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The map label for this gene is priA [H]

Identifier: 238916652

GI number: 238916652

Start: 716532

End: 718748

Strand: Direct

Name: priA [H]

Synonym: EUBELI_00714

Alternate gene names: 238916652

Gene position: 716532-718748 (Clockwise)

Preceding gene: 238916651

Following gene: 238916653

Centisome position: 33.42

GC content: 40.82

Gene sequence:

>2217_bases
ATGTACGCAGATGTAATAATAGACATTTCACATGAACAGCTTGATAAGACATTCCAGTATGCAGTTCCGGATGAATTGGC
GGATGTAATTGAACTGGGAATGTCAGTTGATATTCCTTTTGGAGCAGGAAACAGGCAGATAACAGGGTATGTTGTAGGTT
TGGGAGACAAGGCGGCATATCCTGTTGAAAAGATAAAATATATTACAGGGATTACCGAGGGAAAGGTCACTGCAGTAAGC
AGAATGTTAAAGCTTGCGGCTTGGCTGAAGCACAATTATGGATGTACCATGAATCAGGCAATTAAGACGGTTATTCCTGT
AAAAGATAAGGTTAAACAGAAGGAAAAACGCTCGGTCAATCTTATTATTGACACGGCACAGGCGCAGAAGTATCTTGATG
TATTTGCAAAGAAAAATGCAAAAGCAAGATACAGACTGTTAGAGGTACTGATTAAGGAACCTGTAATAGATGAAAGCATT
ATTAAATCCAAGCTTAATATCACGGCACAGACGGTAAAAGCATTTGAAGATATGGGGATTGCCGAAGTCAGGTCAGAAGA
TATGTATAGGAATCCTGTAAGAAATGTTTCGGGAGAAAGAAAAAATATCGAGCTTAACAAGCAGCAAAAAAAAGCTGTGG
ATACAATAATAGCTGACTATGATAAGGGGGATTATAAGACATATCTGCTTCATGGTGTTACAGGAAGCGGAAAGACCGAG
GTGTATCTTGCGGCAATTGAGCATGTCCTTAATCATGGAAGACAGGCAATTGTGCTTATACCGGAGATTGCACTTACATT
CCAGACTGTGCAGAGGTTTTACCACAGATTCGGGGATAAGGTATCTATTATGAATTCCCGTATGTCAAAGGGAGAACGTT
ATGACCAGTTTCTGCGGGCGCAGAGAGGTGATATAAGTGTAATGATTGGACCACGTTCAGCGTTGTTCACACCGTTTTCA
AATATAGGAATTATAATAATAGATGAAGAGCATGAAGGAGCTTACAAGTCAGAGACTGTTCCAAGATACCATGCAAGAGA
GGTTGCATGCCATATGGCAAAGGAAGCAGGTGCTTCAGTGATACTTGGTTCTGCGACTCCTTCAATGGAAAGCTACTATG
CGGCAATGAATGGACATATACAGCTTATTACACTTGACAGCCGCGCAGGAAGCGGAGAGCTTCCATCAGTTGATATTGTT
GACCTGAGAGAAGAATTAAGGCTTGGAAACAGGACGATATTCAGCAACAGGTTAAGAGAACTGATGAGTGACAGACTTGC
AAAGCATGAGCAGATAATGCTTTTCCTTAATAAGAGAGGCGTGGCTGGCTTTATATCATGCCGTTCATGCGGAAAGGTTA
TGAAATGCCCGCACTGTGATGTTTCACTTACGGAGCATGCTGATGGAAGGCTGATGTGTCATTACTGCGGATATACAACA
CCTAAGATAACTGTATGCCCTTCATGCGGTTCAAGGTATGTTTCGGGTTTCAGGGCAGGAACGGAAGGTGTTGAAGCACA
GGTTAAGAAAACATTTCCACAGGCAAGAGTGCTAAGAATGGATATGGACACTACAAGAGGCAAGGATGGACATGAAAAGA
TTCTGTCGGAATTCGCCAATGGTAATGCGGATATTCTTATTGGAACTCAGATGATTGTTAAAGGACATGATTTTCCAAAT
GTAACGCTTATGGGAGTGCTTGCGGCAGACATGTCACTTTATTCCAGTGATTACAGGGCTTCTGAGAGAACTTTCCAGCT
TCTTACACAGGCGGCTGGAAGGGCAGGAAGAGCAGAAAAAAGCGGCAATGTAGTTATACAGACTTATACGCCGGAGCATT
TTAGTATTGTTAGTGCGGCTAATCAGGATTATAATGCTTTCTATGAGCAGGAAATTGCTTACAGAAAGCTGTGTGGATAT
CCGCCGGCGGATAATCTTATGAAGATTATGTTATCTTCACCAGATGAGATGTTATTGACAAAGAGTGCAGCGTGGGTTAA
AGCATTTGTGGATAACAACTGCAAGTATAAAGGACTTATGTGTATAGGTCCGGCAGATGCACCTATATATAAGATTAAAG
ATGTGTATAGTAAGATAATCTATGTAAAACATAAAGACAGGGAAGTTCTTAACAGTATATATGAAAAGCTTGATGTTAAT
ATTGTTGGAAATCAGGCTTTTAAGAATATTAATGTACAATACGATATTAATGGATGA

Upstream 100 bases:

>100_bases
GTAAAGGTCATGAGGATTATCAGGAGATTAAGGGTGTGAAGTATCCTATGGATGAGAGGGTGCTTATCAAAGAAGTGCTT
GAAGAATTGAAGGGTAATTA

Downstream 100 bases:

>100_bases
TTGCGAGGAGGAATATATGGCAATAAGAAATATTAGAACACTTGGAGATGATATACTAAGAGCCAAGGCAAAAGAGATTA
CAGAGATGACACCAAGAATT

Product: primosomal protein N

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 738; Mature: 738

Protein sequence:

>738_residues
MYADVIIDISHEQLDKTFQYAVPDELADVIELGMSVDIPFGAGNRQITGYVVGLGDKAAYPVEKIKYITGITEGKVTAVS
RMLKLAAWLKHNYGCTMNQAIKTVIPVKDKVKQKEKRSVNLIIDTAQAQKYLDVFAKKNAKARYRLLEVLIKEPVIDESI
IKSKLNITAQTVKAFEDMGIAEVRSEDMYRNPVRNVSGERKNIELNKQQKKAVDTIIADYDKGDYKTYLLHGVTGSGKTE
VYLAAIEHVLNHGRQAIVLIPEIALTFQTVQRFYHRFGDKVSIMNSRMSKGERYDQFLRAQRGDISVMIGPRSALFTPFS
NIGIIIIDEEHEGAYKSETVPRYHAREVACHMAKEAGASVILGSATPSMESYYAAMNGHIQLITLDSRAGSGELPSVDIV
DLREELRLGNRTIFSNRLRELMSDRLAKHEQIMLFLNKRGVAGFISCRSCGKVMKCPHCDVSLTEHADGRLMCHYCGYTT
PKITVCPSCGSRYVSGFRAGTEGVEAQVKKTFPQARVLRMDMDTTRGKDGHEKILSEFANGNADILIGTQMIVKGHDFPN
VTLMGVLAADMSLYSSDYRASERTFQLLTQAAGRAGRAEKSGNVVIQTYTPEHFSIVSAANQDYNAFYEQEIAYRKLCGY
PPADNLMKIMLSSPDEMLLTKSAAWVKAFVDNNCKYKGLMCIGPADAPIYKIKDVYSKIIYVKHKDREVLNSIYEKLDVN
IVGNQAFKNINVQYDING

Sequences:

>Translated_738_residues
MYADVIIDISHEQLDKTFQYAVPDELADVIELGMSVDIPFGAGNRQITGYVVGLGDKAAYPVEKIKYITGITEGKVTAVS
RMLKLAAWLKHNYGCTMNQAIKTVIPVKDKVKQKEKRSVNLIIDTAQAQKYLDVFAKKNAKARYRLLEVLIKEPVIDESI
IKSKLNITAQTVKAFEDMGIAEVRSEDMYRNPVRNVSGERKNIELNKQQKKAVDTIIADYDKGDYKTYLLHGVTGSGKTE
VYLAAIEHVLNHGRQAIVLIPEIALTFQTVQRFYHRFGDKVSIMNSRMSKGERYDQFLRAQRGDISVMIGPRSALFTPFS
NIGIIIIDEEHEGAYKSETVPRYHAREVACHMAKEAGASVILGSATPSMESYYAAMNGHIQLITLDSRAGSGELPSVDIV
DLREELRLGNRTIFSNRLRELMSDRLAKHEQIMLFLNKRGVAGFISCRSCGKVMKCPHCDVSLTEHADGRLMCHYCGYTT
PKITVCPSCGSRYVSGFRAGTEGVEAQVKKTFPQARVLRMDMDTTRGKDGHEKILSEFANGNADILIGTQMIVKGHDFPN
VTLMGVLAADMSLYSSDYRASERTFQLLTQAAGRAGRAEKSGNVVIQTYTPEHFSIVSAANQDYNAFYEQEIAYRKLCGY
PPADNLMKIMLSSPDEMLLTKSAAWVKAFVDNNCKYKGLMCIGPADAPIYKIKDVYSKIIYVKHKDREVLNSIYEKLDVN
IVGNQAFKNINVQYDING
>Mature_738_residues
MYADVIIDISHEQLDKTFQYAVPDELADVIELGMSVDIPFGAGNRQITGYVVGLGDKAAYPVEKIKYITGITEGKVTAVS
RMLKLAAWLKHNYGCTMNQAIKTVIPVKDKVKQKEKRSVNLIIDTAQAQKYLDVFAKKNAKARYRLLEVLIKEPVIDESI
IKSKLNITAQTVKAFEDMGIAEVRSEDMYRNPVRNVSGERKNIELNKQQKKAVDTIIADYDKGDYKTYLLHGVTGSGKTE
VYLAAIEHVLNHGRQAIVLIPEIALTFQTVQRFYHRFGDKVSIMNSRMSKGERYDQFLRAQRGDISVMIGPRSALFTPFS
NIGIIIIDEEHEGAYKSETVPRYHAREVACHMAKEAGASVILGSATPSMESYYAAMNGHIQLITLDSRAGSGELPSVDIV
DLREELRLGNRTIFSNRLRELMSDRLAKHEQIMLFLNKRGVAGFISCRSCGKVMKCPHCDVSLTEHADGRLMCHYCGYTT
PKITVCPSCGSRYVSGFRAGTEGVEAQVKKTFPQARVLRMDMDTTRGKDGHEKILSEFANGNADILIGTQMIVKGHDFPN
VTLMGVLAADMSLYSSDYRASERTFQLLTQAAGRAGRAEKSGNVVIQTYTPEHFSIVSAANQDYNAFYEQEIAYRKLCGY
PPADNLMKIMLSSPDEMLLTKSAAWVKAFVDNNCKYKGLMCIGPADAPIYKIKDVYSKIIYVKHKDREVLNSIYEKLDVN
IVGNQAFKNINVQYDING

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=634, Percent_Identity=35.8044164037855, Blast_Score=404, Evalue=1e-113,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C [H]

EC number: NA

Molecular weight: Translated: 82501; Mature: 82501

Theoretical pI: Translated: 8.68; Mature: 8.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYADVIIDISHEQLDKTFQYAVPDELADVIELGMSVDIPFGAGNRQITGYVVGLGDKAAY
CCCEEEEECCHHHHHHHHHHCCCHHHHHHHHCCCEEECCCCCCCCEEEEEEEECCCCCCC
PVEKIKYITGITEGKVTAVSRMLKLAAWLKHNYGCTMNQAIKTVIPVKDKVKQKEKRSVN
CHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHCCCEE
LIIDTAQAQKYLDVFAKKNAKARYRLLEVLIKEPVIDESIIKSKLNITAQTVKAFEDMGI
EEEECHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHCHHHHHHHHHHHCCH
AEVRSEDMYRNPVRNVSGERKNIELNKQQKKAVDTIIADYDKGDYKTYLLHGVTGSGKTE
HHHHHHHHHHCHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCH
VYLAAIEHVLNHGRQAIVLIPEIALTFQTVQRFYHRFGDKVSIMNSRMSKGERYDQFLRA
HHHHHHHHHHHCCCEEEEEECHHHHHHHHHHHHHHHHCCEEEEHHHHHHCCHHHHHHHHH
QRGDISVMIGPRSALFTPFSNIGIIIIDEEHEGAYKSETVPRYHAREVACHMAKEAGASV
CCCCEEEEECCCHHHCCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEE
ILGSATPSMESYYAAMNGHIQLITLDSRAGSGELPSVDIVDLREELRLGNRTIFSNRLRE
EEECCCCCHHHHHHHHCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHH
LMSDRLAKHEQIMLFLNKRGVAGFISCRSCGKVMKCPHCDVSLTEHADGRLMCHYCGYTT
HHHHHHCCCCEEEEEEECCCCEEEEEHHHCCHHHCCCCCCCEEECCCCCCEEEEECCCCC
PKITVCPSCGSRYVSGFRAGTEGVEAQVKKTFPQARVLRMDMDTTRGKDGHEKILSEFAN
CCEEECCCCCHHHHHHHCCCCCHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHCC
GNADILIGTQMIVKGHDFPNVTLMGVLAADMSLYSSDYRASERTFQLLTQAAGRAGRAEK
CCCEEEEEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCC
SGNVVIQTYTPEHFSIVSAANQDYNAFYEQEIAYRKLCGYPPADNLMKIMLSSPDEMLLT
CCCEEEEEECCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCHHEEE
KSAAWVKAFVDNNCKYKGLMCIGPADAPIYKIKDVYSKIIYVKHKDREVLNSIYEKLDVN
CHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHEEEEECCCHHHHHHHHHHHCEE
IVGNQAFKNINVQYDING
EECCHHHCCCEEEEEECC
>Mature Secondary Structure
MYADVIIDISHEQLDKTFQYAVPDELADVIELGMSVDIPFGAGNRQITGYVVGLGDKAAY
CCCEEEEECCHHHHHHHHHHCCCHHHHHHHHCCCEEECCCCCCCCEEEEEEEECCCCCCC
PVEKIKYITGITEGKVTAVSRMLKLAAWLKHNYGCTMNQAIKTVIPVKDKVKQKEKRSVN
CHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHCCCEE
LIIDTAQAQKYLDVFAKKNAKARYRLLEVLIKEPVIDESIIKSKLNITAQTVKAFEDMGI
EEEECHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHCHHHHHHHHHHHCCH
AEVRSEDMYRNPVRNVSGERKNIELNKQQKKAVDTIIADYDKGDYKTYLLHGVTGSGKTE
HHHHHHHHHHCHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCH
VYLAAIEHVLNHGRQAIVLIPEIALTFQTVQRFYHRFGDKVSIMNSRMSKGERYDQFLRA
HHHHHHHHHHHCCCEEEEEECHHHHHHHHHHHHHHHHCCEEEEHHHHHHCCHHHHHHHHH
QRGDISVMIGPRSALFTPFSNIGIIIIDEEHEGAYKSETVPRYHAREVACHMAKEAGASV
CCCCEEEEECCCHHHCCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEE
ILGSATPSMESYYAAMNGHIQLITLDSRAGSGELPSVDIVDLREELRLGNRTIFSNRLRE
EEECCCCCHHHHHHHHCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHH
LMSDRLAKHEQIMLFLNKRGVAGFISCRSCGKVMKCPHCDVSLTEHADGRLMCHYCGYTT
HHHHHHCCCCEEEEEEECCCCEEEEEHHHCCHHHCCCCCCCEEECCCCCCEEEEECCCCC
PKITVCPSCGSRYVSGFRAGTEGVEAQVKKTFPQARVLRMDMDTTRGKDGHEKILSEFAN
CCEEECCCCCHHHHHHHCCCCCHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHCC
GNADILIGTQMIVKGHDFPNVTLMGVLAADMSLYSSDYRASERTFQLLTQAAGRAGRAEK
CCCEEEEEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCC
SGNVVIQTYTPEHFSIVSAANQDYNAFYEQEIAYRKLCGYPPADNLMKIMLSSPDEMLLT
CCCEEEEEECCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCHHEEE
KSAAWVKAFVDNNCKYKGLMCIGPADAPIYKIKDVYSKIIYVKHKDREVLNSIYEKLDVN
CHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHEEEEECCCHHHHHHHHHHHCEE
IVGNQAFKNINVQYDING
EECCHHHCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9534248; 9384377; 9086272 [H]