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The map label for this gene is pnp [H]

Identifier: 238916832

GI number: 238916832

Start: 910335

End: 912506

Strand: Direct

Name: pnp [H]

Synonym: EUBELI_00900

Alternate gene names: 238916832

Gene position: 910335-912506 (Clockwise)

Preceding gene: 238916831

Following gene: 238916833

Centisome position: 42.46

GC content: 40.06

Gene sequence:

>2172_bases
ATGTATATCATGGCGGTAGTTTATGGTGTATTTTTCAGTTGTTTCGGACCTTCTGTTAAATATATATTTTTTATGGAAGG
AGTTCATATGTTTAAACAGTATGAAATGGAACTCGCCGGAAGAACTTTAAGAGTTGATATCGGCAGAGTGTGTGCACAGG
CTAACGGTGCTGCATTAATGCATTATGGTGATACTGTTGTTCTTTCAACAGCAACAGCATCTAAAGAACCAAGAGAAGGA
ATTGATTTCTTCCCATTAAGCGTTGAATATGAAGAGAAGATGTATGCAGCAGGAAAGATTCCTGGTGGATTTAACAAGAG
AGAAGGTAAGGCATCTGAGAATGCTATCCTTACATCAAGAGTTATCGACAGACCTATGAGACCATTATTCCCTAAGGACT
ATCGTAACGATGTTACTCTTAATAACATGGTTATGTCAGTTGATGAGAATTGCAGACCAGAATTACTTGCTATGATTGGT
TCTGCTATTGCAACATCTATTTCTGATATTCCATTTGACGGTCCATGTGCTACAACACAGATTGGTATGATTGATGGAGA
ATTCATCGTTAACCCATCTCAGGCACAGTGGCAGGATGGAGATCTTCAGCTTACAGTTGCTTCAACTAAGCAGAAGGTTA
TTATGATTGAAGCAGGAGCTAATGAGATTCCAGAAGATAAGATGATTGAGGCTATATATAAGGCTCATGATATCAACCAG
ACAATCATTGCATTTATTGATAAGATTGTTGCTGAGGTTGGCAAGGAAAAGCATTCTTATGTAAGCTGTGCTGTTCCTGC
AGAGATGTTTGAGGCTATGAAGCAGGTTGTATCTCCAGAAGAGATGGAAGTTGCTGTATTCACAGATGACAAGCAGACAA
GAGAGAAGAATATTGATGCTGTAACTGAGAAAATGAAGGAAGCATTTGCAGATAATGAGGAATGGCTTGCAGTTCTTGGT
GAAGCTGTTTACCAGTACCAGAAGAAGACTGTTCGTAAGATGATCTTAAAGGATCACAAGAGACCAGACGGAAGAGCCAT
TAACCAGATCAGACCACTTGCAGCTGAGGTTGATATCATTCCTAGAGTTCATGGTTCTGCAATGTTTACAAGAGGACAGA
CACAGATCTGTGATGTTGTTACTCTTGCACCTTTATCAGAGGCACAGAAGGTTGATGGTCTTGATGAGAATGTAACTACT
AAGAGATACATACATCACTATAATTTCCCTTCATACTCTGTAGGTGAGACTAAGCCATCAAGAGGACCAGGACGTCGTGA
AATCGGACATGGAGCATTAGCAGAGAAGGCACTTGTTCCTGTACTTCCATCAGAGGAAGAGTTCCCATATGCAATCCGTG
CCGTTTCTGAGACATTTGAATCTAATGGTTCTACATCAATGGCTTCAACATGTGCATCTTGTATGTCACTTATGGCAGCA
GGTGTTCCTATTAAGAGAATGGTAGCCGGTATTTCATGTGGTCTTGTTACAGGTGAGACAGATGATGATTATATTGTACT
TACTGATATCCAGGGACTTGAAGATTTCTTTGGAGATATGGATTTCAAGGTAACAGGTACAACAGAAGGTATTACAGCTA
TCCAGATGGATATTAAGATTCACGGTCTTACAAGACCAATCGTTGAAGAGGCTATAAGAAGAACAAGAGAAGCAAGACTT
TTCATCATGGATACATGTATGAAGCCTGCTATTGCTGAGCCTAGAAAGACTGTTGGTGAGTATGCGCCTAAGATTATCCA
GACATCTATTGATCCTGCTAAGATTGGCGAAGTAGTTGGACAGCGTGGTAAGACAATCAACGCAATCATTGATGAGTGCG
GTGTCAAGATTGATATTACTGATGATGGATTCGTTTCTGTATGTGGTGTTGAGCAGGCAGGAATGGATAAGGCTATGAAG
TATATCAAGACTATTGTAGAAGACTTCGAAGAAGGCAAGATATATGAAGGTAAGGTTGTAAGCATTAAGGAATTCGGTGC
CTTCGTAGAGTTTGCTCCTGGCAAGGAAGGAATGGTTCACATTTCTAAGATTTCTAATGAGAGAATCAACCATGTTGAAG
ATGTTCTTACACTTGGCGATCATGTAAAGTGCAAGTGTATGGGTAAGGATAAGATGGGAAGAATCAGCTTCTCAATTAAG
GATGCAATGTAA

Upstream 100 bases:

>100_bases
TTGAGCGTTTAGGCTTAAGAAAGTAATTAATCAGGGCGGAGTGTATAACTCCGCCCTATTGCATTGTTAATGGCAGAAGG
GATACCCCGAGACCACTGAA

Downstream 100 bases:

>100_bases
AAGCATGAGTTATATTTGGTTATAGAAAAGAGGATACGATTATGGTAGATCAGAATTGTGCATATTGTGTAGAGGGTGAC
CTCGTAGCTAAGTTTGGTAT

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase [H]

Number of amino acids: Translated: 723; Mature: 723

Protein sequence:

>723_residues
MYIMAVVYGVFFSCFGPSVKYIFFMEGVHMFKQYEMELAGRTLRVDIGRVCAQANGAALMHYGDTVVLSTATASKEPREG
IDFFPLSVEYEEKMYAAGKIPGGFNKREGKASENAILTSRVIDRPMRPLFPKDYRNDVTLNNMVMSVDENCRPELLAMIG
SAIATSISDIPFDGPCATTQIGMIDGEFIVNPSQAQWQDGDLQLTVASTKQKVIMIEAGANEIPEDKMIEAIYKAHDINQ
TIIAFIDKIVAEVGKEKHSYVSCAVPAEMFEAMKQVVSPEEMEVAVFTDDKQTREKNIDAVTEKMKEAFADNEEWLAVLG
EAVYQYQKKTVRKMILKDHKRPDGRAINQIRPLAAEVDIIPRVHGSAMFTRGQTQICDVVTLAPLSEAQKVDGLDENVTT
KRYIHHYNFPSYSVGETKPSRGPGRREIGHGALAEKALVPVLPSEEEFPYAIRAVSETFESNGSTSMASTCASCMSLMAA
GVPIKRMVAGISCGLVTGETDDDYIVLTDIQGLEDFFGDMDFKVTGTTEGITAIQMDIKIHGLTRPIVEEAIRRTREARL
FIMDTCMKPAIAEPRKTVGEYAPKIIQTSIDPAKIGEVVGQRGKTINAIIDECGVKIDITDDGFVSVCGVEQAGMDKAMK
YIKTIVEDFEEGKIYEGKVVSIKEFGAFVEFAPGKEGMVHISKISNERINHVEDVLTLGDHVKCKCMGKDKMGRISFSIK
DAM

Sequences:

>Translated_723_residues
MYIMAVVYGVFFSCFGPSVKYIFFMEGVHMFKQYEMELAGRTLRVDIGRVCAQANGAALMHYGDTVVLSTATASKEPREG
IDFFPLSVEYEEKMYAAGKIPGGFNKREGKASENAILTSRVIDRPMRPLFPKDYRNDVTLNNMVMSVDENCRPELLAMIG
SAIATSISDIPFDGPCATTQIGMIDGEFIVNPSQAQWQDGDLQLTVASTKQKVIMIEAGANEIPEDKMIEAIYKAHDINQ
TIIAFIDKIVAEVGKEKHSYVSCAVPAEMFEAMKQVVSPEEMEVAVFTDDKQTREKNIDAVTEKMKEAFADNEEWLAVLG
EAVYQYQKKTVRKMILKDHKRPDGRAINQIRPLAAEVDIIPRVHGSAMFTRGQTQICDVVTLAPLSEAQKVDGLDENVTT
KRYIHHYNFPSYSVGETKPSRGPGRREIGHGALAEKALVPVLPSEEEFPYAIRAVSETFESNGSTSMASTCASCMSLMAA
GVPIKRMVAGISCGLVTGETDDDYIVLTDIQGLEDFFGDMDFKVTGTTEGITAIQMDIKIHGLTRPIVEEAIRRTREARL
FIMDTCMKPAIAEPRKTVGEYAPKIIQTSIDPAKIGEVVGQRGKTINAIIDECGVKIDITDDGFVSVCGVEQAGMDKAMK
YIKTIVEDFEEGKIYEGKVVSIKEFGAFVEFAPGKEGMVHISKISNERINHVEDVLTLGDHVKCKCMGKDKMGRISFSIK
DAM
>Mature_723_residues
MYIMAVVYGVFFSCFGPSVKYIFFMEGVHMFKQYEMELAGRTLRVDIGRVCAQANGAALMHYGDTVVLSTATASKEPREG
IDFFPLSVEYEEKMYAAGKIPGGFNKREGKASENAILTSRVIDRPMRPLFPKDYRNDVTLNNMVMSVDENCRPELLAMIG
SAIATSISDIPFDGPCATTQIGMIDGEFIVNPSQAQWQDGDLQLTVASTKQKVIMIEAGANEIPEDKMIEAIYKAHDINQ
TIIAFIDKIVAEVGKEKHSYVSCAVPAEMFEAMKQVVSPEEMEVAVFTDDKQTREKNIDAVTEKMKEAFADNEEWLAVLG
EAVYQYQKKTVRKMILKDHKRPDGRAINQIRPLAAEVDIIPRVHGSAMFTRGQTQICDVVTLAPLSEAQKVDGLDENVTT
KRYIHHYNFPSYSVGETKPSRGPGRREIGHGALAEKALVPVLPSEEEFPYAIRAVSETFESNGSTSMASTCASCMSLMAA
GVPIKRMVAGISCGLVTGETDDDYIVLTDIQGLEDFFGDMDFKVTGTTEGITAIQMDIKIHGLTRPIVEEAIRRTREARL
FIMDTCMKPAIAEPRKTVGEYAPKIIQTSIDPAKIGEVVGQRGKTINAIIDECGVKIDITDDGFVSVCGVEQAGMDKAMK
YIKTIVEDFEEGKIYEGKVVSIKEFGAFVEFAPGKEGMVHISKISNERINHVEDVLTLGDHVKCKCMGKDKMGRISFSIK
DAM

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction [H]

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Homo sapiens, GI188528628, Length=715, Percent_Identity=36.2237762237762, Blast_Score=416, Evalue=1e-116,
Organism=Escherichia coli, GI145693187, Length=683, Percent_Identity=41.7276720351391, Blast_Score=546, Evalue=1e-156,
Organism=Caenorhabditis elegans, GI115534063, Length=737, Percent_Identity=32.0217096336499, Blast_Score=325, Evalue=5e-89,
Organism=Caenorhabditis elegans, GI17535281, Length=75, Percent_Identity=50.6666666666667, Blast_Score=70, Evalue=6e-12,
Organism=Drosophila melanogaster, GI281362905, Length=698, Percent_Identity=36.5329512893983, Blast_Score=428, Evalue=1e-120,
Organism=Drosophila melanogaster, GI24651641, Length=698, Percent_Identity=36.5329512893983, Blast_Score=428, Evalue=1e-120,
Organism=Drosophila melanogaster, GI24651643, Length=698, Percent_Identity=36.5329512893983, Blast_Score=428, Evalue=1e-120,
Organism=Drosophila melanogaster, GI161079377, Length=643, Percent_Identity=35.7698289269051, Blast_Score=388, Evalue=1e-108,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967 [H]

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1 [H]

EC number: =2.7.7.8 [H]

Molecular weight: Translated: 79842; Mature: 79842

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS50084 KH_TYPE_1 ; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYIMAVVYGVFFSCFGPSVKYIFFMEGVHMFKQYEMELAGRTLRVDIGRVCAQANGAALM
CCHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHCCCEEEEEHHHHHHCCCCCEEE
HYGDTVVLSTATASKEPREGIDFFPLSVEYEEKMYAAGKIPGGFNKREGKASENAILTSR
EECCEEEEEECCCCCCCCCCCCEEEEEEEHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHH
VIDRPMRPLFPKDYRNDVTLNNMVMSVDENCRPELLAMIGSAIATSISDIPFDGPCATTQ
HHHCCCCCCCCCCCCCCCCHHHEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEE
IGMIDGEFIVNPSQAQWQDGDLQLTVASTKQKVIMIEAGANEIPEDKMIEAIYKAHDINQ
EEEECCCEEECCCCCCCCCCCEEEEEECCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCH
TIIAFIDKIVAEVGKEKHSYVSCAVPAEMFEAMKQVVSPEEMEVAVFTDDKQTREKNIDA
HHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHCCCCCEEEEEEECCHHHHHHHHHH
VTEKMKEAFADNEEWLAVLGEAVYQYQKKTVRKMILKDHKRPDGRAINQIRPLAAEVDII
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCHHHHEEEE
PRVHGSAMFTRGQTQICDVVTLAPLSEAQKVDGLDENVTTKRYIHHYNFPSYSVGETKPS
CCCCCCEEEECCCCHHHHHEEECCCHHHHHCCCCCCCCCHHHHHEECCCCCCCCCCCCCC
RGPGRREIGHGALAEKALVPVLPSEEEFPYAIRAVSETFESNGSTSMASTCASCMSLMAA
CCCCCHHCCCCHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHC
GVPIKRMVAGISCGLVTGETDDDYIVLTDIQGLEDFFGDMDFKVTGTTEGITAIQMDIKI
CCCHHHHHHHHHEEEEECCCCCCEEEEEECCHHHHHHCCCCEEEECCCCCEEEEEEEEEE
HGLTRPIVEEAIRRTREARLFIMDTCMKPAIAEPRKTVGEYAPKIIQTSIDPAKIGEVVG
ECCCHHHHHHHHHHHHHCEEEEEHHHCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHH
QRGKTINAIIDECGVKIDITDDGFVSVCGVEQAGMDKAMKYIKTIVEDFEEGKIYEGKVV
CCCCCHHHHHHHCCCEEEECCCCCEEEECCCCCCHHHHHHHHHHHHHHHHCCCEECCEEE
SIKEFGAFVEFAPGKEGMVHISKISNERINHVEDVLTLGDHVKCKCMGKDKMGRISFSIK
EHHHHCCEEEECCCCCCCEEEEHHCCHHHHHHHHHHHCCCCEEEEEECCCCCCEEEEEEE
DAM
CCC
>Mature Secondary Structure
MYIMAVVYGVFFSCFGPSVKYIFFMEGVHMFKQYEMELAGRTLRVDIGRVCAQANGAALM
CCHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHCCCEEEEEHHHHHHCCCCCEEE
HYGDTVVLSTATASKEPREGIDFFPLSVEYEEKMYAAGKIPGGFNKREGKASENAILTSR
EECCEEEEEECCCCCCCCCCCCEEEEEEEHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHH
VIDRPMRPLFPKDYRNDVTLNNMVMSVDENCRPELLAMIGSAIATSISDIPFDGPCATTQ
HHHCCCCCCCCCCCCCCCCHHHEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEE
IGMIDGEFIVNPSQAQWQDGDLQLTVASTKQKVIMIEAGANEIPEDKMIEAIYKAHDINQ
EEEECCCEEECCCCCCCCCCCEEEEEECCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCH
TIIAFIDKIVAEVGKEKHSYVSCAVPAEMFEAMKQVVSPEEMEVAVFTDDKQTREKNIDA
HHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHCCCCCEEEEEEECCHHHHHHHHHH
VTEKMKEAFADNEEWLAVLGEAVYQYQKKTVRKMILKDHKRPDGRAINQIRPLAAEVDII
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCHHHHEEEE
PRVHGSAMFTRGQTQICDVVTLAPLSEAQKVDGLDENVTTKRYIHHYNFPSYSVGETKPS
CCCCCCEEEECCCCHHHHHEEECCCHHHHHCCCCCCCCCHHHHHEECCCCCCCCCCCCCC
RGPGRREIGHGALAEKALVPVLPSEEEFPYAIRAVSETFESNGSTSMASTCASCMSLMAA
CCCCCHHCCCCHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHC
GVPIKRMVAGISCGLVTGETDDDYIVLTDIQGLEDFFGDMDFKVTGTTEGITAIQMDIKI
CCCHHHHHHHHHEEEEECCCCCCEEEEEECCHHHHHHCCCCEEEECCCCCEEEEEEEEEE
HGLTRPIVEEAIRRTREARLFIMDTCMKPAIAEPRKTVGEYAPKIIQTSIDPAKIGEVVG
ECCCHHHHHHHHHHHHHCEEEEEHHHCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHH
QRGKTINAIIDECGVKIDITDDGFVSVCGVEQAGMDKAMKYIKTIVEDFEEGKIYEGKVV
CCCCCHHHHHHHCCCEEEECCCCCEEEECCCCCCHHHHHHHHHHHHHHHHCCCEECCEEE
SIKEFGAFVEFAPGKEGMVHISKISNERINHVEDVLTLGDHVKCKCMGKDKMGRISFSIK
EHHHHCCEEEECCCCCCCEEEEHHCCHHHHHHHHHHHCCCCEEEEEECCCCCCEEEEEEE
DAM
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA