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The map label for this gene is rluF [H]

Identifier: 238917131

GI number: 238917131

Start: 1215022

End: 1215756

Strand: Reverse

Name: rluF [H]

Synonym: EUBELI_01202

Alternate gene names: 238917131

Gene position: 1215756-1215022 (Counterclockwise)

Preceding gene: 238917132

Following gene: 238917130

Centisome position: 56.7

GC content: 37.69

Gene sequence:

>735_bases
ATGATGATAAGAATTGGAGGAAACATGTCAGAGCGTATTAATAAATATCTCAGTGCCCATGGAATCTGTTCAAGACGTGA
AGCTGACAGAATGATACAGGAAGGACGTATCACAATTGATGGCAGGATTGCAGCTATGGGTGAGATGGTAGACGACAATA
CAATTATATGTGTTGATGGCAGGAAAGTTGACAACAGCACACCACCACAGGTAGTCATAGCTTTTAATAAGCCTGTAGGA
ATTGTATGTACAACAACTGATAAACAGGGAAAGAATAACATAGTGGATTTTATCGGATATAAGGAACGCATATACCCTGT
GGGCAGATTAGATAAAGAGTCTGATGGTCTTATTCTGCTTACTAATGATGGTGATATGATGGACAGGCTGCTTCGTTCCG
TTAATGGTCATGAGAAGGAATATATCGTTTCAGTTAATAAACCAATAAACAAAGACTTTATAAAGAGAATGTCAGGGGGG
ATATATCTTAAGGAACTCGACAGAACTACAAGAAAATGTGTTGTGGAAAAGATATCAGACAGGACATTCAGAATTATTCT
TACACAGGGGCTTAACAGACAGATAAGAAGAATGTGTGCGGAATGTGGATATAGTGTAACTAAACTGACAAGAATAAGGA
TTATGAATGTGGAATTAGGGGATTTAAAGACTGGTGAGTACAGGGAACTGGACGGTAAAGAGCTTAATACACTTTATGAG
AAGATATATAAGTAA

Upstream 100 bases:

>100_bases
ATATACTATACATTATTAAATTAAAACCCGCAACAATTGAATTAATTTTTTTCTTTTGTTAGAATTAATACATCTGTATT
TTGATTTGAACTTATCAACA

Downstream 100 bases:

>100_bases
GCAGTAAAAGCTTATATAAGCAGATTTTAATAGTCGATATATAAGCGGCATATAAGGAGCGGATTATGGATTTATTACAG
GAATATTCAGAACTTAAGAA

Product: ribosomal large subunit pseudouridine synthase F

Products: NA

Alternate protein names: rRNA pseudouridylate synthase F; rRNA-uridine isomerase F [H]

Number of amino acids: Translated: 244; Mature: 244

Protein sequence:

>244_residues
MMIRIGGNMSERINKYLSAHGICSRREADRMIQEGRITIDGRIAAMGEMVDDNTIICVDGRKVDNSTPPQVVIAFNKPVG
IVCTTTDKQGKNNIVDFIGYKERIYPVGRLDKESDGLILLTNDGDMMDRLLRSVNGHEKEYIVSVNKPINKDFIKRMSGG
IYLKELDRTTRKCVVEKISDRTFRIILTQGLNRQIRRMCAECGYSVTKLTRIRIMNVELGDLKTGEYRELDGKELNTLYE
KIYK

Sequences:

>Translated_244_residues
MMIRIGGNMSERINKYLSAHGICSRREADRMIQEGRITIDGRIAAMGEMVDDNTIICVDGRKVDNSTPPQVVIAFNKPVG
IVCTTTDKQGKNNIVDFIGYKERIYPVGRLDKESDGLILLTNDGDMMDRLLRSVNGHEKEYIVSVNKPINKDFIKRMSGG
IYLKELDRTTRKCVVEKISDRTFRIILTQGLNRQIRRMCAECGYSVTKLTRIRIMNVELGDLKTGEYRELDGKELNTLYE
KIYK
>Mature_244_residues
MMIRIGGNMSERINKYLSAHGICSRREADRMIQEGRITIDGRIAAMGEMVDDNTIICVDGRKVDNSTPPQVVIAFNKPVG
IVCTTTDKQGKNNIVDFIGYKERIYPVGRLDKESDGLILLTNDGDMMDRLLRSVNGHEKEYIVSVNKPINKDFIKRMSGG
IYLKELDRTTRKCVVEKISDRTFRIILTQGLNRQIRRMCAECGYSVTKLTRIRIMNVELGDLKTGEYRELDGKELNTLYE
KIYK

Specific function: Responsible for synthesis of pseudouridine from uracil- 2604 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790453, Length=234, Percent_Identity=50, Blast_Score=218, Evalue=3e-58,
Organism=Escherichia coli, GI1787524, Length=243, Percent_Identity=32.5102880658436, Blast_Score=115, Evalue=2e-27,
Organism=Escherichia coli, GI1788510, Length=233, Percent_Identity=30.0429184549356, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI87081838, Length=189, Percent_Identity=30.6878306878307, Blast_Score=88, Evalue=5e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: NA

Molecular weight: Translated: 27878; Mature: 27878

Theoretical pI: Translated: 9.33; Mature: 9.33

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
7.0 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
7.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMIRIGGNMSERINKYLSAHGICSRREADRMIQEGRITIDGRIAAMGEMVDDNTIICVDG
CEEEECCCHHHHHHHHHHHCCCCCHHHHHHHHHCCCEEECCEEEEECCCCCCCEEEEEEC
RKVDNSTPPQVVIAFNKPVGIVCTTTDKQGKNNIVDFIGYKERIYPVGRLDKESDGLILL
EECCCCCCCEEEEEECCCEEEEEECCCCCCCCCHHHHCCCHHHCCCCCCCCCCCCCEEEE
TNDGDMMDRLLRSVNGHEKEYIVSVNKPINKDFIKRMSGGIYLKELDRTTRKCVVEKISD
ECCCHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHCCCEEEEHHHHHHHHHHHHHHCC
RTFRIILTQGLNRQIRRMCAECGYSVTKLTRIRIMNVELGDLKTGEYRELDGKELNTLYE
CEEEEEEECCCCHHHHHHHHHCCCCHHEEEEEEEEEEEECCCCCCCCCCCCCHHHHHHHH
KIYK
HHCC
>Mature Secondary Structure
MMIRIGGNMSERINKYLSAHGICSRREADRMIQEGRITIDGRIAAMGEMVDDNTIICVDG
CEEEECCCHHHHHHHHHHHCCCCCHHHHHHHHHCCCEEECCEEEEECCCCCCCEEEEEEC
RKVDNSTPPQVVIAFNKPVGIVCTTTDKQGKNNIVDFIGYKERIYPVGRLDKESDGLILL
EECCCCCCCEEEEEECCCEEEEEECCCCCCCCCHHHHCCCHHHCCCCCCCCCCCCCEEEE
TNDGDMMDRLLRSVNGHEKEYIVSVNKPINKDFIKRMSGGIYLKELDRTTRKCVVEKISD
ECCCHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHCCCEEEEHHHHHHHHHHHHHHCC
RTFRIILTQGLNRQIRRMCAECGYSVTKLTRIRIMNVELGDLKTGEYRELDGKELNTLYE
CEEEEEEECCCCHHHHHHHHHCCCCHHEEEEEEEEEEEECCCCCCCCCCCCCHHHHHHHH
KIYK
HHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]