Click here to switch to the map view.

The map label for this gene is fliM [H]

Identifier: 238916760

GI number: 238916760

Start: 836896

End: 837879

Strand: Direct

Name: fliM [H]

Synonym: EUBELI_00822

Alternate gene names: 238916760

Gene position: 836896-837879 (Clockwise)

Preceding gene: 238916759

Following gene: 238916761

Centisome position: 39.03

GC content: 35.98

Gene sequence:

>984_bases
ATGGGAGAGGTTCTTTCACAGAGTGAAATTGATAACCTCTTGAAAGCTCTCAGCAGCGGAGAATTAGATGTTGATGAAAT
GAAGAATACAGAAGAAAAGCAGGTCAAGAATTATGATTTTGCAAGACCTTCTAAGTTTTCTAAAGAACATTTAAGAACTC
TTGAGATTATATTTGAACATTTTGGCAGACTTCTGGCAACGAATCTTCCTGCTTACCTCAGAAAATCGGTGAGTGTTGAT
GTCGTTAACTCAGAAGTTGTTATATATTCAGAATTTTCTAATGCACTTTCTAATCCTGTATTGCTTGGAGTTGTAGGAAT
GCAGCCGTTGTCAGGCAATATAATTATGGAAATGGCTTCAAATCTTGGATTTGCCATTGTTGACAGATTGCTTGGTGGTG
CAGGTAATTCGCTTGATAAAGAAAGAGATTTTTCAGAAATAGAGCTTTCTATACTGGAAAGAATATTTACAGTCTGCGTT
AATCTTATGCAGGAACCATGGGAGAATGTAGTTAAGGTTAATCCAAGACTGGAAAGAATTGAAACTAATTCACAATTTGC
CCAGATTATTTCTCCGAGTGAGACAATTGCGATTGTAACCATTAATTTAAAGATTGGTGACGTTGAGGGACTTGTGAACA
TATGTCTGCCATTTGCTACATTAGAGCCGGTAATGGATAAGCTCAATACCAAATACTGGTTTTCTAATATGCAGGAGAAA
GATACTAATACATATGAAGATGCAATTGAGACTGTTATTGATAAGGCAATGATTCCGGTAAAGGCAGTGCTGGGAACATC
TAAAATCAGTGTTTCAGACTTTGCGAATCTTCAGATTGGCGATATAATAAGGATTGATAAAAAAGTCGATCAGGAACTGG
AAGTGTTTGTTGGAAATATTAAAAAGTTTAAGGCTCTTCCGGGATATTACGAAGATAAATATGCAGTAAGAGTTACAGAA
GTAATTAGAAAGGAGAGTGAATAA

Upstream 100 bases:

>100_bases
GCAGGAATTATTTAAGACAGAGACGATTCAATCGGTTCTTGTTAATATGGTTATTCAGTAATGATGATATATACAATAAT
TATTAAAGGAGGTGAGGAAG

Downstream 100 bases:

>100_bases
GAATGGATGGAATGTTATCCCAGGACGAGATAAATGCACTTCTTAGTGGAATGGGGTCAGGAGGAGATGATGCTGAATCA
ACAGGTACAGCTACTGTGAC

Product: flagellar motor switch protein FliM

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 327; Mature: 326

Protein sequence:

>327_residues
MGEVLSQSEIDNLLKALSSGELDVDEMKNTEEKQVKNYDFARPSKFSKEHLRTLEIIFEHFGRLLATNLPAYLRKSVSVD
VVNSEVVIYSEFSNALSNPVLLGVVGMQPLSGNIIMEMASNLGFAIVDRLLGGAGNSLDKERDFSEIELSILERIFTVCV
NLMQEPWENVVKVNPRLERIETNSQFAQIISPSETIAIVTINLKIGDVEGLVNICLPFATLEPVMDKLNTKYWFSNMQEK
DTNTYEDAIETVIDKAMIPVKAVLGTSKISVSDFANLQIGDIIRIDKKVDQELEVFVGNIKKFKALPGYYEDKYAVRVTE
VIRKESE

Sequences:

