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The map label for this gene is ppnK

Identifier: 238917012

GI number: 238917012

Start: 1095766

End: 1096647

Strand: Reverse

Name: ppnK

Synonym: EUBELI_01082

Alternate gene names: 238917012

Gene position: 1096647-1095766 (Counterclockwise)

Preceding gene: 238917013

Following gene: 238917011

Centisome position: 51.15

GC content: 37.3

Gene sequence:

>882_bases
ATGAAATATTTTTATATTGTTACTAACAGGTTTAAAGATCCAGATGGTGTTAATACAAGAAAGATTGCACATTTTTTACG
CTCAAAAGGAGCTGAATGTGTATGCCAGATAGAACAGGAAAAGGCATTTAATAAAACAGGTTCATACAGTGATGTAAGAC
TGGTTCCTAATAATACTGAATGTGTTATTGTTCTTGGAGGTGATGGTACACTTATTCAGGCATCAAGGGAGTTGTCTGAG
AAGGATATTCCTTTTATAGGCGTTAACATCGGAACTCTTGGTTATCTTACAGACACAGATATGTCAAGCTTTGAGGAGAC
ATTAGAGAGTCTTCTTAGAGACGATTATGAGATTGACAGACGCATGATGCTTGATGGATGTATATATCGCGGTGAAGAAC
GCATTTTTTCTGATATGGCACTTAATGATGTTGTAATTAACAGAAATGGTGCTTTAAGAATTATTGATTTTGATATATAT
GTCAATGGCGAGTATCTTAATACATATTCGGCTGACGGGGTAATTGTTTCGACCGCAACAGGATCGACAGCGTACAGTCT
GTCAGCAGGAGGACCTATAATACAGCCGACAGCAAGACTTATAATGGTGACACCTATATGTCCGCATTCACTTAATCAGA
GAAGTATTATATTTGCTGCTGATGATGAGATTATGATTGAGATGAAGGATAATAAAAGCTCATCAGGAAGAATGACGGGT
TCTCTTAAGAATGACAGCGCAAGAGTTGCCACATTTGATGGAGAAAGCTTCTGTGAAGTTGTAACGGGAGACAGAATTGT
TATTACGCAGTCAGAGCGTATATCAAGATTTGTCAAGACAAGCAGAATTTCATTCTTAGAGAGAATAAGGAACAAAATGT
AG

Upstream 100 bases:

>100_bases
ATAAAGGAAATTCCATTTGTTGTAGAAAATGTAGTTGAAAAAGCTCACGAAGTATTGTAATATATATTTAATTCTTTTCG
TATACTAAATGAGGTTCAGC

Downstream 100 bases:

>100_bases
TTGGGGATAATTATGAAAACAGACAGACATAAAAAGATATTAGAAGTAATTGACAGATACGAAGTTGGAACACAGGAGGA
GCTGGCAAAGATTCTGAACG

Product: NAD+ kinase

Products: NA

Alternate protein names: Poly(P)/ATP NAD kinase

Number of amino acids: Translated: 293; Mature: 293

Protein sequence:

>293_residues
MKYFYIVTNRFKDPDGVNTRKIAHFLRSKGAECVCQIEQEKAFNKTGSYSDVRLVPNNTECVIVLGGDGTLIQASRELSE
KDIPFIGVNIGTLGYLTDTDMSSFEETLESLLRDDYEIDRRMMLDGCIYRGEERIFSDMALNDVVINRNGALRIIDFDIY
VNGEYLNTYSADGVIVSTATGSTAYSLSAGGPIIQPTARLIMVTPICPHSLNQRSIIFAADDEIMIEMKDNKSSSGRMTG
SLKNDSARVATFDGESFCEVVTGDRIVITQSERISRFVKTSRISFLERIRNKM

Sequences:

>Translated_293_residues
MKYFYIVTNRFKDPDGVNTRKIAHFLRSKGAECVCQIEQEKAFNKTGSYSDVRLVPNNTECVIVLGGDGTLIQASRELSE
KDIPFIGVNIGTLGYLTDTDMSSFEETLESLLRDDYEIDRRMMLDGCIYRGEERIFSDMALNDVVINRNGALRIIDFDIY
VNGEYLNTYSADGVIVSTATGSTAYSLSAGGPIIQPTARLIMVTPICPHSLNQRSIIFAADDEIMIEMKDNKSSSGRMTG
SLKNDSARVATFDGESFCEVVTGDRIVITQSERISRFVKTSRISFLERIRNKM
>Mature_293_residues
MKYFYIVTNRFKDPDGVNTRKIAHFLRSKGAECVCQIEQEKAFNKTGSYSDVRLVPNNTECVIVLGGDGTLIQASRELSE
KDIPFIGVNIGTLGYLTDTDMSSFEETLESLLRDDYEIDRRMMLDGCIYRGEERIFSDMALNDVVINRNGALRIIDFDIY
VNGEYLNTYSADGVIVSTATGSTAYSLSAGGPIIQPTARLIMVTPICPHSLNQRSIIFAADDEIMIEMKDNKSSSGRMTG
SLKNDSARVATFDGESFCEVVTGDRIVITQSERISRFVKTSRISFLERIRNKM

Specific function: Catalyzes the phosphorylation of NAD to NADP. Utilizes ATP and other nucleoside triphosphates as well as inorganic polyphosphate as a source of phosphorus

COG id: COG0061

COG function: function code G; Predicted sugar kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD kinase family

Homologues:

Organism=Homo sapiens, GI55743112, Length=241, Percent_Identity=32.7800829875519, Blast_Score=99, Evalue=7e-21,
Organism=Escherichia coli, GI1788968, Length=291, Percent_Identity=28.5223367697594, Blast_Score=131, Evalue=6e-32,
Organism=Saccharomyces cerevisiae, GI6320794, Length=249, Percent_Identity=33.7349397590361, Blast_Score=134, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6322509, Length=249, Percent_Identity=30.5220883534137, Blast_Score=123, Evalue=3e-29,
Organism=Saccharomyces cerevisiae, GI6325068, Length=186, Percent_Identity=33.3333333333333, Blast_Score=115, Evalue=8e-27,
Organism=Drosophila melanogaster, GI28573832, Length=228, Percent_Identity=34.6491228070175, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI28573830, Length=228, Percent_Identity=34.6491228070175, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI161077047, Length=228, Percent_Identity=34.6491228070175, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI28573828, Length=228, Percent_Identity=34.6491228070175, Blast_Score=109, Evalue=2e-24,
Organism=Drosophila melanogaster, GI28573826, Length=228, Percent_Identity=34.6491228070175, Blast_Score=109, Evalue=2e-24,
Organism=Drosophila melanogaster, GI281363323, Length=253, Percent_Identity=30.0395256916996, Blast_Score=97, Evalue=9e-21,
Organism=Drosophila melanogaster, GI20129957, Length=253, Percent_Identity=30.0395256916996, Blast_Score=97, Evalue=9e-21,
Organism=Drosophila melanogaster, GI281363321, Length=253, Percent_Identity=30.0395256916996, Blast_Score=97, Evalue=9e-21,
Organism=Drosophila melanogaster, GI24653422, Length=253, Percent_Identity=30.0395256916996, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24653424, Length=253, Percent_Identity=30.0395256916996, Blast_Score=97, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PPNK_EUBE2 (C4Z0G9)

Other databases:

- EMBL:   CP001104
- RefSeq:   YP_002930529.1
- GeneID:   7957389
- GenomeReviews:   CP001104_GR
- KEGG:   eel:EUBELI_01082
- OMA:   FLERIRN
- ProtClustDB:   CLSK2506265
- GO:   GO:0005737
- HAMAP:   MF_00361
- InterPro:   IPR016064
- InterPro:   IPR017438
- InterPro:   IPR017437
- InterPro:   IPR002504
- Gene3D:   G3DSA:2.60.200.30
- Gene3D:   G3DSA:3.40.50.10330
- PANTHER:   PTHR20275

Pfam domain/function: PF01513 NAD_kinase; SSF111331 ATP-NAD_kinase_PpnK-typ

EC number: =2.7.1.23

Molecular weight: Translated: 32754; Mature: 32754

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYFYIVTNRFKDPDGVNTRKIAHFLRSKGAECVCQIEQEKAFNKTGSYSDVRLVPNNTE
CEEEEEEEECCCCCCCCCHHHHHHHHHCCCCEEEEEEHHHHCCCCCCCCCEEEEECCCCE
CVIVLGGDGTLIQASRELSEKDIPFIGVNIGTLGYLTDTDMSSFEETLESLLRDDYEIDR
EEEEECCCCCEEEECHHCCCCCCCEEEEEECEEEEEECCCHHHHHHHHHHHHHCCHHHHH
RMMLDGCIYRGEERIFSDMALNDVVINRNGALRIIDFDIYVNGEYLNTYSADGVIVSTAT
HHHHHHHHCCCHHHHHHHCCEEEEEEECCCEEEEEEEEEEECCEEEEEECCCCEEEEECC
GSTAYSLSAGGPIIQPTARLIMVTPICPHSLNQRSIIFAADDEIMIEMKDNKSSSGRMTG
CCEEEEECCCCCEECCCCEEEEEECCCCCCCCCCEEEEEECCEEEEEECCCCCCCCEEEE
SLKNDSARVATFDGESFCEVVTGDRIVITQSERISRFVKTSRISFLERIRNKM
EECCCCEEEEEECCCCEEHEECCCEEEEECHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKYFYIVTNRFKDPDGVNTRKIAHFLRSKGAECVCQIEQEKAFNKTGSYSDVRLVPNNTE
CEEEEEEEECCCCCCCCCHHHHHHHHHCCCCEEEEEEHHHHCCCCCCCCCEEEEECCCCE
CVIVLGGDGTLIQASRELSEKDIPFIGVNIGTLGYLTDTDMSSFEETLESLLRDDYEIDR
EEEEECCCCCEEEECHHCCCCCCCEEEEEECEEEEEECCCHHHHHHHHHHHHHCCHHHHH
RMMLDGCIYRGEERIFSDMALNDVVINRNGALRIIDFDIYVNGEYLNTYSADGVIVSTAT
HHHHHHHHCCCHHHHHHHCCEEEEEEECCCEEEEEEEEEEECCEEEEEECCCCEEEEECC
GSTAYSLSAGGPIIQPTARLIMVTPICPHSLNQRSIIFAADDEIMIEMKDNKSSSGRMTG
CCEEEEECCCCCEECCCCEEEEEECCCCCCCCCCEEEEEECCEEEEEECCCCCCCCEEEE
SLKNDSARVATFDGESFCEVVTGDRIVITQSERISRFVKTSRISFLERIRNKM
EECCCCEEEEEECCCCEEHEECCCEEEEECHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA