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The map label for this gene is tig

Identifier: 238919010

GI number: 238919010

Start: 1072792

End: 1074093

Strand: Direct

Name: tig

Synonym: NT01EI_1077

Alternate gene names: 238919010

Gene position: 1072792-1074093 (Clockwise)

Preceding gene: 238919009

Following gene: 238919011

Centisome position: 28.14

GC content: 54.99

Gene sequence:

>1302_bases
ATGCAAGTTTCTGTTGAAACCACCGAAGGCCTGGGTCGTCGCGTAAATATTACTGTTACTGCTGACAGCATCGAAAAAGC
AGTAGAGAGCGAACTGCGTAATGTTGCCAAGAAGGCACGCATTGACGGTTTCCGTAAGGGCAAAGTGCCGATGAACATCG
TCGCTCAGCGTTATGGCGCATCTGTACGTCAGGACGTGCTGGGCGATCTGATGCAGCGTAATTTCGTCGATGCGATCATC
AAAGAAAAACTGAACCCGGTCGGTGCTCCGAAGTATGTCCCGGGCGAATACAAGTTGGGTGAAGACTTTGCCTATGCGGT
AGAGTTTGAAGTTTACCCGGAGATCGAGCTGAAGGGTCTGGACACCATCGTGGTTGAGAAGCCGCAGGTTGATGTTAACG
ACGCCGACGTAGATGCGATGATCGAAACCCTGCGTAAGCAGCAGGCTTCCTGGAGTGTTAAAGAGGGCGAAGTGGGCGCA
GAGGATCGTGCGACCATCGATTTCACCGGTTCTATCGACGGCGAAGCCTTCGAAGGCGGCAAGGCGACTGACTTTGTCCT
GGCCATGGGTCAGGGCCGTATGATCCCGGGCTTCGAAGACGGTATTCTGGGTCACAAAGCCGGTGAAGAGTTCGTTATCG
ATGTCACCTTCCCGGAAGACTATCACGCTGAAAACCTGAAGGGTAAAGCGGCCAAGTTCGACATCGTACTGAAGAAAGTC
GAAGAGCGCGAACTGCCGGAGATGACCGCTGAGTTCATCAAACGCTTTGGCGTTGCCGATGGTTCTCTGGATGGCCTGAA
GGCCGAAGTGCGTAAGAACATGACCCGCGAGCTGAAAAACGCCGTGCGTAACCGCATCAAGGCCCAGGTTCTGGACGGCC
TGGTTCTGGCTAACGAAATCGACGTTCCGGCTGCGCTGGTTGACGGTGAAATCGACGTGCTGCGCCGTCAGGCCGCACAG
CGCTTCGGTGGTAACGAGCAGCAGGCGATGGAGCTGCCGCGCGAGCTGTTCGAAGAGCAGGCGAAGCGTCGCGTTATCGT
TGGTCTGCTGTTGGGCGAAGTGATCAGCAAGAGCGAGCTGAAAGCCGATGACGCCCGCGTAAATGCACTGATCGAAGAGA
TGGCTTCTGCCTACGAAGATCCGCAGGAAGTGATCGAGTTCTACAGCAAGAACAAAGAGCTGATGAACAACATGCGCAAC
ATGGCGCTGGAAGAGCAGGCGGTTGAAGCTCTGCTGTCCCAGGCTCAAGTCAGCGAAAAAGCGACCACCTTCAACGAACT
GATGAACCAGCCGCAGGCGTAA

Upstream 100 bases:

>100_bases
TTTTGCGCTATCCCACTTCAGAGTAAGTTGCGGCTCTCAGGCATGATGCAGAGAACGGCTTCTGGCGTTGACCGAGCACT
GTGATTTTTTGAGGTATAAG

Downstream 100 bases:

>100_bases
GCCCCAGGCCATTGGGTTCGTGAGTATGAAAACCTTCGTCCTCGGGCGAAGGTTTTTTTTATTATGTGGCGGCTTTATTT
TATCTCATCCGCCCCCAGAT

Product: trigger factor

Products: peptidylproline (w =0) [C]

Alternate protein names: TF

Number of amino acids: Translated: 433; Mature: 433

Protein sequence:

>433_residues
MQVSVETTEGLGRRVNITVTADSIEKAVESELRNVAKKARIDGFRKGKVPMNIVAQRYGASVRQDVLGDLMQRNFVDAII
KEKLNPVGAPKYVPGEYKLGEDFAYAVEFEVYPEIELKGLDTIVVEKPQVDVNDADVDAMIETLRKQQASWSVKEGEVGA
EDRATIDFTGSIDGEAFEGGKATDFVLAMGQGRMIPGFEDGILGHKAGEEFVIDVTFPEDYHAENLKGKAAKFDIVLKKV
EERELPEMTAEFIKRFGVADGSLDGLKAEVRKNMTRELKNAVRNRIKAQVLDGLVLANEIDVPAALVDGEIDVLRRQAAQ
RFGGNEQQAMELPRELFEEQAKRRVIVGLLLGEVISKSELKADDARVNALIEEMASAYEDPQEVIEFYSKNKELMNNMRN
MALEEQAVEALLSQAQVSEKATTFNELMNQPQA

Sequences:

>Translated_433_residues
MQVSVETTEGLGRRVNITVTADSIEKAVESELRNVAKKARIDGFRKGKVPMNIVAQRYGASVRQDVLGDLMQRNFVDAII
KEKLNPVGAPKYVPGEYKLGEDFAYAVEFEVYPEIELKGLDTIVVEKPQVDVNDADVDAMIETLRKQQASWSVKEGEVGA
EDRATIDFTGSIDGEAFEGGKATDFVLAMGQGRMIPGFEDGILGHKAGEEFVIDVTFPEDYHAENLKGKAAKFDIVLKKV
EERELPEMTAEFIKRFGVADGSLDGLKAEVRKNMTRELKNAVRNRIKAQVLDGLVLANEIDVPAALVDGEIDVLRRQAAQ
RFGGNEQQAMELPRELFEEQAKRRVIVGLLLGEVISKSELKADDARVNALIEEMASAYEDPQEVIEFYSKNKELMNNMRN
MALEEQAVEALLSQAQVSEKATTFNELMNQPQA
>Mature_433_residues
MQVSVETTEGLGRRVNITVTADSIEKAVESELRNVAKKARIDGFRKGKVPMNIVAQRYGASVRQDVLGDLMQRNFVDAII
KEKLNPVGAPKYVPGEYKLGEDFAYAVEFEVYPEIELKGLDTIVVEKPQVDVNDADVDAMIETLRKQQASWSVKEGEVGA
EDRATIDFTGSIDGEAFEGGKATDFVLAMGQGRMIPGFEDGILGHKAGEEFVIDVTFPEDYHAENLKGKAAKFDIVLKKV
EERELPEMTAEFIKRFGVADGSLDGLKAEVRKNMTRELKNAVRNRIKAQVLDGLVLANEIDVPAALVDGEIDVLRRQAAQ
RFGGNEQQAMELPRELFEEQAKRRVIVGLLLGEVISKSELKADDARVNALIEEMASAYEDPQEVIEFYSKNKELMNNMRN
MALEEQAVEALLSQAQVSEKATTFNELMNQPQA

Specific function: Involved in protein export. Acts as a chaperone by maintaining the newly synthesized protein in an open conformation

COG id: COG0544

COG function: function code O; FKBP-type peptidyl-prolyl cis-trans isomerase (trigger factor)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PPIase FKBP-type domain

Homologues:

Organism=Escherichia coli, GI1786640, Length=430, Percent_Identity=82.093023255814, Blast_Score=728, Evalue=0.0,

Paralogues:

None

Copy number: 1070 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 500 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 340 Molecules/Cell In: Growth-Phase, Mini

Swissprot (AC and ID): TIG_EDWI9 (C5BCJ5)

Other databases:

- EMBL:   CP001600
- RefSeq:   YP_002932524.1
- GeneID:   7960663
- GenomeReviews:   CP001600_GR
- KEGG:   eic:NT01EI_1077
- OMA:   RFIPGFE
- ProtClustDB:   PRK01490
- HAMAP:   MF_00303
- InterPro:   IPR001179
- InterPro:   IPR005215
- InterPro:   IPR008880
- InterPro:   IPR008881
- Gene3D:   G3DSA:1.10.3120.10
- Gene3D:   G3DSA:3.30.70.1050
- PIRSF:   PIRSF003095
- TIGRFAMs:   TIGR00115

Pfam domain/function: PF00254 FKBP_C; PF05698 Trigger_C; PF05697 Trigger_N; SSF109998 Trigger_fac_C_bac; SSF102735 Trigger_fac_ribosome-bd_bac

EC number: NA

Molecular weight: Translated: 48074; Mature: 48074

Theoretical pI: Translated: 4.48; Mature: 4.48

Prosite motif: PS50059 FKBP_PPIASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQVSVETTEGLGRRVNITVTADSIEKAVESELRNVAKKARIDGFRKGKVPMNIVAQRYGA
CEEEEECHHCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCC
SVRQDVLGDLMQRNFVDAIIKEKLNPVGAPKYVPGEYKLGEDFAYAVEFEVYPEIELKGL
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEEEECCCEEECCC
DTIVVEKPQVDVNDADVDAMIETLRKQQASWSVKEGEVGAEDRATIDFTGSIDGEAFEGG
CEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCCCCCCCC
KATDFVLAMGQGRMIPGFEDGILGHKAGEEFVIDVTFPEDYHAENLKGKAAKFDIVLKKV
CCCEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHH
EERELPEMTAEFIKRFGVADGSLDGLKAEVRKNMTRELKNAVRNRIKAQVLDGLVLANEI
HHHCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
DVPAALVDGEIDVLRRQAAQRFGGNEQQAMELPRELFEEQAKRRVIVGLLLGEVISKSEL
CCCHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
KADDARVNALIEEMASAYEDPQEVIEFYSKNKELMNNMRNMALEEQAVEALLSQAQVSEK
CCHHHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ATTFNELMNQPQA
HHHHHHHHCCCCC
>Mature Secondary Structure
MQVSVETTEGLGRRVNITVTADSIEKAVESELRNVAKKARIDGFRKGKVPMNIVAQRYGA
CEEEEECHHCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCC
SVRQDVLGDLMQRNFVDAIIKEKLNPVGAPKYVPGEYKLGEDFAYAVEFEVYPEIELKGL
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEEEECCCEEECCC
DTIVVEKPQVDVNDADVDAMIETLRKQQASWSVKEGEVGAEDRATIDFTGSIDGEAFEGG
CEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCCCCCCCC
KATDFVLAMGQGRMIPGFEDGILGHKAGEEFVIDVTFPEDYHAENLKGKAAKFDIVLKKV
CCCEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHH
EERELPEMTAEFIKRFGVADGSLDGLKAEVRKNMTRELKNAVRNRIKAQVLDGLVLANEI
HHHCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
DVPAALVDGEIDVLRRQAAQRFGGNEQQAMELPRELFEEQAKRRVIVGLLLGEVISKSEL
CCCHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
KADDARVNALIEEMASAYEDPQEVIEFYSKNKELMNNMRNMALEEQAVEALLSQAQVSEK
CCHHHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ATTFNELMNQPQA
HHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: peptidylproline (w =180) [C]

Specific reaction: peptidylproline (w =180) = peptidylproline (w =0) [C]

General reaction: Isomerases; cis-trans-Isomerases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA