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The map label for this gene is pilT [H]

Identifier: 238916723

GI number: 238916723

Start: 796290

End: 797354

Strand: Direct

Name: pilT [H]

Synonym: EUBELI_00785

Alternate gene names: 238916723

Gene position: 796290-797354 (Clockwise)

Preceding gene: 238916722

Following gene: 238916724

Centisome position: 37.14

GC content: 41.13

Gene sequence:

>1065_bases
ATGTTTACAGTAGAGCAGCTGCTTACGATAGCAAAAGAAAAAAAGGCAAGTGATGTACATGTTACAGTCGGACTGCCACC
AAGAGTCAGAATTAATGGAGACCTTGTTGATCTTGAATATCCAAGGCTTACTCCGGCAGATTGTGAAAAGCTTATTCTTG
ATATTATGGATGAGAGACAGGAAAATATGTTTAAGGACAGAGGAGAACTGGATTTCTCATTCTCGATTCCGAATATAGGC
AGATACAGAGTTAATGTGTTCAGGCAGAGAGGTTCTGTTGCGTGTGCAATGAGAATTGTTGGAACAGAAATCCCTTCCCC
TGAAAGTCTTGGTGTACCTAAGTCAGTTATTGACCTGCATTCCAAGAAGAGAGGGCTTGTGCTTGTAACAGGTCCTACGG
GTTCAGGTAAGTCTACAACACTTGCTTCACTGATTGACAGGATTAATGATAACCGCAATGCACATGTAATAACACTGGAG
GATCCTATAGAGTATCTTCACAGTCATAGAAAAGCCATGATTAACCAAAGGGAAATCGGGCTTGATACACGTTCTTATGC
AGATGCATTAAGGGCAGCGCTCCGAGAAGATCCTGATGTTATTCTTGTTGGAGAAATGAGAGATCTTGAGACAATATCAA
CAGCCATAACTGCGGCTGAGACAGGACACCTTGTATTTTCAACACTTCATACAATTGGCGCAGCGGCAACTATTGACAGA
ATTATTGATGTATTTCCGCCACACCAGCAGCAGCAGATAAGAATCCAGCTTGCAGTTGTTCTTGAAGCTGTTATTTCACA
GCAGCTTATACCGACGGCTGACAGAAGAGGAAGGGTAGCTGCATTTGAAGTAATGCATGGAACCATTCCTATAAAGAATC
TTATCCGTGAGGCTAAGACATATCAGATTACAAGTGTTTTACAGACAGGAAGAAAAGACGGAATGATATCTATGGATGAT
GCACTTCTTGATTTGTATAATAAGGGTGTGATTGATGCTGAGAATGCGGTAAGTTATGCTCAGGATGTTAATTATGTTAC
CAAGAGAGTTAATCAGTTCTTCTAA

Upstream 100 bases:

>100_bases
TTTGACATCCGGATTATCAGCCGGCAGGTCAGATGATAATGAAATTGTTGAAATAGAATTAGAGCAGATTGACTAATGGC
TGCGTGATGGGGGTTATATT

Downstream 100 bases:

>100_bases
TATGCGGATGTGATATGTAAGTAGGGGCGAGAAATGTTTGCAAAATTAAAAGAATGTAAAAGCATTAAAGAAATATATGA
GGTTCTCGGAAGAGAGCCTT

Product: twitching motility protein PilT

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 354; Mature: 354

Protein sequence:

>354_residues
MFTVEQLLTIAKEKKASDVHVTVGLPPRVRINGDLVDLEYPRLTPADCEKLILDIMDERQENMFKDRGELDFSFSIPNIG
RYRVNVFRQRGSVACAMRIVGTEIPSPESLGVPKSVIDLHSKKRGLVLVTGPTGSGKSTTLASLIDRINDNRNAHVITLE
DPIEYLHSHRKAMINQREIGLDTRSYADALRAALREDPDVILVGEMRDLETISTAITAAETGHLVFSTLHTIGAAATIDR
IIDVFPPHQQQQIRIQLAVVLEAVISQQLIPTADRRGRVAAFEVMHGTIPIKNLIREAKTYQITSVLQTGRKDGMISMDD
ALLDLYNKGVIDAENAVSYAQDVNYVTKRVNQFF

Sequences:

>Translated_354_residues
MFTVEQLLTIAKEKKASDVHVTVGLPPRVRINGDLVDLEYPRLTPADCEKLILDIMDERQENMFKDRGELDFSFSIPNIG
RYRVNVFRQRGSVACAMRIVGTEIPSPESLGVPKSVIDLHSKKRGLVLVTGPTGSGKSTTLASLIDRINDNRNAHVITLE
DPIEYLHSHRKAMINQREIGLDTRSYADALRAALREDPDVILVGEMRDLETISTAITAAETGHLVFSTLHTIGAAATIDR
IIDVFPPHQQQQIRIQLAVVLEAVISQQLIPTADRRGRVAAFEVMHGTIPIKNLIREAKTYQITSVLQTGRKDGMISMDD
ALLDLYNKGVIDAENAVSYAQDVNYVTKRVNQFF
>Mature_354_residues
MFTVEQLLTIAKEKKASDVHVTVGLPPRVRINGDLVDLEYPRLTPADCEKLILDIMDERQENMFKDRGELDFSFSIPNIG
RYRVNVFRQRGSVACAMRIVGTEIPSPESLGVPKSVIDLHSKKRGLVLVTGPTGSGKSTTLASLIDRINDNRNAHVITLE
DPIEYLHSHRKAMINQREIGLDTRSYADALRAALREDPDVILVGEMRDLETISTAITAAETGHLVFSTLHTIGAAATIDR
IIDVFPPHQQQQIRIQLAVVLEAVISQQLIPTADRRGRVAAFEVMHGTIPIKNLIREAKTYQITSVLQTGRKDGMISMDD
ALLDLYNKGVIDAENAVSYAQDVNYVTKRVNQFF

Specific function: May be involved in pilus retraction [H]

COG id: COG2805

COG function: function code NU; Tfp pilus assembly protein, pilus retraction ATPase PilT

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI87082188, Length=320, Percent_Identity=44.6875, Blast_Score=281, Evalue=5e-77,
Organism=Escherichia coli, GI1786296, Length=280, Percent_Identity=35, Blast_Score=111, Evalue=9e-26,
Organism=Escherichia coli, GI1789723, Length=122, Percent_Identity=50, Blast_Score=109, Evalue=3e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR006321
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 39422; Mature: 39422

Theoretical pI: Translated: 6.61; Mature: 6.61

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFTVEQLLTIAKEKKASDVHVTVGLPPRVRINGDLVDLEYPRLTPADCEKLILDIMDERQ
CCCHHHHHHHHHHCCCCCEEEEECCCCCEEECCCEEECCCCCCCHHHHHHHHHHHHHHHH
ENMFKDRGELDFSFSIPNIGRYRVNVFRQRGSVACAMRIVGTEIPSPESLGVPKSVIDLH
HHHHHCCCCEEEEECCCCCCHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCHHHHHHH
SKKRGLVLVTGPTGSGKSTTLASLIDRINDNRNAHVITLEDPIEYLHSHRKAMINQREIG
CCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHHHHHHHCC
LDTRSYADALRAALREDPDVILVGEMRDLETISTAITAAETGHLVFSTLHTIGAAATIDR
CCHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
IIDVFPPHQQQQIRIQLAVVLEAVISQQLIPTADRRGRVAAFEVMHGTIPIKNLIREAKT
HHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEHHHCCCCHHHHHHHHHH
YQITSVLQTGRKDGMISMDDALLDLYNKGVIDAENAVSYAQDVNYVTKRVNQFF
HHHHHHHHCCCCCCCEEHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MFTVEQLLTIAKEKKASDVHVTVGLPPRVRINGDLVDLEYPRLTPADCEKLILDIMDERQ
CCCHHHHHHHHHHCCCCCEEEEECCCCCEEECCCEEECCCCCCCHHHHHHHHHHHHHHHH
ENMFKDRGELDFSFSIPNIGRYRVNVFRQRGSVACAMRIVGTEIPSPESLGVPKSVIDLH
HHHHHCCCCEEEEECCCCCCHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCHHHHHHH
SKKRGLVLVTGPTGSGKSTTLASLIDRINDNRNAHVITLEDPIEYLHSHRKAMINQREIG
CCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHHHHHHHCC
LDTRSYADALRAALREDPDVILVGEMRDLETISTAITAAETGHLVFSTLHTIGAAATIDR
CCHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
IIDVFPPHQQQQIRIQLAVVLEAVISQQLIPTADRRGRVAAFEVMHGTIPIKNLIREAKT
HHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEHHHCCCCHHHHHHHHHH
YQITSVLQTGRKDGMISMDDALLDLYNKGVIDAENAVSYAQDVNYVTKRVNQFF
HHHHHHHHCCCCCCCEEHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1676385; 10984043 [H]