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The map label for this gene is rimO [H]

Identifier: 238917054

GI number: 238917054

Start: 1133854

End: 1135206

Strand: Reverse

Name: rimO [H]

Synonym: EUBELI_01124

Alternate gene names: 238917054

Gene position: 1135206-1133854 (Counterclockwise)

Preceding gene: 238917056

Following gene: 238917053

Centisome position: 52.94

GC content: 39.32

Gene sequence:

>1353_bases
ATGCAGGAGGTAATGCAGTATATGAAGATTATGTTTGCATCTTTAGGTTGTGATAAAAACCTTGTAGATACAGAGAATAT
GCTTGGAATCCTTAACGATAAGGGCTTTGAGTTTACAGATGATGAAACACAGGCAGATGTTATTGTTGTCAATACATGCT
GTTTTATTGGTGATGCCAAGCAGGAGAGTATCAACACGATACTTGAGATGGCACAGCACAAGGAAGATGCTGTGTGTAAG
GCACTTATTGTTACAGGATGCCTTGCTCACAGATATAAGGATGAGATTATCAAAGAGATTCCGGAGGTTGATGCATTTTT
AGGAACAACTTCTTATGACAAGATTGCGGAGGTTGTTACTTCAGTGCTTGAAGGTAAGGGCTTTAATGTCGTTGATGATG
CCAACAGACTTCCTATTGTTAAAGAAAAGAGAATTATTACTACGCCGGGATATTTTGAGTACCTTAAGATAGCGGAAGGA
TGCGATAAGCATTGTACATACTGTATAATTCCTAAGGTTCGTGGCAATTTCAGAAGTTATCCTGTTGAGTATCTTGTGGA
GCAGGCAAAGCACCTTGTACACAATGGAGCAAAAGAGCTTATACTTGTAGCACAGGAGACAACTCTTTATGGAACAGACC
TTTACGGAAAGAAATCACTTCCAATGCTTATACATGAGCTTGCCAAGATTGAAGACCTCAAATGGATAAGAATTCAGTAC
TGTTATCCGGAAGAGATTAATGATGAGCTTATCGAGGCTATTAAGAATGAGCCTAAAGTCTGTCATTATCTTGATATGCC
AATCCAGCATGCAAGTGATAATGTATTAAAGCGCATGGGAAGAAAGACAGATAAGCAGGAGCTTCTTGATATTGTTGCGA
AGCTGAGAAAAGAGATTCCGGACATTGCATTAAGAACAACTCTTATTGCCGGCTTCCCGGGAGAGACACAGGCTGACCAT
GAAGAGGTTATGGAATTTATTGATGAAATTGAGTTTGACAGACTTGGTGTATTTACTTATTCAAGAGAAGAGGATACTCC
TGCTGCAACAATGCCGGACCAGATTGACCAGGATATAATGGATACATGGAGAGACGAGCTTATGGCTCTGCAACAGGAAA
TATCTATTGACAAGTCAGCACAAATGGTTGGAAAGACTATTGATGTTATGGTTGAAGGCTATATTGCAGAGGACAATACT
TATGTCGGACGTTCGTACAAGGATGCACCTAATGTTGATGGAATGGTGTTCTTTGAATGTGACAGGGAACTTATGTCAGG
AGATTTTGTATCTGTAAAGATTACCAGTTCAACTGAATATGATTTAATGGGAATAATGGAGGAAAATGTTTAA

Upstream 100 bases:

>100_bases
AGGGCTTATTAAAAGTGCCAGTTTGATAATGTTTTATATGGTCAGATTATGTAAGGGATATGTTTACATTTGCAAAAAAT
GTGTTACAATGAGGAGTACT

Downstream 100 bases:

>100_bases
ATGAATTTACCTAACAAACTTACAATTGCAAGAGTTATTATGATTCCTTTGTTTCTGATATGTCTGTATCTTAACATTGG
ATGTGGCAAATATATTGCAG

Product: 2-alkenal reductase

Products: NA

Alternate protein names: S12 MTTase; S12 methylthiotransferase; Ribosome maturation factor RimO [H]

Number of amino acids: Translated: 450; Mature: 450

Protein sequence:

>450_residues
MQEVMQYMKIMFASLGCDKNLVDTENMLGILNDKGFEFTDDETQADVIVVNTCCFIGDAKQESINTILEMAQHKEDAVCK
ALIVTGCLAHRYKDEIIKEIPEVDAFLGTTSYDKIAEVVTSVLEGKGFNVVDDANRLPIVKEKRIITTPGYFEYLKIAEG
CDKHCTYCIIPKVRGNFRSYPVEYLVEQAKHLVHNGAKELILVAQETTLYGTDLYGKKSLPMLIHELAKIEDLKWIRIQY
CYPEEINDELIEAIKNEPKVCHYLDMPIQHASDNVLKRMGRKTDKQELLDIVAKLRKEIPDIALRTTLIAGFPGETQADH
EEVMEFIDEIEFDRLGVFTYSREEDTPAATMPDQIDQDIMDTWRDELMALQQEISIDKSAQMVGKTIDVMVEGYIAEDNT
YVGRSYKDAPNVDGMVFFECDRELMSGDFVSVKITSSTEYDLMGIMEENV

Sequences:

>Translated_450_residues
MQEVMQYMKIMFASLGCDKNLVDTENMLGILNDKGFEFTDDETQADVIVVNTCCFIGDAKQESINTILEMAQHKEDAVCK
ALIVTGCLAHRYKDEIIKEIPEVDAFLGTTSYDKIAEVVTSVLEGKGFNVVDDANRLPIVKEKRIITTPGYFEYLKIAEG
CDKHCTYCIIPKVRGNFRSYPVEYLVEQAKHLVHNGAKELILVAQETTLYGTDLYGKKSLPMLIHELAKIEDLKWIRIQY
CYPEEINDELIEAIKNEPKVCHYLDMPIQHASDNVLKRMGRKTDKQELLDIVAKLRKEIPDIALRTTLIAGFPGETQADH
EEVMEFIDEIEFDRLGVFTYSREEDTPAATMPDQIDQDIMDTWRDELMALQQEISIDKSAQMVGKTIDVMVEGYIAEDNT
YVGRSYKDAPNVDGMVFFECDRELMSGDFVSVKITSSTEYDLMGIMEENV
>Mature_450_residues
MQEVMQYMKIMFASLGCDKNLVDTENMLGILNDKGFEFTDDETQADVIVVNTCCFIGDAKQESINTILEMAQHKEDAVCK
ALIVTGCLAHRYKDEIIKEIPEVDAFLGTTSYDKIAEVVTSVLEGKGFNVVDDANRLPIVKEKRIITTPGYFEYLKIAEG
CDKHCTYCIIPKVRGNFRSYPVEYLVEQAKHLVHNGAKELILVAQETTLYGTDLYGKKSLPMLIHELAKIEDLKWIRIQY
CYPEEINDELIEAIKNEPKVCHYLDMPIQHASDNVLKRMGRKTDKQELLDIVAKLRKEIPDIALRTTLIAGFPGETQADH
EEVMEFIDEIEFDRLGVFTYSREEDTPAATMPDQIDQDIMDTWRDELMALQQEISIDKSAQMVGKTIDVMVEGYIAEDNT
YVGRSYKDAPNVDGMVFFECDRELMSGDFVSVKITSSTEYDLMGIMEENV

Specific function: Catalyzes the methylthiolation of an aspartic acid residue of ribosomal protein S12 [H]

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI28872784, Length=483, Percent_Identity=26.2939958592132, Blast_Score=169, Evalue=5e-42,
Organism=Homo sapiens, GI28872782, Length=483, Percent_Identity=26.2939958592132, Blast_Score=169, Evalue=5e-42,
Organism=Homo sapiens, GI93277076, Length=367, Percent_Identity=29.700272479564, Blast_Score=159, Evalue=5e-39,
Organism=Escherichia coli, GI1787057, Length=454, Percent_Identity=35.9030837004405, Blast_Score=285, Evalue=3e-78,
Organism=Escherichia coli, GI1786882, Length=449, Percent_Identity=29.1759465478842, Blast_Score=184, Evalue=1e-47,
Organism=Caenorhabditis elegans, GI17553146, Length=427, Percent_Identity=25.7611241217799, Blast_Score=150, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI71996771, Length=256, Percent_Identity=34.765625, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI21356207, Length=479, Percent_Identity=24.6346555323591, Blast_Score=165, Evalue=7e-41,
Organism=Drosophila melanogaster, GI19922432, Length=457, Percent_Identity=27.3522975929978, Blast_Score=155, Evalue=5e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR012340
- InterPro:   IPR007197
- InterPro:   IPR005840
- InterPro:   IPR002792 [H]

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004 [H]

EC number: NA

Molecular weight: Translated: 51231; Mature: 51231

Theoretical pI: Translated: 4.31; Mature: 4.31

Prosite motif: PS50926 TRAM ; PS01278 UPF0004

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
6.7 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
6.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQEVMQYMKIMFASLGCDKNLVDTENMLGILNDKGFEFTDDETQADVIVVNTCCFIGDAK
CHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEEECCCCH
QESINTILEMAQHKEDAVCKALIVTGCLAHRYKDEIIKEIPEVDAFLGTTSYDKIAEVVT
HHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCHHHHHHHHH
SVLEGKGFNVVDDANRLPIVKEKRIITTPGYFEYLKIAEGCDKHCTYCIIPKVRGNFRSY
HHHCCCCCCEECCCCCCCCEECCEEEECCCHHHHHHHHHCCCCCCCEEEEECCCCCCCCC
PVEYLVEQAKHLVHNGAKELILVAQETTLYGTDLYGKKSLPMLIHELAKIEDLKWIRIQY
CHHHHHHHHHHHHHCCCCCEEEEEECCEEEECCCCCCCCHHHHHHHHHHHCCCEEEEEEE
CYPEEINDELIEAIKNEPKVCHYLDMPIQHASDNVLKRMGRKTDKQELLDIVAKLRKEIP
ECCHHCCHHHHHHHHCCCCEEEEECCCHHHCCHHHHHHHCCCCCHHHHHHHHHHHHHHCC
DIALRTTLIAGFPGETQADHEEVMEFIDEIEFDRLGVFTYSREEDTPAATMPDQIDQDIM
HHHHHHHHEECCCCCCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCHHHHHHHH
DTWRDELMALQQEISIDKSAQMVGKTIDVMVEGYIAEDNTYVGRSYKDAPNVDGMVFFEC
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHEEEEEEECCCCEECCCCCCCCCCCCEEEEEE
DRELMSGDFVSVKITSSTEYDLMGIMEENV
CHHHHCCCEEEEEEECCCCCHHHHHHCCCC
>Mature Secondary Structure
MQEVMQYMKIMFASLGCDKNLVDTENMLGILNDKGFEFTDDETQADVIVVNTCCFIGDAK
CHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEEECCCCH
QESINTILEMAQHKEDAVCKALIVTGCLAHRYKDEIIKEIPEVDAFLGTTSYDKIAEVVT
HHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCHHHHHHHHH
SVLEGKGFNVVDDANRLPIVKEKRIITTPGYFEYLKIAEGCDKHCTYCIIPKVRGNFRSY
HHHCCCCCCEECCCCCCCCEECCEEEECCCHHHHHHHHHCCCCCCCEEEEECCCCCCCCC
PVEYLVEQAKHLVHNGAKELILVAQETTLYGTDLYGKKSLPMLIHELAKIEDLKWIRIQY
CHHHHHHHHHHHHHCCCCCEEEEEECCEEEECCCCCCCCHHHHHHHHHHHCCCEEEEEEE
CYPEEINDELIEAIKNEPKVCHYLDMPIQHASDNVLKRMGRKTDKQELLDIVAKLRKEIP
ECCHHCCHHHHHHHHCCCCEEEEECCCHHHCCHHHHHHHCCCCCHHHHHHHHHHHHHHCC
DIALRTTLIAGFPGETQADHEEVMEFIDEIEFDRLGVFTYSREEDTPAATMPDQIDQDIM
HHHHHHHHEECCCCCCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCHHHHHHHH
DTWRDELMALQQEISIDKSAQMVGKTIDVMVEGYIAEDNTYVGRSYKDAPNVDGMVFFEC
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHEEEEEEECCCCEECCCCCCCCCCCCEEEEEE
DRELMSGDFVSVKITSSTEYDLMGIMEENV
CHHHHCCCEEEEEEECCCCCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA