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The map label for this gene is proB [H]

Identifier: 238916914

GI number: 238916914

Start: 999177

End: 1000028

Strand: Direct

Name: proB [H]

Synonym: EUBELI_00983

Alternate gene names: 238916914

Gene position: 999177-1000028 (Clockwise)

Preceding gene: 238916913

Following gene: 238916915

Centisome position: 46.6

GC content: 37.56

Gene sequence:

>852_bases
ATGGGGAATTATAGAGAGTTTATAAAAGACAAGAAAAGAATTGTTATAAAGATAGGTTCATCTTCACTTATGCATGAGCA
GACAGGCAGATTGGACCTTTTAAAGATTGAAAAGCTTGTAAGGATTCTTATTGATATTAAGAATTCAGGAAAAGATGTTG
TTTTAGTTTCTTCTGGTGCAATAGCTGTGGGAAGGGCTGTTATAGGACTTTGTGACAGACCTACAGAACTTCCGGTAAAA
CAGGCATGTGCATCCATAGGACAGGCTAAGTTAATGATGGTTTACCAGAAGATATTTGCAGAATATGGTGCAGTTGCATC
ACAGGTTCTTCTTACCAAGAACACAATAGTTAATGATGTTAACAGGACTAATGCACAGAATACATTTAATGAAATCTTAA
AGCTTGGGGCAGTTCCAATCGTTAATGAGAACGATACAGTATCAACTTACGAGATTGAACAGCTACAGGCTTTTGGAGAT
AATGACAGACTTTCAGCAATTGTTGCTTCAATTATCGGAGCGGACCTTCTTATTCTGTTATCTGATATTGATGGTCTGTA
TACAGATGATCCAAACACTAATCCTAATGCAAAATTTGTTGAAACTGTATACGAGATTGATGAGAAGCTTATGGGAATGG
GTAAAGATTCATCCGGCAGTAATATGGGGACTGGCGGAATGACAACCAAGCTTATTGCAGGAAAGATTGCAACACTGTCG
GGGGCGGATATGATTATTACTAATGGTAATGATGTTGACAATATTATCAGGGTTATGGCTGGGGAAAATGTTGGAACATT
GTTCTTAGACCACAGAGCATCTGATTTTGACCTGATGTCTCTTATAGATTAA

Upstream 100 bases:

>100_bases
TATGGCAGTAACATCTGCTTGTATGGGATAATATGGACTTTTTAATAAAAATATAGTAAAATAGACCAGCATTTGATTTT
TTTACTGGAGATATTAAAAT

Downstream 100 bases:

>100_bases
GGCCGATTGAAGATTACTTGGACGACAACAGGAGGTCATACATGGATATTAAGCTGATGAAGAATTCGGAGAAGGGTATA
TTTTTTCCTGTCAATGACAG

Product: glutamate 5-kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase; GK [H]

Number of amino acids: Translated: 283; Mature: 282

Protein sequence:

>283_residues
MGNYREFIKDKKRIVIKIGSSSLMHEQTGRLDLLKIEKLVRILIDIKNSGKDVVLVSSGAIAVGRAVIGLCDRPTELPVK
QACASIGQAKLMMVYQKIFAEYGAVASQVLLTKNTIVNDVNRTNAQNTFNEILKLGAVPIVNENDTVSTYEIEQLQAFGD
NDRLSAIVASIIGADLLILLSDIDGLYTDDPNTNPNAKFVETVYEIDEKLMGMGKDSSGSNMGTGGMTTKLIAGKIATLS
GADMIITNGNDVDNIIRVMAGENVGTLFLDHRASDFDLMSLID

Sequences:

>Translated_283_residues
MGNYREFIKDKKRIVIKIGSSSLMHEQTGRLDLLKIEKLVRILIDIKNSGKDVVLVSSGAIAVGRAVIGLCDRPTELPVK
QACASIGQAKLMMVYQKIFAEYGAVASQVLLTKNTIVNDVNRTNAQNTFNEILKLGAVPIVNENDTVSTYEIEQLQAFGD
NDRLSAIVASIIGADLLILLSDIDGLYTDDPNTNPNAKFVETVYEIDEKLMGMGKDSSGSNMGTGGMTTKLIAGKIATLS
GADMIITNGNDVDNIIRVMAGENVGTLFLDHRASDFDLMSLID
>Mature_282_residues
GNYREFIKDKKRIVIKIGSSSLMHEQTGRLDLLKIEKLVRILIDIKNSGKDVVLVSSGAIAVGRAVIGLCDRPTELPVKQ
ACASIGQAKLMMVYQKIFAEYGAVASQVLLTKNTIVNDVNRTNAQNTFNEILKLGAVPIVNENDTVSTYEIEQLQAFGDN
DRLSAIVASIIGADLLILLSDIDGLYTDDPNTNPNAKFVETVYEIDEKLMGMGKDSSGSNMGTGGMTTKLIAGKIATLSG
ADMIITNGNDVDNIIRVMAGENVGTLFLDHRASDFDLMSLID

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline [H]

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Homo sapiens, GI62912457, Length=293, Percent_Identity=31.740614334471, Blast_Score=134, Evalue=6e-32,
Organism=Homo sapiens, GI21361368, Length=295, Percent_Identity=31.864406779661, Blast_Score=132, Evalue=4e-31,
Organism=Escherichia coli, GI1786437, Length=261, Percent_Identity=40.9961685823755, Blast_Score=185, Evalue=2e-48,
Organism=Caenorhabditis elegans, GI17569967, Length=280, Percent_Identity=34.2857142857143, Blast_Score=147, Evalue=6e-36,
Organism=Caenorhabditis elegans, GI17569969, Length=280, Percent_Identity=34.2857142857143, Blast_Score=147, Evalue=6e-36,
Organism=Saccharomyces cerevisiae, GI6321822, Length=247, Percent_Identity=44.5344129554656, Blast_Score=206, Evalue=5e-54,
Organism=Saccharomyces cerevisiae, GI6320506, Length=258, Percent_Identity=43.7984496124031, Blast_Score=196, Evalue=2e-51,
Organism=Drosophila melanogaster, GI21357643, Length=293, Percent_Identity=33.7883959044369, Blast_Score=144, Evalue=1e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797
- InterPro:   IPR002478
- InterPro:   IPR015947 [H]

Pfam domain/function: PF00696 AA_kinase; PF01472 PUA [H]

EC number: =2.7.2.11 [H]

Molecular weight: Translated: 30604; Mature: 30473

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: PS00902 GLUTAMATE_5_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGNYREFIKDKKRIVIKIGSSSLMHEQTGRLDLLKIEKLVRILIDIKNSGKDVVLVSSGA
CCCHHHHHCCCCEEEEEECCCHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCC
IAVGRAVIGLCDRPTELPVKQACASIGQAKLMMVYQKIFAEYGAVASQVLLTKNTIVNDV
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
NRTNAQNTFNEILKLGAVPIVNENDTVSTYEIEQLQAFGDNDRLSAIVASIIGADLLILL
CCCCHHHHHHHHHHHCCCEEECCCCCEEHEEHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
SDIDGLYTDDPNTNPNAKFVETVYEIDEKLMGMGKDSSGSNMGTGGMTTKLIAGKIATLS
HHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHEEEC
GADMIITNGNDVDNIIRVMAGENVGTLFLDHRASDFDLMSLID
CCEEEEECCCCHHHHHHHHCCCCCCEEEEECCCCCCHHHHHCC
>Mature Secondary Structure 
GNYREFIKDKKRIVIKIGSSSLMHEQTGRLDLLKIEKLVRILIDIKNSGKDVVLVSSGA
CCHHHHHCCCCEEEEEECCCHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCC
IAVGRAVIGLCDRPTELPVKQACASIGQAKLMMVYQKIFAEYGAVASQVLLTKNTIVNDV
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
NRTNAQNTFNEILKLGAVPIVNENDTVSTYEIEQLQAFGDNDRLSAIVASIIGADLLILL
CCCCHHHHHHHHHHHCCCEEECCCCCEEHEEHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
SDIDGLYTDDPNTNPNAKFVETVYEIDEKLMGMGKDSSGSNMGTGGMTTKLIAGKIATLS
HHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHEEEC
GADMIITNGNDVDNIIRVMAGENVGTLFLDHRASDFDLMSLID
CCEEEEECCCCHHHHHHHHCCCCCCEEEEECCCCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA