Click here to switch to the map view.

The map label for this gene is adhE [H]

Identifier: 238917964

GI number: 238917964

Start: 2125960

End: 2128575

Strand: Direct

Name: adhE [H]

Synonym: EUBELI_02054

Alternate gene names: 238917964

Gene position: 2125960-2128575 (Clockwise)

Preceding gene: 238917957

Following gene: 238917965

Centisome position: 99.15

GC content: 40.33

Gene sequence:

>2616_bases
ATGTCAAAGAATTTAACTGAGAATTACCCAATTGTTGACACAGTCGAAGCTCTTGAAGAAAGACTTGCAGGTGTACGTGA
GGCACAGCGCATCTTTGCAACCTATACACAGGAGCAGGTAGATAAGATATTTACTGCTGCTGCACTTGCTGCCAATAAAG
CAAGAATCCCTCTTGCAAAGCTTGCAGTTGAAGAAACTGGTATGGGAATTGTAGAAGATAAGGTTATCAAGAATCATTAT
GCTTCAGAATACATTTATAATGCTTACAGAAATACTAAGACATGTGGTGTTATTGAAGAGGATAAGGCATATGGAATTAA
AAAAGTTGCTGAACCTATTGGCGTTGTTGCAGCCGTAATCCCTACAACTAATCCAACTTCAACAGCTATTTTCAAAACTC
TTATTTCACTTAAGACTAGAAATGGTATTATCATAAGCCCACATCCACGAGCTAAGAAATCAACAATTGCAGCGGCTAAG
ATTGTTCTTGAAGCTGCTGTTGCAGCAGGTGCCCCAGCGGGAATCATTGGCTGGATAGATGTTCCTTCACTTGAACTTAC
AGATACACTTATGAAGGAAGCTGACATTATCCTGGCTACCGGAGGACCTGGAATGGTTAAGGCTGCTTACTCTTCTGGAA
AGCCTGCACTTGGTGTAGGTGCCGGTAATACTCCTGCTATCATTGATGAATCAGCAGATGTAATACTTGCTGTTAACTCA
ATTATTCATTCTAAGACATTTGACAATGGTATGATATGTGCTTCTGAACAGTCTGTAATTGTTGACAAGAAGGTTTACAA
GGCTGTTAAAGAAGAATTTGCATACAGAGGCTGTTACTTCCTTAATAAGAGCGAGACAGAAAAGGTAAGAAAGACCATTA
TTATCAATGGTGCCCTTAATGCAAAGATTGTTGGTCAGAAGGCTCACACTATCGCTGCTTTAGCAGGTGTTACTGTTCCT
GAAGAAACTAAGATTCTTATCGGTGAGGTAACATCTGTAGACCTTTCAGAAGAATTTGCACATGAAAAGTTATCACCTGT
TCTTGCAATGTATAAGGCAGAGGATTTTGAAGATGCTCTTTCAAAAGCTGAACACCTTATTGCTGATGGTGGATTTGGTC
ATACATCTTCTCTTTATATTAATGTTGAAACACAGGCAGACAAGATTGCTGAATTCTCTGAAAGAATGAAGACATGCCGC
TGCCTTATCAATACACCTTCATCTCACGGTGGAATCGGTGACCTCTACAACTTTAAGATGGCTCCTTCTCTTACACTTGG
ATGCGGCTCATGGGGCGGCAACTCAGTATCTGAGAACGTTGGAGTAAAACATCTGCTTAATGTAAAGACTGTCGCTGAGA
GGAGAGAGAATATGCTTTGGTTTAGAGCACCTGAGAAGGTTTACTTTAAGAAGGGCTGCTTACCTGTTGCCCTTGATGAA
CTTGGAACTGTAATGGGTAAGAAGAAAGCATTTATCGTTACTGACCAGTTCCTTTATAAGAATGGTTATACCAAATGTGT
CACAGATAAGTTAGATTCTTTAGGAATTATGCATACTACTTTCTATAATGTAGCACCAGATCCAACACTTGCATGCGCTA
AAGAAGGCACCGCAGCCATGAGACTTTTTGAACCTGATGTAATCATTGCAATCGGTGGTGGTTCTGCAATGGATGCTGCC
AAGATTATGTGGGTTATGTATGAACATCCTGAGGCAGACTTCCTTGATATGGCTATGCGTTTCATGGATATCAGAAAGAG
AATATACACATTCCCTAAGATGGGCGAGAAAGCTTACTTTGTCGCTATCTCAACATCTTCTGGAACAGGTTCAGAGGTAA
CTCCTTTTGCAGTTATTACTGATGAAACAACCGGTCAGAAATATCCACTTGCTGACTATGAATTACTTCCTAAGATGGCT
ATTATCGATGCTGACATGCATATGAATCAGCCTAAGGGACTTACAGCAGCTTCTGGTATTGATGCACTTACACATGCACT
TGAAGCATACGCTTCCATTATGGCAACAGAATATACAGATGGTCTTGCATTACAGGCTATGAAGAATATATTTGAATATC
TTCCAGCAGCTTATGAATTAGGTGCACATGATGCAGTTGCAAGAGAAAAGATGGCAACAGCTTCTACTATGGCTGGTATG
GCTTTTGCTAATGCTTTCCTTGGAGTGTGCCACTCACTTGCACATAAACTTGGTGCATATCACCACCTTCCACACGGAAT
TGCTAATGCCCTTCTTATCACTGATGTAATGAGATTCAATGCTGCAGAGGTTCCAACAAAAATGGGAACATTCTCACAGT
ATCAGTATCCACATTGCAAAGCACGTTATGTTGAGTGTGCTGACTTCCTCGGAATTCAGGGTAAAGATGATGATGAGAAA
TTTGAAAACCTCATCAAGGCTATTGAAGAATTAAAGGCAAAGGTAGGAATTAAGAAAACTATTGCAGATTATGGTGTTAA
AGAGGAAGACTTCCTTGCAACTCTTGATGAGATGACAGAAGCCGCATTTGATGACCAGTGTACTGGTGCTAACCCAAGAT
ATCCTCTTATGAGTGAAATGAAAGCCATGTACTTAAAGGCTTACTATGGTAAATAA

Upstream 100 bases:

>100_bases
ATTACAATGAATATTGACTTATAATTATATTAGTGTTATATTGACATTGTTAAATTATTATCAATCAATATTACACGAAT
CAATTTATGGAGGAAATGTT

Downstream 100 bases:

>100_bases
TCATCATTATTATTTTAAGAAAGGAAGACATGTCGTATGAAAAAATATTTAGCAAAATCCAAGTTAAAATCAGTATATGT
AGAGAACAACAAAGCGATTA

Product: bifunctional acetaldehyde-CoA/alcohol dehydrogenase

Products: NA

Alternate protein names: Alcohol dehydrogenase; ADH; Acetaldehyde dehydrogenase [acetylating]; ACDH; Pyruvate-formate-lyase deactivase; PFL deactivase [H]

Number of amino acids: Translated: 871; Mature: 870

Protein sequence:

>871_residues
MSKNLTENYPIVDTVEALEERLAGVREAQRIFATYTQEQVDKIFTAAALAANKARIPLAKLAVEETGMGIVEDKVIKNHY
ASEYIYNAYRNTKTCGVIEEDKAYGIKKVAEPIGVVAAVIPTTNPTSTAIFKTLISLKTRNGIIISPHPRAKKSTIAAAK
IVLEAAVAAGAPAGIIGWIDVPSLELTDTLMKEADIILATGGPGMVKAAYSSGKPALGVGAGNTPAIIDESADVILAVNS
IIHSKTFDNGMICASEQSVIVDKKVYKAVKEEFAYRGCYFLNKSETEKVRKTIIINGALNAKIVGQKAHTIAALAGVTVP
EETKILIGEVTSVDLSEEFAHEKLSPVLAMYKAEDFEDALSKAEHLIADGGFGHTSSLYINVETQADKIAEFSERMKTCR
CLINTPSSHGGIGDLYNFKMAPSLTLGCGSWGGNSVSENVGVKHLLNVKTVAERRENMLWFRAPEKVYFKKGCLPVALDE
LGTVMGKKKAFIVTDQFLYKNGYTKCVTDKLDSLGIMHTTFYNVAPDPTLACAKEGTAAMRLFEPDVIIAIGGGSAMDAA
KIMWVMYEHPEADFLDMAMRFMDIRKRIYTFPKMGEKAYFVAISTSSGTGSEVTPFAVITDETTGQKYPLADYELLPKMA
IIDADMHMNQPKGLTAASGIDALTHALEAYASIMATEYTDGLALQAMKNIFEYLPAAYELGAHDAVAREKMATASTMAGM
AFANAFLGVCHSLAHKLGAYHHLPHGIANALLITDVMRFNAAEVPTKMGTFSQYQYPHCKARYVECADFLGIQGKDDDEK
FENLIKAIEELKAKVGIKKTIADYGVKEEDFLATLDEMTEAAFDDQCTGANPRYPLMSEMKAMYLKAYYGK

Sequences:

>Translated_871_residues
MSKNLTENYPIVDTVEALEERLAGVREAQRIFATYTQEQVDKIFTAAALAANKARIPLAKLAVEETGMGIVEDKVIKNHY
ASEYIYNAYRNTKTCGVIEEDKAYGIKKVAEPIGVVAAVIPTTNPTSTAIFKTLISLKTRNGIIISPHPRAKKSTIAAAK
IVLEAAVAAGAPAGIIGWIDVPSLELTDTLMKEADIILATGGPGMVKAAYSSGKPALGVGAGNTPAIIDESADVILAVNS
IIHSKTFDNGMICASEQSVIVDKKVYKAVKEEFAYRGCYFLNKSETEKVRKTIIINGALNAKIVGQKAHTIAALAGVTVP
EETKILIGEVTSVDLSEEFAHEKLSPVLAMYKAEDFEDALSKAEHLIADGGFGHTSSLYINVETQADKIAEFSERMKTCR
CLINTPSSHGGIGDLYNFKMAPSLTLGCGSWGGNSVSENVGVKHLLNVKTVAERRENMLWFRAPEKVYFKKGCLPVALDE
LGTVMGKKKAFIVTDQFLYKNGYTKCVTDKLDSLGIMHTTFYNVAPDPTLACAKEGTAAMRLFEPDVIIAIGGGSAMDAA
KIMWVMYEHPEADFLDMAMRFMDIRKRIYTFPKMGEKAYFVAISTSSGTGSEVTPFAVITDETTGQKYPLADYELLPKMA
IIDADMHMNQPKGLTAASGIDALTHALEAYASIMATEYTDGLALQAMKNIFEYLPAAYELGAHDAVAREKMATASTMAGM
AFANAFLGVCHSLAHKLGAYHHLPHGIANALLITDVMRFNAAEVPTKMGTFSQYQYPHCKARYVECADFLGIQGKDDDEK
FENLIKAIEELKAKVGIKKTIADYGVKEEDFLATLDEMTEAAFDDQCTGANPRYPLMSEMKAMYLKAYYGK
>Mature_870_residues
SKNLTENYPIVDTVEALEERLAGVREAQRIFATYTQEQVDKIFTAAALAANKARIPLAKLAVEETGMGIVEDKVIKNHYA
SEYIYNAYRNTKTCGVIEEDKAYGIKKVAEPIGVVAAVIPTTNPTSTAIFKTLISLKTRNGIIISPHPRAKKSTIAAAKI
VLEAAVAAGAPAGIIGWIDVPSLELTDTLMKEADIILATGGPGMVKAAYSSGKPALGVGAGNTPAIIDESADVILAVNSI
IHSKTFDNGMICASEQSVIVDKKVYKAVKEEFAYRGCYFLNKSETEKVRKTIIINGALNAKIVGQKAHTIAALAGVTVPE
ETKILIGEVTSVDLSEEFAHEKLSPVLAMYKAEDFEDALSKAEHLIADGGFGHTSSLYINVETQADKIAEFSERMKTCRC
LINTPSSHGGIGDLYNFKMAPSLTLGCGSWGGNSVSENVGVKHLLNVKTVAERRENMLWFRAPEKVYFKKGCLPVALDEL
GTVMGKKKAFIVTDQFLYKNGYTKCVTDKLDSLGIMHTTFYNVAPDPTLACAKEGTAAMRLFEPDVIIAIGGGSAMDAAK
IMWVMYEHPEADFLDMAMRFMDIRKRIYTFPKMGEKAYFVAISTSSGTGSEVTPFAVITDETTGQKYPLADYELLPKMAI
IDADMHMNQPKGLTAASGIDALTHALEAYASIMATEYTDGLALQAMKNIFEYLPAAYELGAHDAVAREKMATASTMAGMA
FANAFLGVCHSLAHKLGAYHHLPHGIANALLITDVMRFNAAEVPTKMGTFSQYQYPHCKARYVECADFLGIQGKDDDEKF
ENLIKAIEELKAKVGIKKTIADYGVKEEDFLATLDEMTEAAFDDQCTGANPRYPLMSEMKAMYLKAYYGK

Specific function: This enzyme has three activities:ADH, ACDH, and PFL- deactivase. In aerobic conditions it acts as a hydrogen peroxide scavenger. The PFL deactivase activity catalyzes the quenching of the pyruvate-formate-lyase catalyst in an iron, NAD, and CoA dependent

COG id: COG1012

COG function: function code C; NAD-dependent aldehyde dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the iron- containing alcohol dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI133922590, Length=339, Percent_Identity=27.4336283185841, Blast_Score=84, Evalue=5e-16,
Organism=Escherichia coli, GI1787493, Length=864, Percent_Identity=63.4259259259259, Blast_Score=1112, Evalue=0.0,
Organism=Escherichia coli, GI1789163, Length=406, Percent_Identity=35.7142857142857, Blast_Score=228, Evalue=1e-60,
Organism=Escherichia coli, GI87082107, Length=382, Percent_Identity=36.6492146596859, Blast_Score=224, Evalue=2e-59,
Organism=Escherichia coli, GI48994951, Length=384, Percent_Identity=37.2395833333333, Blast_Score=211, Evalue=2e-55,
Organism=Escherichia coli, GI1788797, Length=332, Percent_Identity=33.433734939759, Blast_Score=152, Evalue=1e-37,
Organism=Escherichia coli, GI1787684, Length=356, Percent_Identity=26.9662921348315, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI1789386, Length=344, Percent_Identity=24.7093023255814, Blast_Score=70, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI17537053, Length=322, Percent_Identity=24.223602484472, Blast_Score=93, Evalue=6e-19,
Organism=Saccharomyces cerevisiae, GI6321181, Length=418, Percent_Identity=32.5358851674641, Blast_Score=181, Evalue=4e-46,
Organism=Drosophila melanogaster, GI24657991, Length=387, Percent_Identity=26.6149870801034, Blast_Score=92, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001670
- InterPro:   IPR018211
- InterPro:   IPR016161
- InterPro:   IPR016163
- InterPro:   IPR016162
- InterPro:   IPR015590
- InterPro:   IPR012079 [H]

Pfam domain/function: PF00171 Aldedh; PF00465 Fe-ADH [H]

EC number: =1.1.1.1; =1.2.1.10 [H]

Molecular weight: Translated: 94669; Mature: 94538

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: PS00913 ADH_IRON_1 ; PS00060 ADH_IRON_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKNLTENYPIVDTVEALEERLAGVREAQRIFATYTQEQVDKIFTAAALAANKARIPLAK
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
LAVEETGMGIVEDKVIKNHYASEYIYNAYRNTKTCGVIEEDKAYGIKKVAEPIGVVAAVI
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCHHCCHHHHHHHHHEEEEEE
PTTNPTSTAIFKTLISLKTRNGIIISPHPRAKKSTIAAAKIVLEAAVAAGAPAGIIGWID
CCCCCCHHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEC
VPSLELTDTLMKEADIILATGGPGMVKAAYSSGKPALGVGAGNTPAIIDESADVILAVNS
CCCCHHHHHHHHCCCEEEECCCCCEEEEECCCCCCEEEECCCCCCCEECCCCCEEEEHHH
IIHSKTFDNGMICASEQSVIVDKKVYKAVKEEFAYRGCYFLNKSETEKVRKTIIINGALN
HHHHCCCCCCEEEECCCCEEHHHHHHHHHHHHHHHCCEEEECCCHHHHHHHEEEEECCCC
AKIVGQKAHTIAALAGVTVPEETKILIGEVTSVDLSEEFAHEKLSPVLAMYKAEDFEDAL
EEEECCHHHHHHHHHCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCHHHHH
SKAEHLIADGGFGHTSSLYINVETQADKIAEFSERMKTCRCLINTPSSHGGIGDLYNFKM
HHHHHHHCCCCCCCCEEEEEEEECCHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCEEE
APSLTLGCGSWGGNSVSENVGVKHLLNVKTVAERRENMLWFRAPEKVYFKKGCLPVALDE
CCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHCEEEEECCCHHHHHCCCCHHHHHH
LGTVMGKKKAFIVTDQFLYKNGYTKCVTDKLDSLGIMHTTFYNVAPDPTLACAKEGTAAM
HHHHHCCCCEEEEEHHHHHHCCCCHHHHHHHHHCCEEEHEEECCCCCCCHHHCCCCCCEE
RLFEPDVIIAIGGGSAMDAAKIMWVMYEHPEADFLDMAMRFMDIRKRIYTFPKMGEKAYF
EEECCCEEEEECCCCCCCHHEEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEE
VAISTSSGTGSEVTPFAVITDETTGQKYPLADYELLPKMAIIDADMHMNQPKGLTAASGI
EEEECCCCCCCCCCCEEEEECCCCCCCCCCCCHHHCCCEEEEECCCCCCCCCCCCHHHHH
DALTHALEAYASIMATEYTDGLALQAMKNIFEYLPAAYELGAHDAVAREKMATASTMAGM
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHHHHHHHHHH
AFANAFLGVCHSLAHKLGAYHHLPHGIANALLITDVMRFNAAEVPTKMGTFSQYQYPHCK
HHHHHHHHHHHHHHHHHCCHHCCCHHHHHHHHHHHHHHCCCHHCCHHCCCCCCCCCCCCH
ARYVECADFLGIQGKDDDEKFENLIKAIEELKAKVGIKKTIADYGVKEEDFLATLDEMTE
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCCCHHHHHHHHHHHH
AAFDDQCTGANPRYPLMSEMKAMYLKAYYGK
HHCCCCCCCCCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SKNLTENYPIVDTVEALEERLAGVREAQRIFATYTQEQVDKIFTAAALAANKARIPLAK
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
LAVEETGMGIVEDKVIKNHYASEYIYNAYRNTKTCGVIEEDKAYGIKKVAEPIGVVAAVI
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCHHCCHHHHHHHHHEEEEEE
PTTNPTSTAIFKTLISLKTRNGIIISPHPRAKKSTIAAAKIVLEAAVAAGAPAGIIGWID
CCCCCCHHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEC
VPSLELTDTLMKEADIILATGGPGMVKAAYSSGKPALGVGAGNTPAIIDESADVILAVNS
CCCCHHHHHHHHCCCEEEECCCCCEEEEECCCCCCEEEECCCCCCCEECCCCCEEEEHHH
IIHSKTFDNGMICASEQSVIVDKKVYKAVKEEFAYRGCYFLNKSETEKVRKTIIINGALN
HHHHCCCCCCEEEECCCCEEHHHHHHHHHHHHHHHCCEEEECCCHHHHHHHEEEEECCCC
AKIVGQKAHTIAALAGVTVPEETKILIGEVTSVDLSEEFAHEKLSPVLAMYKAEDFEDAL
EEEECCHHHHHHHHHCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCHHHHH
SKAEHLIADGGFGHTSSLYINVETQADKIAEFSERMKTCRCLINTPSSHGGIGDLYNFKM
HHHHHHHCCCCCCCCEEEEEEEECCHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCEEE
APSLTLGCGSWGGNSVSENVGVKHLLNVKTVAERRENMLWFRAPEKVYFKKGCLPVALDE
CCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHCEEEEECCCHHHHHCCCCHHHHHH
LGTVMGKKKAFIVTDQFLYKNGYTKCVTDKLDSLGIMHTTFYNVAPDPTLACAKEGTAAM
HHHHHCCCCEEEEEHHHHHHCCCCHHHHHHHHHCCEEEHEEECCCCCCCHHHCCCCCCEE
RLFEPDVIIAIGGGSAMDAAKIMWVMYEHPEADFLDMAMRFMDIRKRIYTFPKMGEKAYF
EEECCCEEEEECCCCCCCHHEEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEE
VAISTSSGTGSEVTPFAVITDETTGQKYPLADYELLPKMAIIDADMHMNQPKGLTAASGI
EEEECCCCCCCCCCCEEEEECCCCCCCCCCCCHHHCCCEEEEECCCCCCCCCCCCHHHHH
DALTHALEAYASIMATEYTDGLALQAMKNIFEYLPAAYELGAHDAVAREKMATASTMAGM
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHHHHHHHHHH
AFANAFLGVCHSLAHKLGAYHHLPHGIANALLITDVMRFNAAEVPTKMGTFSQYQYPHCK
HHHHHHHHHHHHHHHHHCCHHCCCHHHHHHHHHHHHHHCCCHHCCHHCCCCCCCCCCCCH
ARYVECADFLGIQGKDDDEKFENLIKAIEELKAKVGIKKTIADYGVKEEDFLATLDEMTE
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCCCHHHHHHHHHHHH
AAFDDQCTGANPRYPLMSEMKAMYLKAYYGK
HHCCCCCCCCCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]