLFQAAVGLLVLCSVAAQAAGYQEGKQYTNMPKAVPGAPAVVEFFSFYCPPCNQFANVYRIGEAVDGILSQGEKVV
KYHVSFLGPQGVALTEAWSVAQALGVSDKVEKPLFDAVQVKRSINSPADIRQVFIDSGVAAAEYDAALNSFVVKSLTARQ
ENAVQAFGVRGTPSFYVAGKYQVKNDGMQATSIDGYRSEYADVVKFLLAQGK

Specific function: Required for disulfide bond formation in some periplasmic proteins such as PhoA or OmpA. Acts by transferring its disulfide bond to other proteins and is reduced in the process. DsbA is reoxidized by DsbB. It is required for pilus biogenesis [H]

COG id: COG0526

COG function: function code OC; Thiol-disulfide isomerase and thioredoxins

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 thioredoxin domain [H]

Homologues:

Organism=Escherichia coli, GI1790291, Length=205, Percent_Identity=50.2439024390244, Blast_Score=203, Evalue=9e-54,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 1348 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001853
- InterPro:   IPR023205
- InterPro:   IPR017936
- InterPro:   IPR012336
- InterPro:   IPR017937
- InterPro:   IPR012335 [H]

Pfam domain/function: PF01323 DSBA [H]

EC number: NA

Molecular weight: Translated: 22737; Mature: 22737

Theoretical pI: Translated: 8.42; Mature: 8.42

Prosite motif: PS00194 THIOREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMKKGLFQAAVGLLVLCSVAAQAAGYQEGKQYTNMPKAVPGAPAVVEFFSFYCPPCNQFA
CCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCHHHHH
NVYRIGEAVDGILSQGEKVVKYHVSFLGPQGVALTEAWSVAQALGVSDKVEKPLFDAVQV
HHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHH
KRSINSPADIRQVFIDSGVAAAEYDAALNSFVVKSLTARQENAVQAFGVRGTPSFYVAGK
HHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEE
YQVKNDGMQATSIDGYRSEYADVVKFLLAQGK
EEECCCCCCCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MMKKGLFQAAVGLLVLCSVAAQAAGYQEGKQYTNMPKAVPGAPAVVEFFSFYCPPCNQFA
CCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCHHHHH
NVYRIGEAVDGILSQGEKVVKYHVSFLGPQGVALTEAWSVAQALGVSDKVEKPLFDAVQV
HHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHH
KRSINSPADIRQVFIDSGVAAAEYDAALNSFVVKSLTARQENAVQAFGVRGTPSFYVAGK
HHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEE
YQVKNDGMQATSIDGYRSEYADVVKFLLAQGK
EEECCCCCCCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-oxo-D 5-steroid; protein with reduced SH groups [C]

Specific reaction: 3-oxo-D 5-steroid = 3-oxo-D 4-steroid protein with reduced SH groups = protein with oxidized disulfide bonds [C]

General reaction: Isomerases; Intramolecular Oxidoreductases; Transposing S-S Bonds [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10463161 [H]