Click here to switch to the map view.

The map label for this gene is epsE [H]

Identifier: 238916722

GI number: 238916722

Start: 794418

End: 796265

Strand: Direct

Name: epsE [H]

Synonym: EUBELI_00784

Alternate gene names: 238916722

Gene position: 794418-796265 (Clockwise)

Preceding gene: 238916721

Following gene: 238916723

Centisome position: 37.05

GC content: 39.5

Gene sequence:

>1848_bases
GTGAATTATAGAAAGAAAATACGTTTGGGAGACGTACTTGTAAAAAAAGGCATAATTGATGAGAATCAGTTACAGACTGC
ACTTTCAAGACAGAGAGAGCAGGGCAAGATGCTTGGTGAAATGGTTATTGCACTTGGATATGCAACACAGAGGGATATTA
ATGAAGCACTTTGCGACAGTCTTGGAATAGATTTTGTTGATATGAGAGAAACAGATGTCAGCGAAGATGTACTTTCTATG
CTTGATGAAAATATTATGAGAAAGTATACGCTTGTTCCTCTTGGGGATGCACCTGATAATCCGGGAGCAATAAGAGTTGC
TATGGCTGATCCTACTAATATTCTTGCCATGGATGATATTAATATTGTTACAGGCAAGCAGGTAGTTCCTGTTCTTGCTA
ATGCGTCTGATATTAATGCTTTCTTTGATAAAGCATTTGGACAGAAGCAGGCACAGAGTATTGTTGACCTTTATAAGAAG
GAACAGGGGGACGTATTCAAGGAAGAGACTAAGGAAGATAAGGCAAGAAGAGAAGAAATTGAGAATGCGCCTATTGTTCA
GCTTATTAACAGTGTTATAGAGCAGGCGGTAAGACAGAGAGCTTCCGATATACATATAGAGCCAATGGAGAAGAGTATCC
GTGTAAGGTACAGAATTGATGGTAATCTCCGTGAAATTATTGATTATGATAATACGCTTCTTGGTGCTATCACAACACGT
ATAAAGATTATGTCAGGTATGGATATCTCTGAAAAGAGAAAGCCACAGGATGGTCGTATTACTCTTAATGTAGACGGAAG
AGAGTATGATATCCGTGTATCTAATCTTCCTACTGTATATGGCGAGAAATGTGTTATGAGAATTGCGTCCAAAGAAGGGT
TTAACATTGATAAATCCAAGCTTGGACTTACACCGGCTGACCTTAAGGTATTTGATGATATCTTAAAGAACCCACATGGA
ATTATTCTTGTAACAGGACCTACAGGATCTGGTAAGTCTACGACACTTTACACAGCTTTAAGTGAACTGAATACAGAAGA
TGTTAATATTATCACAGTTGAAGATCCTGTCGAGGCTAATATTGATGGTATCAATCAGGTACAGGTTAATAATAAGGCTA
ACCTTACATTTGCAACTGCCTTAAGATCAATCCTTAGACAGGATCCGGATATCATTATGATTGGTGAGATCCGAGATGGT
GAAACAGCTGAGATTGCAGTAAGAGCTTCTATTACCGGACATCTGGTTGTTAGTACATTACATACTAACAGTACAGCAAG
TTCTGTTGCCCGTCTTGAAGATATGGGAATAGAATCTTACCTTATAGCTGATTCGCTTGTTGGTATTATTGCCCAGCGAC
TTGTAAGAAAGCTGTGTGACTGCAAGATGCCTAAAGAGGCAAGTGCAGCAGAGAAAGAAATGTTAGGCCTTAATCCGGAT
GAACCATTTACAGTATATGAACCATGTGGCTGTAAGCTTTGTAATGGAACAGGTTACTATGGACGACTTGGTATCTATGA
GATAATGAAGATAACACCTTCTATTAAGAGACTTATTTCTAAACATGCTGATGCAGAGGATATTAAGAATCAGGCAATCA
GTGAGGGAATGAATACCCTTAAGATGGCGGCAACCAATGCTGTTAAGGAAGGCGTCACAACAATTGCTGAGATGGTTAAG
GCTACTTATGAGGCAGAGGAAGATGATTCACAGCCTAATGCAAAGAATGCCAATGCCGGAGCAGTTAAGAAGCCGGCAGC
ACCAGCATCATCTTTGACATCCGGATTATCAGCCGGCAGGTCAGATGATAATGAAATTGTTGAAATAGAATTAGAGCAGA
TTGACTAA

Upstream 100 bases:

>100_bases
ATAAAATAAAAATACTATTTTGTAAATATCTGTTTATGTGATATAATTTAGTAAAATTGGTCAAAGCAGGGGGAGTTGAC
CTGCGGAAAGAGGTTGAAGG

Downstream 100 bases:

>100_bases
TGGCTGCGTGATGGGGGTTATATTATGTTTACAGTAGAGCAGCTGCTTACGATAGCAAAAGAAAAAAAGGCAAGTGATGT
ACATGTTACAGTCGGACTGC

Product: type IV pilus assembly protein PilB

Products: NA

Alternate protein names: Cholera toxin secretion protein epsE; Type II traffic warden ATPase [H]

Number of amino acids: Translated: 615; Mature: 615

Protein sequence:

>615_residues
MNYRKKIRLGDVLVKKGIIDENQLQTALSRQREQGKMLGEMVIALGYATQRDINEALCDSLGIDFVDMRETDVSEDVLSM
LDENIMRKYTLVPLGDAPDNPGAIRVAMADPTNILAMDDINIVTGKQVVPVLANASDINAFFDKAFGQKQAQSIVDLYKK
EQGDVFKEETKEDKARREEIENAPIVQLINSVIEQAVRQRASDIHIEPMEKSIRVRYRIDGNLREIIDYDNTLLGAITTR
IKIMSGMDISEKRKPQDGRITLNVDGREYDIRVSNLPTVYGEKCVMRIASKEGFNIDKSKLGLTPADLKVFDDILKNPHG
IILVTGPTGSGKSTTLYTALSELNTEDVNIITVEDPVEANIDGINQVQVNNKANLTFATALRSILRQDPDIIMIGEIRDG
ETAEIAVRASITGHLVVSTLHTNSTASSVARLEDMGIESYLIADSLVGIIAQRLVRKLCDCKMPKEASAAEKEMLGLNPD
EPFTVYEPCGCKLCNGTGYYGRLGIYEIMKITPSIKRLISKHADAEDIKNQAISEGMNTLKMAATNAVKEGVTTIAEMVK
ATYEAEEDDSQPNAKNANAGAVKKPAAPASSLTSGLSAGRSDDNEIVEIELEQID

Sequences:

>Translated_615_residues
MNYRKKIRLGDVLVKKGIIDENQLQTALSRQREQGKMLGEMVIALGYATQRDINEALCDSLGIDFVDMRETDVSEDVLSM
LDENIMRKYTLVPLGDAPDNPGAIRVAMADPTNILAMDDINIVTGKQVVPVLANASDINAFFDKAFGQKQAQSIVDLYKK
EQGDVFKEETKEDKARREEIENAPIVQLINSVIEQAVRQRASDIHIEPMEKSIRVRYRIDGNLREIIDYDNTLLGAITTR
IKIMSGMDISEKRKPQDGRITLNVDGREYDIRVSNLPTVYGEKCVMRIASKEGFNIDKSKLGLTPADLKVFDDILKNPHG
IILVTGPTGSGKSTTLYTALSELNTEDVNIITVEDPVEANIDGINQVQVNNKANLTFATALRSILRQDPDIIMIGEIRDG
ETAEIAVRASITGHLVVSTLHTNSTASSVARLEDMGIESYLIADSLVGIIAQRLVRKLCDCKMPKEASAAEKEMLGLNPD
EPFTVYEPCGCKLCNGTGYYGRLGIYEIMKITPSIKRLISKHADAEDIKNQAISEGMNTLKMAATNAVKEGVTTIAEMVK
ATYEAEEDDSQPNAKNANAGAVKKPAAPASSLTSGLSAGRSDDNEIVEIELEQID
>Mature_615_residues
MNYRKKIRLGDVLVKKGIIDENQLQTALSRQREQGKMLGEMVIALGYATQRDINEALCDSLGIDFVDMRETDVSEDVLSM
LDENIMRKYTLVPLGDAPDNPGAIRVAMADPTNILAMDDINIVTGKQVVPVLANASDINAFFDKAFGQKQAQSIVDLYKK
EQGDVFKEETKEDKARREEIENAPIVQLINSVIEQAVRQRASDIHIEPMEKSIRVRYRIDGNLREIIDYDNTLLGAITTR
IKIMSGMDISEKRKPQDGRITLNVDGREYDIRVSNLPTVYGEKCVMRIASKEGFNIDKSKLGLTPADLKVFDDILKNPHG
IILVTGPTGSGKSTTLYTALSELNTEDVNIITVEDPVEANIDGINQVQVNNKANLTFATALRSILRQDPDIIMIGEIRDG
ETAEIAVRASITGHLVVSTLHTNSTASSVARLEDMGIESYLIADSLVGIIAQRLVRKLCDCKMPKEASAAEKEMLGLNPD
EPFTVYEPCGCKLCNGTGYYGRLGIYEIMKITPSIKRLISKHADAEDIKNQAISEGMNTLKMAATNAVKEGVTTIAEMVK
ATYEAEEDDSQPNAKNANAGAVKKPAAPASSLTSGLSAGRSDDNEIVEIELEQID

Specific function: Required for secretion of cholera toxin through the outer membrane [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=438, Percent_Identity=45.2054794520548, Blast_Score=387, Evalue=1e-108,
Organism=Escherichia coli, GI1786296, Length=411, Percent_Identity=42.8223844282238, Blast_Score=354, Evalue=7e-99,
Organism=Escherichia coli, GI87082188, Length=129, Percent_Identity=48.8372093023256, Blast_Score=117, Evalue=3e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013369
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 67527; Mature: 67527

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNYRKKIRLGDVLVKKGIIDENQLQTALSRQREQGKMLGEMVIALGYATQRDINEALCDS
CCCHHHCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
LGIDFVDMRETDVSEDVLSMLDENIMRKYTLVPLGDAPDNPGAIRVAMADPTNILAMDDI
HCCCEEECCCCCCHHHHHHHHHHHHHHHEEEEECCCCCCCCCEEEEEEECCCCEEEECCC
NIVTGKQVVPVLANASDINAFFDKAFGQKQAQSIVDLYKKEQGDVFKEETKEDKARREEI
EEECCCCHHHHHCCCHHHHHHHHHHHCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
ENAPIVQLINSVIEQAVRQRASDIHIEPMEKSIRVRYRIDGNLREIIDYDNTLLGAITTR
CCCHHHHHHHHHHHHHHHHHHHCCEEEECCCCEEEEEEECCCHHHHHCCCCHHHHHHHHH
IKIMSGMDISEKRKPQDGRITLNVDGREYDIRVSNLPTVYGEKCVMRIASKEGFNIDKSK
HHHCCCCCCHHHCCCCCCEEEEEECCCEEEEEECCCCCHHHHHHHHHHHCCCCCCCCHHH
LGLTPADLKVFDDILKNPHGIILVTGPTGSGKSTTLYTALSELNTEDVNIITVEDPVEAN
CCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHCCCCCEEEEEECCCCCCC
IDGINQVQVNNKANLTFATALRSILRQDPDIIMIGEIRDGETAEIAVRASITGHLVVSTL
CCCCEEEEECCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCEEEEEEEECCEEEEEEEE
HTNSTASSVARLEDMGIESYLIADSLVGIIAQRLVRKLCDCKMPKEASAAEKEMLGLNPD
ECCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCC
EPFTVYEPCGCKLCNGTGYYGRLGIYEIMKITPSIKRLISKHADAEDIKNQAISEGMNTL
CCEEEECCCCCEEECCCCCCCCHHHHHHHHHCHHHHHHHHHCCCHHHHHHHHHHHHHHHH
KMAATNAVKEGVTTIAEMVKATYEAEEDDSQPNAKNANAGAVKKPAAPASSLTSGLSAGR
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCC
SDDNEIVEIELEQID
CCCCCEEEEEEEECC
>Mature Secondary Structure
MNYRKKIRLGDVLVKKGIIDENQLQTALSRQREQGKMLGEMVIALGYATQRDINEALCDS
CCCHHHCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
LGIDFVDMRETDVSEDVLSMLDENIMRKYTLVPLGDAPDNPGAIRVAMADPTNILAMDDI
HCCCEEECCCCCCHHHHHHHHHHHHHHHEEEEECCCCCCCCCEEEEEEECCCCEEEECCC
NIVTGKQVVPVLANASDINAFFDKAFGQKQAQSIVDLYKKEQGDVFKEETKEDKARREEI
EEECCCCHHHHHCCCHHHHHHHHHHHCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
ENAPIVQLINSVIEQAVRQRASDIHIEPMEKSIRVRYRIDGNLREIIDYDNTLLGAITTR
CCCHHHHHHHHHHHHHHHHHHHCCEEEECCCCEEEEEEECCCHHHHHCCCCHHHHHHHHH
IKIMSGMDISEKRKPQDGRITLNVDGREYDIRVSNLPTVYGEKCVMRIASKEGFNIDKSK
HHHCCCCCCHHHCCCCCCEEEEEECCCEEEEEECCCCCHHHHHHHHHHHCCCCCCCCHHH
LGLTPADLKVFDDILKNPHGIILVTGPTGSGKSTTLYTALSELNTEDVNIITVEDPVEAN
CCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHCCCCCEEEEEECCCCCCC
IDGINQVQVNNKANLTFATALRSILRQDPDIIMIGEIRDGETAEIAVRASITGHLVVSTL
CCCCEEEEECCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCEEEEEEEECCEEEEEEEE
HTNSTASSVARLEDMGIESYLIADSLVGIIAQRLVRKLCDCKMPKEASAAEKEMLGLNPD
ECCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCC
EPFTVYEPCGCKLCNGTGYYGRLGIYEIMKITPSIKRLISKHADAEDIKNQAISEGMNTL
CCEEEECCCCCEEECCCCCCCCHHHHHHHHHCHHHHHHHHHCCCHHHHHHHHHHHHHHHH
KMAATNAVKEGVTTIAEMVKATYEAEEDDSQPNAKNANAGAVKKPAAPASSLTSGLSAGR
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCC
SDDNEIVEIELEQID
CCCCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8423007; 10952301 [H]