>Translated_327_residues
MGEVLSQSEIDNLLKALSSGELDVDEMKNTEEKQVKNYDFARPSKFSKEHLRTLEIIFEHFGRLLATNLPAYLRKSVSVD
VVNSEVVIYSEFSNALSNPVLLGVVGMQPLSGNIIMEMASNLGFAIVDRLLGGAGNSLDKERDFSEIELSILERIFTVCV
NLMQEPWENVVKVNPRLERIETNSQFAQIISPSETIAIVTINLKIGDVEGLVNICLPFATLEPVMDKLNTKYWFSNMQEK
DTNTYEDAIETVIDKAMIPVKAVLGTSKISVSDFANLQIGDIIRIDKKVDQELEVFVGNIKKFKALPGYYEDKYAVRVTE
VIRKESE
>Mature_326_residues
GEVLSQSEIDNLLKALSSGELDVDEMKNTEEKQVKNYDFARPSKFSKEHLRTLEIIFEHFGRLLATNLPAYLRKSVSVDV
VNSEVVIYSEFSNALSNPVLLGVVGMQPLSGNIIMEMASNLGFAIVDRLLGGAGNSLDKERDFSEIELSILERIFTVCVN
LMQEPWENVVKVNPRLERIETNSQFAQIISPSETIAIVTINLKIGDVEGLVNICLPFATLEPVMDKLNTKYWFSNMQEKD
TNTYEDAIETVIDKAMIPVKAVLGTSKISVSDFANLQIGDIIRIDKKVDQELEVFVGNIKKFKALPGYYEDKYAVRVTEV
IRKESE

Specific function: FliM is one of three proteins (FliG, FliN, FliM) that form the rotor-mounted switch complex (C ring), located at the base of the basal body. This complex interacts with the CheY and CheZ chemotaxis proteins, in addition to contacting components of the mot

COG id: COG1868

COG function: function code N; Flagellar motor switch protein

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein (Potential). Bacterial flagellum basal body [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FliM family [H]

Homologues:

Organism=Escherichia coli, GI1788255, Length=326, Percent_Identity=26.6871165644172, Blast_Score=112, Evalue=4e-26,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001689
- InterPro:   IPR001543 [H]

Pfam domain/function: PF02154 FliM; PF01052 SpoA [H]

EC number: NA

Molecular weight: Translated: 36737; Mature: 36605

Theoretical pI: Translated: 4.49; Mature: 4.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGEVLSQSEIDNLLKALSSGELDVDEMKNTEEKQVKNYDFARPSKFSKEHLRTLEIIFEH
CCCCCCHHHHHHHHHHHHCCCCCHHHHCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
FGRLLATNLPAYLRKSVSVDVVNSEVVIYSEFSNALSNPVLLGVVGMQPLSGNIIMEMAS
HHHHHHHCCHHHHHHCCEEEEECCCEEEEECHHHHHCCCEEEEEECCCCCCCHHHHHHHH
NLGFAIVDRLLGGAGNSLDKERDFSEIELSILERIFTVCVNLMQEPWENVVKVNPRLERI
CCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCHHHHH
ETNSQFAQIISPSETIAIVTINLKIGDVEGLVNICLPFATLEPVMDKLNTKYWFSNMQEK
HCCHHHHHHCCCCCEEEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCHHC
DTNTYEDAIETVIDKAMIPVKAVLGTSKISVSDFANLQIGDIIRIDKKVDQELEVFVGNI
CCCHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHCCCCCCCEEEHHHHHHHHHHHHHHHH
KKFKALPGYYEDKYAVRVTEVIRKESE
HHHHCCCCCCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure 
GEVLSQSEIDNLLKALSSGELDVDEMKNTEEKQVKNYDFARPSKFSKEHLRTLEIIFEH
CCCCCHHHHHHHHHHHHCCCCCHHHHCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
FGRLLATNLPAYLRKSVSVDVVNSEVVIYSEFSNALSNPVLLGVVGMQPLSGNIIMEMAS
HHHHHHHCCHHHHHHCCEEEEECCCEEEEECHHHHHCCCEEEEEECCCCCCCHHHHHHHH
NLGFAIVDRLLGGAGNSLDKERDFSEIELSILERIFTVCVNLMQEPWENVVKVNPRLERI
CCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCHHHHH
ETNSQFAQIISPSETIAIVTINLKIGDVEGLVNICLPFATLEPVMDKLNTKYWFSNMQEK
HCCHHHHHHCCCCCEEEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCHHC
DTNTYEDAIETVIDKAMIPVKAVLGTSKISVSDFANLQIGDIIRIDKKVDQELEVFVGNI
CCCHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHCCCCCCCEEEHHHHHHHHHHHHHHHH
KKFKALPGYYEDKYAVRVTEVIRKESE
HHHHCCCCCCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8755894; 9665876; 8529894 [H]