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The map label for this gene is ydiS [H]

Identifier: 238917712

GI number: 238917712

Start: 1851722

End: 1853605

Strand: Reverse

Name: ydiS [H]

Synonym: EUBELI_01792

Alternate gene names: 238917712

Gene position: 1853605-1851722 (Counterclockwise)

Preceding gene: 238917713

Following gene: 238917711

Centisome position: 86.45

GC content: 34.93

Gene sequence:

>1884_bases
ATGGAGTTTACAGAGGCAATAGAGATAATAAAGAACATAGTTCCGGAGAGTTCAGCAAATGAATATGATGGAATAATGGT
AAAAAGACTGGAAGCATCTAACACATTGGATGAAGGCAGAACAACAAATCAGACCCATATAGCAATATCAGGAGAACAGA
TGAATATGTTCCCTTATCTTATGGCAGATGGCTATTTTAAGTGCGATTATAATGACAGGGATGAGCAGTTAAAAAAATAT
TTTATAACACAGATACCTCTTTATATTTACAAGGATAATGTCCTGTATCTTGCAGATGGTGAAGAGAGCGGTATCAGCTT
TGGTGATGAAAAAAGCAGATGTGTGCGTGCGAGTATAGTGAGAAGTAGAAGAAAAGAGCAGGCAGATCAGGTACAGCTGT
CATTGACTACAATAGATGATCCGGCATTTGTCAGCTTCAGAAGATTATTGCATACAGGAGATTATCTGGTGATTCTGAAG
CATAAAGAAAAGCTGGTTTATGATTGTATAGGCATTAAAGCCAATGATGAAACTCGTGGAGAGTTTAATATTTCAGCACT
TAATAATAAATTTTATAAGCTGCCGACAAATACAAAAGTAGATATAAAGGAACTTATTGAAATTAAAAATGCAGAAATAG
AAAACAAGAAATTCACAGTTGATGAATTGGGGACTATTCTTAAAGAAATGTATGGCAATGCAGAAGATAAAATGCAGGTA
GCATCTATACATATTTTTGGAATAAAATATGGCAAAAATATAATCGAAAATGAATTCAAGGCACCAGATATTATCAAAGC
AGCAGGATTGAATGAGTCATACTCCACAGAATTACAGAAGGCTTTGAATATATATAGGTGTCTCAATAAAAATACATACG
GAATCTCTATTTCTGAGGGTGATAATGTACTGGAAGAAAATAAAGAATCTGTTAAGCGTAAAACAGGAGCAGAAAATATT
CTTCTTTATGGCGTTCCAGGTGCTGGAAAAAGTCATGAAATAAAGACAAAATATTGCGATGATGAAAAATACATGGAGAG
AGTGGTTTTTCATCCGGATTATACATATTCAGATTTTGTGGGACAAATATTACCGAGAGTGGAAAAGGATAGAGATGGTA
ACGATAAGCTGAAATATGTATTTACTCCTGGACCATATACTAAGTTGCTTAAGAAGGCACAAAATGATCCGGGTAACTAC
TATTATCTTGTCATAGAAGAATTAAACAGAGGTAATGCTCCTGCAATATTTGGAGAGGTATTCCAGTTGCTTGACAGAAA
AGATGAAGAGGATTTTCCTCTTGAAGAAGTAGGCGAAAGTGAATATGGAATTTCAAATTATGAGGTTGCAAAGGAAGTCT
ACGGGGATGAAAAACATCAGGTAAGAATACCATCAAATATGTATGTTCTGGCTACGATGAATACAGCAGATCAGAATGTA
TTTACATTGGACACTGCTTTTCAGCGTAGGTGGAATATGAGACAGATAGAGAATAATTTTGATAAGTCTGAACATTCAAA
AGATATAATTGCGGGTACAAAAGTAAGCTGGGGAGCATTTGCTACAGTAATCAATGATATGGTCATTGATATCAATGTTG
ATATGGCAAGCTCTGAGGATAAGCGGCTTGGAACATATTTTGCCAGAAAGAAAGAGCTGGAGGCGGGCAGATTTTCTGAA
AAGGTACTTAAATATTTATGGGATGATGCTTTTAAAATGGACAAGACAGCTATATTTAATGAAAATTGTAAGTCTCTTGA
AGATGTGGTTATTACATATGAGACTGCAACATCAGATAAACTGGCAGCGGTATTGAGAATGAGCGTTTATGAGAAAATGC
TTTCAAAAATGCAACAAAAGAATACAGATAATAATGAAAACTAG

Upstream 100 bases:

>100_bases
ATACGAAGACTTATATGCAGATAGGAAATTCTGTTCCTCCACTTCTGGGAAGAGCGATAGCACATGTGATAAAAAGCTAT
ATGAAAGGTGGTAATGACTG

Downstream 100 bases:

>100_bases
GTGGTGATTTTTTGAGGAAAGTTGATATTCGAAGTAAATGCAGAGTGAATTCTAATCGTGAAATGGATACATTTGTAGGG
TTAAGATGTAATGATGGTGA

Product: hypothetical protein

Products: NA

Alternate protein names: R.BsuM; R.BsuMI; Endonuclease BsuMI component ydiS; Type II restriction enzyme BsuMI component ydiS [H]

Number of amino acids: Translated: 627; Mature: 627

Protein sequence:

>627_residues
MEFTEAIEIIKNIVPESSANEYDGIMVKRLEASNTLDEGRTTNQTHIAISGEQMNMFPYLMADGYFKCDYNDRDEQLKKY
FITQIPLYIYKDNVLYLADGEESGISFGDEKSRCVRASIVRSRRKEQADQVQLSLTTIDDPAFVSFRRLLHTGDYLVILK
HKEKLVYDCIGIKANDETRGEFNISALNNKFYKLPTNTKVDIKELIEIKNAEIENKKFTVDELGTILKEMYGNAEDKMQV
ASIHIFGIKYGKNIIENEFKAPDIIKAAGLNESYSTELQKALNIYRCLNKNTYGISISEGDNVLEENKESVKRKTGAENI
LLYGVPGAGKSHEIKTKYCDDEKYMERVVFHPDYTYSDFVGQILPRVEKDRDGNDKLKYVFTPGPYTKLLKKAQNDPGNY
YYLVIEELNRGNAPAIFGEVFQLLDRKDEEDFPLEEVGESEYGISNYEVAKEVYGDEKHQVRIPSNMYVLATMNTADQNV
FTLDTAFQRRWNMRQIENNFDKSEHSKDIIAGTKVSWGAFATVINDMVIDINVDMASSEDKRLGTYFARKKELEAGRFSE
KVLKYLWDDAFKMDKTAIFNENCKSLEDVVITYETATSDKLAAVLRMSVYEKMLSKMQQKNTDNNEN

Sequences:

>Translated_627_residues
MEFTEAIEIIKNIVPESSANEYDGIMVKRLEASNTLDEGRTTNQTHIAISGEQMNMFPYLMADGYFKCDYNDRDEQLKKY
FITQIPLYIYKDNVLYLADGEESGISFGDEKSRCVRASIVRSRRKEQADQVQLSLTTIDDPAFVSFRRLLHTGDYLVILK
HKEKLVYDCIGIKANDETRGEFNISALNNKFYKLPTNTKVDIKELIEIKNAEIENKKFTVDELGTILKEMYGNAEDKMQV
ASIHIFGIKYGKNIIENEFKAPDIIKAAGLNESYSTELQKALNIYRCLNKNTYGISISEGDNVLEENKESVKRKTGAENI
LLYGVPGAGKSHEIKTKYCDDEKYMERVVFHPDYTYSDFVGQILPRVEKDRDGNDKLKYVFTPGPYTKLLKKAQNDPGNY
YYLVIEELNRGNAPAIFGEVFQLLDRKDEEDFPLEEVGESEYGISNYEVAKEVYGDEKHQVRIPSNMYVLATMNTADQNV
FTLDTAFQRRWNMRQIENNFDKSEHSKDIIAGTKVSWGAFATVINDMVIDINVDMASSEDKRLGTYFARKKELEAGRFSE
KVLKYLWDDAFKMDKTAIFNENCKSLEDVVITYETATSDKLAAVLRMSVYEKMLSKMQQKNTDNNEN
>Mature_627_residues
MEFTEAIEIIKNIVPESSANEYDGIMVKRLEASNTLDEGRTTNQTHIAISGEQMNMFPYLMADGYFKCDYNDRDEQLKKY
FITQIPLYIYKDNVLYLADGEESGISFGDEKSRCVRASIVRSRRKEQADQVQLSLTTIDDPAFVSFRRLLHTGDYLVILK
HKEKLVYDCIGIKANDETRGEFNISALNNKFYKLPTNTKVDIKELIEIKNAEIENKKFTVDELGTILKEMYGNAEDKMQV
ASIHIFGIKYGKNIIENEFKAPDIIKAAGLNESYSTELQKALNIYRCLNKNTYGISISEGDNVLEENKESVKRKTGAENI
LLYGVPGAGKSHEIKTKYCDDEKYMERVVFHPDYTYSDFVGQILPRVEKDRDGNDKLKYVFTPGPYTKLLKKAQNDPGNY
YYLVIEELNRGNAPAIFGEVFQLLDRKDEEDFPLEEVGESEYGISNYEVAKEVYGDEKHQVRIPSNMYVLATMNTADQNV
FTLDTAFQRRWNMRQIENNFDKSEHSKDIIAGTKVSWGAFATVINDMVIDINVDMASSEDKRLGTYFARKKELEAGRFSE
KVLKYLWDDAFKMDKTAIFNENCKSLEDVVITYETATSDKLAAVLRMSVYEKMLSKMQQKNTDNNEN

Specific function: Recognizes the double-stranded sequence CTCGAG; the cleavage site is unknown [H]

COG id: COG1401

COG function: function code V; GTPase subunit of restriction endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI87082429, Length=199, Percent_Identity=28.643216080402, Blast_Score=81, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011704 [H]

Pfam domain/function: PF07728 AAA_5 [H]

EC number: =3.1.21.4 [H]

Molecular weight: Translated: 72018; Mature: 72018

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEFTEAIEIIKNIVPESSANEYDGIMVKRLEASNTLDEGRTTNQTHIAISGEQMNMFPYL
CCHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCEEEEEECCCCCCCEEE
MADGYFKCDYNDRDEQLKKYFITQIPLYIYKDNVLYLADGEESGISFGDEKSRCVRASIV
CCCCEEEECCCCHHHHHHHHHHHHCCEEEEECCEEEEECCCCCCCCCCCCHHHHHHHHHH
RSRRKEQADQVQLSLTTIDDPAFVSFRRLLHTGDYLVILKHKEKLVYDCIGIKANDETRG
HHHHHHCCCEEEEEEEECCCHHHHHHHHHHHCCCEEEEEECCCCEEEHEECCCCCCCCCC
EFNISALNNKFYKLPTNTKVDIKELIEIKNAEIENKKFTVDELGTILKEMYGNAEDKMQV
EEEEEEECCCEEECCCCCCCCHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHCCCCCCEEE
ASIHIFGIKYGKNIIENEFKAPDIIKAAGLNESYSTELQKALNIYRCLNKNTYGISISEG
EEEEEEEEECCCHHHHCCCCCCCCCHHCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCC
DNVLEENKESVKRKTGAENILLYGVPGAGKSHEIKTKYCDDEKYMERVVFHPDYTYSDFV
CCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHEEECCCCCHHHHH
GQILPRVEKDRDGNDKLKYVFTPGPYTKLLKKAQNDPGNYYYLVIEELNRGNAPAIFGEV
HHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHCCCCCCEEEEEEEECCCCCCCHHHHHH
FQLLDRKDEEDFPLEEVGESEYGISNYEVAKEVYGDEKHQVRIPSNMYVLATMNTADQNV
HHHHCCCCCCCCCHHHCCCCCCCCCHHHHHHHHHCCCCCEEECCCCEEEEEEECCCCCCE
FTLDTAFQRRWNMRQIENNFDKSEHSKDIIAGTKVSWGAFATVINDMVIDINVDMASSED
EEEEHHHHHCCCHHHHHHCCCCCCCCCHHHCCCCCCHHHHHHHHCCEEEEEEEECCCCCC
KRLGTYFARKKELEAGRFSEKVLKYLWDDAFKMDKTAIFNENCKSLEDVVITYETATSDK
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHCCCHHCCCCEEEEEECCCCHH
LAAVLRMSVYEKMLSKMQQKNTDNNEN
HHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MEFTEAIEIIKNIVPESSANEYDGIMVKRLEASNTLDEGRTTNQTHIAISGEQMNMFPYL
CCHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCEEEEEECCCCCCCEEE
MADGYFKCDYNDRDEQLKKYFITQIPLYIYKDNVLYLADGEESGISFGDEKSRCVRASIV
CCCCEEEECCCCHHHHHHHHHHHHCCEEEEECCEEEEECCCCCCCCCCCCHHHHHHHHHH
RSRRKEQADQVQLSLTTIDDPAFVSFRRLLHTGDYLVILKHKEKLVYDCIGIKANDETRG
HHHHHHCCCEEEEEEEECCCHHHHHHHHHHHCCCEEEEEECCCCEEEHEECCCCCCCCCC
EFNISALNNKFYKLPTNTKVDIKELIEIKNAEIENKKFTVDELGTILKEMYGNAEDKMQV
EEEEEEECCCEEECCCCCCCCHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHCCCCCCEEE
ASIHIFGIKYGKNIIENEFKAPDIIKAAGLNESYSTELQKALNIYRCLNKNTYGISISEG
EEEEEEEEECCCHHHHCCCCCCCCCHHCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCC
DNVLEENKESVKRKTGAENILLYGVPGAGKSHEIKTKYCDDEKYMERVVFHPDYTYSDFV
CCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHEEECCCCCHHHHH
GQILPRVEKDRDGNDKLKYVFTPGPYTKLLKKAQNDPGNYYYLVIEELNRGNAPAIFGEV
HHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHCCCCCCEEEEEEEECCCCCCCHHHHHH
FQLLDRKDEEDFPLEEVGESEYGISNYEVAKEVYGDEKHQVRIPSNMYVLATMNTADQNV
HHHHCCCCCCCCCHHHCCCCCCCCCHHHHHHHHHCCCCCEEECCCCEEEEEEECCCCCCE
FTLDTAFQRRWNMRQIENNFDKSEHSKDIIAGTKVSWGAFATVINDMVIDINVDMASSED
EEEEHHHHHCCCHHHHHHCCCCCCCCCHHHCCCCCCHHHHHHHHCCEEEEEEEECCCCCC
KRLGTYFARKKELEAGRFSEKVLKYLWDDAFKMDKTAIFNENCKSLEDVVITYETATSDK
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHCCCHHCCCCEEEEEECCCCHH
LAAVLRMSVYEKMLSKMQQKNTDNNEN
HHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9455482; 9384377 [H]