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The map label for this gene is glgX [H]

Identifier: 238917238

GI number: 238917238

Start: 1347370

End: 1349217

Strand: Reverse

Name: glgX [H]

Synonym: EUBELI_01311

Alternate gene names: 238917238

Gene position: 1349217-1347370 (Counterclockwise)

Preceding gene: 238917239

Following gene: 238917237

Centisome position: 62.92

GC content: 37.99

Gene sequence:

>1848_bases
ATGCTTATTAGTAAAGGAAATCCCATGCTTATGGGATGCAGCAGATATAAGGACGGATATAATTTTACATATGAGGCAGA
ATGTGACAGTGCAGCTTTGCTGATTTTTGATGCACATATGAAGCCAAAAGAACGTATAGAACTGGATTCATCAATGAAAT
GTGGCAACATATTTTCAGTATATGTATGTGACAGGAAGCTGGACAAAAGTTTCTATTGCTATGAGATAGATGGCTTAATG
TATCTTGACCCATATGCAAAGGCGATAACGGATTGCGGAAGATTTGGACAGACGGACGAGAAAGATGTGTATCTGGCTGC
GATTGATGTGGCAGATTATGACTGGGAAGATGACAGACCGCTTAATTATGATTACAGCGACTGTATATTCTATAAGATGA
ATGTAAGAGGTTTTACTAAAAGCAGAACATCTAAGGTTAAAGATAAAGGAACATTTTCAGGAATAATTAACAAGATTCCA
TATCTTAAGGAACTTGGAATCACAACTATTGAGTTACAGCCCGCATATGAATTTGATGAAATAGGCCGTTTTCCACAGCT
GACTGATACTATAATGTCAAAATACGGAGCCGGAACACATTACAGCGTGGATAAGAATACGCGGAAAATTAATTACTGGG
GATATACGGGAGGATTTTATTTCGCACCTAAGGCATCATATTCTTCAATTGCTTCAAAGCATCCAGGGGTGTTTAGGGAT
TATACAGTTGAATTTAAGAATGTGGTTAAAGAATTGCACAGAAATGGCATTGAAGTCGTTATGGAAATGTTTTTTACTGA
CGAAAGTACAGGCTTTATCCTGCAGTGTGTAAGACACTGGGTTACAGAATATCATATTGATGGTGTACATGTATATTGTG
ATGAGTCTGCACTTAAAGCACTTTCACAGGATGCACTTTTAGCGGATACCAAGATTATAACTGTATACTGGAATGGTAAA
ACCGGAACGAAGAAGCATATGGCTAATTATAATAATGATTTCCAGAATATTGCGCGCCGCCTGTTAAAGGGTGACGAGAA
TATGCTTGGAGAATTTGCAGCTATATCAAGAAAGAATGAAGCAAACAGTGCAAGTATTAATTATATTGCCAACAACAATG
GATTTACTCTTAATGACCTTGTGAGCTTCGACAGAAAGCATAATGAGCTTAATGGTGAGAATAACAGGGATGGCGAGGAT
TTTAATTTCAGCTGGAACTGTGGCGAGGAAGGTTCGACAAGAAAGAGAAAGATTAAAGAACTAAGAATGCGTCAGATTAA
AAATGCACTGGCATTTGTTTTCTTGTCGGCAGGTACTCCGCTTATACTTGCCGGAGACGAGTTTGGCAATTCACAGAACG
GTAACAATAATCCGTATTGTGTTGACAGTGAACTTTCATGGGTTAACTGGAAGGAAACTAAAGAGGGAAAAGAAATTCTT
GAATGGACAAAGGCTCTTATACAATTCAGACAGAATAATAAGATACTTCATATGCCGCAGTCGCTTACTTTAAGTGACAG
GGTATCATGTGGCTACCCTGATATATCATATCATGGAACTAATGCGTGGTATGCACAGATGAATACATATGACAGACATT
TAGGAATTATGTATTCATGTGTATATGGGGATGAAGAAGACCACAGTCTGATATATGCTGCATATAATATGCACTGGGAG
AATCACAGCTTTGCACTTCCTAAGATTAATGGTACATGGAAGGTTGATATGAGTTCTAATGTATCAGGTGCTGTGATTGA
AGATAATAACAGAAGTGTATGTGTTGAGCCACGGAGCGTTGCAATACTCACAGGAACATATGAAATTCCTGCTAAAAAGG
AGAAATAA

Upstream 100 bases:

>100_bases
CCGATAATAGGCTGGATTGTGACAATATTTATAATGAGATTCTATAAGCTGGATAAGAATACTATGAGGGGCTTGTATAA
AAAATAGAGTGGAGATTAAT

Downstream 100 bases:

>100_bases
TGGATATAAAGAAGCTTGCAGATGTCAAAGACAGATTTGCAGATTATGAGAAGATATTTAATTCAGGTGATTATGACAAA
GCGGCAGATATATTATCTGC

Product: glycoside hydrolase family 13 protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 615; Mature: 615

Protein sequence:

>615_residues
MLISKGNPMLMGCSRYKDGYNFTYEAECDSAALLIFDAHMKPKERIELDSSMKCGNIFSVYVCDRKLDKSFYCYEIDGLM
YLDPYAKAITDCGRFGQTDEKDVYLAAIDVADYDWEDDRPLNYDYSDCIFYKMNVRGFTKSRTSKVKDKGTFSGIINKIP
YLKELGITTIELQPAYEFDEIGRFPQLTDTIMSKYGAGTHYSVDKNTRKINYWGYTGGFYFAPKASYSSIASKHPGVFRD
YTVEFKNVVKELHRNGIEVVMEMFFTDESTGFILQCVRHWVTEYHIDGVHVYCDESALKALSQDALLADTKIITVYWNGK
TGTKKHMANYNNDFQNIARRLLKGDENMLGEFAAISRKNEANSASINYIANNNGFTLNDLVSFDRKHNELNGENNRDGED
FNFSWNCGEEGSTRKRKIKELRMRQIKNALAFVFLSAGTPLILAGDEFGNSQNGNNNPYCVDSELSWVNWKETKEGKEIL
EWTKALIQFRQNNKILHMPQSLTLSDRVSCGYPDISYHGTNAWYAQMNTYDRHLGIMYSCVYGDEEDHSLIYAAYNMHWE
NHSFALPKINGTWKVDMSSNVSGAVIEDNNRSVCVEPRSVAILTGTYEIPAKKEK

Sequences:

>Translated_615_residues
MLISKGNPMLMGCSRYKDGYNFTYEAECDSAALLIFDAHMKPKERIELDSSMKCGNIFSVYVCDRKLDKSFYCYEIDGLM
YLDPYAKAITDCGRFGQTDEKDVYLAAIDVADYDWEDDRPLNYDYSDCIFYKMNVRGFTKSRTSKVKDKGTFSGIINKIP
YLKELGITTIELQPAYEFDEIGRFPQLTDTIMSKYGAGTHYSVDKNTRKINYWGYTGGFYFAPKASYSSIASKHPGVFRD
YTVEFKNVVKELHRNGIEVVMEMFFTDESTGFILQCVRHWVTEYHIDGVHVYCDESALKALSQDALLADTKIITVYWNGK
TGTKKHMANYNNDFQNIARRLLKGDENMLGEFAAISRKNEANSASINYIANNNGFTLNDLVSFDRKHNELNGENNRDGED
FNFSWNCGEEGSTRKRKIKELRMRQIKNALAFVFLSAGTPLILAGDEFGNSQNGNNNPYCVDSELSWVNWKETKEGKEIL
EWTKALIQFRQNNKILHMPQSLTLSDRVSCGYPDISYHGTNAWYAQMNTYDRHLGIMYSCVYGDEEDHSLIYAAYNMHWE
NHSFALPKINGTWKVDMSSNVSGAVIEDNNRSVCVEPRSVAILTGTYEIPAKKEK
>Mature_615_residues
MLISKGNPMLMGCSRYKDGYNFTYEAECDSAALLIFDAHMKPKERIELDSSMKCGNIFSVYVCDRKLDKSFYCYEIDGLM
YLDPYAKAITDCGRFGQTDEKDVYLAAIDVADYDWEDDRPLNYDYSDCIFYKMNVRGFTKSRTSKVKDKGTFSGIINKIP
YLKELGITTIELQPAYEFDEIGRFPQLTDTIMSKYGAGTHYSVDKNTRKINYWGYTGGFYFAPKASYSSIASKHPGVFRD
YTVEFKNVVKELHRNGIEVVMEMFFTDESTGFILQCVRHWVTEYHIDGVHVYCDESALKALSQDALLADTKIITVYWNGK
TGTKKHMANYNNDFQNIARRLLKGDENMLGEFAAISRKNEANSASINYIANNNGFTLNDLVSFDRKHNELNGENNRDGED
FNFSWNCGEEGSTRKRKIKELRMRQIKNALAFVFLSAGTPLILAGDEFGNSQNGNNNPYCVDSELSWVNWKETKEGKEIL
EWTKALIQFRQNNKILHMPQSLTLSDRVSCGYPDISYHGTNAWYAQMNTYDRHLGIMYSCVYGDEEDHSLIYAAYNMHWE
NHSFALPKINGTWKVDMSSNVSGAVIEDNNRSVCVEPRSVAILTGTYEIPAKKEK

Specific function: Hydrolyzes The Alpha-1,6-Glucosidic Linkages In Glycogen Which Has First Been Partially Depolymerized By Phosphorylase. Shows Only Very Little Activity With Native Glycogen. [C]

COG id: COG1523

COG function: function code G; Type II secretory pathway, pullulanase PulA and related glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Escherichia coli, GI2367229, Length=596, Percent_Identity=28.3557046979866, Blast_Score=199, Evalue=5e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR011837
- InterPro:   IPR013783
- InterPro:   IPR014756 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02922 CBM_48 [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 70387; Mature: 70387

Theoretical pI: Translated: 6.02; Mature: 6.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLISKGNPMLMGCSRYKDGYNFTYEAECDSAALLIFDAHMKPKERIELDSSMKCGNIFSV
CEECCCCCEEEEHHHCCCCCCEEEECCCCCEEEEEEECCCCCHHHCCCCCCCCCCCEEEE
YVCDRKLDKSFYCYEIDGLMYLDPYAKAITDCGRFGQTDEKDVYLAAIDVADYDWEDDRP
EEECCCCCCCEEEEEECCEEEECHHHHHHHHHHCCCCCCCCCEEEEEEEEECCCCCCCCC
LNYDYSDCIFYKMNVRGFTKSRTSKVKDKGTFSGIINKIPYLKELGITTIELQPAYEFDE
CCCCCCCEEEEEEECCCCCCCHHHHCCCCCHHHHHHHCCCCHHHCCCEEEEECCCCCHHH
IGRFPQLTDTIMSKYGAGTHYSVDKNTRKINYWGYTGGFYFAPKASYSSIASKHPGVFRD
CCCCCHHHHHHHHHCCCCCEEEECCCCEEEEEEEECCCEEECCCCCHHHHHHCCCCCCHH
YTVEFKNVVKELHRNGIEVVMEMFFTDESTGFILQCVRHWVTEYHIDGVHVYCDESALKA
HHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCHHHHHHHHHHHHEEECCEEEEECHHHHHH
LSQDALLADTKIITVYWNGKTGTKKHMANYNNDFQNIARRLLKGDENMLGEFAAISRKNE
HCCCCEEECCEEEEEEECCCCCCHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCC
ANSASINYIANNNGFTLNDLVSFDRKHNELNGENNRDGEDFNFSWNCGEEGSTRKRKIKE
CCCCEEEEEECCCCEEHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCCCHHHHHHHH
LRMRQIKNALAFVFLSAGTPLILAGDEFGNSQNGNNNPYCVDSELSWVNWKETKEGKEIL
HHHHHHHHHHEEEEEECCCEEEEECCCCCCCCCCCCCCEEECCCCCCCCCHHHHHHHHHH
EWTKALIQFRQNNKILHMPQSLTLSDRVSCGYPDISYHGTNAWYAQMNTYDRHLGIMYSC
HHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCCCCEECCCCEEEEECCCCCCCCCEEEEE
VYGDEEDHSLIYAAYNMHWENHSFALPKINGTWKVDMSSNVSGAVIEDNNRSVCVEPRSV
EECCCCCCEEEEEEEEEEECCCCEECCCCCCEEEEEECCCCCEEEEECCCCEEEECCCEE
AILTGTYEIPAKKEK
EEEEEEEECCCCCCC
>Mature Secondary Structure
MLISKGNPMLMGCSRYKDGYNFTYEAECDSAALLIFDAHMKPKERIELDSSMKCGNIFSV
CEECCCCCEEEEHHHCCCCCCEEEECCCCCEEEEEEECCCCCHHHCCCCCCCCCCCEEEE
YVCDRKLDKSFYCYEIDGLMYLDPYAKAITDCGRFGQTDEKDVYLAAIDVADYDWEDDRP
EEECCCCCCCEEEEEECCEEEECHHHHHHHHHHCCCCCCCCCEEEEEEEEECCCCCCCCC
LNYDYSDCIFYKMNVRGFTKSRTSKVKDKGTFSGIINKIPYLKELGITTIELQPAYEFDE
CCCCCCCEEEEEEECCCCCCCHHHHCCCCCHHHHHHHCCCCHHHCCCEEEEECCCCCHHH
IGRFPQLTDTIMSKYGAGTHYSVDKNTRKINYWGYTGGFYFAPKASYSSIASKHPGVFRD
CCCCCHHHHHHHHHCCCCCEEEECCCCEEEEEEEECCCEEECCCCCHHHHHHCCCCCCHH
YTVEFKNVVKELHRNGIEVVMEMFFTDESTGFILQCVRHWVTEYHIDGVHVYCDESALKA
HHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCHHHHHHHHHHHHEEECCEEEEECHHHHHH
LSQDALLADTKIITVYWNGKTGTKKHMANYNNDFQNIARRLLKGDENMLGEFAAISRKNE
HCCCCEEECCEEEEEEECCCCCCHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCC
ANSASINYIANNNGFTLNDLVSFDRKHNELNGENNRDGEDFNFSWNCGEEGSTRKRKIKE
CCCCEEEEEECCCCEEHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCCCHHHHHHHH
LRMRQIKNALAFVFLSAGTPLILAGDEFGNSQNGNNNPYCVDSELSWVNWKETKEGKEIL
HHHHHHHHHHEEEEEECCCEEEEECCCCCCCCCCCCCCEEECCCCCCCCCHHHHHHHHHH
EWTKALIQFRQNNKILHMPQSLTLSDRVSCGYPDISYHGTNAWYAQMNTYDRHLGIMYSC
HHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCCCCEECCCCEEEEECCCCCCCCCEEEEE
VYGDEEDHSLIYAAYNMHWENHSFALPKINGTWKVDMSSNVSGAVIEDNNRSVCVEPRSV
EECCCCCCEEEEEEEEEEECCCCEECCCCCCEEEEEECCCCCEEEEECCCCEEEECCCEE
AILTGTYEIPAKKEK
EEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Glycosylases; Glycosidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]