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The map label for this gene is murE [H]

Identifier: 238916651

GI number: 238916651

Start: 715039

End: 716532

Strand: Direct

Name: murE [H]

Synonym: EUBELI_00713

Alternate gene names: 238916651

Gene position: 715039-716532 (Clockwise)

Preceding gene: 238916650

Following gene: 238916652

Centisome position: 33.35

GC content: 39.02

Gene sequence:

>1494_bases
ATGAAGCTTTCAGAGATATTAAAAATATGTTCAGAAAATGAAACTGCAGACTTCAGATACAGTGTTGTATGCGGTGATAC
TGATGTGGATGTTACTGGAATTACATCTGATTCAAGAAAAATGCAGGATGGATATGCATTTGTATGCATTAAGGGTGCTG
TTAGTGATGGGCATAAGTATATTGACCAGATTAAGGATAAGGCATCTGTAATTGTTGTTCTTGATGGCGAATACGAAGCC
AAAGACTGCAAGGCAGCAGTTGTAAGCACAGATAACACAAGGCTTGCGCTTGCACTTATGTCGGCAGCAGTCTATGGCAA
TCCTGACAGGAAGCTGTTTACAATAGGAATAACAGGAACTAAGGGAAAGACAACAACCACTTATATGGTTAAAAATGTGC
TTGAGGCATGCGGCATTAAGACTGGACTTATAGGAACAATTGAGACTATTATCGGGGATGAAGCGATTCCGGCACATAAT
ACAACACCTGAATCAATAGAGATTCACCAGTCATTTGCAAAGATGGTAGACGCTGGCTGTAAAGCAGTTGTTATGGAGGT
GTCTTCGCAGGGACTTAAGCTTGACAGAACAGCAGGTATTATGTTCGATATAGGAGTGTTTACTAATCTTGAGCCCGACC
ATATCGGACCTAATGAGCATGAGTCATTTGAGGATTATCTTAATTGTAAGGCTAAGTTATTCAAGCAGTGCAAGGTTGGT
ATCGTTAATGCTGATGATAAGCATACCCAGGATATCCTTAGGGGAGCAATATGTAAGGTTGAAAGCTATGGTATCGGAGA
TAATGCTGATATTAAGGCTGAAAATATTGAACTTTTACATGAACCGGGTAAAATTGGTCTTACATATGACTGTACAGGAC
TTGTTAATATGAAGGTTGAACTTAATCTTCCGGGTAAGTTTTCTGTATACAATTCATTATGTGCAATAGCAATTACAAGG
CATTTCGATGTTGACGAAGAGGCACTTAAGGAAACTCTTAAGCATGTTAAGGTTAAGGGAAGAATTGAGCTTGTAAAGGT
TTCAGATGATTTTACATTAATGATAGATTATGCTCATAATGCAATGGCTTTAGAGAGTATACTTACAACTCTTAAGGAAT
ATCACCCTCACAGACTTGTATGCCTGTTTGGATGTGGTGGTAACCGCTCCAAGGAAAGAAGATATGAGATGGGCGAGGTT
TCAGGAAAACTTGCTGACCTTACTATTATCACATCAGATAATCCAAGATTTGAAGAGCCGGAGGCAATTATAGAAGATAT
CAAGACAGGAATTGCTAAGACTACCGGAAAACATGTTGATATTACAGACAGAAAAGAAGCTATTAAGTATGCGATAGAAC
ATGGCGAACCGGGTGATATTATTGTTCTTGCGGGTAAAGGTCATGAGGATTATCAGGAGATTAAGGGTGTGAAGTATCCT
ATGGATGAGAGGGTGCTTATCAAAGAAGTGCTTGAAGAATTGAAGGGTAATTAA

Upstream 100 bases:

>100_bases
CTAAGCCAAGAAAGGTTGAGATGACAATGGAAGAGTTTGAAACACTTCTAAAGCAGCAGTAATATAAGTAATATATACTA
TAAAATAAGGAGACTAATGT

Downstream 100 bases:

>100_bases
TGTACGCAGATGTAATAATAGACATTTCACATGAACAGCTTGATAAGACATTCCAGTATGCAGTTCCGGATGAATTGGCG
GATGTAATTGAACTGGGAAT

Product: UDP-N-acetylmuramoylalanyl-D-glutamate--2, 6-diaminopimelate ligase

Products: NA

Alternate protein names: Meso-A2pm-adding enzyme; Meso-diaminopimelate-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase [H]

Number of amino acids: Translated: 497; Mature: 497

Protein sequence:

>497_residues
MKLSEILKICSENETADFRYSVVCGDTDVDVTGITSDSRKMQDGYAFVCIKGAVSDGHKYIDQIKDKASVIVVLDGEYEA
KDCKAAVVSTDNTRLALALMSAAVYGNPDRKLFTIGITGTKGKTTTTYMVKNVLEACGIKTGLIGTIETIIGDEAIPAHN
TTPESIEIHQSFAKMVDAGCKAVVMEVSSQGLKLDRTAGIMFDIGVFTNLEPDHIGPNEHESFEDYLNCKAKLFKQCKVG
IVNADDKHTQDILRGAICKVESYGIGDNADIKAENIELLHEPGKIGLTYDCTGLVNMKVELNLPGKFSVYNSLCAIAITR
HFDVDEEALKETLKHVKVKGRIELVKVSDDFTLMIDYAHNAMALESILTTLKEYHPHRLVCLFGCGGNRSKERRYEMGEV
SGKLADLTIITSDNPRFEEPEAIIEDIKTGIAKTTGKHVDITDRKEAIKYAIEHGEPGDIIVLAGKGHEDYQEIKGVKYP
MDERVLIKEVLEELKGN

Sequences:

>Translated_497_residues
MKLSEILKICSENETADFRYSVVCGDTDVDVTGITSDSRKMQDGYAFVCIKGAVSDGHKYIDQIKDKASVIVVLDGEYEA
KDCKAAVVSTDNTRLALALMSAAVYGNPDRKLFTIGITGTKGKTTTTYMVKNVLEACGIKTGLIGTIETIIGDEAIPAHN
TTPESIEIHQSFAKMVDAGCKAVVMEVSSQGLKLDRTAGIMFDIGVFTNLEPDHIGPNEHESFEDYLNCKAKLFKQCKVG
IVNADDKHTQDILRGAICKVESYGIGDNADIKAENIELLHEPGKIGLTYDCTGLVNMKVELNLPGKFSVYNSLCAIAITR
HFDVDEEALKETLKHVKVKGRIELVKVSDDFTLMIDYAHNAMALESILTTLKEYHPHRLVCLFGCGGNRSKERRYEMGEV
SGKLADLTIITSDNPRFEEPEAIIEDIKTGIAKTTGKHVDITDRKEAIKYAIEHGEPGDIIVLAGKGHEDYQEIKGVKYP
MDERVLIKEVLEELKGN
>Mature_497_residues
MKLSEILKICSENETADFRYSVVCGDTDVDVTGITSDSRKMQDGYAFVCIKGAVSDGHKYIDQIKDKASVIVVLDGEYEA
KDCKAAVVSTDNTRLALALMSAAVYGNPDRKLFTIGITGTKGKTTTTYMVKNVLEACGIKTGLIGTIETIIGDEAIPAHN
TTPESIEIHQSFAKMVDAGCKAVVMEVSSQGLKLDRTAGIMFDIGVFTNLEPDHIGPNEHESFEDYLNCKAKLFKQCKVG
IVNADDKHTQDILRGAICKVESYGIGDNADIKAENIELLHEPGKIGLTYDCTGLVNMKVELNLPGKFSVYNSLCAIAITR
HFDVDEEALKETLKHVKVKGRIELVKVSDDFTLMIDYAHNAMALESILTTLKEYHPHRLVCLFGCGGNRSKERRYEMGEV
SGKLADLTIITSDNPRFEEPEAIIEDIKTGIAKTTGKHVDITDRKEAIKYAIEHGEPGDIIVLAGKGHEDYQEIKGVKYP
MDERVLIKEVLEELKGN

Specific function: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan [H]

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786273, Length=473, Percent_Identity=31.7124735729387, Blast_Score=186, Evalue=2e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005761 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.13 [H]

Molecular weight: Translated: 54665; Mature: 54665

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: PS01011 FOLYLPOLYGLU_SYNT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLSEILKICSENETADFRYSVVCGDTDVDVTGITSDSRKMQDGYAFVCIKGAVSDGHKY
CCHHHHHHHHCCCCCCCEEEEEEECCCCCEEEECCCCCCCCCCCEEEEEEECCCCCHHHH
IDQIKDKASVIVVLDGEYEAKDCKAAVVSTDNTRLALALMSAAVYGNPDRKLFTIGITGT
HHHHCCCCCEEEEECCCCCCCCCCEEEEECCCCEEHHHHHHHHHCCCCCCEEEEEEEECC
KGKTTTTYMVKNVLEACGIKTGLIGTIETIIGDEAIPAHNTTPESIEIHQSFAKMVDAGC
CCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCC
KAVVMEVSSQGLKLDRTAGIMFDIGVFTNLEPDHIGPNEHESFEDYLNCKAKLFKQCKVG
CEEEEEHHCCCCEEECCCCEEEEECEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCE
IVNADDKHTQDILRGAICKVESYGIGDNADIKAENIELLHEPGKIGLTYDCTGLVNMKVE
EECCCCHHHHHHHHHHHHEEEECCCCCCCCEEECCEEEEECCCCEEEEEECCEEEEEEEE
LNLPGKFSVYNSLCAIAITRHFDVDEEALKETLKHVKVKGRIELVKVSDDFTLMIDYAHN
EECCCCHHHHHHHEEEEEEECCCCCHHHHHHHHHHCEECCEEEEEEECCCEEEEEECCCC
AMALESILTTLKEYHPHRLVCLFGCGGNRSKERRYEMGEVSGKLADLTIITSDNPRFEEP
HHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHCCCCEEEEEEEECCCCCCCCH
EAIIEDIKTGIAKTTGKHVDITDRKEAIKYAIEHGEPGDIIVLAGKGHEDYQEIKGVKYP
HHHHHHHHHHHHHCCCCEECCCCHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHCCCCCC
MDERVLIKEVLEELKGN
CCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MKLSEILKICSENETADFRYSVVCGDTDVDVTGITSDSRKMQDGYAFVCIKGAVSDGHKY
CCHHHHHHHHCCCCCCCEEEEEEECCCCCEEEECCCCCCCCCCCEEEEEEECCCCCHHHH
IDQIKDKASVIVVLDGEYEAKDCKAAVVSTDNTRLALALMSAAVYGNPDRKLFTIGITGT
HHHHCCCCCEEEEECCCCCCCCCCEEEEECCCCEEHHHHHHHHHCCCCCCEEEEEEEECC
KGKTTTTYMVKNVLEACGIKTGLIGTIETIIGDEAIPAHNTTPESIEIHQSFAKMVDAGC
CCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCC
KAVVMEVSSQGLKLDRTAGIMFDIGVFTNLEPDHIGPNEHESFEDYLNCKAKLFKQCKVG
CEEEEEHHCCCCEEECCCCEEEEECEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCE
IVNADDKHTQDILRGAICKVESYGIGDNADIKAENIELLHEPGKIGLTYDCTGLVNMKVE
EECCCCHHHHHHHHHHHHEEEECCCCCCCCEEECCEEEEECCCCEEEEEECCEEEEEEEE
LNLPGKFSVYNSLCAIAITRHFDVDEEALKETLKHVKVKGRIELVKVSDDFTLMIDYAHN
EECCCCHHHHHHHEEEEEEECCCCCHHHHHHHHHHCEECCEEEEEEECCCEEEEEECCCC
AMALESILTTLKEYHPHRLVCLFGCGGNRSKERRYEMGEVSGKLADLTIITSDNPRFEEP
HHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHCCCCEEEEEEEECCCCCCCCH
EAIIEDIKTGIAKTTGKHVDITDRKEAIKYAIEHGEPGDIIVLAGKGHEDYQEIKGVKYP
HHHHHHHHHHHHHCCCCEECCCCHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHCCCCCC
MDERVLIKEVLEELKGN
CCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11997336 [H]