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The map label for this gene is murD

Identifier: 238916668

GI number: 238916668

Start: 734870

End: 736222

Strand: Direct

Name: murD

Synonym: EUBELI_00730

Alternate gene names: 238916668

Gene position: 734870-736222 (Clockwise)

Preceding gene: 238916667

Following gene: 238916669

Centisome position: 34.27

GC content: 39.02

Gene sequence:

>1353_bases
ATGGATTTAAAGAATAAGACAGTAATGGTCGTGGGAACAGGCATAAGCGGTATTGGAGCCGTTGACCTTTTGAATAAAGT
TGGTGCTGACTGCATACTGTATGATGGCAATGAAAAGCTTGACAGACAGAAGGTGCAGGAGAAGTTAGGTGATAACAAGG
CAGAGATTATCATCGGGGCATTTGATGAGGCACTTCTTTCGAAGATTGATCTGCTTGTAATAAGTCCGGGGGTTCCTATT
GACAGTCCGATAGTGCTTACATTTAAGAATGCCGGTATCCCTGTATGGGGCGAGATTGAGCTTGCATATAATTATGATAA
GGGTAAGATTATTGCAATTACAGGAACTAACGGAAAGACAACAACTACAGCGCTTGTTGGACAGATAATTGCTGCATATA
ATGAGAAAACATTTGTCGTTGGTAATATAGGTAATTCATATACAGGTGAAGTGTTAAAGACAAGTGAGGATTCATATACA
GTTGCTGAGATAAGCAGCTTCCAGTTAGAGACAGTACATGAATTCCATCCGATTGTATCTGCAATTCTTAATATTACACC
TGACCATCTTAACAGACACCATACAATGGAATGCTATGCATGGACCAAGGAAAGAATAAGTGAGAACCAGACTAAGGCAG
ATACATGTGTGCTTAATCTTGAGGATAAGTATCTTACAGACTTTGCACCTGAGTGTAAGGCAAATGTTGTGTGGTTTTCG
AGTGAAAGAAAGCCATCAGTCGGAGCATATGTTGATGGCGAGATGATTAAGTATACAGACGGAACTAATGATTACGACAT
GTTAAATGTCCATGACATGAATCTTTTAGGAAAGCATAACTATGAAAATGTATGCGCAGCAATTGCAATGACTAAGGCTG
CCGGAATTCCTGATGATATTATAATAGAACAGGTTAAGAAATTTAAGGCTGTTGAACACAGAATTGAATATGTTGCAACA
AAGAATGGCGTTGATTACTATAATGATTCCAAGGGAACTAACCCTGAGGCAGCAGTTAAGGCTATTGAAGCTATGGTTAA
GCCTACAATTCTTATCGGCGGAGGTTATGATAAAGGCTCAGAATTCGACCTTTATGTAAAGGCATTTAAGGATAAGGTTA
AGCTTCTTGTGCTTATAGGACAGACAAGTGCCAAGATTGCTGATACATGTAAGAAATATGGCTTTGAGAATATCGAATAT
GCCGATTCAATGGAACAGGTAGTAGATATATGTGCTAAAAATGCGGTAAGTGGAGATGCGGTTCTTTTATCTCCTGCATG
TGCAAGCTGGGGAATGTTCGATAACTACGAACAGAGAGGAAGAATATTCAAGGATCTTGTTAATAATCTGTAA

Upstream 100 bases:

>100_bases
GCCGTATATTGGTATAGAAAGGTCAGTTCAACTTGAAAAAAACGATGATTGATAGTAATATATTTTTGTATACATTTTGT
ATGAAAGGAAGTAAACGACG

Downstream 100 bases:

>100_bases
AAAGGCGGTGCTATATGCCGGGGAATAATAGAAATAGAAGAAATAACAGAAATAAAAAAAAGAGAAAACAATATGGATTT
TACTTTGATTACACTCTTCT

Product: UDP-N-acetylmuramoylalanine--D-glutamate ligase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Number of amino acids: Translated: 450; Mature: 450

