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The map label for this gene is hypB [H]

Identifier: 238916641

GI number: 238916641

Start: 703884

End: 704594

Strand: Direct

Name: hypB [H]

Synonym: EUBELI_00703

Alternate gene names: 238916641

Gene position: 703884-704594 (Clockwise)

Preceding gene: 238916640

Following gene: 238916642

Centisome position: 32.83

GC content: 38.54

Gene sequence:

>711_bases
ATGAATAATAGTTATAATGAAAGAAGCATGAAGATAATTGACCTTAAGAAAACTATCTTAGAGGACAATGATGCTGATGC
TGACAGATTAAGAGAAGAGCTAAAGAAGAAAAATGTATTCTATATGAATGTCATGTCAAGTCCCGGTTCGGGTAAGACTA
CAACTATTGTTGGTACAATTGAAAGACTTAGGAAACATTTTAATATAGGTGTGATGGAAGCTGATATTGATTCTGATGTT
GATGCACTTACTGTTGCTAAGGCAGGAGTGTCAGCAGTACAGCTGCACACAGGCGGAATGTGTCATCTTGATGCTGATAT
GTCAAGACAGGGAATGGCTGCGTTAGAAGCTGAAGCAGATAATGCACTTGATTTTATTATACTCGAGAACGTTGGTAATC
TTGTGTGTCCGGCTGAGTTTGATACAGGCGCATGTCTTAATGTGGCAATTCTTTCAGTTCCGGAAGGAGATGATAAACCA
CTTAAGTATCCGCTTATGTTTGAGAAGTGTCAGGTTGTGATTATTAATAAGATTGATTCCATGCCGGCATTTGATTTTGA
TATGGATAGGGTTAAGAATAATATTGCAATGAGAAATCCGGAAGCAGTAGTTTTCCCTATTTCTGCAAGAACAGGAGAAG
GAATGGATGCATGGTGCGAATGGCTAAAAGACAATGCAGCACAATATTTAGGAGAGAACCAGAATGGGTAA

Upstream 100 bases:

>100_bases
TTGAAGAGGAGAATGAAAGGGCAGTGAAAGCATATAAGAAGGCTGGATTTGAGAAGCTTGGTTATGTGCAGATGGCTAAG
GATTACTAATTGGAGTTGTT

Downstream 100 bases:

>100_bases
TGTAAGAGTAATTGATGTACATCAGAGTGTTTATGCAGTTAATGATGAGATAGCTGCTAAGACAAGGGCAAGACTTAAGG
AAGAGAAAACATTTATGGTT

Product: hydrogenase nickel incorporation protein HypB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 236; Mature: 236

Protein sequence:

>236_residues
MNNSYNERSMKIIDLKKTILEDNDADADRLREELKKKNVFYMNVMSSPGSGKTTTIVGTIERLRKHFNIGVMEADIDSDV
DALTVAKAGVSAVQLHTGGMCHLDADMSRQGMAALEAEADNALDFIILENVGNLVCPAEFDTGACLNVAILSVPEGDDKP
LKYPLMFEKCQVVIINKIDSMPAFDFDMDRVKNNIAMRNPEAVVFPISARTGEGMDAWCEWLKDNAAQYLGENQNG

Sequences:

>Translated_236_residues
MNNSYNERSMKIIDLKKTILEDNDADADRLREELKKKNVFYMNVMSSPGSGKTTTIVGTIERLRKHFNIGVMEADIDSDV
DALTVAKAGVSAVQLHTGGMCHLDADMSRQGMAALEAEADNALDFIILENVGNLVCPAEFDTGACLNVAILSVPEGDDKP
LKYPLMFEKCQVVIINKIDSMPAFDFDMDRVKNNIAMRNPEAVVFPISARTGEGMDAWCEWLKDNAAQYLGENQNG
>Mature_236_residues
MNNSYNERSMKIIDLKKTILEDNDADADRLREELKKKNVFYMNVMSSPGSGKTTTIVGTIERLRKHFNIGVMEADIDSDV
DALTVAKAGVSAVQLHTGGMCHLDADMSRQGMAALEAEADNALDFIILENVGNLVCPAEFDTGACLNVAILSVPEGDDKP
LKYPLMFEKCQVVIINKIDSMPAFDFDMDRVKNNIAMRNPEAVVFPISARTGEGMDAWCEWLKDNAAQYLGENQNG

Specific function: Could be involved in nickel binding and accumulation. Binds 3.9 nickel ions per molecule [H]

COG id: COG0378

COG function: function code OK; Ni2+-binding GTPase involved in regulation of expression and maturation of urease and hydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hypB/hupM family [H]

Homologues:

Organism=Escherichia coli, GI1789082, Length=213, Percent_Identity=41.3145539906103, Blast_Score=167, Evalue=6e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003495
- InterPro:   IPR004392
- InterPro:   IPR012202 [H]

Pfam domain/function: PF02492 cobW [H]

EC number: NA

Molecular weight: Translated: 26029; Mature: 26029

Theoretical pI: Translated: 4.39; Mature: 4.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
5.5 %Met     (Translated Protein)
7.6 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
5.5 %Met     (Mature Protein)
7.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNSYNERSMKIIDLKKTILEDNDADADRLREELKKKNVFYMNVMSSPGSGKTTTIVGTI
CCCCCCCCCEEEEEHHHHHHCCCCCCHHHHHHHHHHCCEEEEEEECCCCCCCEEEEHHHH
ERLRKHFNIGVMEADIDSDVDALTVAKAGVSAVQLHTGGMCHLDADMSRQGMAALEAEAD
HHHHHHCCCCEEEECCCCCCHHHHHHHCCCEEEEEECCCEEEECCCCCCCCCEEEECCCC
NALDFIILENVGNLVCPAEFDTGACLNVAILSVPEGDDKPLKYPLMFEKCQVVIINKIDS
CCEEEEEEECCCCEEECCCCCCCCEEEEEEEECCCCCCCCEECCEECCCCEEEEEECCCC
MPAFDFDMDRVKNNIAMRNPEAVVFPISARTGEGMDAWCEWLKDNAAQYLGENQNG
CCCCCCCHHHHHCCCEECCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MNNSYNERSMKIIDLKKTILEDNDADADRLREELKKKNVFYMNVMSSPGSGKTTTIVGTI
CCCCCCCCCEEEEEHHHHHHCCCCCCHHHHHHHHHHCCEEEEEEECCCCCCCEEEEHHHH
ERLRKHFNIGVMEADIDSDVDALTVAKAGVSAVQLHTGGMCHLDADMSRQGMAALEAEAD
HHHHHHCCCCEEEECCCCCCHHHHHHHCCCEEEEEECCCEEEECCCCCCCCCEEEECCCC
NALDFIILENVGNLVCPAEFDTGACLNVAILSVPEGDDKPLKYPLMFEKCQVVIINKIDS
CCEEEEEEECCCCEEECCCCCCCCEEEEEEEECCCCCCCCEECCEECCCCEEEEEECCCC
MPAFDFDMDRVKNNIAMRNPEAVVFPISARTGEGMDAWCEWLKDNAAQYLGENQNG
CCCCCCCHHHHHCCCEECCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8326860 [H]