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The map label for this gene is rnjA [H]

Identifier: 238916714

GI number: 238916714

Start: 786158

End: 787828

Strand: Direct

Name: rnjA [H]

Synonym: EUBELI_00776

Alternate gene names: 238916714

Gene position: 786158-787828 (Clockwise)

Preceding gene: 238916713

Following gene: 238916715

Centisome position: 36.66

GC content: 37.4

Gene sequence:

>1671_bases
ATGAAAAAAGCAGAGAATGCGTCTGTCAGGATTATACCATTGGGTGGTCTTGAGCAGATAGGAATGAACATTACTGCCAT
TGAGTATGATGACAGTATTATTGTTGTGGATTGTGGCTTATCTTTCCCAGAGGAAGATATGCTTGGTATTGATTTAGTTA
TACCGGATGTTACTTATCTTAAGGAGAATCTTAATAAGGTTAAAGGATTCGTTATTACTCATGGACATGAGGATCATATT
GGTGCAATTCCATATGTTTTAAAGGATGTCAATGTTCCTATATACGCTACTAAGCTTACTATGGGACTTATAGAGTCAAA
GCTTAAGGAACATAATATGTTAAAGTCAGTTAAGAGAAAGGTTGTTAAGTATGGACAATCTGTAACTCTTGGCGATTTCA
GAGTTGAGTTCATAAGAACTAACCATAGTATTGCAGATGCCGCAGCACTTGCTATATACTCACCGGCAGGAATTATTGTC
CACACAGGAGATTTTAAGGTTGATTATACACCTGTATATGGTGATCCTATTGATCTTCAAAGACTTGGTGAACTTGGAAA
GAAGGGTGTACTTGCACTTATGTGCGACAGTACGAATGCTTTAAGACCTGGTTTTACTATGTCAGAGAAGACAGTTGGTG
CTACATTTGAAAAGATTTTTAATGATAATAAGAACAGCAGAATTATTATTGCTACATTTGCATCTAATGTAGACAGAGTA
CAGCAGATTATTAATTCAGCGGTTAAATATGGTAGAAAAGTCTGCGTTGAAGGCCGAAGCATGGTTAATATTATTGAGGT
TGCAGAAGACCTTGATTATCTTAAGATTCCGGACGGAACTCTTATTGAAACGGATGAGATGAAGAATTATACACCTGAGC
AGATTGTTCTTATTACAACAGGTAGTCAGGGAGAGTCAATGGCTGCTCTTTCAAGAATGGCAGCTAATCTTCATAGAAAG
GTATCTATCCAGCTTGGCGACTGTGTAGTATTTTCATCAACTCCTATACCTGGTAATGAGAAGTCAGTAGCCAAGGTTAT
TAATGAGCTTGGTATGAAGGGGGCTAAGGTTATATTTCAGGATACACATGTATCAGGACATGCATGTCAGGAAGAGATTA
AGCTTATATATTCTCTTGTAAAGCCTAAGTATTCTATTCCTGTTCACGGTGAGTACAGACACCTTGTTGCACAGAGAGAT
GTTGTTATGTCACTTGGATATGACAAGGATCATGTTATTATTGCCAAATCAGGAGATGTGTTAGAACTTAATGATGAGCA
TGCACAGGTTGTAGATCATGTCCAGACAGGAGCTATTTTTGTTGATGGACTTGGCGTAGGTGATGTAGGAAATATTGTAT
TAAGAGACAGACAGAACCTTGCAGAGAATGGTATTATCATTGTTGTTCTTACACTTGAAAAATATACCGGACAGCTTATA
GCAGGCCCGGATATTGTTACAAGAGGTTTTGTGTATGTAAGAGAGGCTGAAGAGCTTCTTGATGAGGCAAGAGCAATTGT
ATCTGATTCAGTTGAAAAGTGTCTTGATAAGAATATTACTGACTGGAGTAAGATTAAGAATATTATTAAAGATGACTTGA
GCGAGTATCTCTGGAAGAAGATTAAGAGAAATCCTGTGATACTTCCAATCATTATGGAGGCACAGATATAA

Upstream 100 bases:

>100_bases
GTAGGCAAGGTGTTTTCTGAATTAATGGATGATGATGTTGAACTTAAATAAGGTGTGAGCCTTATTCATATTTTATATTT
TATTTTATTATGAGGTGTTT

Downstream 100 bases:

>100_bases
TATATGTTGCCGGATATTACTGCAGACAGTTGGAGAGGTGTTTTTCATGCGTAGAATTGATGAACTTAAGAAAGAGATTA
TACATGAAATTCTTAACAGT

Product: hypothetical protein

Products: NA

Alternate protein names: RNase J1 [H]

Number of amino acids: Translated: 556; Mature: 556

Protein sequence:

>556_residues
MKKAENASVRIIPLGGLEQIGMNITAIEYDDSIIVVDCGLSFPEEDMLGIDLVIPDVTYLKENLNKVKGFVITHGHEDHI
GAIPYVLKDVNVPIYATKLTMGLIESKLKEHNMLKSVKRKVVKYGQSVTLGDFRVEFIRTNHSIADAAALAIYSPAGIIV
HTGDFKVDYTPVYGDPIDLQRLGELGKKGVLALMCDSTNALRPGFTMSEKTVGATFEKIFNDNKNSRIIIATFASNVDRV
QQIINSAVKYGRKVCVEGRSMVNIIEVAEDLDYLKIPDGTLIETDEMKNYTPEQIVLITTGSQGESMAALSRMAANLHRK
VSIQLGDCVVFSSTPIPGNEKSVAKVINELGMKGAKVIFQDTHVSGHACQEEIKLIYSLVKPKYSIPVHGEYRHLVAQRD
VVMSLGYDKDHVIIAKSGDVLELNDEHAQVVDHVQTGAIFVDGLGVGDVGNIVLRDRQNLAENGIIIVVLTLEKYTGQLI
AGPDIVTRGFVYVREAEELLDEARAIVSDSVEKCLDKNITDWSKIKNIIKDDLSEYLWKKIKRNPVILPIIMEAQI

Sequences:

>Translated_556_residues
MKKAENASVRIIPLGGLEQIGMNITAIEYDDSIIVVDCGLSFPEEDMLGIDLVIPDVTYLKENLNKVKGFVITHGHEDHI
GAIPYVLKDVNVPIYATKLTMGLIESKLKEHNMLKSVKRKVVKYGQSVTLGDFRVEFIRTNHSIADAAALAIYSPAGIIV
HTGDFKVDYTPVYGDPIDLQRLGELGKKGVLALMCDSTNALRPGFTMSEKTVGATFEKIFNDNKNSRIIIATFASNVDRV
QQIINSAVKYGRKVCVEGRSMVNIIEVAEDLDYLKIPDGTLIETDEMKNYTPEQIVLITTGSQGESMAALSRMAANLHRK
VSIQLGDCVVFSSTPIPGNEKSVAKVINELGMKGAKVIFQDTHVSGHACQEEIKLIYSLVKPKYSIPVHGEYRHLVAQRD
VVMSLGYDKDHVIIAKSGDVLELNDEHAQVVDHVQTGAIFVDGLGVGDVGNIVLRDRQNLAENGIIIVVLTLEKYTGQLI
AGPDIVTRGFVYVREAEELLDEARAIVSDSVEKCLDKNITDWSKIKNIIKDDLSEYLWKKIKRNPVILPIIMEAQI
>Mature_556_residues
MKKAENASVRIIPLGGLEQIGMNITAIEYDDSIIVVDCGLSFPEEDMLGIDLVIPDVTYLKENLNKVKGFVITHGHEDHI
GAIPYVLKDVNVPIYATKLTMGLIESKLKEHNMLKSVKRKVVKYGQSVTLGDFRVEFIRTNHSIADAAALAIYSPAGIIV
HTGDFKVDYTPVYGDPIDLQRLGELGKKGVLALMCDSTNALRPGFTMSEKTVGATFEKIFNDNKNSRIIIATFASNVDRV
QQIINSAVKYGRKVCVEGRSMVNIIEVAEDLDYLKIPDGTLIETDEMKNYTPEQIVLITTGSQGESMAALSRMAANLHRK
VSIQLGDCVVFSSTPIPGNEKSVAKVINELGMKGAKVIFQDTHVSGHACQEEIKLIYSLVKPKYSIPVHGEYRHLVAQRD
VVMSLGYDKDHVIIAKSGDVLELNDEHAQVVDHVQTGAIFVDGLGVGDVGNIVLRDRQNLAENGIIIVVLTLEKYTGQLI
AGPDIVTRGFVYVREAEELLDEARAIVSDSVEKCLDKNITDWSKIKNIIKDDLSEYLWKKIKRNPVILPIIMEAQI

Specific function: Cleaves the 5'-leader sequence of certain mRNAs. May play a role in maturation and stability of specific mRNAs. Is also required for the maturation of the 16S rRNA. Acts preferentially on 16S rRNA precursors after association of the 30S and 50S ribosomal

COG id: COG0595

COG function: function code R; Predicted hydrolase of the metallo-beta-lactamase superfamily

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. RNA-metabolizing metallo-beta-lactamase-like family. RNase J subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001279
- InterPro:   IPR011108
- InterPro:   IPR004613
- InterPro:   IPR001587 [H]

Pfam domain/function: PF00753 Lactamase_B; PF07521 RMMBL [H]

EC number: NA

Molecular weight: Translated: 61399; Mature: 61399

Theoretical pI: Translated: 5.78; Mature: 5.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKAENASVRIIPLGGLEQIGMNITAIEYDDSIIVVDCGLSFPEEDMLGIDLVIPDVTYL
CCCCCCCCEEEEECCCHHHHCCEEEEEEECCCEEEEECCCCCCCCCCCEEEEECCCHHHH
KENLNKVKGFVITHGHEDHIGAIPYVLKDVNVPIYATKLTMGLIESKLKEHNMLKSVKRK
HHHHHHHCCEEEECCCCCCCCCCCHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHH
VVKYGQSVTLGDFRVEFIRTNHSIADAAALAIYSPAGIIVHTGDFKVDYTPVYGDPIDLQ
HHHCCCCEEECCEEEEEEECCCCHHHHHHEEEECCCEEEEECCCEEEEEEECCCCCCCHH
RLGELGKKGVLALMCDSTNALRPGFTMSEKTVGATFEKIFNDNKNSRIIIATFASNVDRV
HHHHCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEEHHCCHHHH
QQIINSAVKYGRKVCVEGRSMVNIIEVAEDLDYLKIPDGTLIETDEMKNYTPEQIVLITT
HHHHHHHHHHCHHEEECCCHHHHHHHHHHCCCEEECCCCCEEECHHCCCCCCCEEEEEEE
GSQGESMAALSRMAANLHRKVSIQLGDCVVFSSTPIPGNEKSVAKVINELGMKGAKVIFQ
CCCCCHHHHHHHHHHHHCEEEEEEECCEEEEECCCCCCCHHHHHHHHHHHCCCCCEEEEE
DTHVSGHACQEEIKLIYSLVKPKYSIPVHGEYRHLVAQRDVVMSLGYDKDHVIIAKSGDV
ECCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHEHEECCCCCCEEEEECCCCE
LELNDEHAQVVDHVQTGAIFVDGLGVGDVGNIVLRDRQNLAENGIIIVVLTLEKYTGQLI
EEECCHHHHHHHHHCCCEEEEECCCCCCCCCEEEECCHHHCCCCEEEEEEEECCCCCCEE
AGPDIVTRGFVYVREAEELLDEARAIVSDSVEKCLDKNITDWSKIKNIIKDDLSEYLWKK
ECCCHHHCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
IKRNPVILPIIMEAQI
HCCCCEEEEEEEECCC
>Mature Secondary Structure
MKKAENASVRIIPLGGLEQIGMNITAIEYDDSIIVVDCGLSFPEEDMLGIDLVIPDVTYL
CCCCCCCCEEEEECCCHHHHCCEEEEEEECCCEEEEECCCCCCCCCCCEEEEECCCHHHH
KENLNKVKGFVITHGHEDHIGAIPYVLKDVNVPIYATKLTMGLIESKLKEHNMLKSVKRK
HHHHHHHCCEEEECCCCCCCCCCCHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHH
VVKYGQSVTLGDFRVEFIRTNHSIADAAALAIYSPAGIIVHTGDFKVDYTPVYGDPIDLQ
HHHCCCCEEECCEEEEEEECCCCHHHHHHEEEECCCEEEEECCCEEEEEEECCCCCCCHH
RLGELGKKGVLALMCDSTNALRPGFTMSEKTVGATFEKIFNDNKNSRIIIATFASNVDRV
HHHHCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEEHHCCHHHH
QQIINSAVKYGRKVCVEGRSMVNIIEVAEDLDYLKIPDGTLIETDEMKNYTPEQIVLITT
HHHHHHHHHHCHHEEECCCHHHHHHHHHHCCCEEECCCCCEEECHHCCCCCCCEEEEEEE
GSQGESMAALSRMAANLHRKVSIQLGDCVVFSSTPIPGNEKSVAKVINELGMKGAKVIFQ
CCCCCHHHHHHHHHHHHCEEEEEEECCEEEEECCCCCCCHHHHHHHHHHHCCCCCEEEEE
DTHVSGHACQEEIKLIYSLVKPKYSIPVHGEYRHLVAQRDVVMSLGYDKDHVIIAKSGDV
ECCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHEHEECCCCCCEEEEECCCCE
LELNDEHAQVVDHVQTGAIFVDGLGVGDVGNIVLRDRQNLAENGIIIVVLTLEKYTGQLI
EEECCHHHHHHHHHCCCEEEEECCCCCCCCCEEEECCHHHCCCCEEEEEEEECCCCCCEE
AGPDIVTRGFVYVREAEELLDEARAIVSDSVEKCLDKNITDWSKIKNIIKDDLSEYLWKK
ECCCHHHCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
IKRNPVILPIIMEAQI
HCCCCEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969500; 9384377 [H]