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The map label for this gene is murI

Identifier: 238917207

GI number: 238917207

Start: 1318276

End: 1319073

Strand: Reverse

Name: murI

Synonym: EUBELI_01280

Alternate gene names: 238917207

Gene position: 1319073-1318276 (Counterclockwise)

Preceding gene: 238917208

Following gene: 238917206

Centisome position: 61.52

GC content: 36.22

Gene sequence:

>798_bases
ATGAAGATAGGAATATTTGACTCAGGGATAGGTGGTCTGTCGGTGCTTCATCAGGCGATGATTACCATGCCAGAGGCTGA
TTACATATTTTATGCTGATGTGGATAATGTTCCATATGGAGAAAAAACAAGGGAGGAAGTAAGAAAGCTTGTTGATCATG
CGGTTGGATTTCTTGTTGATAAGGGCTGTCAGGCAATTGTGCTTGCATGTAATACAGCAACAAGTGCAGCAATATCATAT
CTAAGAGAAAAGTATAAGCTGCCAATAATTGGAATAGAGCCGGCAGTCAAGCCTGCAGTTGAACATACTTCTGAAACCGG
AAGAAGGGTTATGGTAGTATCTACACCTGTTACAGCAAAAGGGGAAAAGTTAAAAAGACTTATAGATAAATATGATGATA
AGCATGTTGTTGATGTTGTGGCACTTCCAAAACTTGTAAGGTTCGCACAGGATGATGATTTTGATTCTTCAGATGTGACT
GATTATTTAAAATGTGAATTTGCTCCATACAATCTTAATGATTATTCTGAACTTGTGCTTGGGTGTACACATTTTAACTA
TTTCAAGGATTCATTTGCAAAGCTGTTTCCTGATGATTTAGAGATGGTTGACGGAAATACGGGTGTATCTAATAATCTTA
AGAATACTGTTATGAAAAAAGGGATATTTACTGAGAAAGATAAAGGGAAAAAAGGCAGTGTAGAATATTATTATTCAGAT
AGAAAAATGGAGAGTGAAGCTGAGATGAAACATATAAAAAAGCTTCATGAAAGACTTGAGAGAATGAGACAGATATAG

Upstream 100 bases:

>100_bases
TGCAGGTGAGATAAGACCGAGCAGGGTTAAGATATTAAAGGATAAAATGATTGTTTATTGCAAGGTATATAATGATTAGA
ATATAGAGGATTGTAGCAAT

Downstream 100 bases:

>100_bases
AATTGCCAGAGGAATGTAATTATGGGAAAATCAGACGATAAGACTAATGTATGCAGAGTGCTTGACCAGAAAAAAGTACC
TTATAAAACACATGTGTGCC

Product: glutamate racemase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 265; Mature: 265

Protein sequence:

>265_residues
MKIGIFDSGIGGLSVLHQAMITMPEADYIFYADVDNVPYGEKTREEVRKLVDHAVGFLVDKGCQAIVLACNTATSAAISY
LREKYKLPIIGIEPAVKPAVEHTSETGRRVMVVSTPVTAKGEKLKRLIDKYDDKHVVDVVALPKLVRFAQDDDFDSSDVT
DYLKCEFAPYNLNDYSELVLGCTHFNYFKDSFAKLFPDDLEMVDGNTGVSNNLKNTVMKKGIFTEKDKGKKGSVEYYYSD
RKMESEAEMKHIKKLHERLERMRQI

Sequences:

>Translated_265_residues
MKIGIFDSGIGGLSVLHQAMITMPEADYIFYADVDNVPYGEKTREEVRKLVDHAVGFLVDKGCQAIVLACNTATSAAISY
LREKYKLPIIGIEPAVKPAVEHTSETGRRVMVVSTPVTAKGEKLKRLIDKYDDKHVVDVVALPKLVRFAQDDDFDSSDVT
DYLKCEFAPYNLNDYSELVLGCTHFNYFKDSFAKLFPDDLEMVDGNTGVSNNLKNTVMKKGIFTEKDKGKKGSVEYYYSD
RKMESEAEMKHIKKLHERLERMRQI
>Mature_265_residues
MKIGIFDSGIGGLSVLHQAMITMPEADYIFYADVDNVPYGEKTREEVRKLVDHAVGFLVDKGCQAIVLACNTATSAAISY
LREKYKLPIIGIEPAVKPAVEHTSETGRRVMVVSTPVTAKGEKLKRLIDKYDDKHVVDVVALPKLVRFAQDDDFDSSDVT
DYLKCEFAPYNLNDYSELVLGCTHFNYFKDSFAKLFPDDLEMVDGNTGVSNNLKNTVMKKGIFTEKDKGKKGSVEYYYSD
RKMESEAEMKHIKKLHERLERMRQI

Specific function: Provides the (R)-glutamate required for cell wall biosynthesis

COG id: COG0796

COG function: function code M; Glutamate racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aspartate/glutamate racemases family

Homologues:

Organism=Escherichia coli, GI87082355, Length=211, Percent_Identity=28.9099526066351, Blast_Score=92, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURI_EUBE2 (C4Z114)

Other databases:

- EMBL:   CP001104
- RefSeq:   YP_002930724.1
- GeneID:   7956626
- GenomeReviews:   CP001104_GR
- KEGG:   eel:EUBELI_01280
- OMA:   NFATECK
- HAMAP:   MF_00258
- InterPro:   IPR015942
- InterPro:   IPR001920
- InterPro:   IPR018187
- InterPro:   IPR004391
- Gene3D:   G3DSA:3.40.50.1860
- TIGRFAMs:   TIGR00067

Pfam domain/function: PF01177 Asp_Glu_race; SSF53681 Asp/Glu_race

EC number: =5.1.1.3

Molecular weight: Translated: 29985; Mature: 29985

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: PS00923 ASP_GLU_RACEMASE_1; PS00924 ASP_GLU_RACEMASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIGIFDSGIGGLSVLHQAMITMPEADYIFYADVDNVPYGEKTREEVRKLVDHAVGFLVD
CEEECCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHH
KGCQAIVLACNTATSAAISYLREKYKLPIIGIEPAVKPAVEHTSETGRRVMVVSTPVTAK
CCCEEEEEEECCHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHCCCCEEEEEECCCCCC
GEKLKRLIDKYDDKHVVDVVALPKLVRFAQDDDFDSSDVTDYLKCEFAPYNLNDYSELVL
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHEEECCCCCCCCHHHHHH
GCTHFNYFKDSFAKLFPDDLEMVDGNTGVSNNLKNTVMKKGIFTEKDKGKKGSVEYYYSD
HHHHHHHHHHHHHHHCCCHHHEECCCCCCCCHHHHHHHHHCCCCCCCCCCCCCEEEEECC
RKMESEAEMKHIKKLHERLERMRQI
CCCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKIGIFDSGIGGLSVLHQAMITMPEADYIFYADVDNVPYGEKTREEVRKLVDHAVGFLVD
CEEECCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHH
KGCQAIVLACNTATSAAISYLREKYKLPIIGIEPAVKPAVEHTSETGRRVMVVSTPVTAK
CCCEEEEEEECCHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHCCCCEEEEEECCCCCC
GEKLKRLIDKYDDKHVVDVVALPKLVRFAQDDDFDSSDVTDYLKCEFAPYNLNDYSELVL
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHEEECCCCCCCCHHHHHH
GCTHFNYFKDSFAKLFPDDLEMVDGNTGVSNNLKNTVMKKGIFTEKDKGKKGSVEYYYSD
HHHHHHHHHHHHHHHCCCHHHEECCCCCCCCHHHHHHHHHCCCCCCCCCCCCCEEEEECC
RKMESEAEMKHIKKLHERLERMRQI
CCCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA