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The map label for this gene is mhpE [C]

Identifier: 238917832

GI number: 238917832

Start: 1978084

End: 1979064

Strand: Reverse

Name: mhpE [C]

Synonym: EUBELI_01913

Alternate gene names: 238917832

Gene position: 1979064-1978084 (Counterclockwise)

Preceding gene: 238917833

Following gene: 238917831

Centisome position: 92.3

GC content: 38.84

Gene sequence:

>981_bases
ATGGCAGAAAACAGGGGTAATTTGACTTCTTACAGACCAGACATCAAGGTTATGGACTGTACATTAAGAGATGGAGGTCT
TGTGAATAATTTTGAATTCACTGATGAGTTTGTTAAAGATCTTTATGAAGCTAATGTGGCAGCAGGCGTTGATTATATGG
AATTTGGATATAAGGCTGATAAGGATATATTTGATGAGAAAGAATTCGGAAAGTGGAAGTTCTGTAAGGAAGAAGATATC
AGAGCTATTGTTGGCGAGAATGATACACCACTTAAGATATCAGTTATGGCTGATGTCGGAAGAACTAATATGAAGAGAGA
TATTCCACCTAAGTCAGAGTCAGTTGTTGATATGGTGAGAGTTGCAACATATATTAATACCATTCCGGCAGCTATTGAGA
TGGCTAACTACTGTTCAGATATGGGATATGAGGTTACAGTCAATATTATGGCGATATCGACAGCAGGTGAGAATGAGTTA
GACCTTGCATTAGAGCTTCTTGCATCACAGAGCAAGGCACAGTACATATACCTTGTTGACAGCTATGGTTCATTATACCC
GGAGCAGGTAAGAAGACTTGCTGACAAATACATTAAGATAGCAGAGAAGTATGGAAAAAGTGTTGGTATACATGCACATA
ACAACCAGCAGCTTGCATTTGCCAATACAATTGAGGCATGCTCAATCGGCGTAAGCCTTCTTGATGCAACAGTAAGCGGA
ATGGGAAGAGGTGCAGGTAACTGCTATTCAGAATTATTATTAGGGTTCTTAAGAAATCCTAAGTTTAATATTGTTCCAGT
GCTTAAGTTTATTGAAAAGCATATGGTTCCACTTAAAGCATCAGGTGTTGTGTGGGGATGTGATGTACAGTACATGCTTA
CCGGTCAGACTAACCAGCATCCAAGAACAGCAATTGCATTTACTAAGGCAGAAAGAACCGATTATGCTAAATATTACACA
GAAATTACCGGAGATGAGTAA

Upstream 100 bases:

>100_bases
TTGATAGATGTGTTATAATTCAAAACAGTTTAACACTTTAAAGCGTAACGGTTTAAAGCGAAACAATTCCAGGAGGAAAT
TTTACAGGAGGAAACAGGTT

Downstream 100 bases:

>100_bases
TGAAATGTTGACATGACATTTTTAAGAGCGTAAAATATGCTCAAAATCGTGTAAAACATTTTTTACATTGGTATCTTTAG
ATGAAAGGAAGTACTAACAA

Product: hypothetical protein

Products: NA

Alternate protein names: HOA; 4-hydroxy-2-keto-pentanoic acid aldolase; 4-hydroxy-2-oxopentanoate aldolase [H]

Number of amino acids: Translated: 326; Mature: 325

Protein sequence:

>326_residues
MAENRGNLTSYRPDIKVMDCTLRDGGLVNNFEFTDEFVKDLYEANVAAGVDYMEFGYKADKDIFDEKEFGKWKFCKEEDI
RAIVGENDTPLKISVMADVGRTNMKRDIPPKSESVVDMVRVATYINTIPAAIEMANYCSDMGYEVTVNIMAISTAGENEL
DLALELLASQSKAQYIYLVDSYGSLYPEQVRRLADKYIKIAEKYGKSVGIHAHNNQQLAFANTIEACSIGVSLLDATVSG
MGRGAGNCYSELLLGFLRNPKFNIVPVLKFIEKHMVPLKASGVVWGCDVQYMLTGQTNQHPRTAIAFTKAERTDYAKYYT
EITGDE

Sequences:

>Translated_326_residues
MAENRGNLTSYRPDIKVMDCTLRDGGLVNNFEFTDEFVKDLYEANVAAGVDYMEFGYKADKDIFDEKEFGKWKFCKEEDI
RAIVGENDTPLKISVMADVGRTNMKRDIPPKSESVVDMVRVATYINTIPAAIEMANYCSDMGYEVTVNIMAISTAGENEL
DLALELLASQSKAQYIYLVDSYGSLYPEQVRRLADKYIKIAEKYGKSVGIHAHNNQQLAFANTIEACSIGVSLLDATVSG
MGRGAGNCYSELLLGFLRNPKFNIVPVLKFIEKHMVPLKASGVVWGCDVQYMLTGQTNQHPRTAIAFTKAERTDYAKYYT
EITGDE
>Mature_325_residues
AENRGNLTSYRPDIKVMDCTLRDGGLVNNFEFTDEFVKDLYEANVAAGVDYMEFGYKADKDIFDEKEFGKWKFCKEEDIR
AIVGENDTPLKISVMADVGRTNMKRDIPPKSESVVDMVRVATYINTIPAAIEMANYCSDMGYEVTVNIMAISTAGENELD
LALELLASQSKAQYIYLVDSYGSLYPEQVRRLADKYIKIAEKYGKSVGIHAHNNQQLAFANTIEACSIGVSLLDATVSGM
GRGAGNCYSELLLGFLRNPKFNIVPVLKFIEKHMVPLKASGVVWGCDVQYMLTGQTNQHPRTAIAFTKAERTDYAKYYTE
ITGDE

Specific function: Catalyzes the retro-aldol cleavage of 4-hydroxy-2- oxopentanoate to pyruvate and acetaldehyde. Is involved in the meta-cleavage pathway for the degradation of aromatic compounds [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 4-hydroxy-2-oxovalerate aldolase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017629
- InterPro:   IPR013785
- InterPro:   IPR012425
- InterPro:   IPR000891
- ProDom:   PD005364 [H]

Pfam domain/function: PF07836 DmpG_comm; PF00682 HMGL-like [H]

EC number: =4.1.3.39 [H]

Molecular weight: Translated: 36411; Mature: 36280

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAENRGNLTSYRPDIKVMDCTLRDGGLVNNFEFTDEFVKDLYEANVAAGVDYMEFGYKAD
CCCCCCCCCCCCCCCEEEEEEECCCCEECCCHHHHHHHHHHHHHHHHCCCHHHHCCCCCC
KDIFDEKEFGKWKFCKEEDIRAIVGENDTPLKISVMADVGRTNMKRDIPPKSESVVDMVR
CCCCCCCCCCCCCCCCCCCCEEEECCCCCCEEEEEEECCCCCCCCCCCCCCCHHHHHHHH
VATYINTIPAAIEMANYCSDMGYEVTVNIMAISTAGENELDLALELLASQSKAQYIYLVD
HHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEECCCCCHHHHHHHHHHCCCCCEEEEEEC
SYGSLYPEQVRRLADKYIKIAEKYGKSVGIHAHNNQQLAFANTIEACSIGVSLLDATVSG
CCCCCCHHHHHHHHHHHHHHHHHHCCEEEEEECCCCEEEEHHHHHHHHHHHHHHHHHHHH
MGRGAGNCYSELLLGFLRNPKFNIVPVLKFIEKHMVPLKASGVVWGCDVQYMLTGQTNQH
CCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCEECCCEEEECCEEEEEECCCCCC
PRTAIAFTKAERTDYAKYYTEITGDE
CCEEEEEEHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
AENRGNLTSYRPDIKVMDCTLRDGGLVNNFEFTDEFVKDLYEANVAAGVDYMEFGYKAD
CCCCCCCCCCCCCCEEEEEEECCCCEECCCHHHHHHHHHHHHHHHHCCCHHHHCCCCCC
KDIFDEKEFGKWKFCKEEDIRAIVGENDTPLKISVMADVGRTNMKRDIPPKSESVVDMVR
CCCCCCCCCCCCCCCCCCCCEEEECCCCCCEEEEEEECCCCCCCCCCCCCCCHHHHHHHH
VATYINTIPAAIEMANYCSDMGYEVTVNIMAISTAGENELDLALELLASQSKAQYIYLVD
HHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEECCCCCHHHHHHHHHHCCCCCEEEEEEC
SYGSLYPEQVRRLADKYIKIAEKYGKSVGIHAHNNQQLAFANTIEACSIGVSLLDATVSG
CCCCCCHHHHHHHHHHHHHHHHHHCCEEEEEECCCCEEEEHHHHHHHHHHHHHHHHHHHH
MGRGAGNCYSELLLGFLRNPKFNIVPVLKFIEKHMVPLKASGVVWGCDVQYMLTGQTNQH
CCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCEECCCEEEECCEEEEEECCCCCC
PRTAIAFTKAERTDYAKYYTEITGDE
CCEEEEEEHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA