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The map label for this gene is 238917344

Identifier: 238917344

GI number: 238917344

Start: 1474073

End: 1474945

Strand: Reverse

Name: 238917344

Synonym: EUBELI_01421

Alternate gene names: NA

Gene position: 1474945-1474073 (Counterclockwise)

Preceding gene: 238917345

Following gene: 238917342

Centisome position: 68.79

GC content: 43.53

Gene sequence:

>873_bases
GTGAGAAATGTAAAGTATGCAGGCATACAGATGCAGTGTTCACGGTCGGTAGAAGAGAACATTGCAAAGGCTGATAAGAT
GGTTCGTGAAGCGGCAGCTAACGGTGCACAGATAATACTGCTTCCTGAGCTTTTTGAAAGACAGTATTTCTGTCAGGAGA
GAAATTATGATTATTACGCATTTGCAACACCTGTTGATGAGAATCCGGCTGTGAAGCATTTTCAGAAGCTGGCAGCCGAA
CTTAAAGTTGTGCTTCCAATAAGCTTTTATGAAAGAGATATAAATGTGTTCTATAACACTGTTGCAGTTATTGACGCAGA
TGGAAGTGTTCTTGGAATATACAGAAAAACACATATTCCTGATGACCATTATTATCAGGAAAAGTTCTATTTCACACCGG
GAGATACAGGTTTTAAGGTGTGGGATACAAGATATGCGAGAATTGGAGTAGGAATATGCTGGGATCAGTGGTTTCCAGAG
ACAGCCAGAGGAATGGCGGTACAGGGAGCAGAAATCCTTTTCTATCCGACAGCGATTGGCTCAGAGCCGATTCTTGAGGT
TGACAGCATGCCGCACTGGAGAAGATGTATGCAGGGACATTCAGCGTGCAATATAGTTCCTGTAGTAGCTGCCAACCGTA
TTGGTGAGGAGAAGGTTACACCATCTGAAGCTAATGGTTATCAGGAATCATCACTTTTATTCTATGGTTCTTCATTTGTA
ACAGATGCAACAGGCGAGATAGTGACACAGGCATCAAGAGACAAGGAAGAAATTGTCTATGGGGAATCCGACCTTGACGC
AGATGCCGACCTTCGCGTGAGCTGGGGCCTGTTCAGGGACAGAAGACCAGAAATCTATAAAGGTATCAGATAA

Upstream 100 bases:

>100_bases
TTTCCAGACAGAAAGATAGTTGGGATATATGCGCGAGATATAATAGTCGGTGGAGGTAATATCCACTGTATTACACAACA
GATTCCGGAGGTAAAATAGT

Downstream 100 bases:

>100_bases
AATAAGCCAGTTAATCTTAATTGCTAGATTAACTGGCTTTAATATTATTAGTATGGTGTTAGTTCTCATTAGCGATAGCA
GCTTCTGCTCTTTCTAAATC

Product: beta-ureidopropionase

Products: NA

Alternate protein names: D-N-alpha-carbamilase [H]

Number of amino acids: Translated: 290; Mature: 290

Protein sequence:

>290_residues
MRNVKYAGIQMQCSRSVEENIAKADKMVREAAANGAQIILLPELFERQYFCQERNYDYYAFATPVDENPAVKHFQKLAAE
LKVVLPISFYERDINVFYNTVAVIDADGSVLGIYRKTHIPDDHYYQEKFYFTPGDTGFKVWDTRYARIGVGICWDQWFPE
TARGMAVQGAEILFYPTAIGSEPILEVDSMPHWRRCMQGHSACNIVPVVAANRIGEEKVTPSEANGYQESSLLFYGSSFV
TDATGEIVTQASRDKEEIVYGESDLDADADLRVSWGLFRDRRPEIYKGIR

Sequences:

>Translated_290_residues
MRNVKYAGIQMQCSRSVEENIAKADKMVREAAANGAQIILLPELFERQYFCQERNYDYYAFATPVDENPAVKHFQKLAAE
LKVVLPISFYERDINVFYNTVAVIDADGSVLGIYRKTHIPDDHYYQEKFYFTPGDTGFKVWDTRYARIGVGICWDQWFPE
TARGMAVQGAEILFYPTAIGSEPILEVDSMPHWRRCMQGHSACNIVPVVAANRIGEEKVTPSEANGYQESSLLFYGSSFV
TDATGEIVTQASRDKEEIVYGESDLDADADLRVSWGLFRDRRPEIYKGIR
>Mature_290_residues
MRNVKYAGIQMQCSRSVEENIAKADKMVREAAANGAQIILLPELFERQYFCQERNYDYYAFATPVDENPAVKHFQKLAAE
LKVVLPISFYERDINVFYNTVAVIDADGSVLGIYRKTHIPDDHYYQEKFYFTPGDTGFKVWDTRYARIGVGICWDQWFPE
TARGMAVQGAEILFYPTAIGSEPILEVDSMPHWRRCMQGHSACNIVPVVAANRIGEEKVTPSEANGYQESSLLFYGSSFV
TDATGEIVTQASRDKEEIVYGESDLDADADLRVSWGLFRDRRPEIYKGIR

Specific function: The enzyme catalyzes the hydrolysis of N-carbamoyl-D- amino acids to the corresponding which are useful intermediates in the preparation of beta-lactam antibiotics. Industrial production of beta-lactam antibiotics is now being developed using this enzyme

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI9910460, Length=293, Percent_Identity=31.3993174061433, Blast_Score=131, Evalue=6e-31,
Organism=Homo sapiens, GI7706509, Length=271, Percent_Identity=28.7822878228782, Blast_Score=104, Evalue=9e-23,
Organism=Homo sapiens, GI5031947, Length=286, Percent_Identity=26.9230769230769, Blast_Score=99, Evalue=3e-21,
Organism=Homo sapiens, GI297632348, Length=286, Percent_Identity=26.9230769230769, Blast_Score=99, Evalue=4e-21,
Organism=Homo sapiens, GI297632350, Length=286, Percent_Identity=26.9230769230769, Blast_Score=99, Evalue=5e-21,
Organism=Homo sapiens, GI297632346, Length=187, Percent_Identity=29.9465240641711, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17533173, Length=302, Percent_Identity=30.1324503311258, Blast_Score=123, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI17556280, Length=289, Percent_Identity=26.2975778546713, Blast_Score=84, Evalue=6e-17,
Organism=Saccharomyces cerevisiae, GI6323383, Length=299, Percent_Identity=27.7591973244147, Blast_Score=103, Evalue=3e-23,
Organism=Drosophila melanogaster, GI21358471, Length=275, Percent_Identity=29.4545454545455, Blast_Score=121, Evalue=5e-28,
Organism=Drosophila melanogaster, GI17933642, Length=282, Percent_Identity=23.758865248227, Blast_Score=69, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003010 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: =3.5.1.77 [H]

Molecular weight: Translated: 33025; Mature: 33025

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNVKYAGIQMQCSRSVEENIAKADKMVREAAANGAQIILLPELFERQYFCQERNYDYYA
CCCCEECCEEEEEHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHCCCCEEE
FATPVDENPAVKHFQKLAAELKVVLPISFYERDINVFYNTVAVIDADGSVLGIYRKTHIP
EECCCCCCHHHHHHHHHHHHEEEEEEEHHHHHHHEEEEEEEEEEECCCCEEEEEEECCCC
DDHYYQEKFYFTPGDTGFKVWDTRYARIGVGICWDQWFPETARGMAVQGAEILFYPTAIG
CCCEECEEEEECCCCCCEEEECCEEEEEEEEEEHHHCCCHHHCCEEECCCEEEEEECCCC
SEPILEVDSMPHWRRCMQGHSACNIVPVVAANRIGEEKVTPSEANGYQESSLLFYGSSFV
CCCCEECCCCCHHHHHHCCCCCCCEEEEEHHCCCCCCCCCCCCCCCCCCCEEEEECCCEE
TDATGEIVTQASRDKEEIVYGESDLDADADLRVSWGLFRDRRPEIYKGIR
CCCCHHHHHHCCCCCHHEEECCCCCCCCCCEEEEECCCCCCCCHHHCCCC
>Mature Secondary Structure
MRNVKYAGIQMQCSRSVEENIAKADKMVREAAANGAQIILLPELFERQYFCQERNYDYYA
CCCCEECCEEEEEHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHCCCCEEE
FATPVDENPAVKHFQKLAAELKVVLPISFYERDINVFYNTVAVIDADGSVLGIYRKTHIP
EECCCCCCHHHHHHHHHHHHEEEEEEEHHHHHHHEEEEEEEEEEECCCCEEEEEEECCCC
DDHYYQEKFYFTPGDTGFKVWDTRYARIGVGICWDQWFPETARGMAVQGAEILFYPTAIG
CCCEECEEEEECCCCCCEEEECCEEEEEEEEEEHHHCCCHHHCCEEECCCEEEEEECCCC
SEPILEVDSMPHWRRCMQGHSACNIVPVVAANRIGEEKVTPSEANGYQESSLLFYGSSFV
CCCCEECCCCCHHHHHHCCCCCCCEEEEEHHCCCCCCCCCCCCCCCCCCCEEEEECCCEE
TDATGEIVTQASRDKEEIVYGESDLDADADLRVSWGLFRDRRPEIYKGIR
CCCCHHHHHHCCCCCHHEEECCCCCCCCCCEEEEECCCCCCCCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9648217; 10903946 [H]