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The map label for this gene is pyk [H]

Identifier: 238917063

GI number: 238917063

Start: 1144575

End: 1146038

Strand: Reverse

Name: pyk [H]

Synonym: EUBELI_01133

Alternate gene names: 238917063

Gene position: 1146038-1144575 (Counterclockwise)

Preceding gene: 238917064

Following gene: 238917062

Centisome position: 53.45

GC content: 38.8

Gene sequence:

>1464_bases
GTGTTTGAAAAGATTTTAATTATTTTGGAGGAAACAATGAAAAAGACTAAGATTATTTGCACAATGGGTCCTAATACTAA
CGACAGAAACCTTATTAAGGATCTTGCGTTAGCAGGAATGGATATTGCAAGATTTAATTTTTCTCATGGTGACCATGAGG
AGCAGGCAGGAAGATTTGCCTTAATTAAAAGTGTAAGAGAAGAACTTAATATCCCTATTGCAACTCTTCTTGATACTAAG
GGACCTGAGATTAGAACAGGTGCTGTTAAGGATGACAAGAAGGTTACACTTGTTGAGGGACAGAAATATACATTAACAAC
AAGACAGGTGCCAGCAGATGATAAGATTAATATGGTAACTTATGCAGGTCTTCCAGAAGATGTTACTACAGGTAATATTA
TTCTTATCGATGATGGTCTTATCGAGCTTAAGGTTGATAAGGTTGAAGGTACAGAGATTGAATGTACAGTAATTAATGGT
GGTGAGCTTGGTTCTAAGAAGGGTGTTAATGTTCCTAACGTAAGCATCAGACTTCCAGGCATCACAGAGAAAGATAAGGA
AGATATCATCTTTGGTGTTGAGCAGGGATTTGATTTTATCGCAGCTTCATTTGTAAGAAATGCAGCATGTATCGAGGAAA
TTAAGCACCTTCTCTGGGAGCATGGTTCAGATATCCCAGTTATTGCTAAGATTGAGAATGCCGAAGGTATTGCTAATATA
GATGATATCATCAGAGTTGCTGATGGTATCATGGTAGCCCGTGGCGATATGGGTGTTGAGATTCCTGCTGAGGAAGTTCC
ACATGTTCAGAAGGAAATCATCAAGAAGTGTAATGCTAAGTATAAGCCAGTTATCACAGCTACACAGATGCTTGATTCTA
TGATCAGAAATCCAAGACCTACAAGAGCTGAGGTTACTGATGTTGCTAACGCTATCTACGACGGAACAGATGTTGTTATG
CTTAGTGGTGAGACTGCTAATGGAAAGTACCCATTAGAGGCTGTTAAGATGATGGTTAAGATTGCAGAAAGAACAGAGCG
GGAGATTAAAGGACATTCAGTAGAATATTCTAATCTTCACAGCAAGAGAAGCATTTCAAGCGCTGTATGTAATGCTGCAG
TTCAGACAGCAGATAACCTTGGAGCTAAGGCTATTATCTGTCCTACAATTTCAGGATTTACAGCAAGACTTACATCTAAG
TTAAAGCCAGAAGCTGAGATTATCGGATGCTCTCCATACGATAACGTTCTTAGAAAGATGCAGATTTACTGGGGTGTAAG
ACCTCTTAAGACTGCTACAGAAGCTTCTACTGATAAGATTATTGAGCATGCATTAGTAGTTTCTGAACATGCTGGATTCG
TAGAAGAAGGAGATACAGCAATTGTTTCTGCTGGTATTGCTACATCTTCTGATCCTTCATCTAAGAGAGGACTTACTAAC
ACAATGAGAGTAGTTACAATCTAG

Upstream 100 bases:

>100_bases
TATACCCACATCCATGTAACTGGTGATAAATAAGGTAAAGCTTGTTAAATAAAAAACTTAGTTGATTTGACACGCAAATA
TGTTATTATATTGTAGGTAT

Downstream 100 bases:

>100_bases
TGTTAATTTGATACATTTGTTTCGGAATGAGGCTTGATTTTAATATTCTATTATGTTATAAGTAATACACAATTAATATT
ACAAAGCGTTGATGGAAACA

Product: pyruvate kinase

Products: NA

Alternate protein names: PK [H]

Number of amino acids: Translated: 487; Mature: 487

Protein sequence:

>487_residues
MFEKILIILEETMKKTKIICTMGPNTNDRNLIKDLALAGMDIARFNFSHGDHEEQAGRFALIKSVREELNIPIATLLDTK
GPEIRTGAVKDDKKVTLVEGQKYTLTTRQVPADDKINMVTYAGLPEDVTTGNIILIDDGLIELKVDKVEGTEIECTVING
GELGSKKGVNVPNVSIRLPGITEKDKEDIIFGVEQGFDFIAASFVRNAACIEEIKHLLWEHGSDIPVIAKIENAEGIANI
DDIIRVADGIMVARGDMGVEIPAEEVPHVQKEIIKKCNAKYKPVITATQMLDSMIRNPRPTRAEVTDVANAIYDGTDVVM
LSGETANGKYPLEAVKMMVKIAERTEREIKGHSVEYSNLHSKRSISSAVCNAAVQTADNLGAKAIICPTISGFTARLTSK
LKPEAEIIGCSPYDNVLRKMQIYWGVRPLKTATEASTDKIIEHALVVSEHAGFVEEGDTAIVSAGIATSSDPSSKRGLTN
TMRVVTI

Sequences:

>Translated_487_residues
MFEKILIILEETMKKTKIICTMGPNTNDRNLIKDLALAGMDIARFNFSHGDHEEQAGRFALIKSVREELNIPIATLLDTK
GPEIRTGAVKDDKKVTLVEGQKYTLTTRQVPADDKINMVTYAGLPEDVTTGNIILIDDGLIELKVDKVEGTEIECTVING
GELGSKKGVNVPNVSIRLPGITEKDKEDIIFGVEQGFDFIAASFVRNAACIEEIKHLLWEHGSDIPVIAKIENAEGIANI
DDIIRVADGIMVARGDMGVEIPAEEVPHVQKEIIKKCNAKYKPVITATQMLDSMIRNPRPTRAEVTDVANAIYDGTDVVM
LSGETANGKYPLEAVKMMVKIAERTEREIKGHSVEYSNLHSKRSISSAVCNAAVQTADNLGAKAIICPTISGFTARLTSK
LKPEAEIIGCSPYDNVLRKMQIYWGVRPLKTATEASTDKIIEHALVVSEHAGFVEEGDTAIVSAGIATSSDPSSKRGLTN
TMRVVTI
>Mature_487_residues
MFEKILIILEETMKKTKIICTMGPNTNDRNLIKDLALAGMDIARFNFSHGDHEEQAGRFALIKSVREELNIPIATLLDTK
GPEIRTGAVKDDKKVTLVEGQKYTLTTRQVPADDKINMVTYAGLPEDVTTGNIILIDDGLIELKVDKVEGTEIECTVING
GELGSKKGVNVPNVSIRLPGITEKDKEDIIFGVEQGFDFIAASFVRNAACIEEIKHLLWEHGSDIPVIAKIENAEGIANI
DDIIRVADGIMVARGDMGVEIPAEEVPHVQKEIIKKCNAKYKPVITATQMLDSMIRNPRPTRAEVTDVANAIYDGTDVVM
LSGETANGKYPLEAVKMMVKIAERTEREIKGHSVEYSNLHSKRSISSAVCNAAVQTADNLGAKAIICPTISGFTARLTSK
LKPEAEIIGCSPYDNVLRKMQIYWGVRPLKTATEASTDKIIEHALVVSEHAGFVEEGDTAIVSAGIATSSDPSSKRGLTN
TMRVVTI

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI33286418, Length=497, Percent_Identity=41.6498993963783, Blast_Score=350, Evalue=1e-96,
Organism=Homo sapiens, GI33286422, Length=497, Percent_Identity=41.2474849094567, Blast_Score=348, Evalue=6e-96,
Organism=Homo sapiens, GI33286420, Length=497, Percent_Identity=41.2474849094567, Blast_Score=348, Evalue=6e-96,
Organism=Homo sapiens, GI10835121, Length=495, Percent_Identity=41.010101010101, Blast_Score=337, Evalue=2e-92,
Organism=Homo sapiens, GI32967597, Length=495, Percent_Identity=41.010101010101, Blast_Score=336, Evalue=3e-92,
Organism=Homo sapiens, GI310128732, Length=289, Percent_Identity=43.2525951557093, Blast_Score=225, Evalue=9e-59,
Organism=Homo sapiens, GI310128730, Length=289, Percent_Identity=43.2525951557093, Blast_Score=225, Evalue=9e-59,
Organism=Homo sapiens, GI310128736, Length=237, Percent_Identity=40.5063291139241, Blast_Score=172, Evalue=6e-43,
Organism=Homo sapiens, GI310128734, Length=237, Percent_Identity=40.5063291139241, Blast_Score=172, Evalue=6e-43,
Organism=Homo sapiens, GI310128738, Length=211, Percent_Identity=41.2322274881517, Blast_Score=154, Evalue=2e-37,
Organism=Escherichia coli, GI1787965, Length=474, Percent_Identity=46.8354430379747, Blast_Score=417, Evalue=1e-118,
Organism=Escherichia coli, GI1788160, Length=491, Percent_Identity=36.4562118126273, Blast_Score=283, Evalue=2e-77,
Organism=Caenorhabditis elegans, GI17544584, Length=486, Percent_Identity=43.2098765432099, Blast_Score=370, Evalue=1e-102,
Organism=Caenorhabditis elegans, GI71984406, Length=490, Percent_Identity=42.0408163265306, Blast_Score=331, Evalue=6e-91,
Organism=Caenorhabditis elegans, GI17506829, Length=490, Percent_Identity=42.0408163265306, Blast_Score=331, Evalue=7e-91,
Organism=Caenorhabditis elegans, GI71984413, Length=490, Percent_Identity=42.0408163265306, Blast_Score=330, Evalue=7e-91,
Organism=Caenorhabditis elegans, GI17506831, Length=490, Percent_Identity=42.0408163265306, Blast_Score=329, Evalue=2e-90,
Organism=Saccharomyces cerevisiae, GI6319279, Length=493, Percent_Identity=40.3651115618661, Blast_Score=322, Evalue=9e-89,
Organism=Saccharomyces cerevisiae, GI6324923, Length=492, Percent_Identity=38.2113821138211, Blast_Score=315, Evalue=1e-86,
Organism=Drosophila melanogaster, GI28571814, Length=489, Percent_Identity=41.9222903885481, Blast_Score=361, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24648964, Length=489, Percent_Identity=41.9222903885481, Blast_Score=361, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24648966, Length=407, Percent_Identity=46.4373464373464, Blast_Score=336, Evalue=2e-92,
Organism=Drosophila melanogaster, GI24581235, Length=487, Percent_Identity=32.4435318275154, Blast_Score=247, Evalue=1e-65,
Organism=Drosophila melanogaster, GI24646914, Length=271, Percent_Identity=43.1734317343173, Blast_Score=197, Evalue=1e-50,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 53081; Mature: 53081

Theoretical pI: Translated: 5.20; Mature: 5.20

Prosite motif: PS00110 PYRUVATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFEKILIILEETMKKTKIICTMGPNTNDRNLIKDLALAGMDIARFNFSHGDHEEQAGRFA
CCHHHHHHHHHHHHHEEEEEEECCCCCHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHH
LIKSVREELNIPIATLLDTKGPEIRTGAVKDDKKVTLVEGQKYTLTTRQVPADDKINMVT
HHHHHHHHHCCCEEEEECCCCCCEEECCCCCCCEEEEEECCEEEEEECCCCCCCCEEEEE
YAGLPEDVTTGNIILIDDGLIELKVDKVEGTEIECTVINGGELGSKKGVNVPNVSIRLPG
ECCCCCCCCCCCEEEEECCEEEEEEEECCCCEEEEEEEECCCCCCCCCCCCCCEEEECCC
ITEKDKEDIIFGVEQGFDFIAASFVRNAACIEEIKHLLWEHGSDIPVIAKIENAEGIANI
CCCCCCCCEEEEHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCH
DDIIRVADGIMVARGDMGVEIPAEEVPHVQKEIIKKCNAKYKPVITATQMLDSMIRNPRP
HHHHHHHCCEEEEECCCCCCCCHHHCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCC
TRAEVTDVANAIYDGTDVVMLSGETANGKYPLEAVKMMVKIAERTEREIKGHSVEYSNLH
CCHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEHHHHH
SKRSISSAVCNAAVQTADNLGAKAIICPTISGFTARLTSKLKPEAEIIGCSPYDNVLRKM
HHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHCCCCCCEEEECCCHHHHHHHH
QIYWGVRPLKTATEASTDKIIEHALVVSEHAGFVEEGDTAIVSAGIATSSDPSSKRGLTN
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCCCCCCC
TMRVVTI
CEEEEEC
>Mature Secondary Structure
MFEKILIILEETMKKTKIICTMGPNTNDRNLIKDLALAGMDIARFNFSHGDHEEQAGRFA
CCHHHHHHHHHHHHHEEEEEEECCCCCHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHH
LIKSVREELNIPIATLLDTKGPEIRTGAVKDDKKVTLVEGQKYTLTTRQVPADDKINMVT
HHHHHHHHHCCCEEEEECCCCCCEEECCCCCCCEEEEEECCEEEEEECCCCCCCCEEEEE
YAGLPEDVTTGNIILIDDGLIELKVDKVEGTEIECTVINGGELGSKKGVNVPNVSIRLPG
ECCCCCCCCCCCEEEEECCEEEEEEEECCCCEEEEEEEECCCCCCCCCCCCCCEEEECCC
ITEKDKEDIIFGVEQGFDFIAASFVRNAACIEEIKHLLWEHGSDIPVIAKIENAEGIANI
CCCCCCCCEEEEHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCH
DDIIRVADGIMVARGDMGVEIPAEEVPHVQKEIIKKCNAKYKPVITATQMLDSMIRNPRP
HHHHHHHCCEEEEECCCCCCCCHHHCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCC
TRAEVTDVANAIYDGTDVVMLSGETANGKYPLEAVKMMVKIAERTEREIKGHSVEYSNLH
CCHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEHHHHH
SKRSISSAVCNAAVQTADNLGAKAIICPTISGFTARLTSKLKPEAEIIGCSPYDNVLRKM
HHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHCCCCCCEEEECCCHHHHHHHH
QIYWGVRPLKTATEASTDKIIEHALVVSEHAGFVEEGDTAIVSAGIATSSDPSSKRGLTN
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCCCCCCC
TMRVVTI
CEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11466286; 9625784 [H]