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The map label for this gene is sigE [H]

Identifier: 238916673

GI number: 238916673

Start: 740474

End: 741226

Strand: Direct

Name: sigE [H]

Synonym: EUBELI_00735

Alternate gene names: 238916673

Gene position: 740474-741226 (Clockwise)

Preceding gene: 238916672

Following gene: 238916674

Centisome position: 34.53

GC content: 35.46

Gene sequence:

>753_bases
ATGGATAGAAATATGGTAGTAAAGGTTGCAATGGCGGATCATTTTCAGTTTAAGGTAATGCCTTCTATAAAGAATTTTTG
TTTATTAAAGCAGGGCGATGTACATTATATAGGTGGAACAGATATATTACCGGCACCACTTGGGGCAAGTGAGGAGCAGG
ATGCAATAGAAGCACTGGGGACAGCTTCTGATGCTGAAGCAAAGCACACTTTGATAGAACATAATTTAAGGCTTGTTGTA
TATATAGCAAAGAAATTTGATAATACAGGGGTTGGAGTAGAGGATTTAATATCAATAGGCACAATAGGGCTTATCAAGGC
AATTAATTCATATGACAAGGATAAAAATATCAAGCTTGCGACATATGCATCAAGGTGTATAGAAAATGAAATTCTTATGT
ATCTTAGAAGAAACAGTAAAATAAAGGCAGAAGTTTCAATAGACGAGCCATTAAATGTTGACTGGGATGGAAATGAGCTT
CTGTTGTCAGATATTATAGGAACGGATGAGGATATTATATATAGGGATATTGAAACAGAGGTTGAATGTAAAATGCTTAA
AAGAGCTATAAACCAGCTGCCGGAGCGTGAAAGAATAATAGTTGATTTAAGATTTGGTTTAAGCTCAGAGGATGGAGAAG
AAAAGACGCAGAAAGAGGTTGCAGATATGTTAGGAATTTCACAATCGTATATTTCAAGGCTCGAAAAGAAAATAATGCGT
CAGTTAAGAAAAGAAATGGTCAAAAGTTGTTGA

Upstream 100 bases:

>100_bases
CATGTCTCCCAGGCAGATTAATAAGAATGGGGATTATCATGCTCTTTTGGGGAAGGACATGATTATGTAGAGGAATTGGA
AGTTTGAAGAAAATAAAAGG

Downstream 100 bases:

>100_bases
CATTTGCCCCTTCCTTATGGACAATAGTACAAGAAATATTAATGGAGTACTATAATGAGAGGTCATAAGACATTTAGAAT
TGATAAAATATTTGGTCTGG

Product: sporulation sigma factor SigE

Products: NA

Alternate protein names: P31; Sigma-29; Stage II sporulation protein GB [H]

Number of amino acids: Translated: 250; Mature: 250

Protein sequence:

>250_residues
MDRNMVVKVAMADHFQFKVMPSIKNFCLLKQGDVHYIGGTDILPAPLGASEEQDAIEALGTASDAEAKHTLIEHNLRLVV
YIAKKFDNTGVGVEDLISIGTIGLIKAINSYDKDKNIKLATYASRCIENEILMYLRRNSKIKAEVSIDEPLNVDWDGNEL
LLSDIIGTDEDIIYRDIETEVECKMLKRAINQLPERERIIVDLRFGLSSEDGEEKTQKEVADMLGISQSYISRLEKKIMR
QLRKEMVKSC

Sequences:

>Translated_250_residues
MDRNMVVKVAMADHFQFKVMPSIKNFCLLKQGDVHYIGGTDILPAPLGASEEQDAIEALGTASDAEAKHTLIEHNLRLVV
YIAKKFDNTGVGVEDLISIGTIGLIKAINSYDKDKNIKLATYASRCIENEILMYLRRNSKIKAEVSIDEPLNVDWDGNEL
LLSDIIGTDEDIIYRDIETEVECKMLKRAINQLPERERIIVDLRFGLSSEDGEEKTQKEVADMLGISQSYISRLEKKIMR
QLRKEMVKSC
>Mature_250_residues
MDRNMVVKVAMADHFQFKVMPSIKNFCLLKQGDVHYIGGTDILPAPLGASEEQDAIEALGTASDAEAKHTLIEHNLRLVV
YIAKKFDNTGVGVEDLISIGTIGLIKAINSYDKDKNIKLATYASRCIENEILMYLRRNSKIKAEVSIDEPLNVDWDGNEL
LLSDIIGTDEDIIYRDIETEVECKMLKRAINQLPERERIIVDLRFGLSSEDGEEKTQKEVADMLGISQSYISRLEKKIMR
QLRKEMVKSC

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of sporulation specific genes [H]

COG id: COG1191

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789871, Length=106, Percent_Identity=37.7358490566038, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1789098, Length=119, Percent_Identity=32.7731092436975, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1789448, Length=74, Percent_Identity=41.8918918918919, Blast_Score=64, Evalue=8e-12,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR014200
- InterPro:   IPR016263
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 28266; Mature: 28266

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDRNMVVKVAMADHFQFKVMPSIKNFCLLKQGDVHYIGGTDILPAPLGASEEQDAIEALG
CCCCEEEEEEEECCEEEEECCCHHCEEEEECCCEEEECCCCCCCCCCCCCCHHHHHHHHC
TASDAEAKHTLIEHNLRLVVYIAKKFDNTGVGVEDLISIGTIGLIKAINSYDKDKNIKLA
CCCCHHHHHHHHHCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEH
TYASRCIENEILMYLRRNSKIKAEVSIDEPLNVDWDGNELLLSDIIGTDEDIIYRDIETE
HHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCEEHHHHHCCCCCCEEEECCCCH
VECKMLKRAINQLPERERIIVDLRFGLSSEDGEEKTQKEVADMLGISQSYISRLEKKIMR
HHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHH
QLRKEMVKSC
HHHHHHHHCC
>Mature Secondary Structure
MDRNMVVKVAMADHFQFKVMPSIKNFCLLKQGDVHYIGGTDILPAPLGASEEQDAIEALG
CCCCEEEEEEEECCEEEEECCCHHCEEEEECCCEEEECCCCCCCCCCCCCCHHHHHHHHC
TASDAEAKHTLIEHNLRLVVYIAKKFDNTGVGVEDLISIGTIGLIKAINSYDKDKNIKLA
CCCCHHHHHHHHHCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEH
TYASRCIENEILMYLRRNSKIKAEVSIDEPLNVDWDGNELLLSDIIGTDEDIIYRDIETE
HHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCEEHHHHHCCCCCCEEEECCCCH
VECKMLKRAINQLPERERIIVDLRFGLSSEDGEEKTQKEVADMLGISQSYISRLEKKIMR
HHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHH
QLRKEMVKSC
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6438529; 9384377; 3104904; 2106671 [H]