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The map label for this gene is pheS [H]

Identifier: 238917268

GI number: 238917268

Start: 1376644

End: 1377699

Strand: Reverse

Name: pheS [H]

Synonym: EUBELI_01343

Alternate gene names: 238917268

Gene position: 1377699-1376644 (Counterclockwise)

Preceding gene: 238917269

Following gene: 238917267

Centisome position: 64.25

GC content: 40.06

Gene sequence:

>1056_bases
ATGATAAAAAGCGAAAGAAGTATGGAGGCTATAAGGATGAAAGAAAGACTTGAAGCAATTAGAGAAAGAGCTTTAGCACA
GATTAATGAAACTGATTCGCTTGATAAACTGAATGATATCAGAGTCGCTTTCCTTGGAAAGAAGGGTGAACTTACAGAAG
TACTTAAAGGTATGAAGGAAGTTGCACCAGAAGACAGACCTAAGGTTGGTCAGATTGTTAATGAGACAAGACAGGCAATT
GAAGAAGTATTAGATAAGGCTAAGACAGAATTAGCTGCCAAGAAGAGAGAACTTCAGTTAAAGAGTGAAGTAATAGATGT
TACTCTTCCATCTAAGAGGGATGAGTTAGGACATAAGCATCCTAATACACTTGCATTAGAAGAAATCCAGAAGATATTCA
TCGGAATGGGTTATGAAGTAGTTGATGGACCAGAAGTTGAGTATGATTACTACAACTTTGAGGCACTTAACATACCAGCT
AACCACCCGGCTAAGGATGAACAGGATACATTCTACATCAATGATAAGATAGTACTCCGTACACAGACTTCCCCTGTTCA
GGCAAGAGTTATGGAGAAGGGCAAGCTCCCTATCAGAATGATTTCTCCAGGACGTGTATACAGAGCAGATGAAGTTGATG
CTACACATTCACCATGCTTCCATCAGGTAGAAGGTCTTGTAATTGATAAGGGAATCACATTTGCAGACCTTAAAGGAACT
TTAGCAGAATTCGCTAAGGAGCTTTTCGGACCTGATACTAAAGTTAAATTCAGACCACATCATTTCCCATTCACTGAGCC
TAGTGCCGAGATGGATGTAAGCTGTTTCAAATGTGGTGGTAAAGGATGCCGTTTCTGTAAGGGCGAGGGATGGATTGAAA
TCCTCGGCTGTGGAATGGTTCACCCACATGTATTCGAGCTTTGCGGAATTGATAAGGAAGAATATACAGGATTTGCCTTT
GGTGTAGGACTTGAGAGAGTTGCTCTTCTTAAGTACGAAATCGATGACATGAGACTTCTTTATGAGAACGATATCAGATT
TATAAAGCAGTTCTAA

Upstream 100 bases:

>100_bases
TGGAGCTATGCGCGTAATAGAAATGTACGAGAAGAGACATTCACGGGTTGCAAAATAAAATCGATATGTTACAATAAACA
GTGGCTTTTTACGCATATGA

Downstream 100 bases:

>100_bases
ATCGATTCATATAGTGGTCTAATGACTGGAATAAGATAAAGTATATTTAAGGAGAATGACGATATGAATACATCATTAAA
TTGGATAAAAGCAATGGTTC

Product: phenylalanyl-tRNA synthetase

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase alpha chain; PheRS [H]

Number of amino acids: Translated: 351; Mature: 351

Protein sequence:

>351_residues
MIKSERSMEAIRMKERLEAIRERALAQINETDSLDKLNDIRVAFLGKKGELTEVLKGMKEVAPEDRPKVGQIVNETRQAI
EEVLDKAKTELAAKKRELQLKSEVIDVTLPSKRDELGHKHPNTLALEEIQKIFIGMGYEVVDGPEVEYDYYNFEALNIPA
NHPAKDEQDTFYINDKIVLRTQTSPVQARVMEKGKLPIRMISPGRVYRADEVDATHSPCFHQVEGLVIDKGITFADLKGT
LAEFAKELFGPDTKVKFRPHHFPFTEPSAEMDVSCFKCGGKGCRFCKGEGWIEILGCGMVHPHVFELCGIDKEEYTGFAF
GVGLERVALLKYEIDDMRLLYENDIRFIKQF

Sequences:

>Translated_351_residues
MIKSERSMEAIRMKERLEAIRERALAQINETDSLDKLNDIRVAFLGKKGELTEVLKGMKEVAPEDRPKVGQIVNETRQAI
EEVLDKAKTELAAKKRELQLKSEVIDVTLPSKRDELGHKHPNTLALEEIQKIFIGMGYEVVDGPEVEYDYYNFEALNIPA
NHPAKDEQDTFYINDKIVLRTQTSPVQARVMEKGKLPIRMISPGRVYRADEVDATHSPCFHQVEGLVIDKGITFADLKGT
LAEFAKELFGPDTKVKFRPHHFPFTEPSAEMDVSCFKCGGKGCRFCKGEGWIEILGCGMVHPHVFELCGIDKEEYTGFAF
GVGLERVALLKYEIDDMRLLYENDIRFIKQF
>Mature_351_residues
MIKSERSMEAIRMKERLEAIRERALAQINETDSLDKLNDIRVAFLGKKGELTEVLKGMKEVAPEDRPKVGQIVNETRQAI
EEVLDKAKTELAAKKRELQLKSEVIDVTLPSKRDELGHKHPNTLALEEIQKIFIGMGYEVVDGPEVEYDYYNFEALNIPA
NHPAKDEQDTFYINDKIVLRTQTSPVQARVMEKGKLPIRMISPGRVYRADEVDATHSPCFHQVEGLVIDKGITFADLKGT
LAEFAKELFGPDTKVKFRPHHFPFTEPSAEMDVSCFKCGGKGCRFCKGEGWIEILGCGMVHPHVFELCGIDKEEYTGFAF
GVGLERVALLKYEIDDMRLLYENDIRFIKQF

Specific function: Unknown

COG id: COG0016

COG function: function code J; Phenylalanyl-tRNA synthetase alpha subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI5729820, Length=237, Percent_Identity=32.4894514767932, Blast_Score=129, Evalue=4e-30,
Organism=Homo sapiens, GI4758340, Length=269, Percent_Identity=30.8550185873606, Blast_Score=126, Evalue=2e-29,
Organism=Escherichia coli, GI1788007, Length=328, Percent_Identity=46.9512195121951, Blast_Score=336, Evalue=1e-93,
Organism=Caenorhabditis elegans, GI32566635, Length=249, Percent_Identity=33.7349397590361, Blast_Score=136, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI17508957, Length=303, Percent_Identity=30.03300330033, Blast_Score=132, Evalue=4e-31,
Organism=Caenorhabditis elegans, GI32563657, Length=303, Percent_Identity=30.03300330033, Blast_Score=131, Evalue=5e-31,
Organism=Saccharomyces cerevisiae, GI6321087, Length=373, Percent_Identity=30.2949061662198, Blast_Score=138, Evalue=2e-33,
Organism=Saccharomyces cerevisiae, GI6325304, Length=160, Percent_Identity=33.75, Blast_Score=84, Evalue=5e-17,
Organism=Drosophila melanogaster, GI18858079, Length=268, Percent_Identity=32.4626865671642, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI17137424, Length=248, Percent_Identity=30.6451612903226, Blast_Score=126, Evalue=2e-29,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006195
- InterPro:   IPR004529
- InterPro:   IPR004188
- InterPro:   IPR022911
- InterPro:   IPR002319
- InterPro:   IPR010978 [H]

Pfam domain/function: PF02912 Phe_tRNA-synt_N; PF01409 tRNA-synt_2d [H]

EC number: =6.1.1.20 [H]

Molecular weight: Translated: 39942; Mature: 39942

Theoretical pI: Translated: 5.54; Mature: 5.54

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKSERSMEAIRMKERLEAIRERALAQINETDSLDKLNDIRVAFLGKKGELTEVLKGMKE
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCEEEEEECCCCCHHHHHHHHHH
VAPEDRPKVGQIVNETRQAIEEVLDKAKTELAAKKRELQLKSEVIDVTLPSKRDELGHKH
HCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCHHHHCCCC
PNTLALEEIQKIFIGMGYEVVDGPEVEYDYYNFEALNIPANHPAKDEQDTFYINDKIVLR
CCHHHHHHHHHHHHHCCEEECCCCCCEEEEEEEEEEECCCCCCCCCCCCEEEECCEEEEE
TQTSPVQARVMEKGKLPIRMISPGRVYRADEVDATHSPCFHQVEGLVIDKGITFADLKGT
ECCCCHHHHHHHCCCCCEEEECCCCEEECCCCCCCCCCHHHHHCCEEEECCCEEHHHHHH
LAEFAKELFGPDTKVKFRPHHFPFTEPSAEMDVSCFKCGGKGCRFCKGEGWIEILGCGMV
HHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCHHEECCCCCCCEECCCCEEEEEECCCC
HPHVFELCGIDKEEYTGFAFGVGLERVALLKYEIDDMRLLYENDIRFIKQF
CHHHHHHHCCCHHHCCCEEECCCHHHHHHEEECCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIKSERSMEAIRMKERLEAIRERALAQINETDSLDKLNDIRVAFLGKKGELTEVLKGMKE
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCEEEEEECCCCCHHHHHHHHHH
VAPEDRPKVGQIVNETRQAIEEVLDKAKTELAAKKRELQLKSEVIDVTLPSKRDELGHKH
HCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCHHHHCCCC
PNTLALEEIQKIFIGMGYEVVDGPEVEYDYYNFEALNIPANHPAKDEQDTFYINDKIVLR
CCHHHHHHHHHHHHHCCEEECCCCCCEEEEEEEEEEECCCCCCCCCCCCEEEECCEEEEE
TQTSPVQARVMEKGKLPIRMISPGRVYRADEVDATHSPCFHQVEGLVIDKGITFADLKGT
ECCCCHHHHHHHCCCCCEEEECCCCEEECCCCCCCCCCHHHHHCCEEEECCCEEHHHHHH
LAEFAKELFGPDTKVKFRPHHFPFTEPSAEMDVSCFKCGGKGCRFCKGEGWIEILGCGMV
HHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCHHEECCCCCCCEECCCCEEEEEECCCC
HPHVFELCGIDKEEYTGFAFGVGLERVALLKYEIDDMRLLYENDIRFIKQF
CHHHHHHHCCCHHHCCCEEECCCHHHHHHEEECCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA