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The map label for this gene is gltB [H]

Identifier: 238917333

GI number: 238917333

Start: 1453050

End: 1454534

Strand: Reverse

Name: gltB [H]

Synonym: EUBELI_01410

Alternate gene names: 238917333

Gene position: 1454534-1453050 (Counterclockwise)

Preceding gene: 238917334

Following gene: 238917332

Centisome position: 67.84

GC content: 41.48

Gene sequence:

>1485_bases
ATGGGAAAGCCAACAGGATTTTTAGACTATGAAAGAGTTGAAGCCACAGCGGTTTCACCTAAGGAAAGAATAAAGAATTT
TAATGAATTTCATACACCTCTGTCAGAAGATGAGCAGAGAAAGCAGAGTTCACGCTGCATGGACTGTGGCGTGCCATTCT
GCCAGTCTGGTATGACAATAAAGGGAATGACTAGCGGATGTCCTCTTAATAATCTTATTCCGGAATGGAATGATCTTCTT
TACACAGGAAACTGGGAACAGGCATATCACAGGTTACATAAGACAAGTAACTTCCCTGAATTTACAAGCCGTGTGTGTCC
TGCACTCTGTGAAAAGGCATGTACATGCGGACTTAATGGCAAGGCAGTATGTACTAAAGAGAATGAAATGGCAATCATTG
AACATGCGTATGCCAAAGGTTATGCGAAAGCAAATCCTCCTAAGGTAAGAACTAAGAAAAGAATTGCTGTAGTGGGTTCA
GGTCCTGCCGGACTTGCTGTAGCAGACCAGTTAAATCAGAGAGGACACAGTGTTACTGTATATGAAAGAGCTGACCGTAT
CGGTGGACTTTTAAGATATGGCATTCCTAATATGAAGCTTGAAAAGCATATAATTGACAGAAAGCTTGATATTATGAAGG
AAGAAGGCATTGAGTTCGTAACTAATGCAAATGTTGGTGAGAACATTAAGGCTAAGAAGCTTATGGCAGATTATGATGCT
GTAGTACTTGCGTGTGGTGCTGCTAATCCAAGAGATATCAATGCTCCTGGAAGAGATGCCAATGGAATATACTTTGCAGT
AGATTTCCTTACAGCAACTACAAAGAGTCTTCTTGATTCAGGACTTAAGGATGGCAGATACATTTCTGCAAAGGATAAAA
ATGTTATCGTTATCGGTGGTGGTGATACAGGTAATGACTGTGTCGGAACATGTATAAGACATGGCTGTAAGTCGGTAACA
CAGCTTGAGATGATGCCAAAAGCACCAGATGAGCGTGCGGAGAACAATCCTTGGCCACAGTGGCCTTTAGTCTGCAAGAC
AGATTACGGTCAGGAAGAAGCAATTGCAGTATTCGGACATGATCCAAGAATATATACAACTACAGTTAAGGAATTCAAGA
AGGATAAGAAGGGTAATCTTACAAGTGTTGTTACAGTAAAGCTTGAGTCTAAGACTGATGAAAAGACAGGAAGAAGAATG
ATGGTCCCTGTGGAGGGAACAGAAGCAGAGCTTCCATGTGAGCTTGTACTGATAGCAGCAGGTTTCCTGGGCTCACAGAA
ATATGTTACAGATGCATTTGGCGTTGAGCTTACAGAAAGAACTAATGTAAAGACAGCAGCAGACGGATTTGAAACAAGTG
TACCGGGAGTATTTACAGCAGGAGATATGCATACAGGACAGTCGCTTGTTGTTAAAGCTATAAGACAGGGAAGAGACTGT
GCAAGAGCAGTTGATTACTACCTTATGGGATATACTAATCTGTAA

Upstream 100 bases:

>100_bases
CAGGCTCAGATAGAAGCATTCTATGCGGCTACCAAGTAGGGTGCGCAAGATGTAGAAAAAGGACAGTGCAAGTTTATAAC
ATACATGAAAGGAGCTAACC

Downstream 100 bases:

>100_bases
TTATGAAAAAATAAGCAGTTGTGCATGTTGTATTGTGCATGACTGCTTATTTCATATTTTCTATTGTTTTAATGTATATA
AAGTTGTATTATTAAAAGCA

Product: glutamate synthase (NADH) small chain

Products: NA

Alternate protein names: NADPH-GOGAT [H]

Number of amino acids: Translated: 494; Mature: 493

Protein sequence:

>494_residues
MGKPTGFLDYERVEATAVSPKERIKNFNEFHTPLSEDEQRKQSSRCMDCGVPFCQSGMTIKGMTSGCPLNNLIPEWNDLL
YTGNWEQAYHRLHKTSNFPEFTSRVCPALCEKACTCGLNGKAVCTKENEMAIIEHAYAKGYAKANPPKVRTKKRIAVVGS
GPAGLAVADQLNQRGHSVTVYERADRIGGLLRYGIPNMKLEKHIIDRKLDIMKEEGIEFVTNANVGENIKAKKLMADYDA
VVLACGAANPRDINAPGRDANGIYFAVDFLTATTKSLLDSGLKDGRYISAKDKNVIVIGGGDTGNDCVGTCIRHGCKSVT
QLEMMPKAPDERAENNPWPQWPLVCKTDYGQEEAIAVFGHDPRIYTTTVKEFKKDKKGNLTSVVTVKLESKTDEKTGRRM
MVPVEGTEAELPCELVLIAAGFLGSQKYVTDAFGVELTERTNVKTAADGFETSVPGVFTAGDMHTGQSLVVKAIRQGRDC
ARAVDYYLMGYTNL

Sequences:

>Translated_494_residues
MGKPTGFLDYERVEATAVSPKERIKNFNEFHTPLSEDEQRKQSSRCMDCGVPFCQSGMTIKGMTSGCPLNNLIPEWNDLL
YTGNWEQAYHRLHKTSNFPEFTSRVCPALCEKACTCGLNGKAVCTKENEMAIIEHAYAKGYAKANPPKVRTKKRIAVVGS
GPAGLAVADQLNQRGHSVTVYERADRIGGLLRYGIPNMKLEKHIIDRKLDIMKEEGIEFVTNANVGENIKAKKLMADYDA
VVLACGAANPRDINAPGRDANGIYFAVDFLTATTKSLLDSGLKDGRYISAKDKNVIVIGGGDTGNDCVGTCIRHGCKSVT
QLEMMPKAPDERAENNPWPQWPLVCKTDYGQEEAIAVFGHDPRIYTTTVKEFKKDKKGNLTSVVTVKLESKTDEKTGRRM
MVPVEGTEAELPCELVLIAAGFLGSQKYVTDAFGVELTERTNVKTAADGFETSVPGVFTAGDMHTGQSLVVKAIRQGRDC
ARAVDYYLMGYTNL
>Mature_493_residues
GKPTGFLDYERVEATAVSPKERIKNFNEFHTPLSEDEQRKQSSRCMDCGVPFCQSGMTIKGMTSGCPLNNLIPEWNDLLY
TGNWEQAYHRLHKTSNFPEFTSRVCPALCEKACTCGLNGKAVCTKENEMAIIEHAYAKGYAKANPPKVRTKKRIAVVGSG
PAGLAVADQLNQRGHSVTVYERADRIGGLLRYGIPNMKLEKHIIDRKLDIMKEEGIEFVTNANVGENIKAKKLMADYDAV
VLACGAANPRDINAPGRDANGIYFAVDFLTATTKSLLDSGLKDGRYISAKDKNVIVIGGGDTGNDCVGTCIRHGCKSVTQ
LEMMPKAPDERAENNPWPQWPLVCKTDYGQEEAIAVFGHDPRIYTTTVKEFKKDKKGNLTSVVTVKLESKTDEKTGRRMM
VPVEGTEAELPCELVLIAAGFLGSQKYVTDAFGVELTERTNVKTAADGFETSVPGVFTAGDMHTGQSLVVKAIRQGRDCA
RAVDYYLMGYTNL

Specific function: NITROGEN METABOLISM, GLUTAMATE BIOSYNTHESIS. THE CATALYZED REACTION BRINGS TOGETHER THE NITROGEN AND CARBON METABOLISM. [C]

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=315, Percent_Identity=23.8095238095238, Blast_Score=82, Evalue=1e-15,
Organism=Escherichia coli, GI1789606, Length=490, Percent_Identity=33.6734693877551, Blast_Score=253, Evalue=2e-68,
Organism=Escherichia coli, GI1788811, Length=484, Percent_Identity=33.0578512396694, Blast_Score=246, Evalue=3e-66,
Organism=Escherichia coli, GI87082180, Length=486, Percent_Identity=32.0987654320988, Blast_Score=210, Evalue=1e-55,
Organism=Escherichia coli, GI1788468, Length=452, Percent_Identity=24.1150442477876, Blast_Score=122, Evalue=7e-29,
Organism=Escherichia coli, GI1789243, Length=441, Percent_Identity=25.8503401360544, Blast_Score=85, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17570289, Length=499, Percent_Identity=42.4849699398798, Blast_Score=434, Evalue=1e-122,
Organism=Caenorhabditis elegans, GI17543860, Length=165, Percent_Identity=32.1212121212121, Blast_Score=80, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI71984108, Length=310, Percent_Identity=23.8709677419355, Blast_Score=79, Evalue=6e-15,
Organism=Saccharomyces cerevisiae, GI6320030, Length=489, Percent_Identity=44.5807770961145, Blast_Score=435, Evalue=1e-123,
Organism=Drosophila melanogaster, GI28574881, Length=491, Percent_Identity=49.6945010183299, Blast_Score=485, Evalue=1e-137,
Organism=Drosophila melanogaster, GI24665539, Length=491, Percent_Identity=49.6945010183299, Blast_Score=485, Evalue=1e-137,
Organism=Drosophila melanogaster, GI24665547, Length=491, Percent_Identity=49.6945010183299, Blast_Score=484, Evalue=1e-137,
Organism=Drosophila melanogaster, GI24665543, Length=491, Percent_Identity=49.6945010183299, Blast_Score=484, Evalue=1e-137,
Organism=Drosophila melanogaster, GI24640763, Length=340, Percent_Identity=25.8823529411765, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI18858217, Length=340, Percent_Identity=25.8823529411765, Blast_Score=88, Evalue=1e-17,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR006005
- InterPro:   IPR009051
- InterPro:   IPR016040
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.4.1.13 [H]

Molecular weight: Translated: 54237; Mature: 54106

Theoretical pI: Translated: 7.73; Mature: 7.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKPTGFLDYERVEATAVSPKERIKNFNEFHTPLSEDEQRKQSSRCMDCGVPFCQSGMTI
CCCCCCCCCHHHHHEECCCHHHHHCCHHHHCCCCCCHHHHHHHHHHHHCCCCHHHCCCEE
KGMTSGCPLNNLIPEWNDLLYTGNWEQAYHRLHKTSNFPEFTSRVCPALCEKACTCGLNG
EECCCCCCHHHCCCCCCCEEEECCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCC
KAVCTKENEMAIIEHAYAKGYAKANPPKVRTKKRIAVVGSGPAGLAVADQLNQRGHSVTV
CEEEECCCCEEEEEHHHHCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHCCCEEEE
YERADRIGGLLRYGIPNMKLEKHIIDRKLDIMKEEGIEFVTNANVGENIKAKKLMADYDA
EEHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHCCCE
VVLACGAANPRDINAPGRDANGIYFAVDFLTATTKSLLDSGLKDGRYISAKDKNVIVIGG
EEEEECCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHCCCCCCEEEEECCCEEEEEEC
GDTGNDCVGTCIRHGCKSVTQLEMMPKAPDERAENNPWPQWPLVCKTDYGQEEAIAVFGH
CCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEECCCCCCCEEEEECC
DPRIYTTTVKEFKKDKKGNLTSVVTVKLESKTDEKTGRRMMVPVEGTEAELPCELVLIAA
CCEEEEHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCEEEEEECCCCCCCCEEEEEEEH
GFLGSQKYVTDAFGVELTERTNVKTAADGFETSVPGVFTAGDMHTGQSLVVKAIRQGRDC
HHCCCCCHHHHHHCEEEECCCCCCCCCCCCCCCCCCEEECCCCCCCHHHHHHHHHCCHHH
ARAVDYYLMGYTNL
HHHHHHHHHCCCCC
>Mature Secondary Structure 
GKPTGFLDYERVEATAVSPKERIKNFNEFHTPLSEDEQRKQSSRCMDCGVPFCQSGMTI
CCCCCCCCHHHHHEECCCHHHHHCCHHHHCCCCCCHHHHHHHHHHHHCCCCHHHCCCEE
KGMTSGCPLNNLIPEWNDLLYTGNWEQAYHRLHKTSNFPEFTSRVCPALCEKACTCGLNG
EECCCCCCHHHCCCCCCCEEEECCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCC
KAVCTKENEMAIIEHAYAKGYAKANPPKVRTKKRIAVVGSGPAGLAVADQLNQRGHSVTV
CEEEECCCCEEEEEHHHHCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHCCCEEEE
YERADRIGGLLRYGIPNMKLEKHIIDRKLDIMKEEGIEFVTNANVGENIKAKKLMADYDA
EEHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHCCCE
VVLACGAANPRDINAPGRDANGIYFAVDFLTATTKSLLDSGLKDGRYISAKDKNVIVIGG
EEEEECCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHCCCCCCEEEEECCCEEEEEEC
GDTGNDCVGTCIRHGCKSVTQLEMMPKAPDERAENNPWPQWPLVCKTDYGQEEAIAVFGH
CCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEECCCCCCCEEEEECC
DPRIYTTTVKEFKKDKKGNLTSVVTVKLESKTDEKTGRRMMVPVEGTEAELPCELVLIAA
CCEEEEHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCEEEEEECCCCCCCCEEEEEEEH
GFLGSQKYVTDAFGVELTERTNVKTAADGFETSVPGVFTAGDMHTGQSLVVKAIRQGRDC
HHCCCCCHHHHHHCEEEECCCCCCCCCCCCCCCCCCEEECCCCCCCHHHHHHHHHCCHHH
ARAVDYYLMGYTNL
HHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377; 11029411 [H]