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The map label for this gene is ilvE [H]

Identifier: 238917831

GI number: 238917831

Start: 1976959

End: 1977984

Strand: Reverse

Name: ilvE [H]

Synonym: EUBELI_01912

Alternate gene names: 238917831

Gene position: 1977984-1976959 (Counterclockwise)

Preceding gene: 238917832

Following gene: 238917830

Centisome position: 92.25

GC content: 42.59

Gene sequence:

>1026_bases
ATGGAAAAGAAGAATATTGATTGGTCAAGCCTTGGCTTTGGTTACATCAAGACTGACAAGAGATTCGTAGCCAACTATAA
AGATGGCGCATGGGATAACGGAACACTTACAGAGGATGACAAGGTTGTCATTAACGAGTGTGCAGGTGTCCTTCAGTATG
CACAGACATGTTTTGAAGGTCTTAAGGCTTATACAACAGAGGATGGTCACATTGTTATGTTCCGCCCAGATCTGAATGCA
GCAAGAATGAAAGATTCATGTGAGAGACTTGAGATGCCAGTTTACCCAGAAGATAAGTGGGTAGAGGCAGTTGCTAAGAC
AGTAGAAGCTAATGCAGCATGGGTACCACCTTTTGGTTCAGGTGCAACACTTTATGTCCGTCCATACATGTTCGGAAGCA
ACCCTGTTATCGGAGTTAAGCCTGCTGATGAATATCAGTTCAGAGTACTTACAACTCCAGTAGGTCCTTACTTCAAGGGC
GGTGCCAAGCCTATTACTATCAAGGTTTCAGATTTTGATAGAGCAGCACCACACGGAACAGGACATATCAAGGCTGGACT
TAACTATGCTATGAGCTTACATGCAATTATGACAGCACATGAAGAAGGATTTGCAGAGAATATGTACCTTGACCCAGCAA
CAAGAACTAAGGTTGAGGAGACAGGTGGTGCTAACTTCATATTCATCACTAAAGATGGTAAGTTCGTAACACCAAAGTCT
AATTCAATTCTTCCATCAATCACACGCCGTTCATTAATGGTTGTTGCTAAGGAATATCTTGGATTAGAGGTTGAGGAAAG
AGAAGTTCTCTTTGATGAAGTTAAGGATTTTGCAGAGTGTGGTCTTTGCGGTACAGCTGCAGTTATCTCACCTGTTGGAA
AGATTAACGACCATGGTAAGGAAATCTGCTTCCCAAGCGGAATGGAGAAGATGGGTCCTACAATCCAGAAGCTCTATGAT
ACATTAACAGGTATCCAGATGGGCAAGATTGAAGCTCCTAAGGGATGGATTTACACAATCGCTTAA

Upstream 100 bases:

>100_bases
ATGAAATGTTGACATGACATTTTTAAGAGCGTAAAATATGCTCAAAATCGTGTAAAACATTTTTTACATTGGTATCTTTA
GATGAAAGGAAGTACTAACA

Downstream 100 bases:

>100_bases
TTGATATTCTTTAAATGTTTATTCGGTCTGGACAAGTGTTCAGACCGATTTTTTAATTTTCTAAAGTGGATTTTACATTT
AAAATGTGGTAACATACTAA

Product: branched-chain amino acid aminotransferase

Products: NA

Alternate protein names: BCAT [H]

Number of amino acids: Translated: 341; Mature: 341

Protein sequence:

>341_residues
MEKKNIDWSSLGFGYIKTDKRFVANYKDGAWDNGTLTEDDKVVINECAGVLQYAQTCFEGLKAYTTEDGHIVMFRPDLNA
ARMKDSCERLEMPVYPEDKWVEAVAKTVEANAAWVPPFGSGATLYVRPYMFGSNPVIGVKPADEYQFRVLTTPVGPYFKG
GAKPITIKVSDFDRAAPHGTGHIKAGLNYAMSLHAIMTAHEEGFAENMYLDPATRTKVEETGGANFIFITKDGKFVTPKS
NSILPSITRRSLMVVAKEYLGLEVEEREVLFDEVKDFAECGLCGTAAVISPVGKINDHGKEICFPSGMEKMGPTIQKLYD
TLTGIQMGKIEAPKGWIYTIA

Sequences:

>Translated_341_residues
MEKKNIDWSSLGFGYIKTDKRFVANYKDGAWDNGTLTEDDKVVINECAGVLQYAQTCFEGLKAYTTEDGHIVMFRPDLNA
ARMKDSCERLEMPVYPEDKWVEAVAKTVEANAAWVPPFGSGATLYVRPYMFGSNPVIGVKPADEYQFRVLTTPVGPYFKG
GAKPITIKVSDFDRAAPHGTGHIKAGLNYAMSLHAIMTAHEEGFAENMYLDPATRTKVEETGGANFIFITKDGKFVTPKS
NSILPSITRRSLMVVAKEYLGLEVEEREVLFDEVKDFAECGLCGTAAVISPVGKINDHGKEICFPSGMEKMGPTIQKLYD
TLTGIQMGKIEAPKGWIYTIA
>Mature_341_residues
MEKKNIDWSSLGFGYIKTDKRFVANYKDGAWDNGTLTEDDKVVINECAGVLQYAQTCFEGLKAYTTEDGHIVMFRPDLNA
ARMKDSCERLEMPVYPEDKWVEAVAKTVEANAAWVPPFGSGATLYVRPYMFGSNPVIGVKPADEYQFRVLTTPVGPYFKG
GAKPITIKVSDFDRAAPHGTGHIKAGLNYAMSLHAIMTAHEEGFAENMYLDPATRTKVEETGGANFIFITKDGKFVTPKS
NSILPSITRRSLMVVAKEYLGLEVEEREVLFDEVKDFAECGLCGTAAVISPVGKINDHGKEICFPSGMEKMGPTIQKLYD
TLTGIQMGKIEAPKGWIYTIA

Specific function: Acts on leucine, isoleucine and valine [H]

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI296010904, Length=344, Percent_Identity=31.1046511627907, Blast_Score=152, Evalue=3e-37,
Organism=Homo sapiens, GI296010906, Length=344, Percent_Identity=31.1046511627907, Blast_Score=152, Evalue=3e-37,
Organism=Homo sapiens, GI38176287, Length=344, Percent_Identity=31.1046511627907, Blast_Score=152, Evalue=4e-37,
Organism=Homo sapiens, GI50658084, Length=331, Percent_Identity=29.9093655589124, Blast_Score=136, Evalue=3e-32,
Organism=Homo sapiens, GI258614015, Length=284, Percent_Identity=31.6901408450704, Blast_Score=119, Evalue=3e-27,
Organism=Homo sapiens, GI296010902, Length=252, Percent_Identity=31.3492063492063, Blast_Score=108, Evalue=5e-24,
Organism=Homo sapiens, GI296010900, Length=252, Percent_Identity=31.3492063492063, Blast_Score=108, Evalue=6e-24,
Organism=Escherichia coli, GI48994963, Length=317, Percent_Identity=29.9684542586751, Blast_Score=111, Evalue=9e-26,
Organism=Caenorhabditis elegans, GI17568601, Length=360, Percent_Identity=32.2222222222222, Blast_Score=166, Evalue=1e-41,
Organism=Caenorhabditis elegans, GI17565728, Length=356, Percent_Identity=29.7752808988764, Blast_Score=112, Evalue=3e-25,
Organism=Saccharomyces cerevisiae, GI6322608, Length=341, Percent_Identity=31.9648093841642, Blast_Score=167, Evalue=3e-42,
Organism=Saccharomyces cerevisiae, GI6322002, Length=350, Percent_Identity=32.2857142857143, Blast_Score=154, Evalue=1e-38,
Organism=Drosophila melanogaster, GI24641779, Length=338, Percent_Identity=32.5443786982249, Blast_Score=173, Evalue=2e-43,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001544
- InterPro:   IPR018300
- InterPro:   IPR005786 [H]

Pfam domain/function: PF01063 Aminotran_4 [H]

EC number: =2.6.1.42 [H]

Molecular weight: Translated: 37646; Mature: 37646

Theoretical pI: Translated: 5.48; Mature: 5.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKKNIDWSSLGFGYIKTDKRFVANYKDGAWDNGTLTEDDKVVINECAGVLQYAQTCFEG
CCCCCCCHHHCCCCEEECCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
LKAYTTEDGHIVMFRPDLNAARMKDSCERLEMPVYPEDKWVEAVAKTVEANAAWVPPFGS
HHHEECCCCEEEEECCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCEECCCCCC
GATLYVRPYMFGSNPVIGVKPADEYQFRVLTTPVGPYFKGGAKPITIKVSDFDRAAPHGT
CCEEEEEEEEECCCCEEECCCCCCEEEEEEECCCCCCCCCCCCEEEEEECCCCCCCCCCC
GHIKAGLNYAMSLHAIMTAHEEGFAENMYLDPATRTKVEETGGANFIFITKDGKFVTPKS
CEEECCHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCHHHCCCCEEEEEECCCCEECCCC
NSILPSITRRSLMVVAKEYLGLEVEEREVLFDEVKDFAECGLCGTAAVISPVGKINDHGK
CCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCCC
EICFPSGMEKMGPTIQKLYDTLTGIQMGKIEAPKGWIYTIA
EECCCCCHHHHCHHHHHHHHHHHCCEECCEECCCCEEEEEC
>Mature Secondary Structure
MEKKNIDWSSLGFGYIKTDKRFVANYKDGAWDNGTLTEDDKVVINECAGVLQYAQTCFEG
CCCCCCCHHHCCCCEEECCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
LKAYTTEDGHIVMFRPDLNAARMKDSCERLEMPVYPEDKWVEAVAKTVEANAAWVPPFGS
HHHEECCCCEEEEECCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCEECCCCCC
GATLYVRPYMFGSNPVIGVKPADEYQFRVLTTPVGPYFKGGAKPITIKVSDFDRAAPHGT
CCEEEEEEEEECCCCEEECCCCCCEEEEEEECCCCCCCCCCCCEEEEEECCCCCCCCCCC
GHIKAGLNYAMSLHAIMTAHEEGFAENMYLDPATRTKVEETGGANFIFITKDGKFVTPKS
CEEECCHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCHHHCCCCEEEEEECCCCEECCCC
NSILPSITRRSLMVVAKEYLGLEVEEREVLFDEVKDFAECGLCGTAAVISPVGKINDHGK
CCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCCC
EICFPSGMEKMGPTIQKLYDTLTGIQMGKIEAPKGWIYTIA
EECCCCCHHHHCHHHHHHHHHHHCCEECCEECCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]