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The map label for this gene is tgt

Identifier: 238916697

GI number: 238916697

Start: 769046

End: 770182

Strand: Direct

Name: tgt

Synonym: EUBELI_00759

Alternate gene names: 238916697

Gene position: 769046-770182 (Clockwise)

Preceding gene: 238916696

Following gene: 238916698

Centisome position: 35.87

GC content: 40.9

Gene sequence:

>1137_bases
ATGTATAAAATTTTAAAGACAGATGGCAGGGCAAAAAGAGCAGAGTTCACAACAGTACATGGAACTGTGCAGACACCTGT
ATTTATGAATGTCGGAACAGTTGCGGCCATTAAAGGCGCAGTTGCGACAACTGATTTACAGCAGATAGGAACGCAGATAG
AATTATCTAACACTTACCATCTTCATGTAAGACCGGGAGATAAGATTATTAAGCAGCTTGGAGGACTTCATAAGTTCATG
AACTGGGATAAACCGATTCTTACAGATTCAGGTGGATTTCAGGTGTTTTCACTTGCAGGACTCCGTAAGATTAAAGAAGA
AGGCGTTACTTTCCAGTCACATATTGACGGACATAAGATATTCATGGGACCAGAGGAAAGCATGCAGATACAGTCTAATC
TTGGTTCAACAATCGCAATGGCATTTGACGAATGTGCGCCGGCAAAGGCTGACAGAAAATATATCCAGAATTCTGTTGAC
AGAACATACCGCTGGTTAGAGCGTTGCAAGAAAGAGATGGCAAGACTGAACTCACTTCCTGATACGGTTAATCCTGATCA
GATGCTTTTCGGTATCGATCAGGGTGGTGTGTTCAATGATATAAGAATTGACCATGCTAAGAGAATATCAGAGTTAGACC
TTGACGGTTATGCAGTAGGCGGACTTGCCGTAGGTGAAACACATGAGGAAATGTATGATGTACTCGATAATGTAGTGCCA
TATCTTCCACAGGATAAGCCGACATACCTTATGGGTGTTGGAACACCAGCCAATATTCTTGAGTCAGTAGACAGAGGAAT
TGATTTCTTCGACTGCGTATATCCTTCAAGAAATGGCCGTCACGGACATGTATATACTAAGTTTGGAAAGATTAATCTTT
TTAATGCAAAGTATGAAACAGATACAGCACCAATTGAAGAAGGCTGTGGATGTCCATGCTGTCAGAATTATTCAAGAGCA
TATGTAAGGCATCTTCTTAAGGCTAAGGAAATGCTTGGTATGCGTCTGTGCGTTCTTCATAATCTTTACTACTACAACCA
TCTTATGACAGACATAAGAGCTGCGATTGAAGAAGGAAGATATGCAGGCTTCAAGGAAGAGGCATTATATCAGATGAAGA
CATATGATAAGCAGTAA

Upstream 100 bases:

>100_bases
AAGCTATGACAGCACTTACACTTGTTGACCTGATATTTGACAATATGACGGCTAGAATGGACAGAGTTAAAGAGTTTTAT
AGAAAATAAAAGGAGCAGAT

Downstream 100 bases:

>100_bases
TGCTGTATGTTTATAAATATTTTGTTAAAAATACATTTTGAGGTAGACAAGCAGCTCTCAATTATGTATTATATGTTAAG
TTTGAAAGAGACATTTTTAA

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase

Number of amino acids: Translated: 378; Mature: 378

Protein sequence:

>378_residues
MYKILKTDGRAKRAEFTTVHGTVQTPVFMNVGTVAAIKGAVATTDLQQIGTQIELSNTYHLHVRPGDKIIKQLGGLHKFM
NWDKPILTDSGGFQVFSLAGLRKIKEEGVTFQSHIDGHKIFMGPEESMQIQSNLGSTIAMAFDECAPAKADRKYIQNSVD
RTYRWLERCKKEMARLNSLPDTVNPDQMLFGIDQGGVFNDIRIDHAKRISELDLDGYAVGGLAVGETHEEMYDVLDNVVP
YLPQDKPTYLMGVGTPANILESVDRGIDFFDCVYPSRNGRHGHVYTKFGKINLFNAKYETDTAPIEEGCGCPCCQNYSRA
YVRHLLKAKEMLGMRLCVLHNLYYYNHLMTDIRAAIEEGRYAGFKEEALYQMKTYDKQ

Sequences:

>Translated_378_residues
MYKILKTDGRAKRAEFTTVHGTVQTPVFMNVGTVAAIKGAVATTDLQQIGTQIELSNTYHLHVRPGDKIIKQLGGLHKFM
NWDKPILTDSGGFQVFSLAGLRKIKEEGVTFQSHIDGHKIFMGPEESMQIQSNLGSTIAMAFDECAPAKADRKYIQNSVD
RTYRWLERCKKEMARLNSLPDTVNPDQMLFGIDQGGVFNDIRIDHAKRISELDLDGYAVGGLAVGETHEEMYDVLDNVVP
YLPQDKPTYLMGVGTPANILESVDRGIDFFDCVYPSRNGRHGHVYTKFGKINLFNAKYETDTAPIEEGCGCPCCQNYSRA
YVRHLLKAKEMLGMRLCVLHNLYYYNHLMTDIRAAIEEGRYAGFKEEALYQMKTYDKQ
>Mature_378_residues
MYKILKTDGRAKRAEFTTVHGTVQTPVFMNVGTVAAIKGAVATTDLQQIGTQIELSNTYHLHVRPGDKIIKQLGGLHKFM
NWDKPILTDSGGFQVFSLAGLRKIKEEGVTFQSHIDGHKIFMGPEESMQIQSNLGSTIAMAFDECAPAKADRKYIQNSVD
RTYRWLERCKKEMARLNSLPDTVNPDQMLFGIDQGGVFNDIRIDHAKRISELDLDGYAVGGLAVGETHEEMYDVLDNVVP
YLPQDKPTYLMGVGTPANILESVDRGIDFFDCVYPSRNGRHGHVYTKFGKINLFNAKYETDTAPIEEGCGCPCCQNYSRA
YVRHLLKAKEMLGMRLCVLHNLYYYNHLMTDIRAAIEEGRYAGFKEEALYQMKTYDKQ

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family

Homologues:

Organism=Homo sapiens, GI13654276, Length=363, Percent_Identity=39.9449035812672, Blast_Score=286, Evalue=3e-77,
Organism=Homo sapiens, GI13375872, Length=257, Percent_Identity=27.2373540856031, Blast_Score=107, Evalue=3e-23,
Organism=Escherichia coli, GI1786607, Length=363, Percent_Identity=48.4848484848485, Blast_Score=365, Evalue=1e-102,
Organism=Caenorhabditis elegans, GI17544680, Length=370, Percent_Identity=38.6486486486486, Blast_Score=257, Evalue=7e-69,
Organism=Caenorhabditis elegans, GI25146502, Length=240, Percent_Identity=26.25, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI19920510, Length=365, Percent_Identity=39.4520547945205, Blast_Score=273, Evalue=1e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TGT_EUBE2 (C4Z563)

Other databases:

- EMBL:   CP001104
- RefSeq:   YP_002930214.1
- GeneID:   7957307
- GenomeReviews:   CP001104_GR
- KEGG:   eel:EUBELI_00759
- OMA:   FMPVGTV
- ProtClustDB:   PRK00112
- HAMAP:   MF_00168
- InterPro:   IPR004803
- InterPro:   IPR002616
- Gene3D:   G3DSA:3.20.20.105
- PANTHER:   PTHR11962
- TIGRFAMs:   TIGR00430
- TIGRFAMs:   TIGR00449

Pfam domain/function: PF01702 TGT; SSF51713 tRNA_ribo_trans

EC number: =2.4.2.29

Molecular weight: Translated: 42663; Mature: 42663

Theoretical pI: Translated: 6.98; Mature: 6.98

Prosite motif: NA

Important sites: ACT_SITE 89-89 BINDING 90-90

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYKILKTDGRAKRAEFTTVHGTVQTPVFMNVGTVAAIKGAVATTDLQQIGTQIELSNTYH
CCEEECCCCCCCCCEEEEECCCCCCCEEEECCHHHHHHCHHHHHHHHHCCCEEEECCEEE
LHVRPGDKIIKQLGGLHKFMNWDKPILTDSGGFQVFSLAGLRKIKEEGVTFQSHIDGHKI
EEECCHHHHHHHHHHHHHHHCCCCCEEECCCCEEEEHHHHHHHHHHHCCEEECCCCCCEE
FMGPEESMQIQSNLGSTIAMAFDECAPAKADRKYIQNSVDRTYRWLERCKKEMARLNSLP
EECCCHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
DTVNPDQMLFGIDQGGVFNDIRIDHAKRISELDLDGYAVGGLAVGETHEEMYDVLDNVVP
CCCCCCCEEEECCCCCCCCCEEHHHHHHHHHCCCCCEEECCEEECCHHHHHHHHHHHHHC
YLPQDKPTYLMGVGTPANILESVDRGIDFFDCVYPSRNGRHGHVYTKFGKINLFNAKYET
CCCCCCCCEEEECCCHHHHHHHHHCCCCEEEHCCCCCCCCCCEEEEEECEEEEEECCCCC
DTAPIEEGCGCPCCQNYSRAYVRHLLKAKEMLGMRLCVLHNLYYYNHLMTDIRAAIEEGR
CCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
YAGFKEEALYQMKTYDKQ
CCCCHHHHHHHHHHCCCC
>Mature Secondary Structure
MYKILKTDGRAKRAEFTTVHGTVQTPVFMNVGTVAAIKGAVATTDLQQIGTQIELSNTYH
CCEEECCCCCCCCCEEEEECCCCCCCEEEECCHHHHHHCHHHHHHHHHCCCEEEECCEEE
LHVRPGDKIIKQLGGLHKFMNWDKPILTDSGGFQVFSLAGLRKIKEEGVTFQSHIDGHKI
EEECCHHHHHHHHHHHHHHHCCCCCEEECCCCEEEEHHHHHHHHHHHCCEEECCCCCCEE
FMGPEESMQIQSNLGSTIAMAFDECAPAKADRKYIQNSVDRTYRWLERCKKEMARLNSLP
EECCCHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
DTVNPDQMLFGIDQGGVFNDIRIDHAKRISELDLDGYAVGGLAVGETHEEMYDVLDNVVP
CCCCCCCEEEECCCCCCCCCEEHHHHHHHHHCCCCCEEECCEEECCHHHHHHHHHHHHHC
YLPQDKPTYLMGVGTPANILESVDRGIDFFDCVYPSRNGRHGHVYTKFGKINLFNAKYET
CCCCCCCCEEEECCCHHHHHHHHHCCCCEEEHCCCCCCCCCCEEEEEECEEEEEECCCCC
DTAPIEEGCGCPCCQNYSRAYVRHLLKAKEMLGMRLCVLHNLYYYNHLMTDIRAAIEEGR
CCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
YAGFKEEALYQMKTYDKQ
CCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA