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The map label for this gene is trmB [H]

Identifier: 238917951

GI number: 238917951

Start: 2108607

End: 2109251

Strand: Reverse

Name: trmB [H]

Synonym: EUBELI_02040

Alternate gene names: 238917951

Gene position: 2109251-2108607 (Counterclockwise)

Preceding gene: 238917953

Following gene: 238917950

Centisome position: 98.37

GC content: 38.6

Gene sequence:

>645_bases
ATGAGACTTAGAAATGTACCCGGAGCAAGGGAAGTAATGATAGAGAATGAATATGTGTTTACAGAGCCAGAGGGGATGAA
AGGCACATGGTCACAGGTTTTCGGTAACGATAATCCAATCCGTATTGAGATTGGTATGGGTAAAGGCACATTTATAACAA
CACTGGCAGCGAATAATCCTGATATTAATTATGTGGGTATTGAAAAGTATTCAAGCGTGCTTTTAAGAGCTGTAGAGAAA
CAGGACGAACTTCAGCTTCCTAATTTAAGATTCATCAGAATGGATGCAGAGGCTATATGTGAGGTGTTTGGAGAGGGAGA
GGTAGACAGGATATACCTTAATTTTTCAGATCCATGGCCAAAAGACAGACATGCGAAGAGAAGACTTACCTCAAGACAGT
TCATGGCGAGATATGATGTTATATTAAAGCCGGACGGACAGGTGGAATTCAAAACAGATAATAAAGATTTATTTGATTTT
TCATTAGAAGAGATTAAAGAGGCAGGCTGGGAGCTTCCTGTTGTAACATTTGACTTACATAATGACTCTGTACTTAACGA
GGGTAATGTAATGACAGAGTATGAGACAAGATTTTCACAGATGGGCAATCCTATATGTAAACTTGTTGCGGTAAGAAATC
ATTAA

Upstream 100 bases:

>100_bases
ATTTATGGTAAATAATATATAGAAAACTCAGATTTCGGCTATGTCTGTGTGCGGCATATGCTTTCAGGGAGATTGAGTAA
ATAAAAGATAGGAGTAACGT

Downstream 100 bases:

>100_bases
TAATAAATGCATATACTATATAAAGGCTTTGGGAGAAATGTTGTGAAGAATAAAGCATTTGCAAGGAAAGTATCAGAAGT
GATGTATGTGCATAAAGGTG

Product: tRNA (guanine-N7-)-methyltransferase

Products: NA

Alternate protein names: tRNA(m7G46)-methyltransferase [H]

Number of amino acids: Translated: 214; Mature: 214

Protein sequence:

>214_residues
MRLRNVPGAREVMIENEYVFTEPEGMKGTWSQVFGNDNPIRIEIGMGKGTFITTLAANNPDINYVGIEKYSSVLLRAVEK
QDELQLPNLRFIRMDAEAICEVFGEGEVDRIYLNFSDPWPKDRHAKRRLTSRQFMARYDVILKPDGQVEFKTDNKDLFDF
SLEEIKEAGWELPVVTFDLHNDSVLNEGNVMTEYETRFSQMGNPICKLVAVRNH

Sequences:

>Translated_214_residues
MRLRNVPGAREVMIENEYVFTEPEGMKGTWSQVFGNDNPIRIEIGMGKGTFITTLAANNPDINYVGIEKYSSVLLRAVEK
QDELQLPNLRFIRMDAEAICEVFGEGEVDRIYLNFSDPWPKDRHAKRRLTSRQFMARYDVILKPDGQVEFKTDNKDLFDF
SLEEIKEAGWELPVVTFDLHNDSVLNEGNVMTEYETRFSQMGNPICKLVAVRNH
>Mature_214_residues
MRLRNVPGAREVMIENEYVFTEPEGMKGTWSQVFGNDNPIRIEIGMGKGTFITTLAANNPDINYVGIEKYSSVLLRAVEK
QDELQLPNLRFIRMDAEAICEVFGEGEVDRIYLNFSDPWPKDRHAKRRLTSRQFMARYDVILKPDGQVEFKTDNKDLFDF
SLEEIKEAGWELPVVTFDLHNDSVLNEGNVMTEYETRFSQMGNPICKLVAVRNH

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA [H]

COG id: COG0220

COG function: function code R; Predicted S-adenosylmethionine-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. TrmB family [H]

Homologues:

Organism=Escherichia coli, GI1789330, Length=183, Percent_Identity=27.3224043715847, Blast_Score=69, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003358 [H]

Pfam domain/function: PF02390 Methyltransf_4 [H]

EC number: =2.1.1.33 [H]

Molecular weight: Translated: 24700; Mature: 24700

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLRNVPGAREVMIENEYVFTEPEGMKGTWSQVFGNDNPIRIEIGMGKGTFITTLAANNP
CCCCCCCCHHEEEEECCEEEECCCCCCCCHHHHCCCCCCEEEEEECCCCEEEEEEECCCC
DINYVGIEKYSSVLLRAVEKQDELQLPNLRFIRMDAEAICEVFGEGEVDRIYLNFSDPWP
CCEEEEHHHHHHHHHHHHHHHCCCCCCCEEEEEECHHHHHHHHCCCCEEEEEEECCCCCC
KDRHAKRRLTSRQFMARYDVILKPDGQVEFKTDNKDLFDFSLEEIKEAGWELPVVTFDLH
CCHHHHHHHHHHHHHHHEEEEECCCCCEEEEECCCCEECCCHHHHHHCCCCCCEEEEECC
NDSVLNEGNVMTEYETRFSQMGNPICKLVAVRNH
CCCEECCCCEEEHHHHHHHHHCCCEEEEEEECCC
>Mature Secondary Structure
MRLRNVPGAREVMIENEYVFTEPEGMKGTWSQVFGNDNPIRIEIGMGKGTFITTLAANNP
CCCCCCCCHHEEEEECCEEEECCCCCCCCHHHHCCCCCCEEEEEECCCCEEEEEEECCCC
DINYVGIEKYSSVLLRAVEKQDELQLPNLRFIRMDAEAICEVFGEGEVDRIYLNFSDPWP
CCEEEEHHHHHHHHHHHHHHHCCCCCCCEEEEEECHHHHHHHHCCCCEEEEEEECCCCCC
KDRHAKRRLTSRQFMARYDVILKPDGQVEFKTDNKDLFDFSLEEIKEAGWELPVVTFDLH
CCHHHHHHHHHHHHHHHEEEEECCCCCEEEEECCCCEECCCHHHHHHCCCCCCEEEEECC
NDSVLNEGNVMTEYETRFSQMGNPICKLVAVRNH
CCCEECCCCEEEHHHHHHHHHCCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA