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The map label for this gene is llaDCHIA [H]

Identifier: 238917690

GI number: 238917690

Start: 1833984

End: 1834817

Strand: Reverse

Name: llaDCHIA [H]

Synonym: EUBELI_01770

Alternate gene names: 238917690

Gene position: 1834817-1833984 (Counterclockwise)

Preceding gene: 238917691

Following gene: 238917689

Centisome position: 85.57

GC content: 37.77

Gene sequence:

>834_bases
ATGAGTAATTCAAGTGTAGCTCCATTTGTGAAATGGGCTGGTGGGAAGCGTCAGTTAATTCCGCAAATAAAGGAGAGAAT
GCCTGAAAAATATAATGATTATTATGAGCCATTTGTGGGTGGCGGTGCAGTTACATTCGAATTGCTACCTGCAAATGCTT
TGATAAACGATATCAACAAAGCGTTGATAAATGCTTACAGGCAGATATGTAATGCACCGGAAGCATTTCTGAAGGCAGTA
AAGAAGCTGGATGAGGAAATGTGGGAGGACGGCAAAGAATATTATTATTCTCTTAGGGAGCATTACAATGATAAGCTGAT
GAAAGCGGAGTTTGATGTGGAGTTGGCTGCATTGTTTGTATTTATCAATAAGCACTGTTTTAACGGCTTGTATCGTGTAA
ATGGCAAGGGACTTTTCAATGTTCCATACAACAATAGTCGTAGGGTTTCAGTTGATGCAGAGGTTATTATGGCTACTTCA
AATTACTTGCAGGGAGTAACCATTATAGATGGTGATTTTGAACTGGCTTGTAAGGATGCAAAGAAGGGCGATTTTGTATT
TATTGACAGCCCGTATGCACCATTGAATCCTACCTCTTTTGAATCATACACAAAAGAGGGTTTTGATATAGAAAGTCATA
AGCGACTAGCAAATCTTTTCGATGAATTAACAGCCAGAGGTTGCTACTGTATGCTCACAAACCATAATACAGACTTGATA
AATGAACTGTATGGTAACAAAGGCTATAAAATAGATGTTGTAAGTGTAAAGCGTATGATTAATTCAGATGCATCTAACAG
AGTTGGCGAAGAAGTGATTATATGCAATTATTAA

Upstream 100 bases:

>100_bases
TCACAAAGAGTGAAAGTTTTGGTTGTTATTTTGTATGTTTGCAAGTATAATAAATGAGTAAAAGATTGTCCTTTTGCGGA
CATAGAGAAGAGGAATCAAA

Downstream 100 bases:

>100_bases
GGAGATAAAGCAATGGGATATTTTGATGAACAACAAAGAATTGTTGATTATGTCACTCACGATGGTATGATAATAGAATT
AACACAAAATAAGGATAATT

Product: DNA adenine methylase

Products: NA

Alternate protein names: M.LlaDCHI A; M.LlaDCHIA; Adenine-specific methyltransferase LlaDCHIA; M.LlaII A [H]

Number of amino acids: Translated: 277; Mature: 276

Protein sequence:

>277_residues
MSNSSVAPFVKWAGGKRQLIPQIKERMPEKYNDYYEPFVGGGAVTFELLPANALINDINKALINAYRQICNAPEAFLKAV
KKLDEEMWEDGKEYYYSLREHYNDKLMKAEFDVELAALFVFINKHCFNGLYRVNGKGLFNVPYNNSRRVSVDAEVIMATS
NYLQGVTIIDGDFELACKDAKKGDFVFIDSPYAPLNPTSFESYTKEGFDIESHKRLANLFDELTARGCYCMLTNHNTDLI
NELYGNKGYKIDVVSVKRMINSDASNRVGEEVIICNY

Sequences:

>Translated_277_residues
MSNSSVAPFVKWAGGKRQLIPQIKERMPEKYNDYYEPFVGGGAVTFELLPANALINDINKALINAYRQICNAPEAFLKAV
KKLDEEMWEDGKEYYYSLREHYNDKLMKAEFDVELAALFVFINKHCFNGLYRVNGKGLFNVPYNNSRRVSVDAEVIMATS
NYLQGVTIIDGDFELACKDAKKGDFVFIDSPYAPLNPTSFESYTKEGFDIESHKRLANLFDELTARGCYCMLTNHNTDLI
NELYGNKGYKIDVVSVKRMINSDASNRVGEEVIICNY
>Mature_276_residues
SNSSVAPFVKWAGGKRQLIPQIKERMPEKYNDYYEPFVGGGAVTFELLPANALINDINKALINAYRQICNAPEAFLKAVK
KLDEEMWEDGKEYYYSLREHYNDKLMKAEFDVELAALFVFINKHCFNGLYRVNGKGLFNVPYNNSRRVSVDAEVIMATSN
YLQGVTIIDGDFELACKDAKKGDFVFIDSPYAPLNPTSFESYTKEGFDIESHKRLANLFDELTARGCYCMLTNHNTDLIN
ELYGNKGYKIDVVSVKRMINSDASNRVGEEVIICNY

Specific function: This methylase recognizes the double-stranded sequence GATC, causes specific methylation on A-2 on both strands, and protects the DNA from cleavage by the LlaDCHI endonuclease [H]

COG id: COG0338

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789789, Length=260, Percent_Identity=30.7692307692308, Blast_Score=119, Evalue=3e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023095
- InterPro:   IPR002052
- InterPro:   IPR012327 [H]

Pfam domain/function: PF02086 MethyltransfD12 [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 31545; Mature: 31414

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNSSVAPFVKWAGGKRQLIPQIKERMPEKYNDYYEPFVGGGAVTFELLPANALINDINK
CCCCCCCCHHEECCCCHHHHHHHHHHCCHHHHHHHCCCCCCCEEEEEEECHHHHHHHHHH
ALINAYRQICNAPEAFLKAVKKLDEEMWEDGKEYYYSLREHYNDKLMKAEFDVELAALFV
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCHHHHHHHCCHHHHHHHH
FINKHCFNGLYRVNGKGLFNVPYNNSRRVSVDAEVIMATSNYLQGVTIIDGDFELACKDA
HHHHHHHCCEEEECCCEEEECCCCCCEEEEECCEEEEEEHHHHCEEEEECCCCEEEECCC
KKGDFVFIDSPYAPLNPTSFESYTKEGFDIESHKRLANLFDELTARGCYCMLTNHNTDLI
CCCCEEEEECCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCHHHH
NELYGNKGYKIDVVSVKRMINSDASNRVGEEVIICNY
HHHHCCCCCEEEHHHHHHHHCCCCCCCCCCEEEEECC
>Mature Secondary Structure 
SNSSVAPFVKWAGGKRQLIPQIKERMPEKYNDYYEPFVGGGAVTFELLPANALINDINK
CCCCCCCHHEECCCCHHHHHHHHHHCCHHHHHHHCCCCCCCEEEEEEECHHHHHHHHHH
ALINAYRQICNAPEAFLKAVKKLDEEMWEDGKEYYYSLREHYNDKLMKAEFDVELAALFV
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCHHHHHHHCCHHHHHHHH
FINKHCFNGLYRVNGKGLFNVPYNNSRRVSVDAEVIMATSNYLQGVTIIDGDFELACKDA
HHHHHHHCCEEEECCCEEEECCCCCCEEEEECCEEEEEEHHHHCEEEEECCCCEEEECCC
KKGDFVFIDSPYAPLNPTSFESYTKEGFDIESHKRLANLFDELTARGCYCMLTNHNTDLI
CCCCEEEEECCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCHHHH
NELYGNKGYKIDVVSVKRMINSDASNRVGEEVIICNY
HHHHCCCCCEEEHHHHHHHHCCCCCCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7793939; 11467810 [H]