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The map label for this gene is dacB [H]

Identifier: 238917952

GI number: 238917952

Start: 2109442

End: 2110836

Strand: Direct

Name: dacB [H]

Synonym: EUBELI_02041

Alternate gene names: 238917952

Gene position: 2109442-2110836 (Clockwise)

Preceding gene: 238917949

Following gene: 238917957

Centisome position: 98.38

GC content: 37.78

Gene sequence:

>1395_bases
ATGAAAAAACATTTTCTTAAAGGATTACTTATAAAGATAACCAGCGCAGTATCTGCCACGGTATTAGCATGTTCTGTATT
TACCACAGATGCACACGCTGCCGCATATAACATAAACTATTCTGAAACAGGCACTGCTAATACTGAAGTTTCATGGCCTG
ATGCCCCGGAAGTTAATTCCAGCAGTTGTATTCTTGTTGATGCTGACACAGGCTCTATTCTATTCGAAAGAAACTCACAT
GAAAAATGTTATCCTGCCAGCACAACCAAAATTCTTACCGGTCTTCTTACCATTGAGAACTGCAATATGGATGATGTAGT
AACATTTTCACAGGCCGCAGCCACTTCCATCAATATATATGAGGATGCTAATTTAGGTTGTAAGGTTGGAGAACAGTTTA
CTGTAAACCAGCTTCTCCATGGACTATTATTATACTCTGCCAATGAAATGGCCTATGCTCTCGGTGAGCATGTAGCCGGC
TCCATAGACGCATTTGTTGATATGATGAATGCCAAGGCAAAAGAACTTGGTGCTTTGAATACACATTTTGCCAATGCAAG
CGGATTATATAATACCAACCACTACACAACAGCATATGATATGGCAATGATTGCAAGAGGCTGTTATAATAATGCTTCTT
TCGTAAGCATTGATTCTACTGCTGACGCATATGTAATTCCTGCAACTAACATGTCTGCACAGCGTACAATCAGACACAGA
CATAAGATGTTGAAAGGCCGTTCTTACTATTATGAATACTGTAAGGGTGGAAAGACAGGATATACTGATGAATCCCAGAA
CACACTTGTAACATTTGCAGAAAAAGATGGCATGCGTCTCATATGTGTTGTATTTAAGGAAGCAACAGATGATTTAAGAT
ATACTGATACAAAAACGCTTTTTGACTGGGGCTTCTCTAATTTTCAGAAATCAACAACTACAGGAAGTCAGCTCGGAGCT
ATGTTTTCCAATGATAATTATTATAATTCAGCTGTTTTCTCGACACTTTCACTAAACTTCGGACTGAATGCTTCATTTAT
CACAATACCTAATAAAAGTTCATTATCAAATGTAACTATGACGGTTGATGATAATTATGACATGACCAATGAAAATGGTG
TTATGACAACACATCTTAAGTTCCTGTACAATGATAATACTGTAGGCACAACTACACTTAATATATCTGCTGCCGGCACG
GACACCGGAAGCCTGCTTCCTTATGTCAGTACAGATGTTTCTTCATCATATAAGCCTAAGACATGCATTAAAATCAATAT
CTGGGTAGTTGTTGGCATCGGTGCTGTTATATTATTCATTATTTATATAATTGAAGAACGTGCCAGAAGAAAACGTCAGA
GAAGACATTACAACAGAAGAAGACGCAGATTATAA

Upstream 100 bases:

>100_bases
TACCATAAATATCACTTTTTGCCAAGCAGGGTTTACTTATGGACAATCCTAAGCTAAAATATAATGGTATATTATATTAA
TCGGAGGAATTATCCATATA

Downstream 100 bases:

>100_bases
CAAACAGAGGATTCGGAGCTATACCCTGAATCCTCTGTTTTCTATATTATCTGTCTGTGTCTCATTACTGATTCCGCCCC
TAGAATATGTAAGACCTGAA

Product: D-alanyl-D-alanine carboxypeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 5*; PBP-5* [H]

Number of amino acids: Translated: 464; Mature: 464

Protein sequence:

>464_residues
MKKHFLKGLLIKITSAVSATVLACSVFTTDAHAAAYNINYSETGTANTEVSWPDAPEVNSSSCILVDADTGSILFERNSH
EKCYPASTTKILTGLLTIENCNMDDVVTFSQAAATSINIYEDANLGCKVGEQFTVNQLLHGLLLYSANEMAYALGEHVAG
SIDAFVDMMNAKAKELGALNTHFANASGLYNTNHYTTAYDMAMIARGCYNNASFVSIDSTADAYVIPATNMSAQRTIRHR
HKMLKGRSYYYEYCKGGKTGYTDESQNTLVTFAEKDGMRLICVVFKEATDDLRYTDTKTLFDWGFSNFQKSTTTGSQLGA
MFSNDNYYNSAVFSTLSLNFGLNASFITIPNKSSLSNVTMTVDDNYDMTNENGVMTTHLKFLYNDNTVGTTTLNISAAGT
DTGSLLPYVSTDVSSSYKPKTCIKINIWVVVGIGAVILFIIYIIEERARRKRQRRHYNRRRRRL

Sequences:

>Translated_464_residues
MKKHFLKGLLIKITSAVSATVLACSVFTTDAHAAAYNINYSETGTANTEVSWPDAPEVNSSSCILVDADTGSILFERNSH
EKCYPASTTKILTGLLTIENCNMDDVVTFSQAAATSINIYEDANLGCKVGEQFTVNQLLHGLLLYSANEMAYALGEHVAG
SIDAFVDMMNAKAKELGALNTHFANASGLYNTNHYTTAYDMAMIARGCYNNASFVSIDSTADAYVIPATNMSAQRTIRHR
HKMLKGRSYYYEYCKGGKTGYTDESQNTLVTFAEKDGMRLICVVFKEATDDLRYTDTKTLFDWGFSNFQKSTTTGSQLGA
MFSNDNYYNSAVFSTLSLNFGLNASFITIPNKSSLSNVTMTVDDNYDMTNENGVMTTHLKFLYNDNTVGTTTLNISAAGT
DTGSLLPYVSTDVSSSYKPKTCIKINIWVVVGIGAVILFIIYIIEERARRKRQRRHYNRRRRRL
>Mature_464_residues
MKKHFLKGLLIKITSAVSATVLACSVFTTDAHAAAYNINYSETGTANTEVSWPDAPEVNSSSCILVDADTGSILFERNSH
EKCYPASTTKILTGLLTIENCNMDDVVTFSQAAATSINIYEDANLGCKVGEQFTVNQLLHGLLLYSANEMAYALGEHVAG
SIDAFVDMMNAKAKELGALNTHFANASGLYNTNHYTTAYDMAMIARGCYNNASFVSIDSTADAYVIPATNMSAQRTIRHR
HKMLKGRSYYYEYCKGGKTGYTDESQNTLVTFAEKDGMRLICVVFKEATDDLRYTDTKTLFDWGFSNFQKSTTTGSQLGA
MFSNDNYYNSAVFSTLSLNFGLNASFITIPNKSSLSNVTMTVDDNYDMTNENGVMTTHLKFLYNDNTVGTTTLNISAAGT
DTGSLLPYVSTDVSSSYKPKTCIKINIWVVVGIGAVILFIIYIIEERARRKRQRRHYNRRRRRL

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors. Required specifically for the synthesis of the spore form of peptidoglycan (cortex) [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Forespore outer membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1786851, Length=326, Percent_Identity=30.3680981595092, Blast_Score=125, Evalue=6e-30,
Organism=Escherichia coli, GI1787062, Length=268, Percent_Identity=32.089552238806, Blast_Score=124, Evalue=2e-29,
Organism=Escherichia coli, GI87082022, Length=304, Percent_Identity=28.9473684210526, Blast_Score=108, Evalue=9e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 51335; Mature: 51335

Theoretical pI: Translated: 7.28; Mature: 7.28

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKHFLKGLLIKITSAVSATVLACSVFTTDAHAAAYNINYSETGTANTEVSWPDAPEVNS
CCCHHHHHHHHHHHHHHHHEEEEEEEEECCCCEEEEECCCCCCCCCCCEECCCCCCCCCC
SSCILVDADTGSILFERNSHEKCYPASTTKILTGLLTIENCNMDDVVTFSQAAATSINIY
CCEEEEECCCCCEEEECCCCCCCCCCCHHHHHEEEEEEECCCCCCEEEEHHHCCEEEEEE
EDANLGCKVGEQFTVNQLLHGLLLYSANEMAYALGEHVAGSIDAFVDMMNAKAKELGALN
ECCCCCCCCCCCEEHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCH
THFANASGLYNTNHYTTAYDMAMIARGCYNNASFVSIDSTADAYVIPATNMSAQRTIRHR
HCCCCCCCCCCCCCEEHHHHHHHHHHHCCCCCEEEEECCCCCEEEEECCCCHHHHHHHHH
HKMLKGRSYYYEYCKGGKTGYTDESQNTLVTFAEKDGMRLICVVFKEATDDLRYTDTKTL
HHHHCCCHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCEEEEEEEECCCCCCEECCCCHH
FDWGFSNFQKSTTTGSQLGAMFSNDNYYNSAVFSTLSLNFGLNASFITIPNKSSLSNVTM
HHCCCCCCCCCCCCHHHHHHEECCCCCCCHHEEEEEEEECCCCEEEEECCCCCCCCEEEE
TVDDNYDMTNENGVMTTHLKFLYNDNTVGTTTLNISAAGTDTGSLLPYVSTDVSSSYKPK
EECCCCCCCCCCCEEEEEEEEEECCCCEEEEEEEEEECCCCCCCCCCEEECCCCCCCCCC
TCIKINIWVVVGIGAVILFIIYIIEERARRKRQRRHYNRRRRRL
EEEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKKHFLKGLLIKITSAVSATVLACSVFTTDAHAAAYNINYSETGTANTEVSWPDAPEVNS
CCCHHHHHHHHHHHHHHHHEEEEEEEEECCCCEEEEECCCCCCCCCCCEECCCCCCCCCC
SSCILVDADTGSILFERNSHEKCYPASTTKILTGLLTIENCNMDDVVTFSQAAATSINIY
CCEEEEECCCCCEEEECCCCCCCCCCCHHHHHEEEEEEECCCCCCEEEEHHHCCEEEEEE
EDANLGCKVGEQFTVNQLLHGLLLYSANEMAYALGEHVAGSIDAFVDMMNAKAKELGALN
ECCCCCCCCCCCEEHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCH
THFANASGLYNTNHYTTAYDMAMIARGCYNNASFVSIDSTADAYVIPATNMSAQRTIRHR
HCCCCCCCCCCCCCEEHHHHHHHHHHHCCCCCEEEEECCCCCEEEEECCCCHHHHHHHHH
HKMLKGRSYYYEYCKGGKTGYTDESQNTLVTFAEKDGMRLICVVFKEATDDLRYTDTKTL
HHHHCCCHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCEEEEEEEECCCCCCEECCCCHH
FDWGFSNFQKSTTTGSQLGAMFSNDNYYNSAVFSTLSLNFGLNASFITIPNKSSLSNVTM
HHCCCCCCCCCCCCHHHHHHEECCCCCCCHHEEEEEEEECCCCEEEEECCCCCCCCEEEE
TVDDNYDMTNENGVMTTHLKFLYNDNTVGTTTLNISAAGTDTGSLLPYVSTDVSSSYKPK
EECCCCCCCCCCCEEEEEEEEEECCCCEEEEEEEEEECCCCCCCCCCEEECCCCCCCCCC
TCIKINIWVVVGIGAVILFIIYIIEERARRKRQRRHYNRRRRRL
EEEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1548223; 7934829; 9384377 [H]