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The map label for this gene is nadE [H]

Identifier: 238917216

GI number: 238917216

Start: 1326485

End: 1328464

Strand: Reverse

Name: nadE [H]

Synonym: EUBELI_01289

Alternate gene names: 238917216

Gene position: 1328464-1326485 (Counterclockwise)

Preceding gene: 238917217

Following gene: 238917215

Centisome position: 61.96

GC content: 39.65

Gene sequence:

>1980_bases
ATGTTTGACGAAAAGACATATATTGAAAGAGAGGGCAAATTGAAAGACGGATATATAAGAGCAGCGGCCATGACTCCGAA
GATTAAAGTGGCTGACTGTGAGTATAATATAGAAAATATTAAGGCTCTTATGATTGAGGCTTATAAGAAGGATACTGCGA
TAGCTGTTTTTCCGGAAATGTGTATAACAGGTTATACATGTAATGATTTATTTTTGCATGACAGGCTGTTAGATTCAGCA
TTGGAAGGGCTTGTTTCGATAAAACATTTTTCGGAAAATGTAAAAGGAATGATAAGCTTTGTAGGACTTCCATATGAAAT
GAACGGGAAGCTTTACAATGTTGTTGCAGGAGTTATGAACGGACAGATACTTGGTATGGTCCCGAAAATGTATCTTCCTA
ATTATGGAGAATTCTATGAAAGACGACAGTTCACACCGGGATTCAATGAATGTGTATATGTAAATGTTGATGGTGAGGAA
GTTCCCTTTGGAAGTGAATTATTATTTACATTTGATAATAACAGAAAAGTTAAGATTGGCGTTGAGATATGTGAGGATCT
GTGGACACCACAGCCGCCAAGCATAAAGCATGCTATGAATGGTGCAACTATTATTGTAAATGCATCGGCAAGCAATGAAA
CTATTGGCAAAGATACATATAGGAAACAGCTTGTTTCAGGGCAGTCTGCAAGACTTGTGTGCGGATATGTGTACTCAAGT
GCTGGAGGAGGAGAATCTACCCAGGATATTGTGTTCTCAGCACATAACCTTATATGTGAGAATGGTTCTACACTTGCAGA
GGCACATAAATTTGCTGATGAATCGGTATATGCTGATATTGATGTAGAGAGAATATGCTCTGAACGAAGAAGAATGACAA
CCTATGCAGTTGACGAGAACAGCGCATATACAGAAGTTCAGGCTCATGGACTTATCAATAAAGAGTTAAAACTCATAAGA
TATTTTGATAAAGCTCCTTTCGTACCTTCTGATAAGAAGGAGAGAGACTCAAGATGTGAAGAGATTCTTAATATCCAGTC
ATACGGACTTAAGAAGAGACTGGAACATACGAATTGTAAGAATGCAGTAATCGGAATATCAGGAGGACTTGATTCTACAC
TGGCACTTCTTGTAACTGTAAGGGCATTTGACATGTGCGGCTTTGACAGAAGCGGTATACACTGTATAACGATGCCTTGC
TTTGGTACAACAGACAGAACTTATAATAATGCTGTCAAGCTTACTAAGCAGCTTGGCTGTTCGCTCAGGGAAATCAATAT
CATGAAGGCTGTAAGACAGCATTTTGAAGATATCGGACATGATGAGAATAATCATAATGTGACTTATGAGAACGGTCAGG
CAAGAGAGAGAACACAGATACTTATGGATATTGCCAACCAGACTAACGGAATGGTAATAGGAACAGGTGATATGTCGGAG
CTTGCATTAGGCTGGGCAACATATAATGGCGATCATATGTCAATGTATGGAGTAAATGCATCAGTACCTAAGACACTTGT
AAGGCATTTGGTACAGTTCTATGCAGATACATGCGGCAATGAAGAACTGTCATCAGTTCTTAACGATGTGCTTGATACAC
CGGTAAGTCCTGAGCTTCTTCCACCTAAGGAAGATGGTACTATTGCACAGAAGACAGAAGATCTGGTTGGACCTTATGAG
CTGCACGATTTCTTTATGTATCATATGCTAAGATTCGGTGCAGAGCCTGAGAAGATATTCAGAATAGCAAAGTACGCATT
TGCAGGAGAATATGATGATAAGACTATCTATAAGTGGCTAAGAACGTTCACATGGAGATTCTTTGCGCAGCAGTTTAAGC
GCTCGTGTCTGCCTGACGGACCTAAGGTCGGTTCAGTTGCAGTATCGCCAAGAGGTGATTTAAGAATGCCGAGTGATGCA
TGCTCGCATGAGTGGATGAAACAGTTAGACAGAATTAAAGAAATTAACGGATATGAATAG

Upstream 100 bases:

>100_bases
AGCAGGAAAGCGGCGTGACACTTGTTTATGGACAATATAATCAAAAGTGACTTTTTTGTGACCTTTATTGTAAATATATA
CCCAGTCTGATATAATTTTA

Downstream 100 bases:

>100_bases
GGAGGACATTATGGCTGATAATAATGCGGCATCAGATAAGGAGAAGGTTGTTACTAAATATGACCGTAAGATGCAGAAAC
GAAAAGAAGAAGAGAGAAAA

Product: NAD+ synthase

Products: NA

Alternate protein names: NAD(+) synthase [glutamine-hydrolyzing] [H]

Number of amino acids: Translated: 659; Mature: 659

Protein sequence:

>659_residues
MFDEKTYIEREGKLKDGYIRAAAMTPKIKVADCEYNIENIKALMIEAYKKDTAIAVFPEMCITGYTCNDLFLHDRLLDSA
LEGLVSIKHFSENVKGMISFVGLPYEMNGKLYNVVAGVMNGQILGMVPKMYLPNYGEFYERRQFTPGFNECVYVNVDGEE
VPFGSELLFTFDNNRKVKIGVEICEDLWTPQPPSIKHAMNGATIIVNASASNETIGKDTYRKQLVSGQSARLVCGYVYSS
AGGGESTQDIVFSAHNLICENGSTLAEAHKFADESVYADIDVERICSERRRMTTYAVDENSAYTEVQAHGLINKELKLIR
YFDKAPFVPSDKKERDSRCEEILNIQSYGLKKRLEHTNCKNAVIGISGGLDSTLALLVTVRAFDMCGFDRSGIHCITMPC
FGTTDRTYNNAVKLTKQLGCSLREINIMKAVRQHFEDIGHDENNHNVTYENGQARERTQILMDIANQTNGMVIGTGDMSE
LALGWATYNGDHMSMYGVNASVPKTLVRHLVQFYADTCGNEELSSVLNDVLDTPVSPELLPPKEDGTIAQKTEDLVGPYE
LHDFFMYHMLRFGAEPEKIFRIAKYAFAGEYDDKTIYKWLRTFTWRFFAQQFKRSCLPDGPKVGSVAVSPRGDLRMPSDA
CSHEWMKQLDRIKEINGYE

Sequences:

>Translated_659_residues
MFDEKTYIEREGKLKDGYIRAAAMTPKIKVADCEYNIENIKALMIEAYKKDTAIAVFPEMCITGYTCNDLFLHDRLLDSA
LEGLVSIKHFSENVKGMISFVGLPYEMNGKLYNVVAGVMNGQILGMVPKMYLPNYGEFYERRQFTPGFNECVYVNVDGEE
VPFGSELLFTFDNNRKVKIGVEICEDLWTPQPPSIKHAMNGATIIVNASASNETIGKDTYRKQLVSGQSARLVCGYVYSS
AGGGESTQDIVFSAHNLICENGSTLAEAHKFADESVYADIDVERICSERRRMTTYAVDENSAYTEVQAHGLINKELKLIR
YFDKAPFVPSDKKERDSRCEEILNIQSYGLKKRLEHTNCKNAVIGISGGLDSTLALLVTVRAFDMCGFDRSGIHCITMPC
FGTTDRTYNNAVKLTKQLGCSLREINIMKAVRQHFEDIGHDENNHNVTYENGQARERTQILMDIANQTNGMVIGTGDMSE
LALGWATYNGDHMSMYGVNASVPKTLVRHLVQFYADTCGNEELSSVLNDVLDTPVSPELLPPKEDGTIAQKTEDLVGPYE
LHDFFMYHMLRFGAEPEKIFRIAKYAFAGEYDDKTIYKWLRTFTWRFFAQQFKRSCLPDGPKVGSVAVSPRGDLRMPSDA
CSHEWMKQLDRIKEINGYE
>Mature_659_residues
MFDEKTYIEREGKLKDGYIRAAAMTPKIKVADCEYNIENIKALMIEAYKKDTAIAVFPEMCITGYTCNDLFLHDRLLDSA
LEGLVSIKHFSENVKGMISFVGLPYEMNGKLYNVVAGVMNGQILGMVPKMYLPNYGEFYERRQFTPGFNECVYVNVDGEE
VPFGSELLFTFDNNRKVKIGVEICEDLWTPQPPSIKHAMNGATIIVNASASNETIGKDTYRKQLVSGQSARLVCGYVYSS
AGGGESTQDIVFSAHNLICENGSTLAEAHKFADESVYADIDVERICSERRRMTTYAVDENSAYTEVQAHGLINKELKLIR
YFDKAPFVPSDKKERDSRCEEILNIQSYGLKKRLEHTNCKNAVIGISGGLDSTLALLVTVRAFDMCGFDRSGIHCITMPC
FGTTDRTYNNAVKLTKQLGCSLREINIMKAVRQHFEDIGHDENNHNVTYENGQARERTQILMDIANQTNGMVIGTGDMSE
LALGWATYNGDHMSMYGVNASVPKTLVRHLVQFYADTCGNEELSSVLNDVLDTPVSPELLPPKEDGTIAQKTEDLVGPYE
LHDFFMYHMLRFGAEPEKIFRIAKYAFAGEYDDKTIYKWLRTFTWRFFAQQFKRSCLPDGPKVGSVAVSPRGDLRMPSDA
CSHEWMKQLDRIKEINGYE

Specific function: Can use both glutamine or ammonia as a nitrogen source [H]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI41393551, Length=666, Percent_Identity=24.9249249249249, Blast_Score=126, Evalue=5e-29,
Organism=Caenorhabditis elegans, GI17538756, Length=667, Percent_Identity=24.7376311844078, Blast_Score=121, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6321865, Length=652, Percent_Identity=24.6932515337423, Blast_Score=115, Evalue=3e-26,
Organism=Drosophila melanogaster, GI24641841, Length=630, Percent_Identity=23.4920634920635, Blast_Score=107, Evalue=4e-23,
Organism=Drosophila melanogaster, GI24641843, Length=630, Percent_Identity=23.4920634920635, Blast_Score=107, Evalue=4e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014445
- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR003010
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00795 CN_hydrolase; PF02540 NAD_synthase [H]

EC number: =6.3.5.1 [H]

Molecular weight: Translated: 74253; Mature: 74253

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFDEKTYIEREGKLKDGYIRAAAMTPKIKVADCEYNIENIKALMIEAYKKDTAIAVFPEM
CCCCCCHHCCCCCCCCCEEEEEECCCCEEEEECCCCHHHHHHHHHHHHCCCCEEEEEHHH
CITGYTCNDLFLHDRLLDSALEGLVSIKHFSENVKGMISFVGLPYEMNGKLYNVVAGVMN
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCHHHHHHHHHC
GQILGMVPKMYLPNYGEFYERRQFTPGFNECVYVNVDGEEVPFGSELLFTFDNNRKVKIG
CEEEEECCHHHCCCHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCEEEEEECCCCEEEEH
VEICEDLWTPQPPSIKHAMNGATIIVNASASNETIGKDTYRKQLVSGQSARLVCGYVYSS
HHHHHHCCCCCCCCHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHCCCCCEEEEEEEEEC
AGGGESTQDIVFSAHNLICENGSTLAEAHKFADESVYADIDVERICSERRRMTTYAVDEN
CCCCCCCHHHHHHHHHHEECCCCHHHHHHHHCCCCEEECCCHHHHHHHHHHCEEEEECCC
SAYTEVQAHGLINKELKLIRYFDKAPFVPSDKKERDSRCEEILNIQSYGLKKRLEHTNCK
CCCHHHHHCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
NAVIGISGGLDSTLALLVTVRAFDMCGFDRSGIHCITMPCFGTTDRTYNNAVKLTKQLGC
CEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHCC
SLREINIMKAVRQHFEDIGHDENNHNVTYENGQARERTQILMDIANQTNGMVIGTGDMSE
CHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCHHHHHHHHHHHHCCCCCEEEECCCHHH
LALGWATYNGDHMSMYGVNASVPKTLVRHLVQFYADTCGNEELSSVLNDVLDTPVSPELL
HEEEEEEECCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCC
PPKEDGTIAQKTEDLVGPYELHDFFMYHMLRFGAEPEKIFRIAKYAFAGEYDDKTIYKWL
CCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHH
RTFTWRFFAQQFKRSCLPDGPKVGSVAVSPRGDLRMPSDACSHEWMKQLDRIKEINGYE
HHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MFDEKTYIEREGKLKDGYIRAAAMTPKIKVADCEYNIENIKALMIEAYKKDTAIAVFPEM
CCCCCCHHCCCCCCCCCEEEEEECCCCEEEEECCCCHHHHHHHHHHHHCCCCEEEEEHHH
CITGYTCNDLFLHDRLLDSALEGLVSIKHFSENVKGMISFVGLPYEMNGKLYNVVAGVMN
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCHHHHHHHHHC
GQILGMVPKMYLPNYGEFYERRQFTPGFNECVYVNVDGEEVPFGSELLFTFDNNRKVKIG
CEEEEECCHHHCCCHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCEEEEEECCCCEEEEH
VEICEDLWTPQPPSIKHAMNGATIIVNASASNETIGKDTYRKQLVSGQSARLVCGYVYSS
HHHHHHCCCCCCCCHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHCCCCCEEEEEEEEEC
AGGGESTQDIVFSAHNLICENGSTLAEAHKFADESVYADIDVERICSERRRMTTYAVDEN
CCCCCCCHHHHHHHHHHEECCCCHHHHHHHHCCCCEEECCCHHHHHHHHHHCEEEEECCC
SAYTEVQAHGLINKELKLIRYFDKAPFVPSDKKERDSRCEEILNIQSYGLKKRLEHTNCK
CCCHHHHHCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
NAVIGISGGLDSTLALLVTVRAFDMCGFDRSGIHCITMPCFGTTDRTYNNAVKLTKQLGC
CEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHCC
SLREINIMKAVRQHFEDIGHDENNHNVTYENGQARERTQILMDIANQTNGMVIGTGDMSE
CHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCHHHHHHHHHHHHCCCCCEEEECCCHHH
LALGWATYNGDHMSMYGVNASVPKTLVRHLVQFYADTCGNEELSSVLNDVLDTPVSPELL
HEEEEEEECCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCC
PPKEDGTIAQKTEDLVGPYELHDFFMYHMLRFGAEPEKIFRIAKYAFAGEYDDKTIYKWL
CCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHH
RTFTWRFFAQQFKRSCLPDGPKVGSVAVSPRGDLRMPSDACSHEWMKQLDRIKEINGYE
HHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]