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The map label for this gene is ligA [H]

Identifier: 238917130

GI number: 238917130

Start: 1212947

End: 1214956

Strand: Reverse

Name: ligA [H]

Synonym: EUBELI_01201

Alternate gene names: 238917130

Gene position: 1214956-1212947 (Counterclockwise)

Preceding gene: 238917131

Following gene: 238917129

Centisome position: 56.66

GC content: 39.35

Gene sequence:

>2010_bases
ATGGATTTATTACAGGAATATTCAGAACTTAAGAAGACGATAGATTATCATATGGACAGATATTACAATCAGGACGAGCC
GGAGATAAGCGATTATGAGTATGATAAGCTTATGATAAGGCTTAAAGAGATTGAGAAAGAACATCCGGAATGGGTGACAT
CTGATTCACCTTCACAGAAGATTGGTGGAACTGTCAAAAGAGAAGCCGGAGTTAAGGTCACACATAATGTACCTATGCTG
TCTATTGAAGATGTGTTTTCAGAAGATGCGGTGTGTGCATGGGTTGATAAGGTTCATGCCATGCATCCTGACTGCACATT
TTCAGTAGAGACTAAGATTGACGGTTTAAGCATGAGTTTAAGATATGTAAAAGAAGCAGATGGGAAGCTGCATTTGCATT
TGGCAGAGACAAGAGGAGATGGTCTTATTGGTGAGGATGTGACAGCTAATGCAAGGGTAGTGGGGGATGTGTTACAAGTT
ATTGACCTTCCTTATGATTCACTAGAACTTCGTGGCGAAGTGTATATGAGCCATGAAGATTTTGAAAAATATAATGAAGA
ACAGGAAAGACTTGAGAAAAAAACAGCTGCTAATCCCAGAAATCTTGCGGCCGGAACATTAAGACAGCTTGATACTTCAA
TTACGAAGAAGCGAGGTCTTAAGATATTTATCTTTAATGTACAGGACGGACCTGATGAGATTATGGAAAGCCACGGCAGG
GGAATGGATATATTAAAGGCAGCAGGTGTACCGGTTGTATATCATAAGATATGTAAGACTAAGGAAGAGGTTGTCAGAGC
AATTGATGAGATAGGAAGAATGAGAGAAGAACTTCCATATGACATTGACGGTGCTGTTGTAAAGATTGACCATATTGCCT
TCAGACAGGAGTTTCCAGCAGGAAGCAAGTATTCAAGCGGTCATATTGCATACAAATATCCGCCGGAAGAAAAGGTTATT
GTTATGGATGATATTCTTGTGGATGTGGGAAGAACAGGAAAACTTACATTTACAGGAATATTCCATGATCCAGAAACCGG
AAAGCCTGCCAGACTGTGTGGTACAAGTGTATCAAGAGCAACTCTTCATAATCAGGATTATATTACACAGATGAATGTAG
GAATTGGAGGAGAATATAAGCTGTTTAAGAGTGGTGAGATTATTCCTAAACTTAATGGCTGCGTAAAGGCACCTGAGCAT
GTGTTTGAAGCACCAAAGGTATGTCCTGTATGTGGAGAGCCTCTTATAAGAGAAGAGGATACAGCAGATATAAGATGCGT
CAATCCTTCATGTGCAGCACAGTTGTCAAGAACAGTAGCATATTTTACATCTCTTGACTGCATGAATATCATGGGGCTTG
GTGAAACTCTAGTGGATGCTCTCATAAAAGATGGTTATATACACAATTATGCTGATATATATGCATTGAAAGAACACCGT
GATGAACTTATTGAGAAGGGAATCATAGGAAAAGAGAAGAATACTGATAAGCTGCTTGCAGCTATTGAGAAATCTAAAGA
GAACACACCAGACAGACTGCTTACTGCACTTGGAATAAGAAATGTCGGCAAGAATACAGCCAAGCAGATTATGAATTATT
ATGATAATCTTATGGAACTTGCTGATGCAGGTGAGGAAGAACTGCAGAATATTCCGGATATAGGCGCAACTACAGCTAAA
TGTATACATGACTTTTTCCATGATGAGGCTAATATTGAATTGCTGGAAAGGCTAAAACAGTCAGGTCTTAATATGCAGAT
GCCAGAGAAGAAAGAAATGTCAGATAAGCTTGAAGGAAAGACGATTGTTGTAACAGGAAGCTTTGATAATTACAGCCGTA
AGGATATGGAAGAACTGATTGTTTCTAATGGAGGCAAAGCAACAGGAAGTGTCAGCAAAAAAACTGATTATCTAGTTGCA
GGTGAGGCAGCAGGCTCTAAGCTTGATAAGGCTAATTCACTGGGGGTTAAGGTGCTTACGATTGATGAATTTATGGAAAT
GATAAAGTAA

Upstream 100 bases:

>100_bases
AGCTTAATACACTTTATGAGAAGATATATAAGTAAGCAGTAAAAGCTTATATAAGCAGATTTTAATAGTCGATATATAAG
CGGCATATAAGGAGCGGATT

Downstream 100 bases:

>100_bases
AGAAAATTTTTATGCAGTTATTAAAGATGATATGATATAATCAGACAGCAATGATAACAGAAAGAAGGAACAGTAATGCC
AATTAAGATTCAGAGTGATT

Product: DNA ligase (NAD+)

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+] [H]

Number of amino acids: Translated: 669; Mature: 669

Protein sequence:

>669_residues
MDLLQEYSELKKTIDYHMDRYYNQDEPEISDYEYDKLMIRLKEIEKEHPEWVTSDSPSQKIGGTVKREAGVKVTHNVPML
SIEDVFSEDAVCAWVDKVHAMHPDCTFSVETKIDGLSMSLRYVKEADGKLHLHLAETRGDGLIGEDVTANARVVGDVLQV
IDLPYDSLELRGEVYMSHEDFEKYNEEQERLEKKTAANPRNLAAGTLRQLDTSITKKRGLKIFIFNVQDGPDEIMESHGR
GMDILKAAGVPVVYHKICKTKEEVVRAIDEIGRMREELPYDIDGAVVKIDHIAFRQEFPAGSKYSSGHIAYKYPPEEKVI
VMDDILVDVGRTGKLTFTGIFHDPETGKPARLCGTSVSRATLHNQDYITQMNVGIGGEYKLFKSGEIIPKLNGCVKAPEH
VFEAPKVCPVCGEPLIREEDTADIRCVNPSCAAQLSRTVAYFTSLDCMNIMGLGETLVDALIKDGYIHNYADIYALKEHR
DELIEKGIIGKEKNTDKLLAAIEKSKENTPDRLLTALGIRNVGKNTAKQIMNYYDNLMELADAGEEELQNIPDIGATTAK
CIHDFFHDEANIELLERLKQSGLNMQMPEKKEMSDKLEGKTIVVTGSFDNYSRKDMEELIVSNGGKATGSVSKKTDYLVA
GEAAGSKLDKANSLGVKVLTIDEFMEMIK

Sequences:

>Translated_669_residues
MDLLQEYSELKKTIDYHMDRYYNQDEPEISDYEYDKLMIRLKEIEKEHPEWVTSDSPSQKIGGTVKREAGVKVTHNVPML
SIEDVFSEDAVCAWVDKVHAMHPDCTFSVETKIDGLSMSLRYVKEADGKLHLHLAETRGDGLIGEDVTANARVVGDVLQV
IDLPYDSLELRGEVYMSHEDFEKYNEEQERLEKKTAANPRNLAAGTLRQLDTSITKKRGLKIFIFNVQDGPDEIMESHGR
GMDILKAAGVPVVYHKICKTKEEVVRAIDEIGRMREELPYDIDGAVVKIDHIAFRQEFPAGSKYSSGHIAYKYPPEEKVI
VMDDILVDVGRTGKLTFTGIFHDPETGKPARLCGTSVSRATLHNQDYITQMNVGIGGEYKLFKSGEIIPKLNGCVKAPEH
VFEAPKVCPVCGEPLIREEDTADIRCVNPSCAAQLSRTVAYFTSLDCMNIMGLGETLVDALIKDGYIHNYADIYALKEHR
DELIEKGIIGKEKNTDKLLAAIEKSKENTPDRLLTALGIRNVGKNTAKQIMNYYDNLMELADAGEEELQNIPDIGATTAK
CIHDFFHDEANIELLERLKQSGLNMQMPEKKEMSDKLEGKTIVVTGSFDNYSRKDMEELIVSNGGKATGSVSKKTDYLVA
GEAAGSKLDKANSLGVKVLTIDEFMEMIK
>Mature_669_residues
MDLLQEYSELKKTIDYHMDRYYNQDEPEISDYEYDKLMIRLKEIEKEHPEWVTSDSPSQKIGGTVKREAGVKVTHNVPML
SIEDVFSEDAVCAWVDKVHAMHPDCTFSVETKIDGLSMSLRYVKEADGKLHLHLAETRGDGLIGEDVTANARVVGDVLQV
IDLPYDSLELRGEVYMSHEDFEKYNEEQERLEKKTAANPRNLAAGTLRQLDTSITKKRGLKIFIFNVQDGPDEIMESHGR
GMDILKAAGVPVVYHKICKTKEEVVRAIDEIGRMREELPYDIDGAVVKIDHIAFRQEFPAGSKYSSGHIAYKYPPEEKVI
VMDDILVDVGRTGKLTFTGIFHDPETGKPARLCGTSVSRATLHNQDYITQMNVGIGGEYKLFKSGEIIPKLNGCVKAPEH
VFEAPKVCPVCGEPLIREEDTADIRCVNPSCAAQLSRTVAYFTSLDCMNIMGLGETLVDALIKDGYIHNYADIYALKEHR
DELIEKGIIGKEKNTDKLLAAIEKSKENTPDRLLTALGIRNVGKNTAKQIMNYYDNLMELADAGEEELQNIPDIGATTAK
CIHDFFHDEANIELLERLKQSGLNMQMPEKKEMSDKLEGKTIVVTGSFDNYSRKDMEELIVSNGGKATGSVSKKTDYLVA
GEAAGSKLDKANSLGVKVLTIDEFMEMIK

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain [H]

Homologues:

Organism=Escherichia coli, GI1788750, Length=681, Percent_Identity=36.7107195301028, Blast_Score=416, Evalue=1e-117,
Organism=Escherichia coli, GI87082305, Length=575, Percent_Identity=22.6086956521739, Blast_Score=107, Evalue=3e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001357
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149 [H]

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD [H]

EC number: =6.5.1.2 [H]

Molecular weight: Translated: 74879; Mature: 74879

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: PS50172 BRCT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLLQEYSELKKTIDYHMDRYYNQDEPEISDYEYDKLMIRLKEIEKEHPEWVTSDSPSQK
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHH
IGGTVKREAGVKVTHNVPMLSIEDVFSEDAVCAWVDKVHAMHPDCTFSVETKIDGLSMSL
HCCCEECCCCCEEECCCCEEEHHHHHCCCHHHHHHHHHHHCCCCCEEEEECCCCCHHHHH
RYVKEADGKLHLHLAETRGDGLIGEDVTANARVVGDVLQVIDLPYDSLELRGEVYMSHED
HHHHCCCCEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEECCEEEECHHH
FEKYNEEQERLEKKTAANPRNLAAGTLRQLDTSITKKRGLKIFIFNVQDGPDEIMESHGR
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHCCC
GMDILKAAGVPVVYHKICKTKEEVVRAIDEIGRMREELPYDIDGAVVKIDHIAFRQEFPA
CCHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHHCCCC
GSKYSSGHIAYKYPPEEKVIVMDDILVDVGRTGKLTFTGIFHDPETGKPARLCGTSVSRA
CCCCCCCEEEEECCCCCCEEEEEHHHHHCCCCCCEEEEEEEECCCCCCCHHHHCCCHHHH
TLHNQDYITQMNVGIGGEYKLFKSGEIIPKLNGCVKAPEHVFEAPKVCPVCGEPLIREED
HHCCCCCEEEEECCCCCCEEEEECCCCCCCCCCHHCCCHHHHCCCCCCCCCCCCCCCCCC
TADIRCVNPSCAAQLSRTVAYFTSLDCMNIMGLGETLVDALIKDGYIHNYADIYALKEHR
CCCEEEECHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHH
DELIEKGIIGKEKNTDKLLAAIEKSKENTPDRLLTALGIRNVGKNTAKQIMNYYDNLMEL
HHHHHCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
ADAGEEELQNIPDIGATTAKCIHDFFHDEANIELLERLKQSGLNMQMPEKKEMSDKLEGK
HCCCHHHHHCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHCCC
TIVVTGSFDNYSRKDMEELIVSNGGKATGSVSKKTDYLVAGEAAGSKLDKANSLGVKVLT
EEEEECCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCEEEEE
IDEFMEMIK
HHHHHHHHC
>Mature Secondary Structure
MDLLQEYSELKKTIDYHMDRYYNQDEPEISDYEYDKLMIRLKEIEKEHPEWVTSDSPSQK
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHH
IGGTVKREAGVKVTHNVPMLSIEDVFSEDAVCAWVDKVHAMHPDCTFSVETKIDGLSMSL
HCCCEECCCCCEEECCCCEEEHHHHHCCCHHHHHHHHHHHCCCCCEEEEECCCCCHHHHH
RYVKEADGKLHLHLAETRGDGLIGEDVTANARVVGDVLQVIDLPYDSLELRGEVYMSHED
HHHHCCCCEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEECCEEEECHHH
FEKYNEEQERLEKKTAANPRNLAAGTLRQLDTSITKKRGLKIFIFNVQDGPDEIMESHGR
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHCCC
GMDILKAAGVPVVYHKICKTKEEVVRAIDEIGRMREELPYDIDGAVVKIDHIAFRQEFPA
CCHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHHCCCC
GSKYSSGHIAYKYPPEEKVIVMDDILVDVGRTGKLTFTGIFHDPETGKPARLCGTSVSRA
CCCCCCCEEEEECCCCCCEEEEEHHHHHCCCCCCEEEEEEEECCCCCCCHHHHCCCHHHH
TLHNQDYITQMNVGIGGEYKLFKSGEIIPKLNGCVKAPEHVFEAPKVCPVCGEPLIREED
HHCCCCCEEEEECCCCCCEEEEECCCCCCCCCCHHCCCHHHHCCCCCCCCCCCCCCCCCC
TADIRCVNPSCAAQLSRTVAYFTSLDCMNIMGLGETLVDALIKDGYIHNYADIYALKEHR
CCCEEEECHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHH
DELIEKGIIGKEKNTDKLLAAIEKSKENTPDRLLTALGIRNVGKNTAKQIMNYYDNLMEL
HHHHHCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
ADAGEEELQNIPDIGATTAKCIHDFFHDEANIELLERLKQSGLNMQMPEKKEMSDKLEGK
HCCCHHHHHCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHCCC
TIVVTGSFDNYSRKDMEELIVSNGGKATGSVSKKTDYLVAGEAAGSKLDKANSLGVKVLT
EEEEECCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCEEEEE
IDEFMEMIK
HHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA