Click here to switch to the map view.

The map label for this gene is rlmH

Identifier: 238917149

GI number: 238917149

Start: 1238727

End: 1239206

Strand: Reverse

Name: rlmH

Synonym: EUBELI_01221

Alternate gene names: 238917149

Gene position: 1239206-1238727 (Counterclockwise)

Preceding gene: 238917150

Following gene: 238917148

Centisome position: 57.79

GC content: 38.12

Gene sequence:

>480_bases
ATGCGTATTACGGTTGTATGTGTAGGTAAGATTAAAGAAAAATTTTATACCCAGGCGGTAGATGAGTATTCCAAGAGACT
TTCAAGATATTGTAAGCTCGATATTGTAGAGCTGCCTGATGAGAAAACACCGGATAATGCAAGTGATGTTGTCAACGAGC
AGATTAAGAACAAGGAAGGGGAGAGAATCCTTTCTGCCATTAAAGATGATGCTTATGTATGCGCACTTGCAATAGAAGGA
AAAATGCTTGATTCTGTTGAACTATCAGAAAAGATTGACAGACTTGGAATAGACGGAACAAGCAACATAACATTTGTAAT
AGGAGGCTCTTTGGGACTGGCAGATGCAGTCCTAAAAAGGGCAGATTATAAGCTGAGTTTTTCAAAAATGACATTTCCAC
ATCAGCTTATGAGAGTTATTCTTTTGGAACAGATATACAGGGCTTACAGGATTATGAAGAATGAGCCATATCACAAGTAG

Upstream 100 bases:

>100_bases
GAATGGATGTATCTGAATTAAGTAATGATGAGCTTCTTGAGCAGTTAGAAGTATTCAAACTTGATAATGGCGGATATTTC
TTTGTGGAAGGATAATACAT

Downstream 100 bases:

>100_bases
TTAAAGAGGATGGATTATGAAAAGAAGAAAGAAAACAATTGTAATTATAGCAGTATTTCTTGTAGTTGCGCTTCTTATAA
CAGGATATATCAGGTTTTTT

Product: rRNA large subunit methyltransferase

Products: NA

Alternate protein names: 23S rRNA m3Psi1915 methyltransferase; rRNA (pseudouridine-N3-)-methyltransferase rlmH

Number of amino acids: Translated: 159; Mature: 159

Protein sequence:

>159_residues
MRITVVCVGKIKEKFYTQAVDEYSKRLSRYCKLDIVELPDEKTPDNASDVVNEQIKNKEGERILSAIKDDAYVCALAIEG
KMLDSVELSEKIDRLGIDGTSNITFVIGGSLGLADAVLKRADYKLSFSKMTFPHQLMRVILLEQIYRAYRIMKNEPYHK

Sequences:

>Translated_159_residues
MRITVVCVGKIKEKFYTQAVDEYSKRLSRYCKLDIVELPDEKTPDNASDVVNEQIKNKEGERILSAIKDDAYVCALAIEG
KMLDSVELSEKIDRLGIDGTSNITFVIGGSLGLADAVLKRADYKLSFSKMTFPHQLMRVILLEQIYRAYRIMKNEPYHK
>Mature_159_residues
MRITVVCVGKIKEKFYTQAVDEYSKRLSRYCKLDIVELPDEKTPDNASDVVNEQIKNKEGERILSAIKDDAYVCALAIEG
KMLDSVELSEKIDRLGIDGTSNITFVIGGSLGLADAVLKRADYKLSFSKMTFPHQLMRVILLEQIYRAYRIMKNEPYHK

Specific function: Specifically methylates the pseudouridine at position 1915 (m3Psi1915) in 23S rRNA

COG id: COG1576

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase rlmH family

Homologues:

Organism=Escherichia coli, GI1786855, Length=159, Percent_Identity=33.9622641509434, Blast_Score=100, Evalue=7e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMH_EUBE2 (C4Z0V6)

Other databases:

- EMBL:   CP001104
- RefSeq:   YP_002930666.1
- ProteinModelPortal:   C4Z0V6
- GeneID:   7957763
- GenomeReviews:   CP001104_GR
- KEGG:   eel:EUBELI_01221
- OMA:   TANHPYH
- ProtClustDB:   PRK00103
- GO:   GO:0005737
- HAMAP:   MF_00658
- InterPro:   IPR016051
- InterPro:   IPR003742
- PIRSF:   PIRSF004505
- TIGRFAMs:   TIGR00246

Pfam domain/function: PF02590 SPOUT_MTase

EC number: NA

Molecular weight: Translated: 18126; Mature: 18126

Theoretical pI: Translated: 8.03; Mature: 8.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRITVVCVGKIKEKFYTQAVDEYSKRLSRYCKLDIVELPDEKTPDNASDVVNEQIKNKEG
CEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCHHHHHHHHHHCCHH
ERILSAIKDDAYVCALAIEGKMLDSVELSEKIDRLGIDGTSNITFVIGGSLGLADAVLKR
HHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHH
ADYKLSFSKMTFPHQLMRVILLEQIYRAYRIMKNEPYHK
HCCCEEHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MRITVVCVGKIKEKFYTQAVDEYSKRLSRYCKLDIVELPDEKTPDNASDVVNEQIKNKEG
CEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCHHHHHHHHHHCCHH
ERILSAIKDDAYVCALAIEGKMLDSVELSEKIDRLGIDGTSNITFVIGGSLGLADAVLKR
HHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHH
ADYKLSFSKMTFPHQLMRVILLEQIYRAYRIMKNEPYHK
HCCCEEHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA