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The map label for this gene is mscS [H]

Identifier: 238916707

GI number: 238916707

Start: 782502

End: 783389

Strand: Direct

Name: mscS [H]

Synonym: EUBELI_00769

Alternate gene names: 238916707

Gene position: 782502-783389 (Clockwise)

Preceding gene: 238916706

Following gene: 238916708

Centisome position: 36.49

GC content: 37.61

Gene sequence:

>888_bases
ATGGGGTTCATAGGACTTGTGGCAGATGCAGCCACAACAATCCAGCAGGAAACGGGTGAGATAACCAGATTCTTTTCTAT
TGCCCATGTTAAGCAGCTTCTCAATGATTTAGGAAACTGGTCGGTTTCACTTATTGGTAAAATTGTTATTGTTGTCTTAA
TATGGTTTATTGGCAAGAAGATAATCAGGGTACTTGATAAGCTTGTAAAGAAGATGCTGGACAGAAGCACGCTAGATAAA
GGCGTTGTTAATTTTGTGGTATCAGTGCTTAAGTTTGTAATGTATGCAATACTTATCATGATAGTTGTGGATAAGCTGGG
ATTTCAGACGACATCACTGCTCACACTTTTTGGTTCTGCAGCACTTGCAATTGGTATGTCCCTGCAGGGAAGCCTTTCGA
ACTTTGCAGGAGGAATTCTTATTCTTATATTCAAGCCTTTTAAGGTTGGCGATTATATTATAGTCGGAACTAATGAAGGA
ACTGTAAAGTCTATAGAGATTCTTTACACAAGGCTTGTTACTATTGATAATAAAGTAGTAATGCTTCCGAATGGTTCACT
TTCTAATTCAAGCATTGTTAATGTAGGCGCAGAGAATACAAGAAGAATTGATATCCAGATAGGAATAGGCTATTCATCAG
ATATTCCTAAAGCCAAGAAGCTTCTTGAACAGGTTATTAACAGCGAGAAGGGAATACTTAAGGACAAGGATATTCTGGTT
GTTGTCAAGAGTCTTGATGAAAGCTGTGTAACACTTGAAACAAGAGCGTGGGTTAATACAGCAGACTACTGGAATGTAAG
ATTTAACCTGCTTGAAAGATATAAGAATATATTTGATGAGAATGGAATTGAGATTCCGTTCAACCAGCTTGATGTACATA
TGAAGTAG

Upstream 100 bases:

>100_bases
TGAAGAAGCAGCCCGTGCAAGTGAAGAGATTGATATAGAAACTAAGAAGAAGTTAGAGCAGCTTAAAAAGAAGATGAAAT
AATACAAAGGGGGATCAGCT

Downstream 100 bases:

>100_bases
TTTTTTTTAGGATGCCGCGAATGTGGCATCCTTTTTTAGTGCCTTTAAATGTGTTCACAAAACTACACTGATTTTACTTA
TTTTTTACATAGTTTGGTCA

Product: MscS family small conductance mechanosensitive ion channel

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 295; Mature: 294

Protein sequence:

>295_residues
MGFIGLVADAATTIQQETGEITRFFSIAHVKQLLNDLGNWSVSLIGKIVIVVLIWFIGKKIIRVLDKLVKKMLDRSTLDK
GVVNFVVSVLKFVMYAILIMIVVDKLGFQTTSLLTLFGSAALAIGMSLQGSLSNFAGGILILIFKPFKVGDYIIVGTNEG
TVKSIEILYTRLVTIDNKVVMLPNGSLSNSSIVNVGAENTRRIDIQIGIGYSSDIPKAKKLLEQVINSEKGILKDKDILV
VVKSLDESCVTLETRAWVNTADYWNVRFNLLERYKNIFDENGIEIPFNQLDVHMK

Sequences:

>Translated_295_residues
MGFIGLVADAATTIQQETGEITRFFSIAHVKQLLNDLGNWSVSLIGKIVIVVLIWFIGKKIIRVLDKLVKKMLDRSTLDK
GVVNFVVSVLKFVMYAILIMIVVDKLGFQTTSLLTLFGSAALAIGMSLQGSLSNFAGGILILIFKPFKVGDYIIVGTNEG
TVKSIEILYTRLVTIDNKVVMLPNGSLSNSSIVNVGAENTRRIDIQIGIGYSSDIPKAKKLLEQVINSEKGILKDKDILV
VVKSLDESCVTLETRAWVNTADYWNVRFNLLERYKNIFDENGIEIPFNQLDVHMK
>Mature_294_residues
GFIGLVADAATTIQQETGEITRFFSIAHVKQLLNDLGNWSVSLIGKIVIVVLIWFIGKKIIRVLDKLVKKMLDRSTLDKG
VVNFVVSVLKFVMYAILIMIVVDKLGFQTTSLLTLFGSAALAIGMSLQGSLSNFAGGILILIFKPFKVGDYIIVGTNEGT
VKSIEILYTRLVTIDNKVVMLPNGSLSNSSIVNVGAENTRRIDIQIGIGYSSDIPKAKKLLEQVINSEKGILKDKDILVV
VKSLDESCVTLETRAWVNTADYWNVRFNLLERYKNIFDENGIEIPFNQLDVHMK

Specific function: Mechanosensitive channel that participates in the regulation of osmotic pressure changes within the cell. Forms an ion channel of 1.0 nanosiemens conductance with a slight preference for anions. The channel is sensitive to voltage [H]

COG id: COG0668

COG function: function code M; Small-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscS (TC 1.A.23) family [H]

Homologues:

Organism=Escherichia coli, GI1789291, Length=269, Percent_Identity=35.3159851301115, Blast_Score=187, Evalue=7e-49,
Organism=Escherichia coli, GI1786670, Length=260, Percent_Identity=27.6923076923077, Blast_Score=103, Evalue=9e-24,
Organism=Escherichia coli, GI1787591, Length=214, Percent_Identity=26.6355140186916, Blast_Score=81, Evalue=9e-17,
Organism=Escherichia coli, GI2367355, Length=151, Percent_Identity=23.841059602649, Blast_Score=65, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010920
- InterPro:   IPR011066
- InterPro:   IPR006685
- InterPro:   IPR006686
- InterPro:   IPR011014
- InterPro:   IPR008910 [H]

Pfam domain/function: PF00924 MS_channel; PF05552 TM_helix [H]

EC number: NA

Molecular weight: Translated: 32715; Mature: 32584

Theoretical pI: Translated: 9.48; Mature: 9.48

Prosite motif: PS01246 UPF0003

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGFIGLVADAATTIQQETGEITRFFSIAHVKQLLNDLGNWSVSLIGKIVIVVLIWFIGKK
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
IIRVLDKLVKKMLDRSTLDKGVVNFVVSVLKFVMYAILIMIVVDKLGFQTTSLLTLFGSA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
ALAIGMSLQGSLSNFAGGILILIFKPFKVGDYIIVGTNEGTVKSIEILYTRLVTIDNKVV
HHHHCCCCCCCHHHHCCCEEEEEEECCCCCCEEEEECCCCCHHHHHHHHHHHHEECCEEE
MLPNGSLSNSSIVNVGAENTRRIDIQIGIGYSSDIPKAKKLLEQVINSEKGILKDKDILV
EECCCCCCCCCEEEECCCCCEEEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCCCEE
VVKSLDESCVTLETRAWVNTADYWNVRFNLLERYKNIFDENGIEIPFNQLDVHMK
EEECCCCCCEEEEHHHEECCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCEECC
>Mature Secondary Structure 
GFIGLVADAATTIQQETGEITRFFSIAHVKQLLNDLGNWSVSLIGKIVIVVLIWFIGKK
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
IIRVLDKLVKKMLDRSTLDKGVVNFVVSVLKFVMYAILIMIVVDKLGFQTTSLLTLFGSA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
ALAIGMSLQGSLSNFAGGILILIFKPFKVGDYIIVGTNEGTVKSIEILYTRLVTIDNKVV
HHHHCCCCCCCHHHHCCCEEEEEEECCCCCCEEEEECCCCCHHHHHHHHHHHHEECCEEE
MLPNGSLSNSSIVNVGAENTRRIDIQIGIGYSSDIPKAKKLLEQVINSEKGILKDKDILV
EECCCCCCCCCEEEECCCCCEEEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCCCEE
VVKSLDESCVTLETRAWVNTADYWNVRFNLLERYKNIFDENGIEIPFNQLDVHMK
EEECCCCCCEEEEHHHEECCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12542086 [H]