Click here to switch to the map view.

The map label for this gene is aprX [H]

Identifier: 238916644

GI number: 238916644

Start: 705429

End: 706235

Strand: Reverse

Name: aprX [H]

Synonym: EUBELI_00706

Alternate gene names: 238916644

Gene position: 706235-705429 (Counterclockwise)

Preceding gene: 238916662

Following gene: 238916639

Centisome position: 32.94

GC content: 40.4

Gene sequence:

>807_bases
ATGAATAAATTAATCACTGTTGCTGTAATAGATTCAGGAATATATCCTCATATAGATTTCAAAAACCGCATAGTAGCTTT
CAAGGATTTTGTCAATGGCAGAAAAAATGCTTATGATGACTGTGGACATGGCACCCATGTAAGTGGAATTATCGCAGGCA
GCGGCTATGCAAGCCACGGCAGAATCAAAGGAATCTGCCCTGCTGCCAATCTCGCAGGAATCAAGGTATTAGACAGACTT
GGCAATGGCAGGATTGAAAATGTTTTAGATGCCGCTGGCTGGATAATCTCCCATCAGAAGGATTTGAATATAAGAGTTGT
CAATATATCCTTCGGTGCAACCACATTTGGCGAAAAGGAAAATCTCATTGCACTTGAAAAAGCAATTGAAAAGCTCTGGG
ACAATAATATTATTGTTGTCGCAGCCGCTGGAAATGAAGGACCGGGGCGACAAACTGTTGCAATTCCCGGAACCTGCAAG
AAAATTATCACAGTTGGTTCCACGGATGGCAAAGATTTCTATTCAGGAAGAGGTCCAACCAGTGAATGTATAGTAAAACC
AGAACTTATTGTAAATGGTTCATATATCAGGTCATGCAATAATTCTGCTAACGGATATTCTGTAAAAAGCGGAACTTCCA
TGTCTGCCCCGGTTGTTTCTGGTGCAATTGCAAATGTTCTGACAAAAAAAGACATGTCTCCTAAAGATATTAAAAAAAGG
CTTCATTCCTGCTGCACTAAAAAAGCCCTGCCGGATAATCTGCAGGGCTGGGGATTATTAAATATTTCCGAATTTCTTAA
TCTCTGA

Upstream 100 bases:

>100_bases
ATGAATAATTAGTCACATCTAATCCCTGTAATATAACCGCACTAAACCACCCAATCTGAATATACTAAAATATATATATT
ATGTTCTGAAAATAATTATT

Downstream 100 bases:

>100_bases
TTACTATTTTGCACTATTAACTGCTACAAGAATATCAAGCATAAAATACTGTACAAGAATAAGGATAAGTGCAAGCAATA
CGATAACAACAAGCGGAATC

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 268; Mature: 268

Protein sequence:

>268_residues
MNKLITVAVIDSGIYPHIDFKNRIVAFKDFVNGRKNAYDDCGHGTHVSGIIAGSGYASHGRIKGICPAANLAGIKVLDRL
GNGRIENVLDAAGWIISHQKDLNIRVVNISFGATTFGEKENLIALEKAIEKLWDNNIIVVAAAGNEGPGRQTVAIPGTCK
KIITVGSTDGKDFYSGRGPTSECIVKPELIVNGSYIRSCNNSANGYSVKSGTSMSAPVVSGAIANVLTKKDMSPKDIKKR
LHSCCTKKALPDNLQGWGLLNISEFLNL

Sequences:

>Translated_268_residues
MNKLITVAVIDSGIYPHIDFKNRIVAFKDFVNGRKNAYDDCGHGTHVSGIIAGSGYASHGRIKGICPAANLAGIKVLDRL
GNGRIENVLDAAGWIISHQKDLNIRVVNISFGATTFGEKENLIALEKAIEKLWDNNIIVVAAAGNEGPGRQTVAIPGTCK
KIITVGSTDGKDFYSGRGPTSECIVKPELIVNGSYIRSCNNSANGYSVKSGTSMSAPVVSGAIANVLTKKDMSPKDIKKR
LHSCCTKKALPDNLQGWGLLNISEFLNL
>Mature_268_residues
MNKLITVAVIDSGIYPHIDFKNRIVAFKDFVNGRKNAYDDCGHGTHVSGIIAGSGYASHGRIKGICPAANLAGIKVLDRL
GNGRIENVLDAAGWIISHQKDLNIRVVNISFGATTFGEKENLIALEKAIEKLWDNNIIVVAAAGNEGPGRQTVAIPGTCK
KIITVGSTDGKDFYSGRGPTSECIVKPELIVNGSYIRSCNNSANGYSVKSGTSMSAPVVSGAIANVLTKKDMSPKDIKKR
LHSCCTKKALPDNLQGWGLLNISEFLNL

Specific function: Displays serine protease activity. Seems to have a broad substrate specificity [H]

COG id: COG1404

COG function: function code O; Subtilisin-like serine proteases

Gene ontology:

Cell location: Cytoplasm (Probable). Note=Is leaked to the culture medium during the late stationary phase owing to cell lysis [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S8 family [H]

Homologues:

Organism=Homo sapiens, GI4506775, Length=280, Percent_Identity=30, Blast_Score=83, Evalue=3e-16,
Organism=Homo sapiens, GI186972143, Length=235, Percent_Identity=31.4893617021277, Blast_Score=77, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6319893, Length=257, Percent_Identity=29.5719844357977, Blast_Score=82, Evalue=7e-17,
Organism=Saccharomyces cerevisiae, GI6324576, Length=272, Percent_Identity=26.1029411764706, Blast_Score=70, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6320775, Length=273, Percent_Identity=28.5714285714286, Blast_Score=69, Evalue=6e-13,
Organism=Drosophila melanogaster, GI45550681, Length=284, Percent_Identity=30.2816901408451, Blast_Score=73, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000209
- InterPro:   IPR022398
- InterPro:   IPR015500 [H]

Pfam domain/function: PF00082 Peptidase_S8 [H]

EC number: NA

Molecular weight: Translated: 28596; Mature: 28596

Theoretical pI: Translated: 9.41; Mature: 9.41

Prosite motif: PS00136 SUBTILASE_ASP ; PS00137 SUBTILASE_HIS ; PS00138 SUBTILASE_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKLITVAVIDSGIYPHIDFKNRIVAFKDFVNGRKNAYDDCGHGTHVSGIIAGSGYASHG
CCCEEEEEEECCCCCCCCCCCCCEEEEHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCCC
RIKGICPAANLAGIKVLDRLGNGRIENVLDAAGWIISHQKDLNIRVVNISFGATTFGEKE
CEEEECCCCCHHHHHHHHHHCCCHHHHHHHHHHHEEECCCCCEEEEEEEEECCCCCCCCC
NLIALEKAIEKLWDNNIIVVAAAGNEGPGRQTVAIPGTCKKIITVGSTDGKDFYSGRGPT
CCHHHHHHHHHHCCCCEEEEEECCCCCCCCEEEECCCCCEEEEEECCCCCCCCCCCCCCC
SECIVKPELIVNGSYIRSCNNSANGYSVKSGTSMSAPVVSGAIANVLTKKDMSPKDIKKR
HHEEECCEEEECCHHHCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHH
LHSCCTKKALPDNLQGWGLLNISEFLNL
HHHHHHHCCCCCCCCCCEEEEHHHHHCC
>Mature Secondary Structure
MNKLITVAVIDSGIYPHIDFKNRIVAFKDFVNGRKNAYDDCGHGTHVSGIIAGSGYASHG
CCCEEEEEEECCCCCCCCCCCCCEEEEHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCCC
RIKGICPAANLAGIKVLDRLGNGRIENVLDAAGWIISHQKDLNIRVVNISFGATTFGEKE
CEEEECCCCCHHHHHHHHHHCCCHHHHHHHHHHHEEECCCCCEEEEEEEEECCCCCCCCC
NLIALEKAIEKLWDNNIIVVAAAGNEGPGRQTVAIPGTCKKIITVGSTDGKDFYSGRGPT
CCHHHHHHHHHHCCCCEEEEEECCCCCCCCEEEECCCCCEEEEEECCCCCCCCCCCCCCC
SECIVKPELIVNGSYIRSCNNSANGYSVKSGTSMSAPVVSGAIANVLTKKDMSPKDIKKR
HHEEECCEEEECCHHHCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHH
LHSCCTKKALPDNLQGWGLLNISEFLNL
HHHHHHHCCCCCCCCCCEEEEHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]