Protein sequence:

>450_residues
MDLKNKTVMVVGTGISGIGAVDLLNKVGADCILYDGNEKLDRQKVQEKLGDNKAEIIIGAFDEALLSKIDLLVISPGVPI
DSPIVLTFKNAGIPVWGEIELAYNYDKGKIIAITGTNGKTTTTALVGQIIAAYNEKTFVVGNIGNSYTGEVLKTSEDSYT
VAEISSFQLETVHEFHPIVSAILNITPDHLNRHHTMECYAWTKERISENQTKADTCVLNLEDKYLTDFAPECKANVVWFS
SERKPSVGAYVDGEMIKYTDGTNDYDMLNVHDMNLLGKHNYENVCAAIAMTKAAGIPDDIIIEQVKKFKAVEHRIEYVAT
KNGVDYYNDSKGTNPEAAVKAIEAMVKPTILIGGGYDKGSEFDLYVKAFKDKVKLLVLIGQTSAKIADTCKKYGFENIEY
ADSMEQVVDICAKNAVSGDAVLLSPACASWGMFDNYEQRGRIFKDLVNNL

Sequences:

>Translated_450_residues
MDLKNKTVMVVGTGISGIGAVDLLNKVGADCILYDGNEKLDRQKVQEKLGDNKAEIIIGAFDEALLSKIDLLVISPGVPI
DSPIVLTFKNAGIPVWGEIELAYNYDKGKIIAITGTNGKTTTTALVGQIIAAYNEKTFVVGNIGNSYTGEVLKTSEDSYT
VAEISSFQLETVHEFHPIVSAILNITPDHLNRHHTMECYAWTKERISENQTKADTCVLNLEDKYLTDFAPECKANVVWFS
SERKPSVGAYVDGEMIKYTDGTNDYDMLNVHDMNLLGKHNYENVCAAIAMTKAAGIPDDIIIEQVKKFKAVEHRIEYVAT
KNGVDYYNDSKGTNPEAAVKAIEAMVKPTILIGGGYDKGSEFDLYVKAFKDKVKLLVLIGQTSAKIADTCKKYGFENIEY
ADSMEQVVDICAKNAVSGDAVLLSPACASWGMFDNYEQRGRIFKDLVNNL
>Mature_450_residues
MDLKNKTVMVVGTGISGIGAVDLLNKVGADCILYDGNEKLDRQKVQEKLGDNKAEIIIGAFDEALLSKIDLLVISPGVPI
DSPIVLTFKNAGIPVWGEIELAYNYDKGKIIAITGTNGKTTTTALVGQIIAAYNEKTFVVGNIGNSYTGEVLKTSEDSYT
VAEISSFQLETVHEFHPIVSAILNITPDHLNRHHTMECYAWTKERISENQTKADTCVLNLEDKYLTDFAPECKANVVWFS
SERKPSVGAYVDGEMIKYTDGTNDYDMLNVHDMNLLGKHNYENVCAAIAMTKAAGIPDDIIIEQVKKFKAVEHRIEYVAT
KNGVDYYNDSKGTNPEAAVKAIEAMVKPTILIGGGYDKGSEFDLYVKAFKDKVKLLVLIGQTSAKIADTCKKYGFENIEY
ADSMEQVVDICAKNAVSGDAVLLSPACASWGMFDNYEQRGRIFKDLVNNL

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA)

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family

Homologues:

Organism=Escherichia coli, GI1786276, Length=462, Percent_Identity=33.5497835497836, Blast_Score=192, Evalue=5e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURD_EUBE2 (C4Z534)

Other databases:

- EMBL:   CP001104
- RefSeq:   YP_002930185.1
- ProteinModelPortal:   C4Z534
- GeneID:   7957275
- GenomeReviews:   CP001104_GR
- KEGG:   eel:EUBELI_00730
- OMA:   VKYYNDS
- ProtClustDB:   CLSK2506243
- GO:   GO:0005737
- HAMAP:   MF_00639
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762
- Gene3D:   G3DSA:3.90.190.20
- Gene3D:   G3DSA:3.40.1190.10
- Gene3D:   G3DSA:3.40.50.720
- TIGRFAMs:   TIGR01087

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M; SSF53244 Mur_ligase_C; SSF53623 Mur_ligase_cen

EC number: =6.3.2.9

Molecular weight: Translated: 49440; Mature: 49440

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLKNKTVMVVGTGISGIGAVDLLNKVGADCILYDGNEKLDRQKVQEKLGDNKAEIIIGA
CCCCCCEEEEEECCCCCCHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHCCCCEEEEEEE
FDEALLSKIDLLVISPGVPIDSPIVLTFKNAGIPVWGEIELAYNYDKGKIIAITGTNGKT
HHHHHHCCCCEEEECCCCCCCCCEEEEEECCCCCEEEEEEEEEEECCCEEEEEECCCCCH
TTTALVGQIIAAYNEKTFVVGNIGNSYTGEVLKTSEDSYTVAEISSFQLETVHEFHPIVS
HHHHHHHHHHHHCCCCEEEEECCCCCCCCCEEECCCCCEEEEECCCHHHHHHHHHHHHHH
AILNITPDHLNRHHTMECYAWTKERISENQTKADTCVLNLEDKYLTDFAPECKANVVWFS
HHHHCCHHHCCCCCCEEEEEECHHHHCCCCCCCCEEEEECCHHHHHCCCCCCCCEEEEEE
SERKPSVGAYVDGEMIKYTDGTNDYDMLNVHDMNLLGKHNYENVCAAIAMTKAAGIPDDI
CCCCCCCCEEECCCEEEEECCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCHHH
IIEQVKKFKAVEHRIEYVATKNGVDYYNDSKGTNPEAAVKAIEAMVKPTILIGGGYDKGS
HHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCEEEEECCCCCCC
EFDLYVKAFKDKVKLLVLIGQTSAKIADTCKKYGFENIEYADSMEQVVDICAKNAVSGDA
CEEHHHHHHHCCEEEEEEECCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCE
VLLSPACASWGMFDNYEQRGRIFKDLVNNL
EEEECCCCCCCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MDLKNKTVMVVGTGISGIGAVDLLNKVGADCILYDGNEKLDRQKVQEKLGDNKAEIIIGA
CCCCCCEEEEEECCCCCCHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHCCCCEEEEEEE
FDEALLSKIDLLVISPGVPIDSPIVLTFKNAGIPVWGEIELAYNYDKGKIIAITGTNGKT
HHHHHHCCCCEEEECCCCCCCCCEEEEEECCCCCEEEEEEEEEEECCCEEEEEECCCCCH
TTTALVGQIIAAYNEKTFVVGNIGNSYTGEVLKTSEDSYTVAEISSFQLETVHEFHPIVS
HHHHHHHHHHHHCCCCEEEEECCCCCCCCCEEECCCCCEEEEECCCHHHHHHHHHHHHHH
AILNITPDHLNRHHTMECYAWTKERISENQTKADTCVLNLEDKYLTDFAPECKANVVWFS
HHHHCCHHHCCCCCCEEEEEECHHHHCCCCCCCCEEEEECCHHHHHCCCCCCCCEEEEEE
SERKPSVGAYVDGEMIKYTDGTNDYDMLNVHDMNLLGKHNYENVCAAIAMTKAAGIPDDI
CCCCCCCCEEECCCEEEEECCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCHHH
IIEQVKKFKAVEHRIEYVATKNGVDYYNDSKGTNPEAAVKAIEAMVKPTILIGGGYDKGS
HHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCEEEEECCCCCCC
EFDLYVKAFKDKVKLLVLIGQTSAKIADTCKKYGFENIEYADSMEQVVDICAKNAVSGDA
CEEHHHHHHHCCEEEEEEECCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCE
VLLSPACASWGMFDNYEQRGRIFKDLVNNL
EEEECCCCCCCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA